Structures redefine the mechanism of action for tetracyclines https://www.biorxiv.org/content/10.1101/2025.09.25.678686v1

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

Check out our new paper: a review of translational coupling, the phenomenon where translation of one prokaryotic gene can promote translation of the gene downstream. We cover the history, and delve into the mechanism, which is still not fully understood. journals.asm.org/doi/10.1128/...

Mechanism of 30S subunit recognition and modification by the conserved bacterial ribosomal RNA methyltransferase RsmI #microsky #rnasky 🦠 www.biorxiv.org/content/10.1...

Structural insights into YheS-mediated release of SecM-arrested ribosome. https://www.biorxiv.org/content/10.1101/2025.08.28.672766v1

OMG this is it?! The final piece in the puzzle?!😲 tRNAs are the key, because archaic tRNAs sit at the core of all ribosomes. If aminoacyl-tRNAs could form on early Earth, you have a plausible route for the first self-replicators, the bridge from chemistry to biology www.nature.com/articles/d41...

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Happy to share a new paper from our lab with my little contribution! 🥳🥳🥳 We show that antibiotic resistance ABC-F proteins fine-tune responses to ribosome-binding antibiotics. Thanks to ABC-Fs, bacteria can differentiate between antibiotic classes and even concentrations for a tailored response. 🦠💊⬇️

Finally out! I’m thrilled to share our new paper (Wolin et al., Cell 2025). This paper describes SPIDR, a high-throughput method for mapping RBP binding sites. By combining #SPIDR with #cryoEM, we identified the exact binding site of LARP1 within the #mRNA channel of the 40S ribosomal subunit.

Out now: Structure of an archaeal ribosome reveals a divergent active site and hibernation factor @jhdcate.bsky.social @banfieldlab.bsky.social @diptinayak.bsky.social & co #microsky www.nature.com/articles/s41...

😲 E-site binding antibiotic discovered 👏👏

New paper out in Mol Cell, where we show how N-myristolytransferases lipidate proteins on the translating ribosome! Continuing our successful collaboration with DeuerlingLab @uni-konstanz.de & Shu-ou Chan @caltech.edu @snsf.ch @ethz.ch www.cell.com/molecular-ce...

Structural studies on ribosomes of differentially macrolide-resistant Staphylococcus aureus strains pubmed.ncbi.nlm.nih.gov/40490363/ #cryoem

In situ protein crystallography: a single-crystal electron diffraction pipeline for structure determination inside living cells. www.biorxiv.org/content/10.1101/2025.07.08.663504v1 #cryoem

Insights on how ribosomal RNA modification distant from drug binding site can still affect drug binding, in mycobacterium 🦠 #rnasky #ribosome #microsky

Happy to say that my Current Opinion in Structural Biology article came out today. I hope it helps to guide to you in using cryo-ET to make new biological discoveries! www.sciencedirect.com/science/arti...

Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...

Beautiful paper on translation start sites and extented SD motifs in Staph. aureus. www.biorxiv.org/content/10.1... Congrats to colleagues in Strasburg univ.!

@science.org Mechanism of release factor–mediated peptidyl-tRNA hydrolysis on the ribosome | Science www.science.org/doi/10.1126/... #ribosome #RNA #hydrolysis

Mechanism of release factor–mediated peptidyl-tRNA hydrolysis on the ribosome www.science.org/doi/10.1126/...

Proteins operate on a short timescale — on the order of a millisecond, or even faster https://go.nature.com/4l7qAEm

I’m thrilled to share my very first first-author preprint and our lab’s latest work💫 We discovered a novel role for eIF2A in ribosome-associated quality control (RQC), challenging decades-old assumptions about its enigmatic function in translation initiation. Check it out to learn more! ⭐️ #RNA

New paper from the lab! Want to know if there is a difference in ribosome collisions between your samples? We show that you can detect this in the rRNA reads in your ribosome profiling data. academic.oup.com/nargab/artic...

In our newly published study we show that the proline-rich antimicrobial peptide (PrAMP) Bac5 has a distinct mode of interaction with the ribosome compared to other PrAMPs…https://academic.oup.com/nar/article/53/8/gkaf324/8126264

You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software! Hope it will be useful !

Ribosome collisions and stalling senses m6A presence in mRNA which then starts degradation of m6A-containing mRNA. www.cell.com/cell/abstrac...

tRNA modifications tune m6A-dependent mRNA decay: Cell www.cell.com/cell/fulltex...

