Luke Greenhough
@lukegreenhough.bsky.social
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🔬🥶 🧬
Excited to share our discovery of the RAD51 filament cap, built by a newly identified RAD51 paralog complex 🔬🧬
add a skeleton here at some point
about 2 months ago
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Luke Greenhough
MRC Laboratory of Molecular Biology
9 months ago
How do SMC complexes capture DNA? 🧪 Led by Frank Bürmann, Jan Löwe’s & Mark Dillingham’s groups have identified the directional loading mechanism the bacterial SMC complex MukBEF uses to capture & ingest DNA, & found a phage inhibitor to this pathway. Read more:
tinyurl.com/4s8nrrkk
#LMBResearch
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Luke Greenhough
Robert Appleby
9 months ago
Over five years of work has gone into our new preprint! The
#cryoEM
structure of a human RAD51 filament caught at the point of strand exchange 🧬
www.biorxiv.org/content/10.1...
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Structural mechanism of strand exchange by the RAD51 filament.
Homologous Recombination (HR) preserves genomic stability by repairing double-strand DNA breaks and ensuring efficient DNA replication. Central to HR is the strand-exchange reaction taking place within the three-stranded synapsis wherein a RAD51 nucleoprotein filament binds to a donor DNA. Here we present the cryoEM structure of a displacement loop of human RAD51 that captures the synaptic state when the filament has become tightly bound to the donor DNA. The structure elucidates the mechanism of strand exchange by RAD51, including the filament engagement with the donor DNA, the strand invasion and pairing with the complementary sequence of the donor DNA, the capture of the non-complementary strand and the polarity of the strand-exchange reaction. Our findings provide fundamental mechanistic insights into the biochemical reaction of eukaryotic HR. ### Competing Interest Statement The authors have declared no competing interest.
https://www.biorxiv.org/content/10.1101/2025.03.26.645421v1
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Luke Greenhough
Mekhail Lab at U. Toronto 🍁🇨🇦
10 months ago
🚀 New Paper Alert! Our latest
@natrevmcb.bsky.social
explores how nuclear and genome organization drive DNA double-strand break repair! 🧬 📖 Free access:
rdcu.be/edViW
Please 🔄 ❤️
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Nuclear and genome dynamics underlying DNA double-strand break repair
Nature Reviews Molecular Cell Biology - Changes in nuclear and genome organization promote the repair of DNA double-strand breaks and genome stability. Processes that are involved include the...
https://rdcu.be/edViW
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Luke Greenhough
Petr Cejka
9 months ago
Check out our review on DNA end resection! With Raphael Ceccaldi,
www.nature.com/articles/s41...
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Been excited for this for a while! Excited to chat about all things paralog related
add a skeleton here at some point
9 months ago
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Luke Greenhough
Andrew Deans
about 1 year ago
Excited to see the final published form of this amazing
@drewberry.bsky.social
animation of DNA repair go live! Watch your favourite friends BRCA1/2, RPA, RAD51, Exo1, BLM, PCNA, MRN, primase and DNA polymerase perform their recombination magic. Cool educational & scientific tool for our community.
add a skeleton here at some point
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Luke Greenhough
Ernst Schmid
10 months ago
Very excited to share that my thesis work is out in Molecular Cell! We trained a Structure and Omics informed Classifier (SPOC) to score binary AlphaFold multimer (AF-M) predictions by structural quality and consistency with experimental omics datasets.
www.cell.com/molecular-ce...
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Predictomes, a classifier-curated database of AlphaFold-modeled protein-protein interactions
Schmid and Walter train a classifier that discerns functionally relevant structure predictions in proteome-wide protein-protein interaction (PPI) screens using AlphaFold-Multimer, and they use this co...
https://www.cell.com/molecular-cell/fulltext/S1097-2765(25)00105-4
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Luke Greenhough
Tony Cesare
11 months ago
Thank you to Roger Greenberg and Hilda Pickett for organising a fantastic 2025 Mammalian DNA Repair GRC in Ventura, California. Looking forward to the next time.
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