1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵 🔗 www.nature.com/articles/s41... #CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics

Please check out our newest work on fluorogenic speed imager probes, in which we combine the strengths of speed-optimized imagers and fluorogenic imagers. #SuperResolution #DNAPAINT #microscopy www.biorxiv.org/content/10.6...

DNA-PAINT with photocaged DNA labels = PhotoPAINT🔬. Why? No need for washing or mixing 👉🏻 higher accuracy in DNA-PAINT & other super-res methods. Fantastic collab with Alex Heckel & driven by the 'magic team' Nina Kaltenschnee and Marina Dietz 💪 doi.org/10.1002/anie... @goetheuni.bsky.social

β-barrel nanopores designed for insertion into thick block copolymer membranes https://www.biorxiv.org/content/10.64898/2026.03.13.711555v1

Neat paper from Schwille lab. They demonstrate constriction of synthetic vesicles using important components of the bacterial cell division machinery, FtsZ and FtsN. They induce strong constriction of Z-rings in vesicles just by adding the cytoplasmic domain of FtsN www.nature.com/articles/s41...

New paper alert! Authors introduce 𝗚𝟱𝗠, 𝗮 𝗗𝗡𝗔-𝗣𝗔𝗜𝗡𝗧-𝗼𝗽𝘁𝗶𝗺𝗶𝘇𝗲𝗱 𝗚𝗮𝘂𝘀𝘀𝗶𝗮𝗻 𝗠𝗶𝘅𝘁𝘂𝗿𝗲 𝗠𝗼𝗱𝗲𝗹𝗶𝗻𝗴 𝗮𝗹𝗴𝗼𝗿𝗶𝘁𝗵𝗺 that accurately determines true molecular position, even at the 𝗥𝗮𝘆𝗹𝗲𝗶𝗴𝗵 𝗹𝗶𝗺𝗶𝘁, and 𝗶𝗺𝗽𝗿𝗼𝘃𝗲𝘀 𝗲𝗳𝗳𝗲𝗰𝘁𝗶𝘃𝗲 𝗿𝗲𝘀𝗼𝗹𝘂𝘁𝗶𝗼𝗻 𝗯𝘆 ~𝟳𝟬%. Huge congratulations to all authors! www.nature.com/articles/s41...

We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells. www.nature.com/articles/s41... rdcu.be/eQVxt

I was invited by @focalplane.bsky.social to write a short description about it: focalplane.biologists.com/2025/12/12/a...

A Cinderella story: raising CLIP-tag from the ashes to shine in fluorescence labeling. www.biorxiv.org/content/10.6...

#HappyMicroscopyMonday #microscopycommunity-Join the AIMM user community on Friday, March 6, for a talk by Hannah Somers- “Stop Reinventing the Protocol: MicroHub, a Searchable Hub for Microscopy Knowledge” Learn more: buff.ly/kZSIkSJ

Final version @nature.com of our paper describing unconventional multicellular development in a choanoflagellate inhabiting an extreme environment. A ton of new data since the first @biorxivpreprint.bsky.social preprint (which we've kept updating). A brief 🧵 (carried over from the old place)

🙏 This study was powered by fantastic collaborations — especially with @abelljonny.bsky.social & @joechambers.bsky.social with contribution form @felixmendu.bsky.social @lasergroup.bsky.social @franbottanelli.bsky.social 🔬🧠 www.nature.com/articles/s41...

Continuous mass photometry by single molecule trapping https://www.biorxiv.org/content/10.64898/2026.02.02.703204v1

Ragaller, F., Sjule, E., Urem, Y. B., ..., Blom, H., & Sezgin, E. (2024). Quantifying Fluorescence Lifetime Responsiveness of Environment-Sensitive Probes for Membrane Fluidity Measurements. The journal of physical chemistry. B, 128(9), 2154–2167. #EpithelialMechanics buff.ly/YjCWSfP

A protein called Uninflatable acts as a switch between glial cells growing and glial cells wrapping themselves around axons. 🔗 buff.ly/WRdybO3

Great news! @mariaingaramo.bsky.social 's company (Nonfiction Labs) made a remote-controlled antibody. Its binding turns on and off with a magnet. This is a HUGE step towards our dream of magnetically controlled drugs. Imagine a cancer drug that ONLY attacks the tumor, not the rest of your body.

New paper from @zhixingchen2.bsky.social's lab! It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime! www.nature.com/articles/s41...

New paper out! Combining single-objective light-sheet microscopy and time-resolved SPAD array detection, we massively accelerate fluorescence lifetime imaging (FLIM) compared to confocal FLIM, making FLIM applicable to 3D specimen such as organoids and embryos. www.nature.com/articles/s42...

The new version of TrackMate, the v8.1 has just been released. It builds upon the recent v8, linked below, and offers two main improvements 👇

📢 Paper alert 📢 Chirality is known to be important for the movement of microorganisms and active matter. In our new paper out today in @natphys.nature.com, we show that chirality is used by malaria parasites to control their motion patterns: doi.org/10.1038/s415... Here comes a 🧵 ... (1/9)

Our new paper is published in the Journal of Chemical Biology! 🏮 We share StayRose, a red version of StayGold 🏮 StayRose has an unnatural amino acid-based chromophore, and has the same immense photobleach-resistance as StayGold 💡

Just published in Nat. Neurosci. Led by J.P. Ferrari-Souza, this study used imaging & fluid biomarkers to reveal that microglia modulate Aβ-related astrocyte reactivity, which in turn contributes to AD progression. @erzimmer.bsky.social @natureportfolio.nature.com www.nature.com/articles/s41...