Small RNA pairing in coding sequences can locally slow translation elongation and impact cotranslational protein folding and activity #microsky #rnasky 🦠 www.cell.com/molecular-ce...

tRNAs are small and sport heterogeneous base modifications – yet @sebastianglatt.bsky.social @jmbujnicki.bsky.social Ting-Yu Lin 林亭妤 et al show #cryoEM combined with biophysical & computational approaches can give insights into how #pseudouridine affects human tRNA! www.embopress.org/doi/full/10....

A µORF controls translation (and transcription) of the type II topAI/yjhQ toxin antitoxin module which is induced under various ribosome-targeting ATB! elifesciences.org/reviewed-pre...

Happy to share our recent work on rRNA processing and conformation: www.biorxiv.org/content/10.1... Huge thanks to @ppenev.bsky.social @Amos Nissley @Dipti Nayak @Rohan Sachdeva @jhdcate.bsky.social @Jill Banfield @banfieldlab.bsky.social !!

1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.

Latest work from the lab - we identify a translation factor, YebC2, that reduces ribosome stalling in B. subtilis! Congrats to co-first authors: grad students Hye-Rim Hong & Cassidy Prince & undergrad Dennis Wu @denniswu02.bsky.social journals.plos.org/plosgenetics...

Preprint! All-atom structures of 2 folding intermediates on the ribosome, along parallel pathways & conserved across Ig domains, by 19F NMR & MD Co-led by @julianstreit.bsky.social, & thanks twlodarski.bsky.social, Alki, Lisa & John Christodoulou! #nmrchat #compbio www.biorxiv.org/content/10.1...

The XXI Discussions in Structural Molecular Biology & 8th #CIISB User Meeting 📢 27 talks, 9 flash talks, 51 posters networking🗣️ 🏆 Student award winners (oral presentation) Michaela Novotná |Antibiotic Resistance and Microbial Metabolomics Lab at IMIC👏 Klára Kohoutová @sciencecharles.bsky.social

Cryo-EM, and integration with MD simulations, of nascent proteins on the ribosome. Congrats Alki and @twlodarski.bsky.social and co-authors!

Mass spectrometrist looking for a postdoc? Why not in Switzerland 🇨🇭 We are reinforcing our team in Geneva ⤵️ www.linkedin.com/posts/valeri...

Can expression of a peptide lead to antimicrobial resistance? Yes it can! We discovered this for a toxin we work with and started exploring how #peptides, #antimicrobialresistance and de-novo gene birth can come together. @cp-trendsmicrobiol.bsky.social Amazing work by @rianneprins.bsky.social

I am thrilled to share this new preprint from our lab! We find (>800!) 30S binding sites in hundreds of 5'UTRs, but also new dynamic steps of translation initiation. www.biorxiv.org/content/10.1... Congrats to all co-authors at the RNA control of gene expression lab, IBPC CNRS (Thread, 1/6)

A new publication from my lab a great work done with fantastic colleagues from #IGDR (Reynald Gillet’s team for all the cryoem work. pubmed.ncbi.nlm.nih.gov/40187343/

doi.org/10.1515/hsz-... I am super happy to share this review as my first post on Bluesky. After submitting my PhD thesis at the start of the year, my PI Elke Deuerling suggested to write this review with her, covering many of the findings of my thesis. I am excited to see it published today!

Joe et al. have a fresh preprint on the MARTINI simulations of the #ribosome. arxiv.org/abs/2503.21357

New antibiotic that kills drug-resistant bacteria discovered in technician’s garden The molecule targets bacteria in a way that other drugs don’t. www.nature.com/artic... 1/3

🚨New paper!🚨 A comprehensive take on the origins and evolution of translation factors & how these essential players evolved across the tree of life. 🌍🧬 Led by Evrim Fer @uwmadisonmdtp.bsky.social grad student! 👏 Free access: www.sciencedirect.com/science/arti... @cp-trendsgenetics.bsky.social

The next big advance since the protein prediction revolution? VantAI has just announced Neo-1. It unifies structure prediction with all-atom de novo generation. Neo-1 excels in predicting ternary complex structures. It also integrates with experimental data for rapid iterative optimization.

Huge thanks, I feel very honored, and congratulations to the other winners! Publication will hopefully follow soon, stay tuned! 🤘 Presented work done with @balikovalab.bsky.social & @innislab.bsky.social