How to determine the proteome of cellular compartments? We established a method to do just that, published n @eLife.bsky.social: SPEx: Compartment-Resolved Proteomics via Expansion Microscopy–Guided Microdissection doi.org/10.7554/eLif...

Saishree S. Iyer and Anna Akhmanova @utrechtuniversity.bsky.social review the mechanisms controlling plus-end elongation of centriolar and ciliary #microtubules, revealing shared principles. rupress.org/jcb/article/... #Centrioles #Cilia #Cytoskeleton

Chouhan, Phogat et al. of the Mahak Sharma lab report that the small GTP-binding protein Arl8b recruits the Rab11a GAP, TBC1D9B, to inactivate Rab11a-mediated recycling of newly synthesized LAMP1 and mediate its efficient sorting to #lysosomes. rupress.org/jcb/article/...

Acute lysosome repositioning reveals functional and proteomic adaptations https://www.biorxiv.org/content/10.64898/2026.05.17.724935v1

We wrote a short review article on the benefits, challenges and future prospects of studying protein trafficking with proximity labeling revolving recent technical advances. www.sciencedirect.com/science/arti...

SPEx: Compartment-Resolved Proteomics via Expansion Microscopy-Guided Microdissection https://www.biorxiv.org/content/10.64898/2026.03.28.714993v1

Now online! Cell-type resolved protein atlas of brain lysosomes identifies SLC45A1-associated disease as a lysosomal disorder

Thrilled to share our latest study, led by @reikatei.bsky.social, in @natchembio.nature.com! We began by asking a simple question—how do cells know if they have too much of a lipid in a particular membrane, and how do they respond to rectify this imbalance? www.nature.com/articles/s41... More info 👇

A super detailed protocol + video on Cryo-ExM - Cryo-Expansion Microscopy, led by the labs of our former postdocs @marinelap.bsky.social & @ebertiaux.bsky.social. Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...

Latest preprint from our lab reports that the distinct pH of anterograde (less acidic) and retrograde (more acidic) lysosomal vesicles in the axon depends on assembly of the V1 and V0 domains of the vacuolar H+ ATPase, mediated by the metazoan RAVE complex www.biorxiv.org/content/10.6...

🚀 Our new paper is out @natmethods.nature.com! Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells. 🔗 www.nature.com/articles/s41... 🧬 www.addgene.org/John_Ngo/

The axonal ER couples translation and secretion machineries for local delivery of axonal transmembrane proteins to promote axonal development www.biorxiv.org/content/10.1...

Wow, this paper provides crucial understanding of local translation and processing of transmembrane proteins in axons, one of the big mysteries that have haunted the field for a long time!!!!!!!! Fantastic!!!!!!!! Nguyen et al., 2025, bioRxiv 2025.09.09.674816ff. -- doi.org/10.1101/2025...

Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.1.... We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.

The involvement of the ER-phagy receptor FAM134B in membrane contact sites between ER and endolysosomes promotes ERLAD https://www.biorxiv.org/content/10.1101/2025.07.08.663682v1

The first review paper from our lab is out!! @viviennebauer.bsky.social discussed the rapidly emerging knowledge on (the interplay between multiple) organelles and neuronal mRNA localization. Hope you enjoy the read! www.sciencedirect.com/science/arti...

www.nature.com/articles/s41... POTATOMap is officially out today! (Even tho it says 2024Dec30… yeah long story😂) Thank you to all the authors and everyone involved! I hope we have provided something useful and maybe leave a little (just a little) impact in your research.