#microsky Integrated Tn-seq and MAGE-assisted rapid genome engineering targeting in Escherichia coli: Trends in Biotechnology www.cell.com/trends/biote...

Our new paper maps the tRNA modification landscape in Vibrio cholerae! 💫 We describe differences from E. coli and discuss links to decoding of stress-related codons 🦠 Huge thanks to amazing co-authors and collaborators! @plos.org #rnasky #microsky #tRNAmodifications

Phosphorylation of a conserved intrinsically disordered region is necessary for activation of a bacterial Hanks-type Ser/Thr kinase signaling pathway https://www.biorxiv.org/content/10.1101/2025.11.01.686061v1

Split-luciferin Assay for Real-time Measurement of Cytosolic Drug Accumulation in Live Mycobacteria https://www.biorxiv.org/content/10.1101/2025.11.02.686125v1

#synbiosky #microsky www.sciencedirect.com/science/arti...

🧪🔬🦠🧫🌍🪲🐛 A membrane network of the nutritional symbiont 𝑆𝑜𝑑𝑎𝑙𝑖𝑠 𝑝𝑖𝑒𝑟𝑎𝑛𝑡𝑜𝑛𝑖𝑢𝑠 woven with the cytosol of its host, the cereal-eating curculionid 𝑆𝑖𝑡𝑜𝑝ℎ𝑖𝑙𝑢𝑠 spp., to increase carbohydrate supply to the bacteria! Crazy amazing methods with amazing pics and vids! #BugSky #MicroSky #HostMicrobiome

Applications closing tonight (midnight French time) !!! Still a few hours to apply !!

In @nature.com: Using AI and other cutting-edge techniques, the Klinge lab has captured the first near-continuous "molecular movie" of ribosome formation—revealing, frame by frame, how cells build the protein factories that make life possible. More here: https://bit.ly/3LbHTaF

Honored to be part of a collaborative team receiving funding to genetically engineer methanogens in ruminants for reducing carbon emissions. We're now seeking multiple postdocs in the USA, Germany, and New Zealand - apply via the link! docs.google.com/forms/d/e/1F... 🧫🧪 #ArchaeaSky #MicrobiomeSky

Multiple small, curved, predatory bacteria (Bdellovibrio bacteriovorus) lyse the bacterial prey remnants below. How are they doing this & which predator proteins are involved in this process? See our newly released research article 📑 => rdcu.be/eL6gu 🦠 #microsky #bacteria #PredatoryBacteria

academic.oup.com/nar/advance-... #microsky a database of bacterial small RNA experimental data

De-DUFing the DUFs 🧩 @franznarberhaus.bsky.social lab uncovers how small DUF1127 proteins regulate #phosphate uptake by binding the sensor kinase PhoR. Their conserved role from Agrobacterium to E. coli highlights how even small DUFs can shape bacterial physiology 🦠 buff.ly/jJd9Eho

who would have thought! phage N15 TelN telomerase has more than one job! (and no, it's not 'moonlighting') great work from the gruber lab 👏 #MicroSky + #PhageSky

If you are a USA or Canada citizen and want to join my lab for a postdoc on bacterial secretion systems, toxins, and/or MGEs, check out this opportunity and contact me. 🦠 Zuckerman STEM Postdoc Program: Research in Israel for US & Canadian Scholars share.google/YOOdDRvUgGm1...

OUT NOW: Inducible transposon mutagenesis identifies bacterial fitness determinants during infection in mice #microsky 🧪 www.nature.com/articles/s41...

#microsky 😮😮

www.pnas.org/doi/10.1073/... #phagesky #phage defence #synbio #microsky

SPT of DNA repair proteins after cell irradiation by Ashli Kobayashi @asaki-kobayashi.bsky.social and Romane Riou. Setup by Abbelight @abbelight.bsky.social and irradiator by Errol Laser @errol-laser.bsky.social. Look at that recruitment! #Mifobio2025 @gdrimabio.bsky.social

1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria. www.biorxiv.org/content/10.1...

Ribosome hibernation in archea #microsky #ribosome 💫 www.biorxiv.org/content/10.1...

And it's out! Our paper on the optimization of smFISH to quantify cytokine mRNAs in T cells is published in EMBO Journal. Using this protocol, we gathered interesting insights on cytokine mRNAs co-expression and localization in these teeny tiny human cells. www.embopress.org/doi/full/10....

