Cathepsin C–Catalyzed Ligation Generates Intralysosomal Amyloid Fibrils from Dipeptide Esters www.biorxiv.org/content/10.6...

Check out the newest work from our, from Fabricio Nicola @fabricionicola.bsky.social on mouse jumping and spinal cell types. Excellent collab with @vulcnethologist.bsky.social www.biorxiv.org/content/10.6...

The most impt change at #NIH and to US science this year is bigger than grant cancellations— it’s how the agency is governed. For 75 years NIH has been largely independent of presidential control. That’s changed this year. New piece from me and @nataliebaviles.bsky.social in @nature.com 🧪

A new #ScienceSignaling study offers insights into the KOLF2.IJ cell line, which is derived from induced pluripotent stem cells and has been proposed as a neuronal model for Alzheimer’s disease and related #dementia research. https://scim.ag/4aFNY9O

Sooo happy to share our new paper in @nature.com “CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly.” A terrific collaboration with @arrudalab.bsky.social, led by co–first authors Alyssa Mathiowetz and Emily Maymand. www.nature.com/articles/s41...

Losing sleep over #iPSC transgene silencing after differentiation? Same here. Bright in iPSCs. Gone after differentiation. Our paper in @cp-cellstemcell.bsky.social maps what actually keeps expression on👇 www.cell.com/cell-stem-ce... 🔗 Free access (50 days): www.sciencedirect.com/science/auth...

Thrilled to see our paper out in @cp-cellstemcell.bsky.social!

📣Excited to announce a new pre-print from the lab 📣 Led by my student @storiesofisobel.bsky.social in collab with Hemali Phatnani @nygenome.org We discovered a novel class of TDP-43 dependent cryptic gene-TE spliced transcripts: crypTEs #TEsky #RNAsky #ALS doi.org/10.64898/2026.01.09.698641

Latest preprint from our lab reports that the distinct pH of anterograde (less acidic) and retrograde (more acidic) lysosomal vesicles in the axon depends on assembly of the V1 and V0 domains of the vacuolar H+ ATPase, mediated by the metazoan RAVE complex www.biorxiv.org/content/10.6...

Begging PhD programs to agree on a common app with only letters of rec that writers upload once. These inane likert scales when students are applying to 15-20 programs is destroying my soul

👉 New publication ahead of print from our lab: tinyurl.com/ye7pntte We show that the protein SPG21, mutated in hereditary spastic paraplegia 21, localizes to endolysosomes via RAB7A, where it promotes mTORC1-dependent TFEB phosphorylation, reducing expression of a subset of TFEB regulated genes

Hasegawa-Ogawa, Okano et al. identify a dominant-negative TDP-43 isoform regulated by #ALS-linked #RNA-binding proteins. hnRNP K promotes its expression, while hnRNP A1 and FUS suppress it. rupress.org/jcb/article/... #Disease #Neuroscience #ProteinHomeostasis #RNAbiology

Neuron programming! Pro-neural TFs + 480 morphogen conditions + scRNA-seq --> Diverse iN subtypes of forebrain, midbrain, hindbrain, spinal cord, and PNS. @hsiuchuanlin.bsky.social‬ @jasperjanssens.bsky.social‬ and Treutlein Lab! @science.org www.science.org/doi/10.1126/... #NGN2 #ASCL1

Nature research paper: EndoMAP.v1 charts the structural landscape of human early endosome complexes https://go.nature.com/3Z6mJ1X

Thanks for hosting me - so much fun to meet with everybody at Berkeley!

Despite some ups and downs at NIH, we do have an open position for a postbac and are considering candidates. Our work is at the intersection of neuroscience and AI, and we aim to understand how the brain's densely connected recurrent networks operate. 🧪

Why do lower-resolution images sometimes yield better results in deep learning for bioimaging analyses? 🤔📉 Mariana Ferreira's new preprint on #ReScale4DL explores this paradox and introduces optimal resolution design! @gomez-mariscal.bsky.social brainchild🧑‍🔬✨ Check: www.biorxiv.org/content/10.1...

talkingpointsmemo.com/edblog/the-w... Not nearly enough coverage of this possibility by traditional media.

