Satpal (Sati) Jhujh
@drjhujh.bsky.social
📤 760
📥 682
📝 18
Interested in all things DNA Replication, DNA repair and human disease!
pinned post!
So proud to have co-authored this paper with
@sorenhough.bsky.social
identifying mutations in MAEA, an E3 ligase, as underlying a novel disease resulting from defective HR-dependent replication fork protection/restart 🥳 Grateful to everyone that contributed, incl. the patients & their families!
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13 days ago
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Satpal (Sati) Jhujh
Tatiana Moiseeva
14 days ago
Ever wondered how TIMELESS/TIPIN can sit at the leading edge of the replication fork regulating its speed, but also bind polymerases and ssDNA/RPA in replication stress? We have an idea how it might work! Check out our new preprint, any feedback is highly appreciated!
www.biorxiv.org/content/10.6...
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So proud to have co-authored this paper with
@sorenhough.bsky.social
identifying mutations in MAEA, an E3 ligase, as underlying a novel disease resulting from defective HR-dependent replication fork protection/restart 🥳 Grateful to everyone that contributed, incl. the patients & their families!
add a skeleton here at some point
13 days ago
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Satpal (Sati) Jhujh
CortésLab
3 months ago
Kickstarting our Bluesky🦋 account with great news! Our REPAIRome study finally out today in
@science.org
www.science.org/doi/10.1126/...
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A comprehensive genetic catalog of human double-strand break repair
The analysis of DNA sequence outcomes provides molecular insights into double-strand break (DSB) repair mechanisms. Using parallel in-pool profiling of Cas9-induced insertions and deletions (indels) w...
https://www.science.org/doi/10.1126/science.adr5048
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Satpal (Sati) Jhujh
Alberto Marin
28 days ago
Thrilled to share that my postdoc research is published today in
@science.org
! We found that DNA repair uses cohesin complexes to build new chromatin loops that guide the homology search and boost accurate repair! 1/n
www.science.org/doi/10.1126/...
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Cohesin drives chromatin scanning during the RAD51-mediated homology search
Cohesin folds genomes into chromatin loops, the roles of which are under debate. We found that double-strand breaks (DSBs) induce de novo formation of chromatin loops in human cells, with the loop bas...
https://www.science.org/doi/10.1126/science.adw1928
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Satpal (Sati) Jhujh
Alison Harrod
27 days ago
Our new review, looking into the roles of SWI/SNF in the chromatin environment around centromeres, is just out in Open Biology!
@kezlane.bsky.social
@thedownslab.bsky.social
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Satpal (Sati) Jhujh
MRC Laboratory of Molecular Biology
9 months ago
How do SMC complexes capture DNA? 🧪 Led by Frank Bürmann, Jan Löwe’s & Mark Dillingham’s groups have identified the directional loading mechanism the bacterial SMC complex MukBEF uses to capture & ingest DNA, & found a phage inhibitor to this pathway. Read more:
tinyurl.com/4s8nrrkk
#LMBResearch
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Satpal (Sati) Jhujh
Luke Greenhough
about 2 months ago
Excited to share our discovery of the RAD51 filament cap, built by a newly identified RAD51 paralog complex 🔬🧬
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Satpal (Sati) Jhujh
2 months ago
New preprint from the lab for any DNA repair or replication stress geeks out there!
www.biorxiv.org/content/10.1...
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A nuclease-driven mechanism of post-replicative ssDNA gap suppression.
The persistence of post-replicative ssDNA gaps following PRIMPOL-mediated replication repriming is linked to chemosensitivity, and in all models reported to date the nuclease MRE11 has been implicated...
https://www.biorxiv.org/content/10.1101/2025.10.22.683685v1
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Satpal (Sati) Jhujh
Basil Greber
3 months ago
Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In
@vcushing.bsky.social
's magnum opus, we use
#cryoEM
to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...