I did not expect that: Large Language Models are invertible: arxiv.org/abs/2510.15511

Daniel Abbühl, Philippe Bastin @bastinlab.bsky.social and colleagues use a novel approach for tagging tubulin to dissect microtubule assembly dynamics of the axoneme in Trypanosoma brucei. #JCSciliaSI journals.biologists.com/jcs/article/...

PhD project available in my lab. Email me with CV if interested www.findaphd.com/phds/project... #microsky #bioimaging

Neat - 'bubble-driven cell detachment' from Kripa Varanasi and co. doi.org/10.1126/scia...

Intéressant, ça : les critères de sélection du prochain Scientifique en chef du Québec ont été publiés cette semaine. Des volontaires ? 😉 publicationsduquebec.gouv.qc.ca/fileadmin/ga...

I never thought watching microtubules break could be so interesting! I learned so much about mechanics and modeling in this work and am extremely grateful to Archie Geng for leading the way. Also huge thanks to François Nédélec for creating Cytosim and providing advice along the way.

"Large-scale visualization of α-synuclein oligomers in Parkinson’s disease brain tissue," NatBME doi.org/10.1038/s41551-025-01496-4 A culmination of wild brainstorms, imaging marathons, analysis breakthroughs and setbacks, around the clock work before conferences, and new friends along the way! 🧠🎆

The culmination of many years work. One of the most advanced light-sheet microscopes in the world! -> A large field of view Snouty light-sheet microscope, with any immersion remote refocus and real-time multi-angle projections! For details see previous posts and here: github.com/amsikking/HT...

RFdiffusion3 is here! We train a general network that explicitly models every atom and use it to design active enzymes and DNA binders. Tremendous team effort with Jasper, Rohith, Raktim, Rafi, Yanjing, Paul, Jonathan, and many others! Check it out: lnkd.in/eiUFfJaM.

KIF13B controls ciliary protein content by promoting endocytic retrieval and suppressing release of large extracellular vesicles from cilia: Current Biology www.cell.com/current-biol...

Examining measures of executive function & MRI brain data from 497 252 UK Biobank cases, researchers find that pre-diagnosis MRI grey matter volume was lower in #Parkinsons (vs controls), in addition to curious trends in other disease indications academic.oup.com/braincomms/a...

New article online: Ångström-tunable polarization-resolved solid-state photon sources. go.nature.com/4gdVWYR

Reconstructing long-range axons from dense brain images is tough. This study introduces a novel method that separates axon identification from global statistical rules, showing big improvements over existing tools for mapping neuronal projections. buff.ly/OBKr9cM

New article online: Extreme-ultraviolet spatiotemporal vortices via high harmonic generation. go.nature.com/4m0Tc2e

Excited to share my postdoc work from the Eggeling lab in Jena (@leibnizipht.bsky.social, #FSUJena, #KTHuniversity) on bringing smart microscopy to super-resolution MINFLUX: event-triggered MINFLUX microscopy.

YO, what's up with all these almost-witchcraft techniques?? In this paper, they develop a way to freeze cells so quickly that it preserves Calcium waves midway!! WHAT??? HELLO??? So now you can do 3D reconstruction of calcium waves with just a normal confocal. www.nature.com/articles/s41...

🧬🔬🧪 🎉 Our team at @czbiohub is thrilled to share TWO companion papers out today in @NatureMethods! 📦 Ultrack — robust, scalable nD cell tracking 🌐 inTRACKtive — a beautiful, open-source web viewer for lineage exploration Let’s dive in! 👇 www.nature.com/articles/s41... www.nature.com/articles/s41...

There is still time to apply to become part of our team at Nature Biomedical Engineering! Please share! springernature.wd3.myworkdayjobs.com/SpringerNatu...

Can we learn motion models from post-processed tracks? 🧐 Not really 😢 Emission noise accounts for ~99% of the likelihood. TLDR: What you think is anomalous diffusion… might just be noise. 🤷‍♂️🤷‍♂️🤷‍♂️🤷‍♂️ 🔗 Read more in our latest preprint: arxiv.org/abs/2507.05599 #Biophysics

It's out & hot 🔥 Not only because it just went online but since we show that engineered heat patterns are super effective in manipulating fluid flows & particles. If you work in microfluidics, or need reconfigurable environments in your experiments, give it a read! www.nature.com/articles/s41... 🧪💡

New open access article online: Microscale generation and control of nanosecond light by light in a liquid crystal. go.nature.com/3H0mT5a

Super-resolution live-cell mapping of protein-protein interactions using chemogenetic split reporters and STED microscopy https://www.biorxiv.org/content/10.1101/2025.08.05.668682v1

#PALH: How to convert a #3Dprinter to a personal #automated #OpenSource #liquid #handler for life #science workflows: - based on #Ender3 - cost: US $400 - #PCR setup, #DNA purification using #magnetic beads doi.org/10.1016/j.sl... #DIYbio #DIYchem #chemistry #lab #instruments #pipet #automation

Wyss scientists have invented a novel metabolic labeling technology that enables the “tagging” of the surface of T cells with immune-enhancing cytokine molecules, which could help expand adoptive T cell therapies to the treatment of solid tumors.

Ångström-resolution imaging of cell-surface glycans Now published in Nature Nanotechnology! 🤩 @natnano.nature.com www.nature.com/articles/s41...

Pieces of the puzzle coming together: Researchers report #Parkinsons-associated GBA1 mutations impair Sonic Hedgehog signaling & Hedgehog-dependent neuroprotective factor production by a distinct mechanism www.pnas.org/doi/10.1073/...