James Dewar (@jamesmdewar.bsky.social)

In our latest preprint we analyzed how abasic (AP) sites affect DNA replication: - AP sites stall DNA synthesis ⛓️‍💥 - Leading APs stall replisomes but lagging APs don’t 🛑/✅ - TLS is crucial for bypass of both leading and lagging strand AP sites 🛠️🧬 www.biorxiv.org/content/10.1...

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Leading and lagging strand abasic sites differentially affect vertebrate replisome progression but involve analogous bypass mechanisms Abasic sites are one of the most frequent forms of DNA damage that interfere with DNA replication. However, abasic sites exhibit complex effects because they can be processed into other types of DNA d... https://www.biorxiv.org/content/10.1101/2025.01.09.632187v1