Congrats to my friends in the Boettiger lab for this really beautiful live imaging work. A big leap forward in understanding the dynamic side of genome organization. www.science.org/doi/10.1126/...

Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1... Read the thread below for a summary of our findings 🧵1/11

EpiSci - Hi there! Our review “Mechanotransduction in stem cells” is finally published. With @carstenschulte4.bsky.social we provided a comprehensive picture, that aims to cover the whole mechanotransductive pathway from the cell microenvironment to the nucleus. 1/3 pubmed.ncbi.nlm.nih.gov/38729084/

And we are done --- #SMLMS2025 is over, and it was great. Thank you Uli & Ulrike & all the helpers 👍, and thanks to everyone joining this event 🙏 ... safe travels home & see you soon!

Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism. www.nature.com/articles/s41...

Excited that our paper was published in Science Advances 🧪 Great work by first author Merrick Pierson Smela who will carry this work forward in his new start-up!

Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here: meetings.embo.org/event/26-in-...

🚨New preprint from the lab🚨 🧬 What keeps certain chromatin domains anchored at the nuclear periphery? Our new genome-wide HiDRO screen uncovers a key role for RNA-binding protein hnRNPK. www.biorxiv.org/content/10.1...

(1/n) DNA-PAINT imaging inside the nucleus at single antibody resolution using TIRF? Ultrathin sectioning makes it happen! Grateful to share my postdoctoral work introducing “tomographic & kinetically-enhanced DNA-PAINT” or in brief: tkPAINT. Out in @pnas.org! doi.org/10.1073/pnas... 👇🧵

We’re excited to share LiteLoc — a lightweight and scalable deep learning framework for high-throughput single-molecule localization microscopy, enabling analysis speed of >500 MB/s on 8× RTX 4090 GPUs without compromising accuracy. rdcu.be/eztp6

🚨 2 × PhD positions @EPFL! 🚨 Help us push the boundaries of fluorescence microscopy - DNA nanotech, custom optics & spatial omics in Lausanne 🇨🇭. Start Jan 2026. Send CV + motivation + 2 refs → [email protected] #PhD #Hiring #microscopy #SuperResolution #SpatialOmics #DNAPAINT #FLASHPAINT

Many proteins bind RNA, yet we still don’t know what RNAs most bind because methods map one RBP at a time. In @cp-cell.bsky.social, with the Jovanovic lab, we describe SPIDR – a method for mapping the RNA binding sites of dozens of RBPs in a single experiment. www.sciencedirect.com/science/arti...

What allows lamins A and C to confer much stronger mechanical stability to the nuclei than lamin B? We show that even though all lamins can protect the nucleus from small cell intrinsic forces, only lamin A/C protect the nucleus from large deformations: www.biorxiv.org/content/10.1... 1/n

Another paper bluetorial! Today: how does the spatial location of genes influence their function? (1/n) www.nature.com/articles/s41...

EpiSci - 🧬 - 🧪 - The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate www.nature.com/articles/s41...

(1/n) A simple, ultrastable, and cost-effective oxygen-scavenging system for long-term DNA-PAINT imaging Please check out our new study on the benefits of sodium sulfite as oxygen scavenging system for DNA-PAINT. 👇🧵 #SuperResolution #DNAPAINT #SMLM #Microscopy

This seems like a really important paper comparing chromosome tracing methods, demonstrating that classical denaturing DNA-FISH and chromosome tracing leads to substantial distortion of 3D genome structure, likely due to the high-temperatures involved (70-90C): www.nature.com/articles/s41...

The review on live-cell SMT that I contribute to the jmolbiol.bsky.social special issue ‚Imaging of the central dogma‘ is now online as pre-proof: www.sciencedirect.com/science/arti...

Calling all aspiring Postdocs! One extra week to apply to join our exciting HFSP-funded project to uncover how chromatin moves to function. Deadline July 15th. www.mdc-berlin.de/career/jobs/...

⚠️ I am really excited to share the work of Anastasios Balaskas, an excellent PhD candidate in the lab, with the wider world. Tasos made a significant advance: generating a stem cell-based embryo model that contains both posterior and anterior neural tissues of the late-stage gastrulating embryo.

We wrote a review on Transposable Elements (TEs) and almost all aspects of TE silencing and their roles in biological processes & disease. www.nature.com/articles/s41...

How to represent genomes in 3D? In this paper (and video), we propose Geometric Diagrams of Genomes (GDG)—a visual grammar that aims at emulating the effect ribbon diagrams (Richardson et al 1981) had in protein structural biology. Time will tell.😅 👉🏼 genomebiology.biomedcentral.com/articles/10....

The 3rd Berlin Single Molecule Biophysics Course will run from 8th to 12th September at the HU-Berlin, Institute of Biology. Learn about single channels, single molecule TIRF, theory and analysis. Apply by 7th July at [email protected]. Fee waivers and bursaries available. ! Please share widely !

🚨 New preprint! Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM www.biorxiv.org/content/10.1...

This is a piece that I and @karsten-rippe.bsky.social discussing a lot, and a topic that is very close to my heart. The editors @naturerevgenet.bsky.social gave us the stage to do so, and the final version of our review is now available under this link: rdcu.be/erP1u A short thread follows 1/n

Spotiflow, our deep learning based spot detection method for microscopy, is now published in @natmethods.nature.com! Since the pre-print, we have added many features, notably native 3D detection! @maweigert.bsky.social @gioelelamanno.bsky.social @epfl-brainmind.bsky.social Paper: rdcu.be/epIB7 (1/N)

Great new DNA/Q-Paint method published by our @ucl colleague Sabrina Simoncelli - lucky to have been part of it! Check out the Ecadherin and collagenIV imaging in the fly retina at up to 15micron depth www.nature.com/articles/s41...

