Secretome translation shaped by lysosomes and lunapark-marked ER junctions www.nature.com/articles/s41...

Neuronal compartmentalization results in impoverished axonal mitochondria https://www.biorxiv.org/content/10.1101/2025.10.27.684882v1

I am thrilled to share our latest story led by the incredibly talented Brooke D’Arcy and Camila Musso. We discover a rich world of local gene expression in radial glia, essential neural and glial precursors, and develop a new method for sub-cellular mRNA manipulation. www.biorxiv.org/content/10.1...

🚀 Our new paper is out @natmethods.nature.com! Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells. 🔗 www.nature.com/articles/s41... 🧬 www.addgene.org/John_Ngo/

Wow, this paper provides crucial understanding of local translation and processing of transmembrane proteins in axons, one of the big mysteries that have haunted the field for a long time!!!!!!!! Fantastic!!!!!!!! Nguyen et al., 2025, bioRxiv 2025.09.09.674816ff. -- doi.org/10.1101/2025...

✨ Post-doc opportunity in axon biology, funded by #ATIP-Avenir! 👀 We are looking for an enthusiastic post-doc to join our team at @ibdm.bsky.social to explore translation dynamics in axon regrowth, using live imaging approaches. Apply at: emploi.cnrs.fr/Offres/CDD/U... Thank you for sharing! 👋 🙏

Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.1.... We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.

I'm thrilled to share our work from @weissmanlab.bsky.social (www.sciencedirect.com/science/arti...). We developed LOCL-TL, an optogenetic approach for monitoring localized translation in mammalian cells. LOCL-TL revealed two distinct strategies for mitochondrially localized translation.

Cryo-EM structures of Egl-BicD-RNA complexes reveal how diverse mRNAs are selected for subcellular localization https://www.biorxiv.org/content/10.1101/2025.08.02.668268v1

If you’ve ever wondered how cells tune positioning of organelles in response to cell shape change, and what happens when this goes wrong, please check out our new manuscript – mRNA trafficking controls cell-size-scaled organelle dynamics! www.nature.com/articles/s41...

RNA can control cell migration by acting at focal adhesions www.lifescienceeditors.com/2025/06/24/r...

📢 For all HaloTag lovers💙💚💛❤️💜🩶 we are excited to share that our work, in which we developed a new biotin-HaloTag ligand, is now published in @jcb.org! Full link here: rupress.org/jcb/article-...

Online Now: FMRP drives mRNP targets into translationally silenced complexes Online now:

Kicking off the lab BlueSky with this picture of a happy hairy growing #axon! Looking forward to connecting with science chats and to growing research ideas 🧠🔬✨

Optogenetic storage and release of protein and mRNA in live cells and animals | Nature Communications share.google/gz8nYXZ3hUVh... #spatialbiology #imagingtools #fluorescencemicroscopy

Hello! Ever wonder what's "talking to" your favorite transcript, but were too scared to ask? In our review in @cp-cellreports.bsky.social, @mardakheh.bsky.social and I highlight new RNA-focused tools for discovering RNA interactions across organizational scales. Checkit! tinyurl.com/ydn6e3ac

Researchers have found that turning down a single gene, EMC10, can boost memory and brain function in mice with a genetic condition linked to #schizophrenia. The findings offer hope for future treatments for 22q11.2 deletion syndrome.

Neuronal potassium channel activity triggers initiation of mRNA translation through binding of translation regulators. www.science.org/doi/10.1126/...

Programmable control of spatial transcriptome in live cells and neurons. www.nature.com/articles/s41...

A 🧵 to walk those interested through this paper...

Murphy et al. @amsterdamumc.bsky.social show that MUNC13 is essential for dense core vesicle secretion, while RIM prevents MUNC13 degradation. Key membrane-binding domains of RIM & MUNC13 play differing roles, revealing distinct mechanisms from synaptic vesicle release. rupress.org/jcb/article/...

What drives cytoplasmic mRNA organization? We created unbiased, genome-wide maps of mesoscale RNA-RNA spatial proximity, revealing impact of encoded protein function. Fantastic work from @lindsayabecker.bsky.social @sofiquinodoz.bsky.social @davidaknowles.bsky.social www.biorxiv.org/content/10.1...

Excited for our latest work! We developed nanobinders for the luminal domain of Synaptotagmin 1 (calcium sensor for synaptic vesicles). Thanks among others to @verstrekenlab.bsky.social @opazo.bsky.social @meunierlab.bsky.social @volkerhaucke-lab.bsky.social www.biorxiv.org/content/10.1...

