First NIH grant for the lab!! Very nice way to start the week :)

Students need to remember that Inigo Montoya method for emails and greetings: "Hello, my name is Inigo Montoya. You killed my father. Prepare to die.” Polite Greeting Name Relevant Personal Link Manage Expectations Keep it BRIEF.

New preprint🎉 Happy to share our collaborative manuscript, where we provide in vivo and genome-wide evidence linking MCM2-7 helicase activation, DNA extrusion to initial origin melting, thereby adding a new mechanistic layer to how eukaryotic DNA replication is initiated. 👉 doi.org/10.64898/202...

My cell has (is?) a foot!

Read our latest work - a comprehensive review on how DNA secondary structures affect replication. Very proud of Adi, Maria and Billie for their monumental effort. They've covered a large body of literature while proposing updated models. Open Access in DNA Repair: doi.org/10.1016/j.dn...

Ever wondered how TIMELESS/TIPIN can sit at the leading edge of the replication fork regulating its speed, but also bind polymerases and ssDNA/RPA in replication stress? We have an idea how it might work! Check out our new preprint, any feedback is highly appreciated! www.biorxiv.org/content/10.6...

Inhibition of lagging strand replication by G-rich telomeric DNA and the shelterin subunit POT1 https://www.biorxiv.org/content/10.1101/2025.11.13.688213v1

Check out our new biosensor technology to study DDR kinase signaling: ProKAS. We combine: -proteomics -engineered peptide sensors -a new concept of amino acid barcodes ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data. Just published today: rdcu.be/ePNo0

In praise of fundamental research Our editorial this week argues that I n these financially straitened times, funders must recognize that great discoveries often arise from work that was looking for something completely different 🧪 @nature.com www.nature.com/articles/d41...

Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...

Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up! www.biorxiv.org/content/10.1... A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)

When your incoming lab member's H1B was approved yesterday, but now they can't travel to the US...

Thanks CSHL for the photos, my facial expressions on them are priceless. I think this one is a keeper for replying to a lot of stuff on the internets...

Excited to be back at the CSHL meeting on DNA replication this week! Campus views are amazing as always, looking forward to all the science!

Checkout our latest research in @natcomms.nature.com rdcu.be/eBqBI A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells. A collaboration with Mike Boemo’s team

How are Rad51 filaments formed during Homologous Recombination? Jaigeeth Deveryshetty and Ayush Mistry describe the mechanism through the lens of Rad52, the yeast homolog of human BRCA2. urldefense.com/v3/__https:/...

Our new review on how the initiation of DNA replication is coordinated with transcription is now online! Congratulations to @syedshahidmusvi.bsky.social! www.sciencedirect.com/science/arti...

It is finally out 👍😀. Great job of the team (mostly absent from Bsky) and @m-saponaro.bsky.social CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...

Check out this new review (with animations, natch) of mechanisms of licensing origins of DNA replication - a wonderful (and continuing!) collaboration with Bruce Stillman @cshlnews.bsky.social and John Diffley @crick.ac.uk! www.nature.com/articles/s41...

Sameera Vipat, the first PhD student from our lab, successfully defended her thesis in Tallinn yesterday! Congratulations to Sameera, and a huge thank you to @lmyler.bsky.social for being an opponent and a great scientific discussion!

We carried an agarose/EtBr gel to the imager on a plastic box cover that was labeled with red sharpie. The gel acquired a nice but unexpected label. How did this happen? The box was not imaged. Sharpies don't get washed off that easily. What kind of sorcery is this? The label says "biotin HA 6/6"

🧪 Attention those with terminated grants, including for NSF, NASA, EPA, NIH, etc: Let Congress know about the impact of terminations via this form: democrats-science.house.gov/grantcancela... Only Dem staff on the House Science Committee will have access (unless if you explicitly state otherwise).

Excited to share our latest preprint by @mihyun-kim.bsky.social showing how DNA incision activities in nucleotide excision are regulated to ensure genomic stability. We show that interactions between TFIIH and XPG control the 5' incision by ERCC1-XPF. www.biorxiv.org/content/10.1...

Delighted to share that the results of my main postdoc project are now published in @nature.com 🔗 doi.org/10.1038/s415...

Some seriously nice biochemistry and cryo-EM of FAN1-PCNA-flapDNA complexes! www.nature.com/articles/s41...

Hey everyone! Every year our HIllman Cancer Center organizes a fundraising bike ride to raise money for cancer research. Sad, but currently it is especially relevant. If you like this cause, please consider supporting our Genome Stability team! fundraisers.rushtocrushcancer.org/tatiana-mois...