@science.org Expansion in situ genome sequencing links nuclear abnormalities to aberrant chromatin regulation | Science www.science.org/doi/10.1126/... @jbuenrostro.bsky.social @eboyden3.bsky.social + al @broadinstitute.org @harvard.edu #ExIGS #Chromatin #Lamin #Progeria #Aging #ExpansionMicroscopy

Applications are now open for the 4th edition of the “Ecole de Physique des Houches” on Fluorescence Markers for Advanced Microscopies, a WE-Heraeus seminar. March 15th-20th 2026 Join us to learn everything you wanted to know about Fluorescent Markers for advanced microscopies.

Excited our paper is out in Cell @cp-cell.bsky.social! 🧬⚡ DNA photo-crosslinking proteomics in living cells 🎯 Pinpoints protein-DNA interactions to single amino acids 🌎 Globally quantifies DNA binding for >1800 proteins at a timescale of minutes 🔗 www.cell.com/cell/fulltex... 🧵

New paper "Chromatin unfolding via loops can drive clustered transposon insertion" in @biophysj.bsky.social Former postdoc Roshan Prizak & predoc Aaron Gadzekpo find that a feedback between transposon insertion and chromatin accessibility can give clustering. www.cell.com/biophysj/ful...

(1/n) Thread @matteomazzocca.bsky.social @domenicnarducci.bsky.social Simon Grosse-Holz @jessematthias.bsky.social preprint Q: how does chromatin move? Using MINFLUX, SPT & SRLCI, we track chromatin dynamics across 7 orders of magnitude in time to provide answers www.biorxiv.org/content/10.1...

1/ Super excited to share my first preprint from @andersshansen.bsky.social Lab! We used MINFLUX to track chromatin (H2B-Halo and Fbn2 locus) at an unprecedented 200 μs, then combined it with SPT to span μs-minutes (H2B) or SPT & Super-Res Live-Cell Imaging (SRLCI) to span μs-hours (Fbn2)

New paper out! Would you like to fluorescently label the plasma membrane in live, fixed or permeabilized cells? I tested different lipid structures and labelling approaches to decide what makes a good membrane labelling probe for such imaging experiments! www.biorxiv.org/content/10.1...

Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩 We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨

The awesome Helene Kretzmer and I are looking to recruit a join computational PhD student. Applications will be evaluated on a rolling basis, so don't wait! Any questions get in touch. We are looking forward.

New lab preprint! Unveiling the Molecular Architecture of T Cells and Immune Synapses with Cryo-Expansion Microscopy (Cryo-ExM). A fantastic collaboration with Dr. Benita Wolf. Congratulations to all authors, especially Florent Lemaitre, for leading this amazing work! www.biorxiv.org/content/10.1...

Happy to see my Postdoc work out @natbiotech.nature.com. Using HaloTag and synthetic fluorophores instead of FPs enables us to shift the spectral properties of kinase activity reporters (KARs) to the far-red 🔬. Huge thanks to everyone involved @jinzhanglab.bsky.social! www.nature.com/articles/s41...

Great postdoc opportunity in the Farabella lab: leverage your computational skill set to study how non-coding elements affect gene regulation & 3D genome organization 💻🧬🐟💥🔬!

Our new findings on how chromosomes get ready for cell division are now published in @cellpress.bsky.social! Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩 www.sciencedirect.com/science/arti...

I’m excited to share our latest research from the Musser Lab, published today in Nature. Using two-color 3D MINFLUX, we directly visualized nuclear import and export events through transport-active nuclear pores. (1/5) www.nature.com/articles/s41...

Are you interested in deciphering chromatin structure using microscopy? 🔬 👇👇The Ellenberg lab is hiring! 👇👇 📣 Please share 📣

Excited to share our lab’s first review, focusing on RNA localization! #RNA #RNAlocalization genomebiology.biomedcentral.com/articles/10....

By putting a donut between our faces and each article we read, we were able to extract more information than ever before about MINFLUX. You can read the new review article from @maxperutzlabs.bsky.social by Lukas Scheiderer, myself, and @jonasries.bsky.social at www.nature.com/articles/s41...

I am hiring: PhD students, postdocs, admin personnel. Email me if you know somebody that may be interested in joining us in Cologne! 👇👇 Please RT for reach! 🙏🙏

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?

If you're interested in Zappone's amazing work, check out these key references: 🔗 www.nature.com/articles/s41... 🔗 doi.org/10.1101/2024... No doubt #SPADarray detectors will change fluorescent fluctuation spectroscopy. Seminal work by Zappone, Perego, and @elislenders.bsky.social

New paper out 🎇 It has been 13 years since @owenlabuob.bsky.social and co. published a stellar protocol on how to measure membrane order with smart probes! Since then, there has been new dyes, imaging modes and biology! We wrote a protocol for Nature Protocols: www.nature.com/articles/s41...

My German election ballot only arrived today, a long shot but is anyone traveling from Boston to Berlin today who could take it? Any leads @germanyinboston.bsky.social ?

New preprint from the @gerlichlab.bsky.social @imbavienna.bsky.social! How do DNA breaks locate homology sites in the vast space of the human genome? We show how cohesin guides homology search for faithful repair! Read more 👉 https://doi.org/10.1101/2025.02.10 Follow along for key insights! 🧵