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Structural basis of T-loop–independent recognition and activation of CDKs by the CDK-activating kinase
Cyclin-dependent kinases (CDKs) are prototypical regulators of the cell cycle. The CDK-activating kinase (CAK) acts as a master regulator of CDK activity by catalyzing the activating phosphorylation o...
https://www.science.org/doi/10.1126/science.adw0053
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Satpal (Sati) Jhujh
Sandra Segura-Bayona
3 months ago
🧬 Preprint alert! "Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity." Read the manuscript here 👉
www.biorxiv.org/content/10.1...
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Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity
Mutations in the ATRX chromatin remodeller predispose to a developmental genetic disorder and cancer, but how it safeguards genome and telomere stability remains unresolved. Here, we uncover critical ...
https://www.biorxiv.org/content/10.1101/2025.09.22.677761v1
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Satpal (Sati) Jhujh
Science Magazine
3 months ago
"… as I helped this bright young scholar rediscover hope in her journey, I rediscovered my own."
#ScienceWorkingLife
https://scim.ag/473CI43
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Satpal (Sati) Jhujh
Yasu Daigaku
4 months ago
Our preprint on Pol-η usage during genome replication has been uploaded. It addresses the biased use of Pol-η in the lagging strand and late-replicating regions, which may be correlated with cancer mutagenesis.
www.biorxiv.org/content/10.1...
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Strand- and replication timing-dependent functions of DNA polymerase η in human DNA replication and mutagenesis.
Efficient eukaryotic DNA replication relies on the coordinated actions of replicative and error-prone polymerases, with the latter providing flexibility at the cost of mutagenesis. DNA polymerase η (P...
https://www.biorxiv.org/content/10.1101/2025.09.10.675280v1
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Satpal (Sati) Jhujh
bioRxiv Molecular Biology
4 months ago
DDIAS is a single-stranded DNA-binding effector of the TOPBP1-CIP2A complex in mitosis
https://www.biorxiv.org/content/10.1101/2025.09.09.675193v1
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Satpal (Sati) Jhujh
Elphege Nora Lab at UCSF
5 months ago
New preprint with
@gfudenberg.bsky.social
We find the rate of cohesin loop extrusion in cells is set by NIPBL dosage and tunes many aspects of chromosome folding. This provides a molecular basis for NIPBL haploinsufficiency in humans. 🧵👇
www.biorxiv.org/content/10.1...
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NIPBL dosage shapes genome folding by tuning the rate of cohesin loop extrusion
Cohesin loop extrusion is a major driver of chromosome folding, but how its dynamics are controlled to shape the genome remains elusive. Here we disentangle the contributions of the cohesin cofactors ...
https://www.biorxiv.org/content/10.1101/2025.08.14.667581v1
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Satpal (Sati) Jhujh
Uli Rass
4 months ago
My first post on bsky to share our latest research in
@nature.com
: “DNA2 enables growth by restricting recombination-restarted replication” - defines DNA2’s essential function & elucidates links with primordial dwarfism and cancer. Thanks to the lab, collaborators &
@ukri.org
@acmedsci.bsky.social
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DNA2 enables growth by restricting recombination-restarted replication - Nature
DNA2 suppresses recombination-restarted replication and checkpoint activation at stalled forks, and its loss triggers recombination-dependent synthesis, checkpoint signalling and cell-cycle exit, high...
https://www.nature.com/articles/s41586-025-09470-5
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Satpal (Sati) Jhujh
Andrew Deans
8 months ago
Some seriously nice biochemistry and cryo-EM of FAN1-PCNA-flapDNA complexes!
www.nature.com/articles/s41...
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Satpal (Sati) Jhujh
bioRxiv Molecular Biology
8 months ago
Slow RNAPII elongation enhances naive-pluripotency rewiring while preserving replication fork speed
https://www.biorxiv.org/content/10.1101/2025.05.12.653265v1
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Satpal (Sati) Jhujh
Deegan Lab
9 months ago
3rd edition of the fantastic UK DNA replication meeting is happening next September in Edinburgh. Don't miss out 🧬🏴
www.biochemistry.org/events-and-t...
