AMaNITA: an end-to-end workflow for native tRNA nanopore sequencing data analysis 💫🦠 www.biorxiv.org/content/10.6...

Anticodon loop remodeling and D-stem shape drive the specific recognition of ANN-decoding tRNAs for t6A modification 💫 🦠 academic.oup.com/nar/article/...

www.science.org/doi/10.1126/... 🦠💫💫💫

Epigenomes in thermophilic microbial communities and their impact on the interaction across prokaryotes and mobilomes | bioRxiv www.biorxiv.org/content/10.6...

We've been learning a lot about how Enterococcus faecalis interacts with neutrophils lately. In our latest preprint, @harisantypas.bsky.social describes how lactic acid from E. faecalis dampens neutrophil metabolism leading to NETosis and phagoytosis suppression. #microsky

pubs.acs.org/doi/10.1021/... #RNAmodifications

Molecular interplay between Integrative Mobile Elements exploiting Xer drives the evolution of cholera pandemics 🦠 www.biorxiv.org/content/10.6...

Ribosome hibernation in archea with HibA, with a bacterial line hpf/raiA domain rdcu.be/foDtr

Interspecies quorum sensing signals modulate multicellular organization and enhance contact-dependent antagonism in Vibrio cholerae www.nature.com/articles/s41... 🦠

Ribosomal allostery as a potential regulator of bacterial dormancy 🦠 www.nature.com/articles/s41...

Increased temperature selects for antibiotic resistance genes in a model aquatic ecosystem 🦠 www.nature.com/articles/s44...

TusDCB-mediated tRNA sulfur modification is required for virulence and intestinal fitness in enterohemorrhagic Escherichia coli 🦠 journals.asm.org/doi/10.1128/...

A transfer RNA inosine modification drives genome-wide synonymous recoding across human commensal bacterial families 🦠 academic.oup.com/pnasnexus/ar...

Unconventional monooxygenation by the O2-dependent tRNA wobble uridine hydroxylase TrhO www.nature.com/articles/s41... 🦠 cryo-electron microscopy structure of Bacillus subtilis TrhO–tRNAAla complex

Unconventional monooxygenation by the O2-dependent tRNA wobble uridine hydroxylase TrhO www.nature.com/articles/s41... 🦠 cryo-electron microscopy structure of Bacillus subtilis TrhO–tRNAAla complex

The Escherichia coli Radical SAM Enzyme YhcC Substitutes for the FAD-Dependent Oxidase Activity of MnmC in 5-Methylaminomethyl-2-Thiouridine tRNA Modification Under Anaerobic Conditions www.biorxiv.org/content/10.6... 🦠

Quantitative RNA pseudouridine landscape reveals dynamic modification patterns and evolutionary conservation across bacterial species. elifesciences.org/articles/107... 🦠

Second young talent taking the stage: Léo HARDY, Pasteur-Roux-Cantarini fellow from @zeynepbaharoglu.bsky.social lab at IBPC / @pasteur.fr He's diving into the molecular mechanisms by which ribosomal RNA modifications affect antibiotic tolerance. #AMR #RNA

🌍 First up: Keynote from Jean-Yves MADEC, One Health Coordinator at Anses-Inserm. He's taking the stage to talk about tackling AMR through a One Health lens — humans, animals, environment, all connected. #OneHealth #AMR

Now at the mic, François-Xavier WEILL from @institutpasteur.bsky.social He's presenting new findings on the identification and surveillance of a highly drug-resistant strain of Vibrio cholerae O1 El Tor. A global concern. #Cholera #AMR

Imane EL MEOUCHE from IAME @inserm.fr is now on stage, diving into how E. coli develops antibiotic tolerance and how cell division plays a role in urinary tract infections. #AMR #UTI

Christophe D'ENFERT from @institutpasteur.bsky.social and @inrae-france.bsky.social , tackling a too-often overlooked front in the AMR battle — antifungal resistance in pathogenic yeasts. #AMR #Fungi @ibeidlabex.bsky.social

Now @zeynepbaharoglu.bsky.social from IBPC @cnrs.fr and @pasteur.fr She's exploring how epitranscriptomic regulation controls bacterial response to antibiotics and adaptation to the host. #AMR #RNA @ibeidlabex.bsky.social

🎙️ We're live! @bastinlab.bsky.social & @zeynepbaharoglu.bsky.social are opening today's conference on antimicrobial resistance. A packed program ahead — stay tuned for live updates! #AMR #LiveConference

🦠 There is still time to register for #Vibrio2026! The meeting will take place in Berlin this September and will bring together the Vibrio community for exciting science and discussions. See you in Berlin!

