@science.org Evolution of error correction through a need for speed | Science #evolution 🧬🔬 www.science.org/doi/10.1126/...

New pre-print from the lab! Lead by Mariia Mikhova and in collaboration with Kefei Yu’s lab @msumgi.bsky.social we dug into the molecular mechanism of class switch recombination in B cells using simultaneous RNA and protein single-molecule imaging. 1/n www.biorxiv.org/content/10.6...

New @natcomms.nature.com paper today from Tracy Bryan's lab @cmri.bsky.social: "Nuclear actin and DNA replication stress regulate telomere maintenance by telomerase". Happy our lab could play a small part. Congrats Tracy, Ash Harman, Noa Lamm and the whole team. www.nature.com/articles/s41...

New lab preprint! ERCC6L2 disease is a recessive bone marrow failure syndrome caused by mutations in the putative DNA helicase ERCC6L2. Using mouse genetics, biochemistry and AF3 we uncover ERCC6L2-MRI as a KU-regulatory complex stimulating NHEJ at staggered DSBs: www.biorxiv.org/content/10.1...

New Print Alert! A fun collab with Kathy Meek at MSU. Led by talented bioinformatician Giovanni Pascarella we demonstrate that Ku70/80 binds intronic antisense Alu elements to inhibit cryptic splice sites. For details see the thread below! 1/n www.biorxiv.org/content/10.1...

Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...

"Our findings uncovered a species survival strategy in which telomerase RNA extends telomeres for the next generation by hitchhiking on introns of germline-expressed genes." 🤯 #Celegans 🧪

1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria. www.biorxiv.org/content/10.1...

🚨 Excited to share my main postdoc work from the @jenscs83.bsky.social lab, now out in Cell Reports! 👉 www.sciencedirect.com/science/arti... We dissect how shelterin proteins organize at telomeres in human cancer cells—revealing distinct subcomplexes that maintain chromosome ends. 🧵

Find the plasmids here! www.addgene.org/Francesca_Bo... Thanks for sharing protocols and materials!

Our protocol for gene editing is online and all plasmids are on @addgene.bsky.social! CRISPR away everybody and get in touch if you need anything 🤩

New preprint! We solve a mystery you didn't know existed. Mitotic cells lack new transcription but require ongoing translation. Interphase mRNA half life is only 2-4 hrs. So how do cells arrest in mitosis for hours without depleting their transcriptomes? www.biorxiv.org/content/10.1...

Excited this study is out - we helped with an experiment on Paxx www.nature.com/articles/s41...

New pre-print! Ryan Kim et al distinguish the roles of the Spindle Assembly Checkpoint as a surveillance pathway only triggered by mitotic errors vs a mitotic timer that is required in every cell division. www.biorxiv.org/content/10.1...

The 3rd Berlin Single Molecule Biophysics Course will run from 8th to 12th September at the HU-Berlin, Institute of Biology. Learn about single channels, single molecule TIRF, theory and analysis. Apply by 7th July at [email protected]. Fee waivers and bursaries available. ! Please share widely !

Are you looking for a faculty job? We are hiring in my department at Roswell Park NOW. Contact me for details as I await the formal ad. (Please RT.)

🧪 #neurology #neuroskyence tinyurl.com/bp5d8386 Another beautiful example of NIH-SPONSORED bedside to bench and back to bedside work from an outstanding clinician scientist. This work is producing some of the most compelling responses to treatment in what is arguably the worst pediatric brain tumor.

New preprint from the lab @kaelamakins.bsky.social Genetic interactions between 53BP1-RIF1 and DNA-PKcs for DSB repair outcomes. www.biorxiv.org/content/10.1...

A great end to another Telomeres CSH meeting! It was nice to see and discuss science with many colleagues. With @cijilim.bsky.social @jenscs83.bsky.social, Stone, Nandakumar, Zappulla labs at banquet

🧵1/ 🚨Check out our significantly updated preprint, now out on @biorxivpreprint.bsky.social. Together with Sasi Conte lab, we characterize a novel role for intrinsically disordered regions (IDRs) in promoting RNA-binding selectivity in RNA binding proteins (RBPs): www.biorxiv.org/content/10.1...

And on other news. My first contribution to science as a postdoc with @stirlingchurchman.bsky.social is out! We discuss the knowns and unknowns of post-transcriptional splicing. It was really fun to write with the great @karinechoquet

Telomere Community 🧬 Can't wait for the new CSHL Monograph? Our review on unusual structure at Telomeric repeats is now live on Cold Spring Harbor Perspectives! Grab a few free PDFs here: cshperspectives.cshlp.org/cgi/reprint/... #CHSL #AcademicSky #Science @ezeta.bsky.social @cshlnews.bsky.social

Happy to share our latest pre-print! We solved the cryoEM structures of human DNA repair factor MRN bound to DNA and to TRF2. Main work of Yilan, Filiz @filizkuybu.bsky.social and Hengjun. Great collab within the @sfb1361.bsky.social and @genecenter-lmu.bsky.social 🧬🔨 www.biorxiv.org/content/10.1...