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UK DNA Replication Meeting 2026
Research in the DNA replication field is progressing at a remarkable rate. Technological innovations in biochemical reconstitution, structural biology, proteomics and DNA sequencing are helping to rap...
https://www.biochemistry.org/events-and-training/events-calendar/uk-dna-replication-meeting-2026/
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🎉 Congratulations! Great to see this out in print! 👏
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9 months ago
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Satpal (Sati) Jhujh
9 months ago
Pls repost. Postdoc role on post-replicative ssDNA gap processing. Interesting models that should float replication aficionados boats. DM or
[email protected]
for info. Until Dec26 but will strive for funding to provide continuity. Fantastic place to live+work
www.jobs.ac.uk/job/DMG682/r...
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Satpal (Sati) Jhujh
altmeyerlab
10 months ago
How are chromosome breaks repaired and how is their repair influenced and facilitated by nuclear and genome dynamics? Fantastic initiative and collaboration with
@karimmekhail.bsky.social
,
@chiololab.bsky.social
, and
@lablegube.bsky.social
.
@natrevmcb.bsky.social
Link to article:
rdcu.be/edViW
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Nuclear and genome dynamics underlying DNA double-strand break repair
Nature Reviews Molecular Cell Biology - Changes in nuclear and genome organization promote the repair of DNA double-strand breaks and genome stability. Processes that are involved include the...
https://rdcu.be/edViW
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Satpal (Sati) Jhujh
James Dewar
10 months ago
🧬⛓️ Our latest preprint examines the fate of collapsed forks in vertebrates 🐸 and our colleagues in
@heathmurraylab.bsky.social
asked similar questions in bacteria 🦠 to reveal how evolution shapes the response to fork collapse (see🧵)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
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Resolution of collapsed forks is separate from completion of DNA synthesis
Replication fork collapse at single-strand DNA breaks (SSBs) poses a serious threat to genome stability. Using Xenopus egg extracts, we show that a replication fork encountering an SSB on either the l...
https://www.biorxiv.org/content/10.1101/2025.03.03.641087v1
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Satpal (Sati) Jhujh
bioRxiv Cancer Bio
11 months ago
TRIP13 protects pancreatic cancer cells against intrinsic and therapy-induced DNA replication stress
https://www.biorxiv.org/content/10.1101/2025.01.26.634889v1
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Satpal (Sati) Jhujh
bioRxiv Cell Biology
12 months ago
Telomeric SUMO level influences the choices of APB formation pathways and ALT efficiency
https://www.biorxiv.org/content/10.1101/2025.01.16.633463v1
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Satpal (Sati) Jhujh
Max-Planck-Gesellschaft
12 months ago
Okay, the list is far from complete, but it is a first attempt.☺️Here you can find our starter pack on
#research
organizations and
#academies
, in
#Germany
and worldwide. Who else do you think should be included?
go.bsky.app/DGGtVu6
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Satpal (Sati) Jhujh
Cees Dekker
12 months ago
New discovery from our
#CDlab
reported in Cell
@cp-cell.bsky.social
today: SMC motor proteins have a left-right ‘gearbox’ and can switch direction in DNA loop extrusion!
www.sciencedirect.com/science/arti...
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Satpal (Sati) Jhujh
bioRxiv Cell Biology
12 months ago
The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain
https://www.biorxiv.org/content/10.1101/2025.01.10.632395v1
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Satpal (Sati) Jhujh
bioRxiv Cell Biology
12 months ago
Identification of critical factors of the replication stress response in human cells
https://www.biorxiv.org/content/10.1101/2025.01.12.632149v1
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Satpal (Sati) Jhujh
Tatiana Moiseeva
12 months ago
Fascinating paper! No G2/M checkpoint for under-replicated cells!
academic.oup.com/nar/article/...
