New pre-print from the lab! Lead by Mariia Mikhova and in collaboration with Kefei Yu’s lab @msumgi.bsky.social we dug into the molecular mechanism of class switch recombination in B cells using simultaneous RNA and protein single-molecule imaging. 1/n www.biorxiv.org/content/10.6...

First test of live-cell single-molecule imaging in a new lab and it looks very promising!!! Thanks to @ceitec-cellimcf.bsky.social instruments at @ceitec.eu and @masarykuniversity.bsky.social. 🙌 #microscopy #singlemolecule #livecell #newlab

What is actually a high-risk, high-gain project proposal? Great to hear the answer directly from the ERC president Maria Leptin. 🎧 nightscience.buzzsprout.com/1744020/epis... Night Science (@nightsciencepod.bsky.social) is simply an amazing podcast for scientists! #ERC #NightScience #GrantWriting

I’m excited to share that #CzechBioImaging selected my project for funding to establish live-cell single-molecule imaging at the @ceitec-cellimcf.bsky.social core facility at @ceitec.eu , @masarykuniversity.bsky.social ! Can’t wait to bring this technology to Brno! 🔬✨

Huge thanks to @masarykuniversity.bsky.social for organizing Grants Week! Hearing from holders of prestigious EU grants and learning from their experiences was very inspiring. www.grantsweek.muni.cz

New Print Alert! A fun collab with Kathy Meek at MSU. Led by talented bioinformatician Giovanni Pascarella we demonstrate that Ku70/80 binds intronic antisense Alu elements to inhibit cryptic splice sites. For details see the thread below! 1/n www.biorxiv.org/content/10.1...

IT'S HAPPENING! 💥 I'm psyched to launch the collaboration between @qedscience.bsky.social & @openrxiv.bsky.social @biorxivpreprint.bsky.social! Preprint + q.e.d = your science is out there, and anyone can appreciate it. Let's care about making discoveries, and not on “getting published” (1/3) 👇

Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...

1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria. www.biorxiv.org/content/10.1...

Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1... Read the thread below for a summary of our findings 🧵1/11

🚨 Excited to share my main postdoc work from the @jenscs83.bsky.social lab, now out in Cell Reports! 👉 www.sciencedirect.com/science/arti... We dissect how shelterin proteins organize at telomeres in human cancer cells—revealing distinct subcomplexes that maintain chromosome ends. 🧵

Finally out in Nature Chem Bio: SNAP-tag2 for faster and brighter protein labeling www.nature.com/articles/s41... Thank you Steffi and Veselin.

Great new DNA/Q-Paint method published by our @ucl colleague Sabrina Simoncelli - lucky to have been part of it! Check out the Ecadherin and collagenIV imaging in the fly retina at up to 15micron depth www.nature.com/articles/s41...

Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!! www.nature.com/articles/s41... @spirochrome.com

The ERC welcomes the offer of substantial additional budget from @ec.europa.eu for the development of a new ERC funding instrument offering larger, longer-term grants, as announced by President Ursula Von der Leyen at the Sorbonne this morning. 👉 europa.eu/!NTYTTV

A great end to another Telomeres CSH meeting! It was nice to see and discuss science with many colleagues. With @cijilim.bsky.social @jenscs83.bsky.social, Stone, Nandakumar, Zappulla labs at banquet

🚨🔬💗Whether investigating cell organelles or mapping proteins, together with Victor Puelles's lab we lay a roadmap for selecting optimal #ExM and #SuperResolution #microscopy combinations. Daria Aristova and Dominik Kylies review with amazing co-authors pubs.aip.org/aip/apr/arti...

Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social, @marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social 🔗 Preprint here tinyurl.com/3a74uanv

Our new findings on how chromosomes get ready for cell division are now published in @cellpress.bsky.social! Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩 www.sciencedirect.com/science/arti...

1/Out today in @naturecomms.bsky.social, “A CPC-shelterin-BTR axis regulates mitotic telomere deprotection”. Here we identify the mechanism that unwinds telomere-loops (t-loops) during mitotic arrest to activate the DNA damage response and signal mitotic stress. www.nature.com/articles/s41....

#Hiring! The postdoctoral position in my lab at Pharm Chem KU is open!! This is an NIH-funded position to work on cytochrome P450 metabolism using a combination of mass spectrometry proteomics and live-cell imaging. employment.ku.edu/staff/29681BR

Super excited to share our most recent pre-print, lead by K99 fellow @heyzajr.bsky.social, who will be starting his own lab at Wayne State University in February! The question is how are DNA breaks held together during HR?? 1/n www.biorxiv.org/content/10.1...

1/Delighted to announce the newest paper from our lab, “Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage”, is out today in @naturecellbiology.bsky.social, www.nature.com/articles/s41....

We made a photoswitchable HaloTag (psHaloTag), which can reversibly turn-on fluorogenic dyes upon illumination 💡. Congrats to Franzi, Bego and all co-authors, check out our preprint below 👇 www.biorxiv.org/content/10.1...

1/8 Super excited to share my first-author preprint in @jenscs83.bsky.social lab! We measured the shelterin complex stoichiometry and dynamics at telomeres in living cells. Our results re-envisions the architecture of telomeres in human cancer cells. www.biorxiv.org/content/10.1...

Our paper of today is the result of the creative hard work of first authors Richard Janissen and @romanbarth.bsky.social in our #CDlab, in collaboration with Iain Davidson and Jan-Michael Peters of the IMP Vienna BioCenter. Enjoy reading our paper here: www.science.org/doi/10.1126/... 10/10

🔺🔺🔺RED TRIANGLE ALERT 🔺🔺🔺 Ever wonder how #TADs compare across the tree of life?Look no further & read our Review!!! Find out what genes & 3D chromatin can & can't do in Bacteria! Archeae! Yeast! Plants! Animals! SMCs & RNA-Pol are the only thing they have in common www.nature.com/articles/s41...

“In this Review, we discuss #telomere protection, maintenance and damage. We highlight the differences between #human and #mouse telomere biology that may contribute to discrepancies between human #diseases and mouse models.” #telomerase #aging www.nature.com/articles/s41...

Super excited to share our latest paper led by graduate student Mariia Mikhova! She used single molecule live cell imaging to dissect the kinetics of DNA break repair by non homologous end joining. We estimate the NHEJ pathway can repair up to 1000 breaks per minute! www.nature.com/articles/s41...

First attempt in a (loosely defined) Single-Molecule Biophysics starter pack. Please share and (self-) nominate. go.bsky.app/CteFZM5