A protocol for the preparation and evaluation of placenta-tropic mRNA LNPs for treating pregnancy disorders. 🧪 @natprot.nature.com

This protocol offers a versatile approach to the precise targeting of nucleic acid-containing lipid nanoparticles to cell types or tissues, such as liver- and lung-targeting LNPs in vivo and ex vivo human primary T cell-targeting LNPs. 🧪 @natprot.nature.com

Real-time EEG–TMS enables the timing of TMS pulses relative to target oscillatory brain states with millisecond-precision @paolobelardinelli.bsky.social 🧪🧠 @natprot.nature.com

Single-cell ultra-high-throughput multiplexed sequencing leverages a combinatorial indexing strategy in which chromatin and mRNA are indexed in situ using sample-specific barcodes, and encapsulated into microfluidic droplets. @tommytang.bsky.social 🧪 @natprot.nature.com

A protocol for 3D aligned nanofiber scaffolds provides spatial support for the repair of trauma-induced tissue defects 🧪 #materials @natprot.nature.com

Volumetric DNA microscopy encodes spatial information into DNA sequences rather than photons, enabling template-free reconstruction of 3D morphology and gene expression patterns in intact specimens through computational analysis of sequencing data. @jaweinst.bsky.social 🧪🔬 @natprot.nature.com

A protocol for determining chemical reactions using acoustic droplet ejection mass spectrometry directly from starting materials enables the fast analysis of hundreds of samples. 🧪⚗️ @natprot.nature.com

Here, a protocol offering a probe-based sonochemical method for rapidly (under 1 h) synthesizing covalent organic frameworks (COFs) in an aqueous environment under ambient atmosphere, avoiding harmful solvents and high-pressure systems. 🧪 #materials @natprot.nature.com

Microplastic aging is a dynamic process that depends on the chemical composition, size and shape of the material, and its environmental conditions. Here, a protocol to characterize aged microplastics in soil, water, air and organic material. 🧪⚗️ @natprot.nature.com

Here, a protocol offering a high-throughput strategy to identify endogenous biomolecular condensates and phase-separating proteins by integrating sucrose density gradient centrifugation with quantitative mass spectrometry. 🧪⚗️ @natprot.nature.com www.nature.com/articles/s41...

“I treated that access as something to be quiet about rather than discussed freely with others” Peter Attia, explaining how to move in philanthropic circles and raise funds. Desk reject. 🧪 #academicsky

Where is the #Arabidopsis community on @bsky.app? Any #Brassica fans? See our recent protocol covering design principles and considerations for building and testing programmable and reversible CRISPRi-based gene circuits in plants. 🧪 #plantscience @natprot.nature.com @ryanlister.bsky.social

A Protocol for characterizing the interactions between tumors and neurons in patient-derived GBM organoids: 🧪🧠 @natprot.nature.com

A workflow for 3D correlative cryo-fluorescence light microscopy-FIB-ET that streamlines fluorescence light microscopy-guided FIB milling, improving throughput while preserving both structural and contextual information 🧪🔬 #imaging @natprot.nature.com @erwright73.bsky.social

Interested in magnetoencephalography? This protocol covers the planning of MEG studies, the execution of pilot experiments, the procedure implementation and the quality control required to ensure consistency in training and data acquisition and preprocessing. 🧠🧪 #neuroimaging @natprot.nature.com

Check out our recent protocol on a framework for statistical testing of brain dynamics based on an open-source Python package built on a generalization of the hidden Markov model (HMM). 🧪🧠 #neuroimaging @natprot.nature.com

A guide for designing and fabricating an acoustic separation device, including channel fabrication, the experimental setup and acoustic field optimization, as well as the biofluid preparation for the isolation of viruses, extracellular vesicles and other nanoparticles. 🧪 @natprot.nature.com

Did you catch our recent Protocol describing the inference of cell fate from single-cell RNA sequencing data using CellRank? 🧪 @natprot.nature.com @fabiantheis.bsky.social

🧪 1.5 years ago I asked whether the rise of research in China was going to influence the biomedical/healthcare sector, following similar success in other areas. Find below an update:

In this Protocol you will find comprehensive guidance for researchers, including those with limited electron microscopy experience, on the effective use of genetically encoded, multichannel EM-capsulin reporters for both fluorescence microscopy and EM workflows. @natprot.nature.com 🧪 🔬

Happy to be attending the Cellular therapy and immunotherapy conference 2025 in Hangzhou. I’m looking forward to learning about the latest developments in the field! 🧪 #medsky mm.sciconf.cn/en/minisite/...

Longer walks linked with less mortality and cardiovascular risk compared with short bouts of physical activity @annalsofim.bsky.social www.acpjournals.org/doi/10.7326/...

Something to think about when talking to your favourite AI system: How to write effective prompts for large language models www.nature.com/articles/s41...

🧵🚨 The UK’s independent scientific bodies are highly vulnerable to politicisation - over the past 5 months I've been working with @martinmckee.bsky.social to map out their vulnerabilities and it's not good news. Today our report is published! www.ucl.ac.uk/policy-lab/n... 1/11

After a long pause, I've just done another England Covid & NHS update as we head into the autumn/winter season. TLDR: we're in a significant Covid wave now, the NHS is stretched, get boosted if you're eligible. christinapagel.substack.com/p/england-is...

