Come and join our team @humantechnopole.bsky.social! We are recruiting a Senior Technician. You will be part of a collaborative and diverse team working on human stem cell biology, precise genome-engineering, and virology. More info: careers.humantechnopole.it/job/Senior-T... Please share.

Cells come in many shapes and sizes, with diverse physiological functions. But how do #organelles and their interaction networks remodel during #differentiation of stem cells into different cell types? Here’s what we discovered about neuronal differentiation: 1/13

Active transport of tRNAs facilitates distributed protein synthesis https://www.biorxiv.org/content/10.64898/2026.01.26.698744v1

Very happy to share our new paper in #ScienceSignaling revealing that importin β1 isn’t just important for nuclear transport or neuronal growth. We now show it is key for presynaptic plasticity in the hippocampus, local translation, and memory! www.science.org/doi/10.1126/...

New preprint from my lab!!! We took advantage of the MitoTag mouse line to study what mRNAs associate with mitochondria in axons in vivo, and we were in for a surprise! Other than our favorite Pink1, mainly cytoskeletal mRNAs hitch a ride and pave the way for axon growth. shorturl.at/rYe1U

Dynamic subtype- and context-specific subcellular RNA regulation in growth cones of developing neurons in the cerebral cortex. rdcu.be/eXuwg

Secretome translation shaped by lysosomes and lunapark-marked ER junctions www.nature.com/articles/s41...

Neuronal compartmentalization results in impoverished axonal mitochondria https://www.biorxiv.org/content/10.1101/2025.10.27.684882v1

I am thrilled to share our latest story led by the incredibly talented Brooke D’Arcy and Camila Musso. We discover a rich world of local gene expression in radial glia, essential neural and glial precursors, and develop a new method for sub-cellular mRNA manipulation. www.biorxiv.org/content/10.1...

🚀 Our new paper is out @natmethods.nature.com! Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells. 🔗 www.nature.com/articles/s41... 🧬 www.addgene.org/John_Ngo/

Wow, this paper provides crucial understanding of local translation and processing of transmembrane proteins in axons, one of the big mysteries that have haunted the field for a long time!!!!!!!! Fantastic!!!!!!!! Nguyen et al., 2025, bioRxiv 2025.09.09.674816ff. -- doi.org/10.1101/2025...

✨ Post-doc opportunity in axon biology, funded by #ATIP-Avenir! 👀 We are looking for an enthusiastic post-doc to join our team at @ibdm.bsky.social to explore translation dynamics in axon regrowth, using live imaging approaches. Apply at: emploi.cnrs.fr/Offres/CDD/U... Thank you for sharing! 👋 🙏

Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.1.... We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.

I'm thrilled to share our work from @weissmanlab.bsky.social (www.sciencedirect.com/science/arti...). We developed LOCL-TL, an optogenetic approach for monitoring localized translation in mammalian cells. LOCL-TL revealed two distinct strategies for mitochondrially localized translation.

Cryo-EM structures of Egl-BicD-RNA complexes reveal how diverse mRNAs are selected for subcellular localization https://www.biorxiv.org/content/10.1101/2025.08.02.668268v1

If you’ve ever wondered how cells tune positioning of organelles in response to cell shape change, and what happens when this goes wrong, please check out our new manuscript – mRNA trafficking controls cell-size-scaled organelle dynamics! www.nature.com/articles/s41...

RNA can control cell migration by acting at focal adhesions www.lifescienceeditors.com/2025/06/24/r...

📢 For all HaloTag lovers💙💚💛❤️💜🩶 we are excited to share that our work, in which we developed a new biotin-HaloTag ligand, is now published in @jcb.org! Full link here: rupress.org/jcb/article-...

Online Now: FMRP drives mRNP targets into translationally silenced complexes Online now:

Kicking off the lab BlueSky with this picture of a happy hairy growing #axon! Looking forward to connecting with science chats and to growing research ideas 🧠🔬✨

Optogenetic storage and release of protein and mRNA in live cells and animals | Nature Communications share.google/gz8nYXZ3hUVh... #spatialbiology #imagingtools #fluorescencemicroscopy

Hello! Ever wonder what's "talking to" your favorite transcript, but were too scared to ask? In our review in @cp-cellreports.bsky.social, @mardakheh.bsky.social and I highlight new RNA-focused tools for discovering RNA interactions across organizational scales. Checkit! tinyurl.com/ydn6e3ac

Researchers have found that turning down a single gene, EMC10, can boost memory and brain function in mice with a genetic condition linked to #schizophrenia. The findings offer hope for future treatments for 22q11.2 deletion syndrome.

