Today, our animation synthesizing decades of research on actin-mediated endocytosis in budding yeast was published: journals.biologists.com/jcs/article/... The result of a fantastic Iwasa-Drubin lab collaboration. @margotriggi.bsky.social @jiwasa.bsky.social movie.biologists.com/video/10.124...

Un très grand plaisir d'avoir pu enfin (après 5 ans) voir @elisabethbik.bsky.social en face à face à l'UNIL. Elle y donne en plus un séminaire aujourd'hui.

All credits to the dreamteam that made this possible! what a pleasure and honor working with these people! 🥰 @diorgeps.bsky.social @mhakala.bsky.social @juanmagararc.bsky.social @joshuatran.bsky.social @mudgal17.bsky.social @Carlos Marcuello @Andrea Merino

The crucial test: We fused Heimdall Hofund to a fission-defective yeast ESCRT-III protein (Did2). This chimera restored Mup1 trafficking to vacuoles back to wt! A short amphipathic helix, present in Asgard and retained as fragments in eukaryotes, acts as a minimal membrane fission trigger!

Eukaryotic ESCRT-IIIA paralogs, known to form heteropolymers, retain Hofund elements at their N-termini. In yeast, mutating these elements blocks ESCRT-III-dependent Mup1 transport to vacuoles. So these elements matter in eukaryotes too.

Is this Asgard-specific, or conserved with their eukaryotic paralogs? Hard to tell, since the exact molecular mechanism of fission by eukaryotic ESCRT-III remains blurry, probably due to its complexity. Let’s figure it out!

Meet Hofund, the N-terminal amphipathic helix of 15 aa in Heimdall ESCRT-IIIA (named after Heimdall’s sword). How do we know Hofund is the molecular trigger for fission? Remove Hofund → ESCRT-IIIA loses fission activity. Add Hofund alone → uncontrolled fission.

Through membrane fission! We show in vitro that the Asgard Heimdallarchaeota (Heimdall) ESCRT-IIIA subunit is inherently capable of triggering fission upon subunit turnover driven by ATP hydrolysis by Vps4. And the key question: what actually destabilizes the membrane when ESCRT-IIIA turns over?

In a recent work, @buzzbaum.bsky.social and colleagues showed an Asgard archaeon with internal vesicles. www.biorxiv.org/content/10.1... How might Asgard ESCRT-III have contributed to compartmentalization?

Fantastic work from Javier @javierespadas.bsky.social in collaboration with @buzzbaum.bsky.social and @kaksonen.bsky.social labs, thank you Chris Toret, thank you Diorge @diorgeps.bsky.social!

Thanks to all the team at @rouxlab.bsky.social and abroad: @pauguillamat.bsky.social @caterinatomba.bsky.social @giodang.bsky.social @colomlab.bsky.social @lizhinde.bsky.social @javierespadas.bsky.social and many more

Overall, the work shows that tension gradients arise from the combination of actin dynamics and strong cell–substrate adhesion, rather than from migration itself. Link: rdcu.be/eRTQA

We accompanied this dynamic live Flipper-TR FLIM imaging with lipid diffusion analysis, spatial lipidomics (shown below), and cool in vitro reconstitutions of tension gradients using supported lipid bilayers that are expanding

A key result: adherent cells maintain long-range membrane tension gradients even when they are not migrating! (micropatterned cells below) In contrast, non-adherent migrating cells *do not* show these gradients.

Microfluidic pumping with active nematics! Self-organized and self-sustained. No external pumps. @pnas.org @ub.edu www.pnas.org/doi/10.1073/... #ActiveMatter #Microfluidics #SoftMatter

Maria Tettamanti shows that inhibition of TORC2 by the small amphipath PalmC induces the TORC2-dependent internalization of sterols. Congrats to Maria and all coworkers involved, and to EMBOj for the efficient review process: use review commons! @biology-unige.bsky.social @sciencesunige.bsky.social

Etienne @membramics-lab.bsky.social is very happy to visit @rouxlab.bsky.social in Geneva! great people, great projects, good memories...

‪ Congratulations @centriolelab.bsky.social @dudinlab.bsky.social and @gautamdey.bsky.social for your publication in @cp-cell.bsky.social: Charting the landscape of #cytoskeletal diversity in #microbial eukaryotes #Expansion #Microscopy @sciencesunige.bsky.social www.unige.ch/medias/en/20...

*ALL*source data and Python notebooks are available (you can reproduce all figures!) 🔗 doi.org/10.6084/m9.f... We hope this helps make lifetime analysis transparent and comparable across setups. 📄 www.biorxiv.org/content/10.1... @rouxlab.bsky.social #FLIM #FlipperTR #Microscopy #OpenScience

🚨 New preprint out! “Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies. www.biorxiv.org/content/10.1...

I am pleased to 📣 today’s superb thesis defense by Christian Schroer on “Chemical strategies for studying lipid signaling and transport”. Part of his work was recently published in the JLR; the other part will follow soon. A very proud and somewhat tipsy “Doktorvater” 🥂

Undergraduate/internship/master's thesis position: jobs.helsinki.fi/job-invite/4...

PhD student position: jobs.helsinki.fi/job-invite/4...

I have a PhD student and an undergrad student positions open at the Institute of Biotechnology @helsinki.fi We are using reconstitution, cell and structural biology to study protein-membrane interactions and membrane microdomain formation. Links to calls below! Feel free to drop a message and share!

