Happy Halloween everyone! 🎃

We are looking for a computational postdoc to work with us on new optimisation algorithms to make #RELION even better. Join our bubbly team at the @mrclmb.bsky.social in Cambridge, UK. 🤗 RTs appreciated. mrc.tal.net/vx/appcentre...

New lab preprint! @zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes 🧵 tinyurl.com/ukny4ptz

Excited to share our preprint on cytoskeletal organization in astrocytes! biorxiv.org/content/10.110… Postdoc @mewynne759 looked at microtubules, IFs/GFAP & actin along long elaborate processes. Wonderful to collaborate w postdoc @dharshinigopal & @NogalesLab on cryo-ET 🤓 1/x

HHMI adopts Plan U journals.plos.org/plosbiology/...

Yifan Cheng to Receive 2026 Anatrace Membrane Protein Award buff.ly/ooI1UDy

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️

Now published! Big congrats to first author @gginell.bsky.social We are actively working improving/updating various aspects of FINCHES; don't hesitate to reach out if you run into issues, have questions. www.science.org/doi/10.1126/...

The fantastically successful NIGMS MIRA R35 "expired" on May 17, after the most recent deadline--these removals are the most aggressive attack on American science in the current series of devastating actions 🧪

"to run a contest that you end up not funding as a private organization at this time is… both extremely wasteful of these people’s time, and just devastating in terms of morale." I spoke with @aniloza.bsky.social at STAT today about HHMI's decision not to fund this round of Hanna Gray applicants.

Alex Long, the last postdoc from my lab at Stanford, is starting her own lab, continuing her beautiful work on evolutionary cell biology using chytrid fungi as a model. Check it out! alonglab.org

Conference alert: Machines acting on DNA and RNA Madrid, May 28-30 Abstract deadline: April 24 Organised by @cniostopcancer.bsky.social. Come to share your research with experts in the molecular mechanisms of DNA & RNA machines. See the list of speakers below. Please repost.

Introducing blebbisomes! Cover art by @ento-sanchez.bsky.social! #CellBiology Article: www.nature.com/articles/s41...

More than 2 decades ago the PINK1 protein was found to be linked to Parkinson's disease, but its structure and how it was activated was not known. Until @science.org today. A triumph for cryo-EM and structural biology, introducing new targets for drug intervention. www.science.org/doi/10.1126/...

Another wonderful collaboration with @alanbrownhms.bsky.social lab 👇 Zhang lab (co-first author @qingweiniu.bsky.social) contribute the doublet structure from Crithidia fasciculata, a model organism that infects mosquitoes.

😳😱 R.I.P. Karen

Happy to share the beautiful structure of a Wadjet SMC complex by @roisnehamelinf.bsky.social, with support from the DCI and @drhonsworth.bsky.social ! This is a type II Wadjet with unique characteristics including a tandem KITE subunit, distinct coiled coil architecture and a deviant hinge.

Thrilled- the @bsdb.bsky.social medal in Wolpert's name marks 'vastly important contributions to research in our field, but also to the communication of its problems to a broader audience'. Communicating the importance of our research has never been more important when science is under such threat!

The @jcellsci.bsky.social has an upcoming special issue - Cilia and Flagella: from Basic Biology to Disease – guest edited by Pleasantine Mill (University of Edinburgh) and Lotte Pedersen (University of Copenhagen) . The submission deadline is 31 March. journals.biologists.com/jcs/pages/ci...

Some good news: The Federal Register has just posted NIH study sections, which are to meet in late March. NIH announces some key grant-review meetings will restart in late March | Science | AAAS www.science.org/content/arti...

If you've been calling Congress, keep calling & if not, start-we're getting their attention. We had a good visit to a Congressional office yesterday to discuss science funding & the staffer told us how busy they've been-usually the Senate shwitchboard gets 64 calls/minute-now they're getting 1600 🧪

Cryo-electron tomography of Tetrahymena #cilia by Sam Li, David Agard, Mark Winey provides insights into the intricate spatial organization of basal body inner junctions, compromised by loss of Poc1 www.embopress.org/doi/full/10....