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Cell cycle progression of under-replicated cells
Abstract. Cell cycle checkpoints are the regulatory mechanisms that secure the strict order of cellular events for cell division that ensure genome integri
https://academic.oup.com/nar/article/53/1/gkae1311/7945391
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Satpal (Sati) Jhujh
Ester Hammond
12 months ago
Great start to 2025. Tumor hypoxia promotes immune evasion by autophagy-mediated downregulation of MHC class I expression leading to reduced antigen presentation. Great work from
@halaestephan.bsky.social
www.embopress.org/doi/full/10....
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Hypoxia promotes tumor immune evasion by suppressing MHC-I expression and antigen presentation | The EMBO Journal
imageimageHypoxia contributes to cancer resistance to both standard therapies and immunotherapy. This work demonstrates that tumor hypoxia promotes immune evasion by autophagy-mediated downregulation ...
https://www.embopress.org/doi/full/10.1038/s44318-024-00319-7
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Satpal (Sati) Jhujh
Grant Stewart
12 months ago
Great paper about p38 and endometrial cancer from a long-term collaborator and friend, Cyrus Vaziri, just published in Cell Reports:
www.cell.com/cell-reports...
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MAPK14/p38α shapes the molecular landscape of endometrial cancer and promotes tumorigenic characteristics
Using unbiased CRISPR-Cas9-based genetic screens, Joseph et al. identify MAPK14/p38α as a dependency and vulnerability of high-grade endometrial cancer (HGEC) metastatic spheroids. MAPK14/p38α critically programs the phosphoproteome, transcriptome, and metabolome of HGEC spheroids, promoting cancer stemness and tumorigenicity.
https://www.cell.com/cell-reports/fulltext/S2211-1247(24)01455-4
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Satpal (Sati) Jhujh
Néstor García-Rodríguez
about 1 year ago
Our last article about ssDNA gaps and BRCA1 👇🏻👇🏻
doi.org/10.1093/nar/...
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EXO1 and DNA2-mediated ssDNA gap expansion is essential for ATR activation and to maintain viability in BRCA1-deficient cells
Abstract. DNA replication faces challenges from DNA lesions originated from endogenous or exogenous sources of stress, leading to the accumulation of singl
https://doi.org/10.1093/nar/gkae317
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Satpal (Sati) Jhujh
Drew Berry wehi.tv
about 1 year ago
Delighted to publish my new molecular animation: DNA Break Repair by Homologous Recombination
youtu.be/Xe-83tBcxhs
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DNA Break Repair by Homologous Recombination (2024) Drew Berry wehi.tv
YouTube video by WEHImovies
https://youtu.be/Xe-83tBcxhs
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Satpal (Sati) Jhujh
Chris Carnie
about 1 year ago
Happy to share a review written with the wonderful
@jstingele.bsky.social
on transcription-coupled DPC repair, featuring an overview of new methods, close comparison with TC-NER, comments on clinical implications and plenty of discussion of the unknowns.
www.cell.com/trends/cell-...
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Transcription-coupled repair of DNA–protein crosslinks
DNA–protein crosslinks (DPCs) are highly toxic DNA lesions that are relevant to multiple human diseases. They are caused by various endogenous and environmental agents, and from the actions of enzymes...
https://www.cell.com/trends/cell-biology/fulltext/S0962-8924(24)00234-4
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Satpal (Sati) Jhujh
Nik Copeland
about 1 year ago
Not sure if there are PhG applicants on here, but if there are there is an information event for UKRI funded PhD projects at northwest Bio here. Please sign up if interested.
t.co/yIgIyelZFP
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https://www.eventbrite.co.uk/e/1095142052359?aff=oddtdtcreator
https://t.co/yIgIyelZFP
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Re posting this as there's been a lot of updates since I originally posted the starter pack!
add a skeleton here at some point
about 1 year ago
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Satpal (Sati) Jhujh
Tatiana Moiseeva
about 1 year ago
ok, Genome Stability people. Here are our two starter packs - since I can't pin them both independently to my profile, have to make a new post. Still adding new people to the second pack. As usual, please reach out if you would like to be added!
bsky.app/starter-pack...
bsky.app/starter-pack...
