🌸🐟 April RNA mods issue: reversible ac4C, Q-driven phenotypes, Ψ everywhere, grandpa ribosomes (aging ones), and translation quietly running the show (maybe the ribosome is the boss after all 👀) rnamodifupdates.substack.com/p/rna-modifi...

Rsfs dissociates vacant 70S ribosomes www.biorxiv.org/content/10.1... 🦠Bacteria adapt nutrient deprivation by reducing vacant 70S ribosomes via the silencing factor RsfS

Slowing the ribosome to allow survival in stressful conditiins www.biorxiv.org/content/10.6... The Helicobacter pylori ribosomal silencing factor RsfS is required for low-growth states and chronic infection 🦠

So happy to see this finally published in @plosbiology.org! This is the first chapter of @julielebris.bsky.social PhD thesis demonstrating how capsules are exchanged by plug-and-play dynamics w/ @epcrocha.bsky.social #microsky @klebclub.bsky.social Link below- Check out her 🧵 for more details!

Permuted 23S rRNA is integrated in 50S ribosome particles in Thermococcus barophilus www.biorxiv.org/content/10.6...

Unveiling hidden variables in stressed bacteria: Cell Reports 🦠https://www.cell.com/cell-reports/fulltext/S2211-1247%2826%2900044-6

Mechanism of 30S subunit recognition and modification by the conserved bacterial ribosomal RNA methyltransferase RsmI | PNAS 🦠🔥https://www.pnas.org/doi/10.1073/pnas.2523453123

Eukaryotic ribosomes also hibernate and SNORe.. identification of a new hibernation factor in yeast and fungi, which interacts with rRNA #ribosome 💫

Two post-doctoral positions open in our lab. We're looking for people with expertise in molecular microbiology and/or genome-scale tools. Initially 3 yrs, posts could be extended for upto 8 yrs. If you'd like to know more about us take a look at www.graingerlab.com www.jobs.ac.uk/job/DQY475/r...

Drought drives elevated antibiotic resistance across soils @natmicrobiol.nature.com from Dianne Newman with @hannahjeckel.bsky.social www.nature.com/articles/s41...

Mistranslation from an endogenous tRNA variant in human pan-genome cell lines doi.org/10.1093/nar/... " a natural genome-encoded human tRNA mutant causes mistranslation in cells derived from healthy individuals" with impact on modification and fragmentation #RNAsky

A universal regulatory mechanism for prevention of replication restart from RNA:DNA hybrids 🦠 from Merrikh lab www.biorxiv.org/content/10.6...

Purification of native tRNAs from E. coli with their post-transcriptional modifications and translational activity 🦠 #rnasky #rnabiology academic.oup.com/nar/article/...

days apart from this: academic.oup.com/pnasnexus/ar...

On #ribosome addiction and early life 🌌 arxiv.org/abs/2602.23268 by @mkrupovic.bsky.social

#AMR - we are rapidly increasing the volume of resistance genes, a huge threat to #PublicHealth #AntimicrobialResistance www.theguardian.com/environment/...

non-correlating mRNA-protein levels during stress 🦠

"Coastal Vibrio harbor a high diversity of ARGs. Their selective maintenance in the absence of antibiotic selection is puzzling. While their mobilization is rare, it may just be a question of time when the ecological context turns the coastal environment into a source for ARG spillover." 🦠🧨#AMR

Interesting human study here on material immunity protecting neonates from E. coli sepsis...with an interesting preclinical mouse piece showing that probiotic E. coli Nissle 1917 introduced preconceptually into intestine can prime useful IgG protective response in neonates #MicroSky #UTISky #IDSky

Specialised #ribosomes in plants

tRNA modifications enable codon usage signatures to coordinate bacterial virulence in Salmonella 🦠💫https://www.biorxiv.org/content/10.64898/2026.02.22.707322v1

Some molecular machines, like ribosomes, can persist for long periods of time in cells. Could molecular aging of ribosomes shape how proteins are made? In our new preprint we track ribosomes as they age in cells and uncover unexpected effects on translation (1/10) www.biorxiv.org/content/10.6...

"The collision propensity of specific #ribosome subpopulations increases with molecular age."

Queuosine promotes wecB-dependent phage resistance and biofilm formation in marine bacterium Shewanella glacialimarina 🦠💫 www.biorxiv.org/content/10.6...

Pseudouridine selects RNAs for extracellular transport www.biorxiv.org/content/10.1...

Rifampicin-induced Staphylococcus aureus persister formation is driven by CodY regulon and oxidative stress level 🦠https://www.biorxiv.org/content/10.64898/2026.03.03.709237v1

RNA from scratch.. 💫 Interstep compatibility of a model for the prebiotic synthesis of RNA consistent with Hadean natural history | PNAS www.pnas.org/doi/10.1073/...

Stress 🧨, bacteria 🦠, Fe-S by @sronneau.bsky.social lab

RNA modifications are not static decorations, they are dynamic regulatory determinants. 🦠 The RNA modifications newsletter for March is out 👉https://rnamodifupdates.substack.com/p/rna-modifications-newsletter-march

multi resistance dissemination with rRNA modification enzyme on mobile genetic element 🦠 #AMR #RNAbiology #rnasky

"targeting nucleotide synthesis synergizes with β-lactam-mediated killing in hypertolerant V. cholerae, Klebsiella pneumoniae and E. coli." 🦠

Welcome to bluesky EGM unit 😊🦠 bsky.app/profile/egmr...

Quantitative RNA pseudouridine landscape reveals dynamic modification patterns and evolutionary conservation across bacterial species 🦠https://www.biorxiv.org/content/10.1101/2025.05.13.653745v2.full

Mechanism of 30S subunit recognition and modification by the conserved bacterial ribosomal RNA methyltransferase RsmI 🦠 www.biorxiv.org/content/10.1...

On the need for more research into the relationship between climate change and antimicrobial resistance. 🦠 #AMR

Cytosine methylation contributes to the fitness of Caulobacter cells naturally expressing a Vsr-like protein: iScience www.cell.com/iscience/ful... 🦠

🦠💫 RNA modifications and stress

⛸️🧬 February RNA Modifications Newsletter From CRISPR-triggered tRNA tail cleavage to temperature-responsive rRNA modifications, ribosome QC, and pathway-specific rRNA modification during biogenesis. #RNAmods #Epitranscriptomics #Ribosome #Translation #tRNA #rRNA

🦠 #rnasky #rnabiology

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accumulation of the 50S subunit in delta-tlyA with cold sensitive phenotype and resistance to aminoglycosides 🦠 #RNAmodifications #RNAbiology #rnasky

Signature codon usage patterns define functional gene families, this is in yeast and could be also very informative for bacteria and link with tRNA modifs #rnasky #codonusage #rnabiology #rnamodifications

trans-Translation inhibitors that kill M. tuberculosis and pathogenic Non-tuberculous Mycobacteria have a dual mechanism of action www.biorxiv.org/content/10.6...

Structure of the 30S translation initiation complex coupled to paused RNA polymerase and its potential for riboregulation #rnasky 🦠

Probing the epitranscriptome and RNA damage with nanopore direct RNA sequencing #tnasky

'This video is the culmination of several yrs attempting to: (1) Figure out best practices for modeling ptn-ptn interactions; (2) Understand the outputs of programs like AlphaFold & adjacent software including quantitative metrics;(3) Communicate my thoughts to unwitting victims through workshops'

A to I RNA editing at the replication fork #RNAsky #RNAbiology

DNA modif and phages 🦠