Integrating mass spectrometry with Nanopore direct RNA sequencing for de novo modification profiling of bacteriophage MS2 www.biorxiv.org/content/10.6... 🦠

Extended Shine-Dalgarno motifs govern translation initiation in Staphylococcus aureus www.nature.com/articles/s41... 🦠

academic.oup.com/pnasnexus/ad... 🦠

Genome replication and cell division control by the methylation-sensitive GcrA regulator in Agrobacterium tumefaciens 🦠 www.biorxiv.org/content/10.6...

🦠 💫 www.science.org/doi/10.1126/...

Ribosome heterogeneity in E. coli : ribosomal RNA (rRNA) sequence and modifications, r-protein stoichiometry and PTMs, and ribosome-associated proteins pubs.acs.org/doi/10.1021/... 🦠

tRNA modifications profile in infective and noninfective forms of T. cruzi, by Kimura lab journals.plos.org/plospathogen...

Decoding universal principles of codon-mediated regulation of gene expression => model "codon weights" from transcriptomic data, for prediction of mRNA/prot abundance & expmtal validatn💫 "species-specific environments for gene expression are encoded in codon weights" www.biorxiv.org/content/10.6...

Our study comparing promoter existence and emergence in random vs genomic DNA is finally published!!! TLDR: the E. coli genome has more promoters than random DNA (even in intergenic regions), and promoters emerge ~3x more readily from random DNA than genomic DNA. (1/2)

Precision in RNA degradation: helicases, ribosome and RNA polymerase stalling, and RNA modifications: Trends in Cell Biology www.cell.com/trends/cell-...

Mapping the interactome of human tRNA methyltransferase TRMT1 using dual proximity labeling www.biorxiv.org/content/10.6...

Bridging the gap: When transcription meets translation (review) 🦠 doi.org/10.1111/febs...

Absence U34 thiolation and phenotypes in E. coli

Climate and cholera 🦠 www.nature.com/articles/s43...

Climate change and infectious diseases🦠 www.nature.com/articles/s41...

An RNA Language Model trained on sequence alone reveals the structural logic of Internal Ribosome Entry Sites www.biorxiv.org/content/10.6...

Structural modification of oxazolidinone antibiotics alters nascent peptide stalling preference and peptide trajectory through the ribosome www.biorxiv.org/content/10.6...

Codon degeneracy contributes to divergent fitness effects of rare tRNAs with A-starting anticodons www.biorxiv.org/content/10.6... => why are tRNAs with adenine at the wobble position (tRNA_ANN) are almost universally absent in nature ? mistranslation-related fitness costs... 🦠

𝘉. 𝘴𝘶𝘣𝘵𝘪𝘭𝘪𝘴 people take note👇 #MicroSky 🙏thx for pointing this out, ákos

Queuosine modification mediates cold-active growth in Shewanella glacialimarina www.biorxiv.org/content/10.6... 🦠 🥶 Q everywhere

Growth-dependent tRNA Reprogramming and Codon Bias Link Translation to Metabolic State in Enterococcus faecalis 🦠 queuosine Q again... (Q is also a japanese anime character) www.biorxiv.org/content/10.6...

My commentary (journals.asm.org/doi/10.1128/...) of the insightful paper from Dedon lab on tRNA modifs and EHEC virulence in response to host hormone (original article McShane et al: journals.asm.org/doi/10.1128/...) 🦠💫 #rnasky #rnabiology #microsky

RNA modification sequencing using mass spec explained 💫 #rnasky #rnabiology youtu.be/xtYW8YjfvwY?...