Plasmids for our destabilized MCP (dMCP) & PCP (dPCP) coat proteins are now on @addgene.bsky.social here: www.addgene.org/browse/article/28252367/ These proteins are degraded by cells except when bound to their RNA hairpin targets (MS2 & PP7): www.biorxiv.org/content/10.1101/2024.11.21.624393v1

Cash lab is hiring a junior specialist/lab manager. Please feel free to reach out to me for details! Link to apply here: recruit.ucdavis.edu/JPF07011 Lab website here: cash4rhogefs.faculty.ucdavis.edu

🥳Just published @nature.com. Over 25 years after the discovery of SPO11 as the enzyme responsible for initiating meiotic recombination, we finally succeeded in reconstituting its DNA cleavage activity.🧵 www.nature.com/articles/s41...

Thanks @franbottanelli.bsky.social for leading this effort! Addition genome editing plasmids are available on our labs addgene page. www.addgene.org/Jens_Schmidt/

Long in the making but our protocol for gene editing is online! FAB CRISPR is super easy and allows the generation of N and C terminal fusions with TurboID, SNAP, Halo and mStayGold. A single cloninf step to generate HDR templates! arxiv.org/abs/2502.12675 1/n

I maxed out my credit card, emptied my savings account, and took out a loan to move from Alabama to Bethesda, MD. I don’t even qualify for unemployment since I’ve only worked at NIH for a month. I will be financially and medically devastated. Seeking suggestions for anywhere that’s hiring!!

Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15) www.biorxiv.org/content/10.1...

In rare good news these days Hängen-Dasz has brought back pralines and cream! Even better, it was on sale!

Excited to share James Jusuf's preprint: By integrating Micro-C with SuperRes Live-Imaging we can calibrate genomics&imaging to perform absolute quantification of looping (e.g. this loop is present 3%) We quantify mESC 36k loops: <loops> are generally rare (2.3%) www.biorxiv.org/content/10.1...

We used cryoET and biophysical approaches in vitro together with molecular biology in differentiated cells to show that chromatin compacted by PRC1-CBX8 is porous and accessible while PRC1 is dynamic in it. 🧪🧬 #cryoET #Epigenetics #Polycomb www.nature.com/articles/s41...

New preprint from the lab! We defined the kinetics of strand invasion during meiosis in C. elegans. Great work from Antonia, Henry and Divya. www.biorxiv.org/content/10.1...

Building on our discovery of #LesionSegregation, @mikespencerchapman.bsky.social and colleagues from @sangerinstitute.bsky.social find that some DNA lesions can persist in humans for months or years! "Prolonged persistence of mutagenic DNA lesions in somatic cells" www.nature.com/articles/s41...

The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain https://www.biorxiv.org/content/10.1101/2025.01.10.632395v1

Super excited to share our most recent pre-print, lead by K99 fellow @heyzajr.bsky.social, who will be starting his own lab at Wayne State University in February! The question is how are DNA breaks held together during HR?? 1/n www.biorxiv.org/content/10.1...

1/Delighted to announce the newest paper from our lab, “Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage”, is out today in @naturecellbiology.bsky.social, www.nature.com/articles/s41....

Here is a fun little teaser! What DNA repair factor does this to mitotic chromosomes? Pre-print should be up any time now! Exciting work by @heyzajr.bsky.social!

New preprint w/ surprising insights into proteasomes + mitosis. Océane Marescal found strikingly different cellular phenotypes for proteasome subunit knockouts. 19S lid depletion results in ubiquitin-INDEPENDENT degradation of the motor KIF11 + monopolar spindles. www.biorxiv.org/content/10.1...

Nice punt Goff!

Really cool work, demonstrating TRF2-RAP1 mediated inhibition of the NHEJ machinery at chromosome ends! www.biorxiv.org/content/10.1...

Woohoo, we are live! Check out our manuscript describing our discovery of PRR12 and its role in cohesin regulation: authors.elsevier.com/a/1kMQf5Sx5g.... I am excited for my lab to follow up on this work and uncover the mechanisms by which PRR12 (& other regulators) contribute to genomic integrity!

Nice thread on our Christmas pre-print to those who celebrate telomere biology!

Check out our newest pre print led by post doc extraordinaire @janovictom.bsky.social. Thread with more details coming tomorrow!

All I want for Christmas is our pre-print to be screened!

I am just here to see @mikefeigin.bsky.social freak out about the Bills!

Heading to Costco!! I will see you on the other side.

Hi Bluesky community! 👋 Are you looking forward to 2025 as much as we are? 👀 We just updated our 2025 Annual Poster to include even more events for you to join, so go and have a look! ➡️ s.embl.org/poster-bl #EMBLEvents #molecularbiology #lifesciencetraining