In getting too excited about the front cover, I forgot to link to the actual paper 🤦‍♂️ Here it is: Dynamic modulation of the motor neuron translatome during developmental synapse elimination | Science Signaling www.science.org/doi/10.1126/...

Ever wondered how several individual RBPs cooperate to repress translation? Lead by @marcopayr.bsky.social and in great collaboration with @hennig-lab.bsky.social, we simultaneously tracked the binding of several proteins to single mRNA molecules in real-time! www.biorxiv.org/content/10.1...

Honored to help enable two back-to-back papers in @natureneuro.bsky.social; first DELTA www.nature.com/articles/s41... with collaborators at @hhmijanelia.bsky.social and then EPSILON www.nature.com/articles/s41... with @adamezracohen.bsky.social; @dyerfulchymist.bsky.social is always in the mix!

It's out! www.biorxiv.org/content/10.1...

A nearly 9 year effort to design and implement new CLIP-based technologies to define multimeric protein assemblies on RNA. Enables ‘watching’ of RNP assemblies inside living cells Special congrats to Luca Ducoli, Brian Zarnegar, and Paul Khavari @nature.com www.nature.com/articles/s41...

We are recruiting!! If you are looking for a post-doc (ephys, slice imaging) or a PhD position (molecular neuroscience), are interested in Axon Initial Segment plasticity and want to join a nice team in a great environment, have a look! And, that's us 👇

ANXA11 biomolecular condensates facilitate protein-lipid phase coupling on lysosomal membranes! Annexin A11 tethers RNP granules to membranes in a Ca2+ dependent manner. @abelljonny.bsky.social, @michael-e-ward.bsky.social, et al. www.nature.com/articles/s41...

Axonal RNA localization is essential for long-term memory www.nature.com/articles/s41...

Whoa - are a significant proportion of sporadic ALS/motor neuron disease cases a mitochondrial DNA disease?! #mitochondria www.nature.com/articles/s41...

The first review paper from our lab is out!! @viviennebauer.bsky.social discussed the rapidly emerging knowledge on (the interplay between multiple) organelles and neuronal mRNA localization. Hope you enjoy the read! www.sciencedirect.com/science/arti...

Our CNS regeneration work has now been published in @PNAS. It was a huge undertaking in collaboration with @Twiss @Benowitz @WardLab_Emory @KristyWelshhans @Marktuszynski @jenniferDulin @ArthurEnglish @JohnHolule labs. Thank you all, and @MerkinPnnrCtr, for funding. www.pnas.org/doi/10.1073/...

www.science.org/doi/10.1126/... Tour de force by Barna Lab

New preprint from Roz Segal’s group on specialization in local axonal translation www.biorxiv.org/content/10.1...

Excited to share our latest work! We asked if and how TDP-43 regulates RNA transport in neurons and if this is misregulated in ALS. We found that it works to keep RNAs *out* of neurites, and that loss of TDP-43 activity results in specific RNAs aberrantly accumulating there.

Save the date for the next edition of this wonderful meeting. We look forward to seeing you in Portugal!!

www.nature.com/articles/s41... POTATOMap is officially out today! (Even tho it says 2024Dec30… yeah long story😂) Thank you to all the authors and everyone involved! I hope we have provided something useful and maybe leave a little (just a little) impact in your research.

So cool! rdcu.be/eboxL

Our review "Neuronal autophagy in the control of synapse function" is already available online. Have a look! www.sciencedirect.com/science/arti...

RNA promotes synapsin coacervation and modulates local translation https://www.biorxiv.org/content/10.1101/2025.01.29.635107v1

Everything you always wanted to know about the Axon Initial Segment might be here: authors.elsevier.com/sd/article/S...

The dynamic axon initial segment: From neuronal polarity to network homeostasis www.cell.com/neuron/fullt...

My lab at CUHK (QS: 36th) is hiring! We are looking for motivated Research Assistants, PhD students (urgent🚨) and Postdocs who love solving problems. To understand the mysterious brain, we use a range of fun techniques. Please spread the word and get in touch if you are interested!

Amazing stuff from @marvintanenbaum.bsky.social! Long-term imaging of individual ribosomes reveals ribosome cooperativity in mRNA translation: Cell www.cell.com/cell/fulltex... #RNAsky #RNABiology

🚨LAST WEEK TO APPLY🚨 We are recruiting postdocs to help us understand HSV-1 latency using #stemcell models. #virology You will be part of a collaborative team & receive extensive mentorship to develop your own independent line of research. Please share! careers.humantechnopole.it/o/postdoc-ha...