New preprint with Benjamin Lang, Richard Kriwacki, John Christodoulou, and M. Madan Babu! www.biorxiv.org/content/10.1... Protein Dynamics at Different Timescales Unlock Access to Hidden Post-Translational Modification Sites #bioinformatics #compchem #folding #proteindynamics

The third episode of The Tortured Proteins Department is out now! We chatted about grant cancellations, exciting regional meetings and reunions, two fun new preprints, community norms around code release, and the importance of giving kudos. @fraserlab.com

Rationally designing 19F probe pairs was key to determining the structures of protein folding intermediates on the ribosome in our latest preprint. Our design strategy is now published in @natcomms.nature.com www.nature.com/articles/s41... #NMRchat #compbio #compchem

Preprint! All-atom structures of 2 folding intermediates on the ribosome, along parallel pathways & conserved across Ig domains, by 19F NMR & MD Co-led by @julianstreit.bsky.social, & thanks twlodarski.bsky.social, Alki, Lisa & John Christodoulou! #nmrchat #compbio www.biorxiv.org/content/10.1...

Cryo-EM, and integration with MD simulations, of nascent proteins on the ribosome. Congrats Alki and @twlodarski.bsky.social and co-authors!

Preprint! The ribosome’s electro charge defines how proteins fold by all-atom MD and 19F NMR. Led by @julianstreit.bsky.social with @charles-burridge.bsky.social, Joel Wallace, @chriswaudby.bsky.social, Lisa Cabrita, John Christodoulou #nmrchat #compchem #compbio doi.org/10.1101/2025.02.10.637539

Check out our latest preprint on the role of the ribosome in the folding of its nascent polypeptides - an integrative study combining NMR experiments and atomistic simulations! #NMRchat #compchem #CompBio #ribosome #proteinfolding www.biorxiv.org/content/10.1...

The ribosome lowers the entropic penalty of protein folding

Rational design of 19F NMR labelling sites to probe protein structure and interactions https://www.biorxiv.org/content/10.1101/2024.12.11.627779v1

Out now! How to rationally design 19F labels to study structures of large biomol complexes, incl in cells. Using engineered ring currents, and designed with MD and AF. With co-first author Julian Streit, and Saifu Daya and John Christodoulou. #nmrchat #compchem www.biorxiv.org/content/10.1...