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Epitranscriptomic control of stress adaptations in Escherichia coli https://www.biorxiv.org/content/10.1101/2025.10.14.682255v1

μGrowthDB: querying, visualizing, and sharing microbial growth curve data https://www.biorxiv.org/content/10.1101/2025.10.13.682118v1

Great study reshaping how we think about TF–DNA binding - super relevant to our bacterial TF-DNA prediction model! 🧬🧪 🦠 DNA flanking high-affinity binding sites form overlapping lower-affinity binding sites that together modulate binding. #microsky

New on Think Like a Microbe ... Iron! 🧫🧪 #philsci open.substack.com/pub/thinkmic...

#microsky 😮

A new #CryoET study shows that the protein ZapD helps organise FtsZ filaments into stable ring-like structures in building bacterial division machinery. buff.ly/saLPqJM

@lcbofficiel.bsky.social @cnrsbiologie.bsky.social @univ-amu.fr

Ciprofloxacin-based ionic liquids demonstrate increased mutation frequency in Escherichia coli https://www.biorxiv.org/content/10.1101/2025.09.29.679178v1

Droplet-based Random Barcode Transposon-site Sequencing (Droplet RB-TnSeq) to characterize phage-host interactions https://www.biorxiv.org/content/10.1101/2025.09.29.679331v1

Precisely calling mutations across hundreds of bacterial isolates has been hard, requiring manual filtering and expertise. Until now, using AccuSNV. Herui Liao trained an ML model based on our previous meticulously called SNVs. www.biorxiv.org/content/10.1...

Check out the first preprint from our lab describing a direct molecular cross-talk between (p)ppGpp and c-di-GMP nucleotide messengers! Great work from first-author Corentin Jaboulay who was a postdoc in our lab, and great collaborations! @mmsb-lyon.bsky.social @cnrsbiologie.bsky.social

''including ORFs within tRNA loci.'' 🤷🏻‍♀️🧐 study detects significant ORF hits at 10 distinct tRNA loci under high chloramphenicol selection 🦠

Dual transposon sequencing profiles the genetic interaction landscape in bacteria | Science www.science.org/doi/10.1126/...

Cold shock adaptation and ribosomal RNA modifications 🦠#rnasky www.biorxiv.org/content/10.1...

#microsky #synbiosky Non-model bacteria as platforms for endogenous gene expression in synthetic biology www.nature.com/articles/s44...

📝New preprint! #MicroSky 🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy. 🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation. www.biorxiv.org/content/10.1...

Type 6 secretion system immunity protein RhsFI has been repurposed for small RNA regulation in pathogenic Escherichia coli academic.oup.com/nar/article/...

Using experimental evolution in host-mimicking media, researchers show that inflammation-like environments limit the loss of quorum sensing—a common adaptation during chronic infections—in Pseudomonas aeruginosa. Learn more in #mSystems: asm.social/2vd

🚀 Our new paper is out @natmethods.nature.com! Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells. 🔗 www.nature.com/articles/s41... 🧬 www.addgene.org/John_Ngo/

We discovered a new family of c-di-NMP receptors, widespread in bacteria & regulating🦠motility @pnas.org A great collab. w/ @mygalperin.bsky.social @vikramalva.bsky.social @thethormannden.bsky.social www.pnas.org/doi/10.1073/... @hhu.de @cmfi.bsky.social @sfb1381.bsky.social @mibinet.bsky.social

Guess it’s time to shake up course material!

Call for a tenure-track position at our Department of Plant Molecular Biology in Lausanne! We are searching for promising early-career researchers in the broad field of plant-organismal interactions. Deadline: November 30, 2025 - Please re-post! career5.successfactors.eu/career?caree...

Recent study in @natbiotech.nature.com: A team led by #HIRI’s Beisel lab introduced a new way to fine-tune genetic material. They describe an innovative technique where chemical tags are attached directly to DNA, opening new possibilities in medicine, agriculture, and biotechnology. ➡️ rdcu.be/eG4fk

What controls how/when growing RNA unblocks the "door" to exit RNA polymerase? Brilliant duo Anna Wang and Abhishek Majumder led a study in my lab - now out in NAR - on this. *Sequence* matters a lot! Read on... Super smFRET/dynamics work as well. Excited! academic.oup.com/nar/article/...

Bacterial two-hybrid systems evolved: innovations for protein-protein interaction research #MicroSky 🦠