#Archaeasky #Microsky #FtsZ

PhD project available in my lab. Email me with CV if interested www.findaphd.com/phds/project... #microsky #bioimaging

🚀🔬 Announcing #IMS2026 at @itqbnova.bsky.social! We're assembling a #LifeSciences #Microscopy symposium packed with ✨ speakers and 🧑‍🔬 workshops. All about imaging cells with photons, electrons and AI! If you love microscopy, you need to be here. Join us on March 19! ims2026.itqbnovacommunity.org

Letting AI anywhere near my writing is a big line in the sand for me. No thank you.

C̳H̳E̳C̳K̳ ̳I̳T̳ ̳O̳U̳T̳ Spectacular atomic force microscopy time course studies from Carolina Borrelli et al. in Nature Microbiology show how polymyxin drugs (like colistin) cause blebbing and shedding of the Gram-negative bacterial outer membrane It looks almost like the bacilli 🦠 caught smallpox then died

Thrilled to share that our article in @jofmicroscopy.bsky.social is now accessible online and as open access! If you wonder how to start the analysis of your images, we propose a decision tree to help identify suitable methods. Just follow the link(s)... #imageanalysis #bioimaging

BindCraft: an AlphaFold2-powered de novo protein binder design tool 🧬✨ Tested in some bacterial systems too! Designs so far are short peptides or mini-domains. Looking forward to long/full protein design ahead! 🧪 #microsky www.nature.com/articles/s41...

And the crispri genetic interaction screen 👏💫🤯 #microsky from J-W Veening lab. Dual CRISPRi-seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle: Cell Systems www.cell.com/cell-systems...

www.biorxiv.org/content/10.1... #microsky

Dual transposon sequencing profiles the genetic interaction landscape in bacteria | Science www.science.org/doi/10.1126/...

Really enjoying reading this primer on single particle tracking #microscopy by @svanteeffelen.bsky.social and colleagues www.nature.com/articles/s43... (pdf on Sven's website)

#MicroSky yeah, cable bacteria 🤗

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators. www.nature.com/articles/s41...

The sun cast incredible crespuscular rays during last night's saffron hued sunset. Bay Ridge, Brooklyn

you know the chaperones that 𝘌. 𝘤𝘰𝘭𝘪 has at its disposal, don' you? GroEL/GroES, DnaK/DnaJ/GrpE, Trigger Factor (TF), ClpB, Hsp33 and Spy... but do you have the ribosome on your checklist? you should! 👇 www.biorxiv.org/content/10.1... #CryoEM #CryoET #TeamTomo #MicroSky

Come and work with us in beautiful Bath.

🚨 New paper alert! 🚨 MinD is famously known for positioning FtsZ at mid-cell in bacteria In our study, myself and @iduggin.bsky.social found that MinD homologues in Haloferax volcanii influence the localisation of CetZ1, a homologue of FtsZ and tubulin #Archaeasky #Microsky

*15%* of all the biomass on Earth is buried deep in the crust. 70-90% of all bacterial (and archaeal) biomass is found at depths from a few meters to 10 km below the soil line. We can call these deep denizens: 'INTRATERRESTRIALS'. Let's talk about the Deep Biosphere. (🧑‍🎨: Verena Heuer, Marum)

Going to a conference and hope someone posts about your talk? Make it easy for the posters!! Tips: 1. Include your handle at beginning and end 2. Make clear what is published/not 3. Have a conclusions slide with key message 4. Highlight new preprint or publication if relevant #AcademicSky 🦠🧪🧬

They exist! Please tell the authors that when you review such papers 😊

this book everythingistb.com 🦠🧪 #Tuberculosis

VACANCY - We’re seeking an Optical Microscopy Support Specialist to join our Bioimaging Platform, to help train users on light microscopes, collaborate on imaging projects and provide technical support. www.jic.ac.uk/vacancies/op... Closing date - 11 August 2025 Contract - Full time, indefinite

#EUProject | #BioImaging & AI ➡️ BioImage Model Zoo by @ai4life.bsky.social is a platform for #AI models in biological imaging ➡️ To find out about the training courses and tools available to help you use the platform correctly for your area of study, visit: www.embrc.eu/project/ai4life/ 🌊🧪🦑

Very interesting paper on structural biology and biochemistry of Staph aureus FtsW:PBP1 (PBP1 is the divisome bPBP partner). Amongst other things, looks like some very interesting results on interaction of PBP1 PASTA domains with peptidoglycan pmc.ncbi.nlm.nih.gov/articles/PMC... #MicroSky

Nature Microbiology put together a good research briefing on this Rudner lab bacterial turgor regulation/ c-di-AMP paper. A supportive quote from my peer review comments is included. #microsky #bioimaging rdcu.be/euQln

Are you looking for a postdoc? I will be looking for 2-4 people after the summer to join the lab !! Do not hesitate to reach out and disseminate !!

Really cool paper on turgor regulation in Bacillus subtilis www.nature.com/articles/s41...

If you are an established scientist interested in moving the UK, the Royal Society Faraday Fellowships can support this. royalsociety.org/news/2025/05... If you are interested in moving to University of Warwick School of Life Sciences via this fellowship, get in touch! #microsky #microscopy

👉🏻 so glad to see this work out now ... a masterpiece conceived, led and driven by 👉🏻 Christoph Spahn 👏@michriscopy.bsky.social (🚨 #NewPI) and with great collaborations 🙏 @henriqueslab.bsky.social @seamusholden.bsky.social @gomez-mariscal.bsky.social

With the Bacterial Cell Biology and Development GRC coming up next week, thought I'd re-share this Microbial Cell Biology starter pack. Looking forward to seeing my people on Sunday!

