Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render) @zeiss-microscopy.bsky.social

We also tried it recently and indeed, it blinks nicely without any smelly buffers or extra stuff added. Makes SMLM sample preparation a breeze. I hope soon new additional colors will be discovered, apart from the far red.

🎄 A glimpse into #NanoTag Christmas dinner - Glühwein outdoors, a shared dinner, and Schrottwichteln 🎁♻️ where funny, used items are exchanged and find a new home. The past year has been special for us as we celebrate 10 years. Grateful for our team, customers, and partners. Here’s to what’s next 🥂

✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

Happy to tell that I got my tenured professorship at the University Medical Center in Göttingen 🇩🇪! Grateful for my students, mentors, international collaborators, the @nanotag.bsky.social team, and my family (dog included 🐾). Motivated to keep pushing science, innovation, startups, and education 🌍✨

#NanoTag joins the Life Science Ecosystem at Nordic Life Science Days #NLSDays2025 in Gothenburg!🇸🇪 Meet our CEO Hansjörg Götzke at the Booth B03:46 (German Pavilion), and discover our sdAb platforms🦙, protein production services, and research tools🔬advancing #LifeScience & #Biomedicine.

Fantastic talk of @ewerslab.bsky.social with ExM data on Septins and Unroof Cells in #GEF25!

🦙 NanoTag at #SingleDomainAntibodies Conference 2025! Our CEO Hansjörg Götzke will be in Utrecht sharing how our immune & synthetic sdAb platforms drive advances in research, diagnostics & therapeutics. Our founder Felipe Opazo @opazo.bsky.social will also be there to connect & share insights.

#ExM turned 10 this year - just like #NanoTag! 🎉 Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging. See you in Göttingen! #GEF25

Prefusion gB of HSV bound with a neutralizing nanobody www.nature.com/articles/s41...

🔬Wrapping up #SMLMS2025 @smlm-symposium.bsky.social in Bonn! Thanks to everyone who stopped by our booth! It was great connecting and sharing how our #sdAb-based solutions enhance precision, simplify #Multiplexing, and streamline workflows in #SuperResolution microscopy. See you next year! 🦙

Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism. www.nature.com/articles/s41...

🧬 New paper out! We explored why #antibodies have kept their 2 binding sites. Turns out, this “divalence” is crucial for B-cell receptors to sense antigen clustering and trigger immune responses, especially for weak or low-density antigens. Check full text: doi.org/10.1038/s414...

- A postdoc to work on cancer extravasation (4-year project). - A postdoc help in completing Filopodia-related lab projects (2-year). - Multiple Marie Curie postdoc positions that will open in the fall. - We will open a 5-year position to cover my teaching (70% research and 30% teaching).

🌞🦙 Summer makeover? You bet. Our alpacas ditched the fleece to stay cool – maybe your antibodies should too. sdAbs are high-performance tools made of just the antigen-binding domain (∼15 kDa, 3 nm). 🔬🧪Perfect for IF, super-resolution microscopy, IP & more. Browse our #nanobodies: nano-tag.com/shop/

💬Thank you, Dr. Maja Klevanski @majaklevanski.bsky.social (University of Heidelberg), for the great feedback on our recombinant anti-SARS-CoV-2 S1 antibody (rHcAb)! 😊 🔬 Applied in #dSTORM to study virion–cell interactions under antiviral conditions. Learn more about sdAb–Fc fusions 👉 bit.ly/3xdhYsn

🤹‍♀️ Still juggling primary antibodies from different species? Still stuck in sequential staining? ⏱️Switch to Smart Secondaries®🦙: ✅OneStep-IF ✅Same-species #multiplexing ✅Higher precision & reproducibility! Choose smart 👉 bit.ly/3UZxRMI #SecondaryNanobody #Microscopy #Immunofluorescence

@abberior.rocks — and NanoTag Biotechnologies rocks with them! 🤘 Smart Secondaries® 🦙now conjugated to abberior STAR dyes 🌈. Discover brighter, sharper, #STED -ready, species-independent #multiplexing & one-step IF. 🔬🤩 #SecondaryNanobodies #SuperResolution #FluorescenceMicroscopy

Preprint: IntAct-U-ExM enables robust, isoform-specific expansion microscopy of actin networks in yeast and mammalian cells. A cool study led by @anubhavdhar.bsky.social in collaboration with Sudarshan and Deepak Nair. www.biorxiv.org/content/10.1...

A new study by @jungmannlab.bsky.social presents #SPINNA, a model-based tool for quantifying protein stoichiometry, oligomerization state, and spatial arrangement from super-resolution data.🔬For precise labeling of #GFP & #mCherry fusion proteins researchers used our #nanobodies 😊 📄 bit.ly/4jTHfes

Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!

Fun fact: Alpacas love dust baths! 🦙💨 Spring at our farm means blossoms, sun & lots of happy rolling in the sand 🌸☀️ Just like our alpacas enjoy a refreshing dust bath, our clients enjoy smooth #sdAb discovery. Let’s roll into new discoveries together 🙃! #NanoTag #VHH #nanobody #AlpacaAntibodies

Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩 We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨

🚀 New: sdAb anti-Human #IgG! Expanding our #Smart Secondaries® (secondary #nanobodies) range with high-specificity sdAb clones for precise Human IgG detection. Minimized linkage error, superior penetration, ideal for one-step IF, ELISA, WB & therapeutic Ab testing. 👉 bit.ly/4ia1H9B

Finally a role for the most common synaptic marker in the world synaptophysin - maintaining synaptic vesicle diameter: www.science.org/doi/10.1126/...

Excited for our latest work! We developed nanobinders for the luminal domain of Synaptotagmin 1 (calcium sensor for synaptic vesicles). Thanks among others to @verstrekenlab.bsky.social @opazo.bsky.social @meunierlab.bsky.social @volkerhaucke-lab.bsky.social www.biorxiv.org/content/10.1...