"Impaired hematopoiesis and embryonic lethality at midgestation of mice lacking both lipid transfer proteins VPS13A and VPS13C" out now in PLOS Biology journals.plos.org/plosbiology/...

Order matters! Aurelien Roux @rouxlab.bsky.social studied the formation of multi-cellular tornado-like structures and regeneration of heads in hydra to share with us how stress fields, topology and geometry of initial tissue organization set the sequence of morphogenesis. #MBIMPG2025

Previous preprint is now published in RSC Chemical Biology. If you are interested in plasma membrane labeling, see the paper! 👇 pubs.rsc.org/en/content/a...

Nice discussions after the talk given online yesterday at cytophys.org, presenting the work of @pauguillamat.bsky.social @clairedessalles.bsky.social @yamini-ravichan.bsky.social @oriolmb.bsky.social and Tithi Mandal. Available on the lab youtube channel at: youtu.be/g-7HomnPdtk

Ever wished to become an actin star? 😎 Then try getting suspended above a non-adhesive substrate. www.nature.com/articles/s41...

Hello microscopy and imaging experts and enthusiasts! We are looking for a staff scientist to join us here in Geneva to work on microscopy and image analysis projects! See the link for the job announcement (in French and in English). Please apply and/or repost! jobs.unige.ch/www/wd_porta...

The @embo.org ESCRT meeting was packed with membrane remodeling from every branch of the tree of life🌳. Some awesome science 🧪 highlights in the next posts 🧵. But a first impression… the venue!! Wow, Caux castle up a cogwheel train, overlooking Lac Léman! Perfect! 🌄 Thank you @rouxlab.bsky.social 🤗

What a fantastic ESCRT meeting, the field is going strong and full of exciting science. Many thanks to @rouxlab.bsky.social @cellarchlab.com @jujumathieu.bsky.social @magromayor.bsky.social @majarad.bsky.social for organising such a a great event

🤩 Last week I successfully defended my PhD! It was such a special day, made even better by having friends, family, and colleagues there with me. Thanks for all the support and kind words! Huge thanks to my jury members for the thoughtful feedback and conversations. @soldatilab.bsky.social

Thank you for this opportunity @the-node.bsky.social to let me share the behind the story of my postdoc article from the @rouxlab.bsky.social here’s to squishing jellies!!

This was a truly exciting talk from Aurelien Roux @rouxlab.bsky.social from Univ. Geneva 👏👏. From his landmark work on membranes to tissues now, the continuing theme is the role of geometry, topology and curvature in morphogenesis. Thanks for visiting us 🤩

The amazing tour de force of the great @lcferme.bsky.social is now out in its final form in @ScienceAdvances (not on Bluesky???). It was hard work and all the conceptual advance was her doing. Check it out: www.science.org/doi/epdf/10....

Just one week to go ⏳ until the "Cellular Interfaces Across Scales: From Molecules to Tissue Dynamics" meeting @sfb1348.bsky.social @uni-muenster.de We can't wait to see you there! crc1348-conference.ycode.site #DynamicCellularInterfaces #Münster

The lab is on a roll. 2nd preprint in as many weeks! High levels of Cdc42 GTPase underlie an all-or-none decision to fuse. Great team effort led by PhD students @sajjitasaha.bsky.social and @aiswaryasajeevan.bsky.social www.biorxiv.org/content/10.1... @biology-unige.bsky.social

New lab preprint! High resolution mapping of the actin fusion focus reveals myosin V-dependent transport of formin for actin aster compaction. A very cool work led by @valthomas.bsky.social and nice collaboration with Andrea Picco and @kaksonen.bsky.social! www.biorxiv.org/content/10.1...

#newPI and time is running. It has already been a month since I officially joined the University of Warwick as Assistant Professor. I am excited to announce and build the Fluorescence and Membrane Dynamics (FMD) Lab: warwick.ac.uk/fac/sci/med/...

"The bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage" out now in Nature Cell Biology rdcu.be/ehbaL

Abstract submissions for short talks are still opened until April 20th for the EMBO Escrt meeting 2025. There are plenty of options for short talks, please apply online at meetings.embo.org/event/25-escrt

Thats the proof, that PI to PI knowledge transfer can still work (for now😂). Yay very happy.

Interested in how cells respond when squeezed? Check the review paper just published by Laura Faure, @valeriaventurini.bsky.social and myself on this. Really thorough and clear revision by Laura and Valeria!

Grateful for the opportunity to present my work last week at #EMBOcellBio24. A big thank you to @humantechnopole.bsky.social and @embo.org for organizing such an inspiring event with an impressive lineup of speakers in the stunning Austrian landscape!

A short reminder that registrations for the EMBO ESCRT meeting 2025 in Montreux June 23rd-June27th are still opened! Please register online at meetings.embo.org/event/25-escrt

Paper 4: @rouxlab.bsky.social lab studied contractility-curvature dynamics in/on cell tubes ➡️ impacting tissue remodeling with proliferation and extrusion.

Cloud attack on Matterhorn today

Uh oh...... Pubmed appears to be down....

In this interview, Dominique Bergmann @stanfordstomata.bsky.social talks about her research in stomatal development, her role as an Editor at Development, and how her passion for comparative biology has influenced her career: https://doi.org/10.1242/dev.204667