A huge thank you to our speakers @kajastelab.bsky.social and Raphael Ceccaldi for their amazing talks and fascinating research at yesterday's webinar! Our next webinar is on June 3rd featuring @agagambus.bsky.social and @chowdhurylab.bsky.social. Join our community: docs.google.com/forms/d/e/1F...

24th Annual Midwest DNA Repair Symposium University of Michigan May 17-18, 2025, Ann Arbor sites.google.com/umich.edu/um...

🔬 New from the Farnung Lab: We established a fully in vitro reconstituted chromatin replication system and report the first cryo-EM snapshots of the human replisome engaging nucleosomes. Brilliant work by @felixsteinruecke.bsky.social with support from @jonmarkert.bsky.social! tinyurl.com/replisome

Happy to post our first preprint in a while! www.biorxiv.org/content/10.1.... This was a slightly new direction for us, spearheaded by amazing post-doc @audreylumeau.bsky.social. Short thread to follow 🧬

NIGMS Advisory Council for May 22 has finally posted to the Federal Register. May the odds be ever in your favor, friends. www.federalregister.gov/documents/20...

FREEDOM OF EXPRESSION FOR HETEROCHROMATIN

Very interesting!

How do replisomes walk on DNA? And what happens when they run into a G-quadruplex? @sahilbatra.bsky.social and @benallwein.bsky.social provide unexpected insight in our latest paper with Richard Hite @mskcancercenter.bsky.social @science.org www.science.org/doi/10.1126/.... Congrats to all authors!

1/46 Hey folks, we have a new paper out on the MuLTEE. Strap in and I’ll tell you the story of how this “little paper on polyploidy” turned into the most data rich paper our lab has produced, largely thanks to the leadership and work ethic of @kaitong25.bsky.social. www.nature.com/articles/s41...

Local traditional media still works! We shot this interview on the grounds of the former J&L steel mill, where buildings including my lab are today. The threat to biomedical research in Pittsburgh is on par with the threat to the mills 50 years ago. www.wtae.com/article/pitt...

Beautiful work by the Lemmens lab! www.biorxiv.org/content/10.1...

Dear manager: 1: I try to say goodbye 2: I choke 3: I try to walk away 4: I stumble 5: I try to hide it, it's clear My world crumbles BECAUSE YOU IDIOTS ARE DESTROYING THE NIH

Nature research paper: Mechanism for local attenuation of DNA replication at double-strand breaks https://go.nature.com/4k8AdCX

This is the first movement from Sen. Fetterman, please fill out immediately if you have been impacted. Please circulate Penn, Pitt, PSU, Temple etc @cbartman.bsky.social

Does NOT apply to OGP and other Pitt School of Medicine PhD programs

How is it that I, a PhD program director at Pitt, heard about this from the media and not my University?!?

NIH indirects case update: Hearing ongoing now, and I'll try to live tweet some notable exchanges. Prior to more detailed arguments, Judge Kelley starts off with a few point questions to both sides: (I'm paraphrasing here)

𝐇𝐨𝐰 𝐝𝐨 𝐜𝐞𝐥𝐥𝐬 𝐫𝐞𝐦𝐞𝐦𝐛𝐞𝐫 𝐰𝐡𝐨 𝐭𝐡𝐞𝐲 𝐚𝐫𝐞 𝐚𝐟𝐭𝐞𝐫 𝐃𝐍𝐀 𝐫𝐞𝐩𝐥𝐢𝐜𝐚𝐭𝐢𝐨𝐧? Our new study “Disabling leading and lagging strand histone transmission results in parental histones loss and reduced cell plasticity and viability” is out in 𝘚𝘤𝘪𝘦𝘯𝘤𝘦 𝘈𝘥𝘷𝘢𝘯𝘤𝘦𝘴. Led by @lleonie.bsky.social @biranalva.bsky.social 🧵 More below👇

New #preprint from the lab! Do you want to see the invisible? So did we! When DNA genomes get broken, cells somehow find related sequences to fix the break. But how do they find it? We developed a way to look at sequence *search*, not just sequence usage. doi.org/10.1101/2025...

Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15) www.biorxiv.org/content/10.1...

Happy International Day of Women in Science. The National Science Foundation’s list of flagged words includes both “Women” and “Female.”

Judge last night temporarily blocked NIH indirect cost cuts in the lawsuit from AAMC and teaching hospitals. This applies US-wide www.documentcloud.org/documents/25...

Just got the dreaded email. University of Iowa is halting all NIH grant submissions. Losing a generation of scientists here will send the state backwards. What a travesty.

Doesn't apply to PA...