Neuronal potassium channel activity triggers initiation of mRNA translation through binding of translation regulators. www.science.org/doi/10.1126/...

Programmable control of spatial transcriptome in live cells and neurons. www.nature.com/articles/s41...

A 🧵 to walk those interested through this paper...

Murphy et al. @amsterdamumc.bsky.social show that MUNC13 is essential for dense core vesicle secretion, while RIM prevents MUNC13 degradation. Key membrane-binding domains of RIM & MUNC13 play differing roles, revealing distinct mechanisms from synaptic vesicle release. rupress.org/jcb/article/...

What drives cytoplasmic mRNA organization? We created unbiased, genome-wide maps of mesoscale RNA-RNA spatial proximity, revealing impact of encoded protein function. Fantastic work from @lindsayabecker.bsky.social @sofiquinodoz.bsky.social @davidaknowles.bsky.social www.biorxiv.org/content/10.1...

Excited for our latest work! We developed nanobinders for the luminal domain of Synaptotagmin 1 (calcium sensor for synaptic vesicles). Thanks among others to @verstrekenlab.bsky.social @opazo.bsky.social @meunierlab.bsky.social @volkerhaucke-lab.bsky.social www.biorxiv.org/content/10.1...

In getting too excited about the front cover, I forgot to link to the actual paper 🤦‍♂️ Here it is: Dynamic modulation of the motor neuron translatome during developmental synapse elimination | Science Signaling www.science.org/doi/10.1126/...

Ever wondered how several individual RBPs cooperate to repress translation? Lead by @marcopayr.bsky.social and in great collaboration with @hennig-lab.bsky.social, we simultaneously tracked the binding of several proteins to single mRNA molecules in real-time! www.biorxiv.org/content/10.1...

Honored to help enable two back-to-back papers in @natureneuro.bsky.social; first DELTA www.nature.com/articles/s41... with collaborators at @hhmijanelia.bsky.social and then EPSILON www.nature.com/articles/s41... with @adamezracohen.bsky.social; @dyerfulchymist.bsky.social is always in the mix!

It's out! www.biorxiv.org/content/10.1...

A nearly 9 year effort to design and implement new CLIP-based technologies to define multimeric protein assemblies on RNA. Enables ‘watching’ of RNP assemblies inside living cells Special congrats to Luca Ducoli, Brian Zarnegar, and Paul Khavari @nature.com www.nature.com/articles/s41...

We are recruiting!! If you are looking for a post-doc (ephys, slice imaging) or a PhD position (molecular neuroscience), are interested in Axon Initial Segment plasticity and want to join a nice team in a great environment, have a look! And, that's us 👇

ANXA11 biomolecular condensates facilitate protein-lipid phase coupling on lysosomal membranes! Annexin A11 tethers RNP granules to membranes in a Ca2+ dependent manner. @abelljonny.bsky.social, @michael-e-ward.bsky.social, et al. www.nature.com/articles/s41...

Axonal RNA localization is essential for long-term memory www.nature.com/articles/s41...

Whoa - are a significant proportion of sporadic ALS/motor neuron disease cases a mitochondrial DNA disease?! #mitochondria www.nature.com/articles/s41...

The first review paper from our lab is out!! @viviennebauer.bsky.social discussed the rapidly emerging knowledge on (the interplay between multiple) organelles and neuronal mRNA localization. Hope you enjoy the read! www.sciencedirect.com/science/arti...

Our CNS regeneration work has now been published in @PNAS. It was a huge undertaking in collaboration with @Twiss @Benowitz @WardLab_Emory @KristyWelshhans @Marktuszynski @jenniferDulin @ArthurEnglish @JohnHolule labs. Thank you all, and @MerkinPnnrCtr, for funding. www.pnas.org/doi/10.1073/...

www.science.org/doi/10.1126/... Tour de force by Barna Lab

New preprint from Roz Segal’s group on specialization in local axonal translation www.biorxiv.org/content/10.1...

Excited to share our latest work! We asked if and how TDP-43 regulates RNA transport in neurons and if this is misregulated in ALS. We found that it works to keep RNAs *out* of neurites, and that loss of TDP-43 activity results in specific RNAs aberrantly accumulating there.

Save the date for the next edition of this wonderful meeting. We look forward to seeing you in Portugal!!

www.nature.com/articles/s41... POTATOMap is officially out today! (Even tho it says 2024Dec30… yeah long story😂) Thank you to all the authors and everyone involved! I hope we have provided something useful and maybe leave a little (just a little) impact in your research.

So cool! rdcu.be/eboxL

Our review "Neuronal autophagy in the control of synapse function" is already available online. Have a look! www.sciencedirect.com/science/arti...