Our lab at the NIH (Bethesda, MD) is looking for postdoctoral fellows to join our team studying the molecular mechanisms of protein trafficking and their links to neurodevelopmental disorders starting on or after 10-1-2025. Send your application to [email protected]. Please share and repost!

I am very excited about the invitation of @petrikursula.bsky.social to give a seminar at the Uni Bergen (UiB) 🇳🇴. I’ll share some of our work using @nanotag.bsky.social engineered #nanobodies with erasable signals for multiplexing microscopy. Paper: lnkd.in/een2yCDx Seminar: lnkd.in/esNGXhiv

We had a blast hosting curious young minds at NanoTag Biotechnologies for #Zukunftstag2025! From alpacas to #nanobodies, pipetting, microscopy, and liquid nitrogen fun❄️ 🌹with team from myriamed — science, teamwork, and discovery were in the spotlight! 🔬🧪🦙 #FutureDay2025 #ScienceIsFun #BiotechCareers

Interesting talks on the Extra Celular Matrix (ECM) session at the German Neuroscience Meeting here in #Göttingen #NWG2025; @neurowissg.bsky.social. Let's see what else is there interesting!

An interesting preprint from the Horrocks' lab detailing the behavior of secondary nanobodies (such a @nanotag.bsky.social ones) at the single molecule level and optimizing protocols for multiplexed labeling: doi.org/10.1101/2025...

I am Absolutely delighted to share the preprint of our last collaborative efforts where we investigated the impact of sonostimulation of Mesenchymal Stem Cells. Thread -1- www.biorxiv.org/content/10.1...

#FluorescenceFriday News! Super-resolution imaging gets a serious glow-up! ✨🌈 @nanotag.bsky.social & @abberior.rocks are bringing together the best of single-domain antibodies and cutting-edge fluorophores. Get ready to see your samples in a whole new light! 🦙+ 🍩 = #Nanobody + #STED

🔬 Two Göttingen innovators, @nanotag.bsky.social & abberior, team up to revolutionize super-resolution imaging! ✨🌈 By combining single-domain antibodies and advanced fluorophores, they’re set to transform how we see samples. 🍩🦙 #FluorescenceFriday #ComingSoon #Collaboration #Nanobody #STED

Explaining cutting-edge #immunofluorescence (IF) in 5 minutes? Challenge accepted!⏳😅 Join Dr. Steffen Frey at #TiM2025 as he pitches our #SmartSecondaries® properties, secondary #sdAb redefining #IF. nano-tag.com/technology/s... Mar 19, 2025 |14:50 |Münsingen #Microscopy #Nanobodies #Multiplexing

I am super happy to share our latest article entitled "Decoding the molecular interplay of CD20 and therapeutic antibodies with fast volumetric nanoscopy", now published in Science www.science.org/doi/10.1126/... It was my postdoctoral work mit @sauer-lab.bsky.social Congrats to all the co-authors!

Super happy to see the manuscript of my former PhD student and close friend Arindam Ghosh, @arindam92.bsky.social, and the Markus Sauer lab, @sauer-lab.bsky.social, being pblished in Science: www.science.org/doi/10.1126/...

✨ 𝗕𝗹𝗶𝗻𝗸, 𝗕𝗹𝗶𝗻𝗸! #FluorescenceFriday is perfect to launch new 🦙sdAb: 𝐅𝐥𝐮𝐨𝐓𝐚𝐠𝐬® 𝐜𝐨𝐧𝐣𝐮𝐠𝐚𝐭𝐞𝐝 𝐭𝐨 𝐉𝐚𝐧𝐞𝐥𝐢𝐚 𝐅𝐥𝐮𝐨𝐫® 𝟔𝟑𝟓𝐛 a new buffer-independent self-blinking dye. Making #SMLM more precise and reproducible! Now you find FluoTag®-X2 𝐚𝐧𝐭𝐢-𝐀𝐋𝐅𝐀, 𝐚𝐧𝐭𝐢-𝐆𝐅𝐏, and 𝐚𝐧𝐭𝐢-𝐦𝐄𝐨𝐬, more coming soon. nano-tag.com/shop/

...and there we have it - the first paper for 2025 😀 onlinelibrary.wiley.com/doi/10.1111/...

Great collaborative effort with good friends including @opazo.bsky.social @myelincentric.bsky.social @sigurbjornm.bsky.social and many others (sorry if I did not find the rest you on this platform yet).

Big congrats!! It’s a really great nanobody

My 1st post in bsky is about our latest paper of 2024. Excellent collaboration with @petrikursula.bsky.social @myelincentric.bsky.social & Bradley Zuchero labs. We characterized a set of 🦙 #nanobodies binding myelin protein CNPase. It is online at @jneurochem.bsky.social: dx.doi.org/10.1111/jnc....

Please share, we have #PhD opportunities for anyone interested in filming the #Biophysics of life at the nanoscale. Full details at: heath-afm-lab.github.io/positions/ Projects based around developing and applying High-Speed Atomic Force Microscopy methods to understand molecular dynamics.

The #FOMISUR workshop has started! Joined the gang including @lumasullo.bsky.social for a local beer

The big one is out! So over the moon to see our latest work with Stéphane Vassilopoulos published in Science. Check out how we reveal the unique architecture of clathrin-coated pits and endocytosis at the axon initial segment: www.science.org/doi/10.1126/...

Here's a starter pack that focuses on engineers/builders/makers/enablers of optical tools for (cell) biology. I'm pretty sure that the pack is incomplete - if you want to join the list, please reply with your favorite probe (that you made or used) and I'll add you 🤓 go.bsky.app/2YVvYER