Delighted to announce that The International Meeting on the Biology of Vibrios will be back next year! Vibrio2026 will be held in Berlin in September 13-16. See you there Vibriologists! event.fourwaves.com/vibrio2026/p...

🚨New paper out! #MicroSky Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival. We expanded its genetic toolbox: 🧬promoters to fine-tune expression 🧬IPTG-inducible system 🧬CRISPRi for rapid knockdown bit.ly/46GUn2c 1/4

Two microbiome metabolites compete for tRNA modification to impact mammalian cell proliferation and translation quality control #rnasky www.nature.com/articles/s41...

🧬 Fresh RNA modifications newsletter out now: viral tRNA hijacking, hidden rRNA mods, dynamic Ψ, antibiotic resistance & more. 👉 open.substack.com/pub/rnamodif... #RNAsky #Epitranscriptomics #microsky #ribosome

Ribosomal RNA tentacles are targets of free radical damage in mammalian cells during oxidative and inflammatory stress #ribosome #rnasky www.biorxiv.org/content/10.1...

Idiosyncratic evolvability among single-point ribosomal mutants towards multi-aminoglycoside resistance 🦠 #ribosome dx.plos.org/10.1371/jour...

WarpDemuX-tRNA: barcode multiplexing for nanopore tRNA sequencing #rnasky💫👏 academic.oup.com/nar/article/...

Read this properly now and it really is a staggeringly beautiful study. And super important. Opens a new avenue of research I reckon. One of my papers of the year.

So excited our antibiotic potentiation story is out 🤩 Led by the extraordinary @manonlang.bsky.social with @fox-science.bsky.social & @amazeld.bsky.social +amazing collaborators @immunobladder.bsky.social @imaneelmeouche.bsky.social 🦠 We believe it can make a difference in #AMR infections!

when it comes to RNA-binding proteins, we prokaryotistas have to examples from 𝘌. 𝘤𝘰𝘭𝘪, long considered oddballs or remnants of the 'RNA world': · 4.5S RNA + 𝘧𝘧𝘩 protein, together forming the SRP (signal recognition particle) · 𝘳𝘯𝘱𝘈 protein + 𝘳𝘯𝘱𝘉 RNA, together forming RNase P #MicroSky #RNASky

New in JB: Gbolahan, Saxena et al. link membrane stress, the Rcs stress-response pathway and a block in chromosomal replication to the targeting of the DnaA initiator, likely via the ClpP protease. journals.asm.org/doi/10.1128/... @asm.org #JBacteriology

Comprehensive nucleoside analysis of archaeal RNA modification profiles reveals an m7G in the conserved P loop of 23S rRNA #rnasky #microsky 🦠💫 www.cell.com/cell-reports...

Deep mutational scanning of an S. pneumoniae FMN riboswitch reveals robustness during mouse infection but diverging adaptive landscapes in response to targeting antibiotics 🦠💫 #rnasky #microsky from Michelle Meyer lab www.biorxiv.org/content/10.1...

www.biorxiv.org/content/10.1... #microsky

Happy to be at the Microbes and RNA meeting at Strasbourg for inspiring talks and discussions. #RNA2025

🌟📢 The September 1st issue of RNA Modifications Digest is here 🧬 From frameshift-preventing tRNA mods to aa-tRNA-seq breakthroughs and hidden damage by Tgt, and check also the preprints. #rnasky #microsky 🦠💫 rnamodifupdates.substack.com/p/rna-modifi...

🌟📢 The September 1st issue of RNA Modifications Digest is here 🧬 From frameshift-preventing tRNA mods to aa-tRNA-seq breakthroughs and hidden damage by Tgt, and check also the preprints. 🦠💫 #microsku #rnasky open.substack.com/pub/rnamodif...

Structural insights into YheS-mediated release of SecM-arrested ribosome. www.biorxiv.org/content/10.1... 🦠 #ribosome #rnasky

Vibrio cholerae motility is associated with inter-animal transmission www.nature.com/articles/s41... 🦠

Mechanism of 30S subunit recognition and modification by the conserved bacterial ribosomal RNA methyltransferase RsmI #microsky #rnasky 🦠 www.biorxiv.org/content/10.1...

The tRNA dihydrouridine synthase DusA has a distinct mechanism in optimizing tRNAs for translation www.biorxiv.org/content/10.1... 🦠 #rnasky #microsky DusA enhances charging for some tRNAs with direct impact of D20 in translation. Not a broad chaperone. From Kothe lab with @koutmoulab.bsky.social 💫

journals.plos.org/plosbiology/... #microsky

Robust antibiotic sensitization of pathogenic Pseudomonas aeruginosa via negative hysteresis in the cell envelope. 🦠with Cpx, beta-lactams and aminoglycosides www.biorxiv.org/content/10.1...