Update on initial results of testing GraceBiolabs potential replacement to Thermo Fisher GeneFrames, widely used for bacterial cell biology microscopy time lapses but soon to be discontinued #microsky #bioimaging

Really cool preprint on the effects of membrane tension in bacterial cell division

yes, noah, this 𝗶𝘀 cool! #MicroSky afaik only 𝘉𝘰𝘳𝘳𝘦𝘭𝘪𝘢 keeps their flagella closer to their body: in the periplasm 😉

Can't miss this cool paper that came online while I was away! This set of rhodamine dyes is incredibly versatile for bioimaging. Check out the paper and see if want to try them with your HaloTag-labeled proteins!

we're glad to boost this! 👇 #MicroSky

Does anyone know how publishers designed books before computers? It came up last night in conversation and I realised I have no idea how layouts happened pre desktop publishing! #Publishing

How did I miss this paper? @ronanmccarthy.bsky.social lab screened nosocomial bugs for plastic-degrading enzymes, reasoning that they've been selected for this ability. They found a P. aeruginosa enzyme that efficiently degrades plastic #Biofilms11 #MicroSky (Image: www.cell.com/cell-reports...

🦠🦠🦠🦠🦠🦠🦠🦠🦠🦠 𝕌𝕟𝕚𝕧𝕖𝕣𝕤𝕒𝕝 𝕊𝕦𝕣𝕧𝕚𝕧𝕒𝕝 𝕊𝕨𝕚𝕥𝕔𝕙 🦠🦠🦠🦠🦠🦠🦠🦠🦠🦠 𝔹𝕒𝕔𝕚𝕝𝕝𝕦𝕤 𝕤𝕦𝕓𝕥𝕚𝕝𝕚𝕤 𝕡𝕖𝕣𝕤𝕚𝕤𝕥𝕖𝕣𝕤 𝕗𝕠𝕣𝕞 𝕥𝕙𝕣𝕠𝕦𝕘𝕙 𝕒 𝕔𝕠𝕞𝕞𝕠𝕟 𝕞𝕖𝕥𝕒𝕓𝕠𝕝𝕚𝕔 𝕤𝕨𝕚𝕥𝕔𝕙 𝔸𝕝𝕝 𝕡𝕒𝕥𝕙𝕤—𝕤𝕥𝕒𝕣𝕧𝕒𝕥𝕚𝕠𝕟, 𝕤𝕥𝕣𝕖𝕤𝕤, 𝕠𝕣 𝕒𝕟𝕥𝕚𝕓𝕚𝕠𝕥𝕚𝕔𝕤—𝕥𝕣𝕚𝕘𝕘𝕖𝕣 (𝕡)𝕡𝕡𝔾𝕡𝕡 𝕓𝕦𝕚𝕝𝕕𝕦𝕡, 𝕕𝕖𝕡𝕝𝕖𝕥𝕚𝕟𝕘 𝔾𝕋ℙ 𝕒𝕟𝕕 𝕗𝕝𝕚𝕡𝕡𝕚𝕟𝕘 𝕔𝕖𝕝𝕝𝕤 𝕚𝕟𝕥𝕠 𝕕𝕠𝕣𝕞𝕒𝕟𝕔𝕪 𝔽𝕣𝕠𝕞 𝕥𝕙𝕖 𝕁𝕦𝕖 𝕎𝕒𝕟𝕘 𝕃𝕒𝕓 (𝕌𝕎–𝕄𝕒𝕕𝕚𝕤𝕠𝕟) ℕ𝕒𝕥 𝕄𝕚𝕔𝕣𝕠𝕓𝕚𝕠𝕝

I forgot to post about this recent paper - exciting work led by @michriscopy.bsky.social showing clearest evidence yet for transertion strongly tethering the bacterial nucleoid to the membrane during fast growth. It was a pleasure to contribute a little 🔬🦠🧫 www.nature.com/articles/s41...

We ordered IS203 iSpacers for the test. I even communicated with the manufacturer (Sunjinlab) and they swiftly replied that custom modification of the size is possible! 💚 I suggest we all test iSpacers and pehaps convince Sunjinlab to make an affordable price for the new size (GeneFrames size)

We got today samples of iSpacer from SunJinLab. www.sunjinlab.com/ispacer-sele... It seems promising, although might be a bit too thin on the short side of the rectangle. I'll update once we've tested it properly.

Opportunity alert! It's also not in the USA.

I got hit by some rather sudden and extreme financial hardship so if anyone is in need of remote wetlab contract research, strictly BSL1, do let me know. Currently scrambling for gigs. Plant, Bacterial, Archaeal Non-model Bioeng Custom Lab Hardware Turn Key Genetic Design Please repost for reach 💚

An interesting #PlantSciJob as Editor at Nature Microbiology @natmicrobiol.nature.com. Expertise in #PlantMicrobiology #MicrobialEcology #PlantImmunity desirable. #PlantScience #PlantSciJobs #PlantScienceJobs

Two ERC-funded postdoc positions available in the team - one here and second to follow - please repost! jobs.ncl.ac.uk/job/Newcastl...

Fellow bacterial cell biologists, message me if you use @thermofishersci.bsky.social Gene Frames for microscopy! Thermo are planning to discontinue these in September but have indicated they will look into this further. If I make list of labs using them Thermo might not kill the product Please RT🔬🦠

Developments on the lipid II flippase debate in bacterial cell wall synthesis - evidence that the SEDS proteins may also be flippases journals.asm.org/doi/pdf/10.1... #MicroSky

Very Happy to finally see this story out. I remember the first time @archaeon-alex.bsky.social showed me the videos maybe 3-4y ago on a Zoom call ! Mind blown then and still mind blown today. A must read for all.