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Satpal (Sati) Jhujh
Mark Rubin
about 1 year ago
BlueSky: A Guide for Scientists by
@jacoates.bsky.social
#AcademicSky
#EduSky
🧪
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Satpal (Sati) Jhujh
Román González-Prieto
about 1 year ago
The lab 2024 article is out! BRCA1/BARD1 ubiquitinates PCNA to promote continuous, ssDNA-free, DNA synthesis in a RAD18-independent pathway.
www.nature.com/articles/s41...
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BRCA1/BARD1 ubiquitinates PCNA in unperturbed conditions to promote continuous DNA synthesis - Nature Communications
Here the authors identify PCNA, a master regulator of DNA replication, as a ubiquitin substrate for the BRCA1/BARD1 heterodimer. This modification is crucial to avoid the appearance of unreplicated DN...
https://www.nature.com/articles/s41467-024-48427-6
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go.bsky.app/KxWyLE4
Trying to put together a starter pack for journals!
add a skeleton here at some point
about 1 year ago
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Satpal (Sati) Jhujh
Tycho Mevissen
about 1 year ago
Let's get this site started. I'm excited to share that our work on transcription-coupled DNA repair is now published! We recapitulated TC-NER in Xenopus egg extract and identified STK19 as a core repair factor that couples lesion-stalled RNA Pol II to TFIIH. 🧬🐸 ➡️
cell.com/cell/fulltex...
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STK19 positions TFIIH for cell-free transcription-coupled DNA repair
Together with structural analyses, a cell-free system for transcription-coupled nucleotide excision repair (TC-NER) shows that STK19 is an integral component of the TC-NER complex that couples RNA pol...
https://cell.com/cell/fulltext/S0092-8674(24)01202-9
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Satpal (Sati) Jhujh
Nature Portfolio
about 1 year ago
We said hello to our new followers last week, but there's many more of you now! Welcome! We've created a starter pack for you, which is a work in progress. We'd also love to hear from you about your work we've published or what kind of things you want us to post.
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Satpal (Sati) Jhujh
Francesca Mattiroli
about 1 year ago
The peer-reviewed version of our paper is out! Thanks to all co-authors, editor and reviewers for the smooth process 🤓🎉 And don’t miss the chromatin haiku!
www.cell.com/molecular-ce...
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Nice to see the number of people on here increasing over the last few days. 🎉
about 1 year ago
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Satpal (Sati) Jhujh
Tatiana Moiseeva
about 1 year ago
ok, I tried to make a starter pack on genome stability/DNA replication/DNA repair etc. Sorry if I couldn't find someone, please nominate yourself and your colleagues in comments or DMs, looking forward to connecting with everyone!
go.bsky.app/9jdDNxG
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Satpal (Sati) Jhujh
Richard Sever
about 1 year ago
Reminder for newcomers that bioRxiv has Bluesky accounts in every subject category - great way to keep up (please re-skeet)
connect.biorxiv.org/news/2023/09...
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bioRxiv expands on Mastodon and Bluesky
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
https://connect.biorxiv.org/news/2023/09/27/biorxiv_expands_on_social_platforms
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Maiden BlueSky post to rep a collaborative paper I'm a co-author from the Stewart and Vazri lab showing that RNF168 has roles in both DSB repair and DNA replication! Really glad to have been part of this and super excited for people to read it! 😅
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PCNA-binding activity separates RNF168 functions in DNA replication and DNA double-stranded break signaling
Abstract. RNF168 orchestrates a ubiquitin-dependent DNA damage response to regulate the recruitment of repair factors, such as 53BP1 to DNA double-strand b
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae918/7833678
about 1 year ago
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