🧠📈 Nerd-chic 💙

Congratulations to all three winners! Susumu Kitagawa, Richard Robson and Omar M. Yaghi “for the development of metal-organic frameworks” We are honoured to have a Nature Protocol from Prof. Yaghi rdcu.be/eJ0Es www.nature.com/articles/s41...

BREAKING: The Nobel Prize in Chemistry has been awarded to Susumu Kitagawa, Richard Robson and Omar M. Yaghi “for the development of metal-organic frameworks” Stay tuned for more. #NobelPrize

Good to know: There is a free online training course for peer review from Nature Masterclasses masterclasses.nature.com/focus-on-pee...

When discussing content proposals for @natprot.nature.com with potential authors I often end up covering the wide reach of our journal. Often researchers are interested in examples of articles which have seen wide uptake, accesses and use from the scientific community. Here, a🧵of readcube links: 🧪🎓

Bioinspired in-sensor computing devices can process information at sensory terminals by leveraging physical principles, thereby reducing latency and energy consumption during computation while simultaneously enhancing the efficiency of data processing and real-time analysis. 🧪 @natprot.nature.com

How can we make sure that studies using transcranial electric stimulation (tDCS, tACS, etc.) are interpretable and reproducible? This new work is a consensus stement about what information is needed when reporting tES studies: www.nature.com/articles/s41...

Transcranial electrical stimulation (tES) has gained substantial momentum as a research and therapeutic tool; however, it suffers from challenges related to reproducibility and quality assessment due to the absence of standardized reporting practices. @natprot.nature.com 🧪🧠 #imaging #radiology

In this Protocol, Guosheng Song and co-authors provide instructions for the synthesis of trianthracene derivative (TD) nanoparticles with ultrasound-induced luminescence properties. @natprot.nature.com 🧪🔬 #imaging

The coupling of ion-exchange chromatography with mass spectrometry via electrochemical ion suppression creates a technique for the analysis of highly polar and ionic compounds with applications in environmental chemistry, food chemistry, forensics, cell biology and, more recently, metabolomics. 🧪

The versatility of lanthanide-doped near-infrared (NIR, 700–1,700 nm) luminescent nanoparticles makes them valuable tools across scientific and technological fields, from bioimaging to information security. @natprot.nature.com 🧪 #materials #imaging

Thermal scanning-probe lithography (tSPL) is a fabrication technique which can pattern mathematically defined landscapes with nanoscale dimensions. This protocol describes how to structure and optimize nanomaterials with tSPL with a software package to analyze topographic data. @natprot.nature.com 🧪

This protocol provides the instructions to automating key steps, including data preprocessing, model fine-tuning and evaluation for single-cell generative pretrained transformer (scGPT) using Python function wrappers within computing clusters and Jupyter notebooks. @natprot.nature.com 🧪

"The world has changed" say researchers who decided to leave the US. Having relocated to China a year ago with my family, the stories in this article resonate with us. Stereotypes were never true in the first place, and today we get to embrace that change and help write a new chapter.👏 @nature.com

Lymph node metastatic spread is hard to study due to the unpredictable characteristics of tumour cells' migration. The @retickerflynn.bsky.social lab here explains how to control LN metastases in mice in a spontaneous or a spatially constrained experimental model @natprot.nature.com #medski 🧪

Noble metal aerogels (NMAs) combine the physicochemical properties of nanostructured metals with the self-standing porous architecture of aerogels. NMAs have potential in #catalysis, #sensing and other applications in fundamental and applied sciences. @natprot.nature.com 🧪 #materials

I don’t usually do wordle anymore but saw Wordle X was trending over there… what’s the big deal? 🤣🤣🤣 Wordle 1,452 6/6 ⬜⬜🟨⬜⬜ 🟨⬜🟨⬜🟩 🟩🟩⬜⬜🟩 🟩🟩⬜⬜🟩 🟩🟩⬜⬜🟩 🟩🟩🟩🟩🟩

Cancer Research UK today published a report which highlights changes in cancer survival rates over the last 50 years. Many headlines (like this one from the Guardian) have chosen to report the ‘doubling of cancer survival rates’ since the 1970s. But that's misleading... 1/18

If you are interested in radiosynthesis and clinical quality control procedures then you can check out our new protocol on clinical grade lutetium-177-labeled theranostic agents 🧪 #medsky #radsky #pharmasky #pharmasky @natprot.nature.com

Simply staggering and a devastating blow to scientific and medical research in the US

Sources: Harvard University just filed a motion related to the Trump administration's revocation certification to accept foreign students. This revocation affects 7,000 visa holders.

In this Perspective, we discuss how the experience from Independent SAGE may help develop science advice groups for future public health emergencies and improve science communication efforts with the public. The article is now free to access!

Our May issue is live and includes an interesting piece written by @independentsage.bsky.social and published in December, discussing their mission and organization during Covid rdcu.be/d3n6s 🧪 @natprot.nature.com

The reason why @independentsage.bsky.social were able to identify and respond to the Immensa testing scandal was because we were looking at the data and listening to local communities who were experiencing the problem. #CovidInquiry