Only smiles in the tissue culture room from now on 🙂 Find the TK4 plasmids from the Wernig lab here: www.addgene.org/browse/artic...

Have you ever cried in the tissue culture room because your #iPSC transgene got silenced after differentiation? I have. Many, many times. So we built TK4, a PiggyBac vector designed to resist silencing. Now available on @addgene.bsky.social: #239047 / #239046 Would love to hear your thoughts.

Losing sleep over #iPSC transgene silencing after differentiation? Same here. Bright in iPSCs. Gone after differentiation. Our paper in @cp-cellstemcell.bsky.social maps what actually keeps expression on👇 www.cell.com/cell-stem-ce... 🔗 Free access (50 days): www.sciencedirect.com/science/auth...

Thrilled to see our paper out in @cp-cellstemcell.bsky.social!

Our paper on preventing transgene silencing is now online in @cp-cellstemcell.bsky.social! 🔗 Free access (50 days): www.sciencedirect.com/science/auth...

Our paper is (finally) out in Cell today! CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis www.cell.com/cell/fulltex... Great collaborative effort - read more from first author @asamelson.bsky.social below:

New TYP lab publication led by PhD student @alexlish.bsky.social out today in @cp-cellreports.bsky.social! We present a reproducible neuron-astrocyte-microglia tri-culture model to better reproduce intercellular interactions in Alzheimer's disease. Read more here: www.cell.com/cell-reports...

Amazing team science effort, led by Marius Wernig’s group, to develop a silencing-resistant transgene cassette that maintains expression following iPSC differentiation. This was major barrier for iPSC-based experiments. Thanks to CZI for funding the research! www.biorxiv.org/content/10.1...

Silencing of transgenes during iPSC differentiation is a frequent problem. 🧬🫢 Check out the piggyBac system below, which has resisted silencing in many protocols across many labs. 💪 Excited that our lab could contribute to this team effort led by @tuenaka.bsky.social & Marius Wernig!

Losing sleep over #iPSC transgene silencing after differentiation? Same here — too many times😭 So we built a PiggyBac construct that resists silencing. Expression stays ON, even after differentiation. Our #preprint is out👇 🔗 www.biorxiv.org/content/10.1... Supported by @cziscience.bsky.social