Explore #Neurodegeneration and Neural-Immune Interactions, June 8-11! Gain insights from experts like @dulaclab.bsky.social and @weissmanlab.bsky.social. Register now to save $200. Postdocs, enjoy an extra $275 off: hubs.la/Q035NHGV0 #KSNeuroImmune25 #KSNeuroDegen25 @michael-e-ward.bsky.social

LRP10 promotes trafficking of progranulin and prosaposin to lysosomes https://www.biorxiv.org/content/10.1101/2025.05.02.651888v1

Important!! 🚨 “Using the rescission process, which requires only a simple majority in the Senate, the Republican majorities can vote to return the unspent funds. That will lop billions off the NIH budget.” 1/2

New at Can We Still Govern: An anonymous NIH employee maps out the GOP budget gameplan to permanently gut our most important science agency. Impoundment, delay and red tape will create artificial "savings" that become the new benchmark for NIH budgets. donmoynihan.substack.com/p/the-nih-bu...

#neuroai #neuroskyence 🧪 #ai

Amazing team science effort, led by Marius Wernig’s group, to develop a silencing-resistant transgene cassette that maintains expression following iPSC differentiation. This was major barrier for iPSC-based experiments. Thanks to CZI for funding the research! www.biorxiv.org/content/10.1...

Losing sleep over #iPSC transgene silencing after differentiation? Same here — too many times😭 So we built a PiggyBac construct that resists silencing. Expression stays ON, even after differentiation. Our #preprint is out👇 🔗 www.biorxiv.org/content/10.1... Supported by @cziscience.bsky.social

Our lab at the NIH (Bethesda, MD) is looking for postdoctoral fellows to join our team studying the molecular mechanisms of protein trafficking and their links to neurodevelopmental disorders starting on or after 10-1-2025. Send your application to [email protected]. Please share and repost!

I’ve always liked Tom Pollard’s framing of biological research: genetics gives you the parts list, biochemistry the functions/reactions and rates, cell biology the localisations and dynamics, and structural biology the fine detail. Put it all together and you get a mechanistic picture. 🤩

Happy to share our new study that was just published in Neuron! www.cell.com/neuron/fullt...

ANXA11 biomolecular condensates facilitate protein-lipid phase coupling on lysosomal membranes! Annexin A11 tethers RNP granules to membranes in a Ca2+ dependent manner. @abelljonny.bsky.social, @michael-e-ward.bsky.social, et al. www.nature.com/articles/s41...

One day left to submit an abstract for short talk consideration at the upcoming Keystone Neurodegeneration meeting (Whistler, BC, June 8-11). We have an amazing set of speakers lined up already - please join us! For more info, see: vimeo.com/1068280559/9...

Whoa - are a significant proportion of sporadic ALS/motor neuron disease cases a mitochondrial DNA disease?! #mitochondria www.nature.com/articles/s41...

Join us this summer in Whistler, BC, Canada, from June 8-11, 2025, for an inspiring meeting on Neurodegeneration, featuring the esteemed @michael-e-ward.bsky.social, & a stellar lineup of speakers. Secure your spot today! hubs.la/Q0323kyP0 #KSNeuroDegen25 #neurodegeneration #neurodegenerativedisease

There are new stable red fluorescent proteins coming, but organic fluorophores are fighting back! Impressive photostability of Phoenix Fluor 555 for live-cell imaging with HaloTag, just out in @naturemethods.bsky.social: doi.org/10.1038/s415...

Fantastic new story from Sami Barmada’s team on TDP-43 autoregulation, with some fascinating insights into how a “short” TDP-43 isoform is involved pubmed.ncbi.nlm.nih.gov/39792557/

🚨 Out now! @marcoyannic.bsky.social and his former colleagues at the Chan Zuckerberg Biohub have unveiled a new study in Cell. The team led by Manuel Leonetti mapped the subcellular distribution of thousands of proteins to create an atlas of the human proteome ➡️ tinyurl.com/ypn65duk @univie.ac.at

Important information for www.addgene.org/browse/artic...

What a story! www.discovermagazine.com/mind/how-bad...

Great piece - love the conceptual distinction between “work” and “work-related activities”.

Bluetorial: Adventures with tenure-Part 2 My experiences on the Johns Hopkins School of Medicine Professorial Promotions Committee with some thoughts about scholarship vs fund raising success, team science, hyperproductivity, and the age distribution of the professoriate.