Now we need the #epitranscriptomic version 🤷‍♀️🤪

OMG this is it?! The final piece in the puzzle?!😲 tRNAs are the key, because archaic tRNAs sit at the core of all ribosomes. If aminoacyl-tRNAs could form on early Earth, you have a plausible route for the first self-replicators, the bridge from chemistry to biology www.nature.com/articles/d41...

Happy to announce the latest preprint of PhD candidate Mariana Abakarova (not yet on bluesky) tinyurl.com/4ewd2v85 in co-supervision with Prof. @laineelodie.bsky.social @sorbonne-universite.fr / @upariscite tinyurl.com/2ntnfvw3 Funded by @agencerecherche.bsky.social ADAGIO and @erc.europa.eu PROMISE

Proximity-specific ribosome profiling using light induced BirA. 😎 #Ribosome #RNASky

On phages, pilus, and mythological father-son relationships #microsky

Very happy to see this one out: doi.org/10.1093/molbev/msaf192 Here, we assessed the contribution of natural transformation to the acquisition of novel genes. See preprint thread. @molbioevol.bsky.social #microsky #evobio

E. Coli with linear growth 🦠🤯 #micdosky "linear proliferation for a finite number of generations" where only one daughter cell continues to divide, with split cyaA making cAMP only in one daughter cell. www.biorxiv.org/content/10.1...

Review: Functions and therapeutic applications of pseudouridylation #rnasky tRNA, rRNA, mRNA www.nature.com/articles/s41...

Artificially modulating tRNA availability boost translation - Chemically synthesized tRNAs with multiple modifications, exhibit ~4-fold higher decoding efficacy than unmodified tRNAs #microsky #rnasky 💫 doi.org/10.1038/s414...

(p)ppGpp-dependent activation of gene expression during nutrient limitation | mBio #microsky #ribosome with Hpf and friends journals.asm.org/doi/10.1128/...

🚨 New Preprint Alert 🚨 Our latest work, A Zur-dependent regulatory RNA involved in maintaining zinc homeostasis in Staphylococcus aureus, is now live on bioRxiv! 🧬🦠📄 Check it out here: www.biorxiv.org/content/10.1...

#microsky #rnasky 💫🦠

"Antibiotic-resistant Gram-negative bacteria (including K pneumoniae, Acinetobacter spp, and Escherichia coli), as well as rifampicin-resistant Mycobacterium tuberculosis, were ranked in the highest quartile" #microsky #amr

Simultaneous detection of RNA modifications using In Vitro Transcription and Direct RNA Nanopore sequencing #rnasky #rnamodification www.biorxiv.org/content/10.1...

Adaptive genetics reveals constraints on protein structure/function by evolving E. coli under constant nutrient limitation #microsky 🦠 bmcbiol.biomedcentral.com/articles/10....

www.nature.com/articles/s41... #microsky 🦠 model in which the entire cell envelope, including the periplasm, collectively enables Gram-negative bacteria to overcome osmotic challenges

Aggresomes protect mRNA under stress in Escherichia coli www.nature.com/articles/s41... "prolonged stress leading to ATP depletion in Escherichia coli results in increased aggresome formation, compaction and selective mRNA enrichment within these aggresomes" 🦠 #microsky #rnasky

A new methods paper from us, this time with a focus on reliable monitoring membrane potential and cell lysis at different growth stages and cell densities. Also, note the surprisingly high level of background lysis in seemingly healthy B. subtilis cultures. www.biorxiv.org/content/10.1...

An Expanded Repertoire of tRNA Sources for Cell-Free Protein Synthesis doi.org/10.1101/2025...

As part of the ENW-XL UTIr project we have developed a novel fully defined synthetic artificial urine medium for urinary bacteria. www.biorxiv.org/content/10.1... #UTISky #AMR #MicroSky #MicrobiomeSky #InfectiousDiseases #ClinMicro

Got an invite to publish in what looks like the dystopian microbiology journal 🦠 … tempting 😅 #microsky

Machine learning in understanding environmental variability of vibriosis in coastal waters 🦠 #microsky #vibrio temperature, salinity, and phytoplankton: important environmental parameters for vibrio proliferation journals.asm.org/doi/10.1128/...

Climate change and Vibrio: Environmental determinants for predictive risk assessment | PNAS #microsky #vibrio 🦠 www.pnas.org/doi/10.1073/...

An RNA modification prevents extended codon-anticodon interactions from facilitating +1 frameshifting #rnasky #microsky 🦠 m1G (TrmD) at position 37 modulates tRNA conformation on ribosome and suppresses +1 FS induced by tRNA-Pro (four base pair codon-anticodon)💫 smFRET & cryo doi.org/10.1038/s414...