People need to know that NIH grants are the backbone of American biology and thus of our biotech industry. I took a moment away from doing science to talk about how science gets done with @oaldridge.bsky.social with @kutnews.bsky.social, this time about recent IDC cuts. www.kut.org/science/2025...

Please see a Perspective we wrote on our recent findings: onlinelibrary.wiley.com/doi/full/10....

🧬🧪Protein codes promote selective subcellular compartmentalization | Science www.science.org/doi/10.1126/... Henry Kilgore @itamarchinn.bsky.social @pgmikhael.bsky.social Ilan Mitnikov, et al. @aihealthmit.bsky.social @whiteheadinstitute.bsky.social @mitofficial.bsky.social

Starting first posting in Bluesky! In this study, we delineate CatSper’s assembly order and molecularly define a binding surface for activity modulation, offering CatSper canopies as potential targets for fertility control. www.biorxiv.org/content/10.1...

Working on actin cytoskeleton and Arp2/3 complex? Check out our latest work～https://www.biorxiv.org/content/10.1101/2025.01.31.635869v1 In this work, we found SPIN90 forms a dimer, facilitating bidirectional actin filament nucleation by activating two Arp2/3 complex #Cryo-EM #Actin #Arp2/3

Check out our lab’s latest work on the regulation of transcription by RECQL5 helicase! This work was co-led by @alfredo0712.bsky.social and @nlue8.bsky.social, with contributions from Patricia Grob, Ben Kaeser, Jie Fang, and Susanne Kassube. 1/7 www.biorxiv.org/content/10.1...

Scientists in the @alushinlab.bsky.social have figured out how cells build a structure that lets them migrate. Unpacking these “finger-like” protein bundles that protrude from cells may lead to novel treatments for metastasis. #RockefellerScience bit.ly/3PKErmA

Hello #Bluesky! We'd like to introduce ourselves... We are the WashU Ciliopathy Research Group - a multidisciplinary team of investigators at Washington University in St Louis that perform fundamental, translational, and clinical research on #ciliopathies. Follow us for updates from the group! 👇

The Dept. of Biochem. & Biophys. @ WashU is running its summer undergrad research program! Rising seniors who have not had the opportunity to do bona fide research but are interested in this are strongly encouraged to apply Deadline: Jan 31st Details: biochem.wustl.edu/resources/in... Pls share!

The Cryo-EM: the next 50 years Symposium held yesterday at @ucberkeleyofficial.bsky.social was a smashing success!!

Work led by graduate student @trinitycookis.bsky.social and coauthors Alexandria Lydecker, @paulsauer.bsky.social, and @kasinath-lab.bsky.social is out today where we looked at the inhibition of PRC2 by histone PTMs associated with regions of active transcription. www.nature.com/articles/s41...

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍 Preprint📜: www.biorxiv.org/content/10.1... A short thread🧵👇

Our second attempt much improved and expanded, hopefully ready to submit! Different mechanisms link gain and loss of kinesin functions to axonal degeneration - functional link (DOI corrupt): www.biorxiv.org/content/10.1...

Best way to start 2025! Thrilled to share our Nature paper about the structures of axonemal components from sperm flagella and from epithelial cilia of the oviduct and brain ventricles. Great collaboration with Tzviya Zeev-Ben-Mordehai and @alanbrownhms.bsky.social www.nature.com/articles/s41...

Technically superb, live imaging experiments visualise intraflagellar transport in zebrafish ciliated tissues, unveil the surprising breadth of intraflagellar transport speed. https://buff.ly/41UJjMS

rdcu.be/dY5rl The latest research article from Ti Lab. We employed cryo-EM structural analysis, single-particle fluorescence microscopy, and recombinant tubulin to reveal how tubulin acetyltransferases modify the K40 residue inside the microtubule lumen.

#TeamTomo visits the centrosome. I remember the centrosome and PCM being one of Julia's first targets when we were postdocs together at @mpibiochem.bsky.social. It's cool seeing the progress here in worms with Jeff. Beautiful #CryoET across the cell cycle. @centriolelab.bsky.social take a look! 🧪🧶🧬

Cilia alert! Stoked for our new paper in Dev. Cell! Our cross-linking mass spec interactome for motile cilia provides some cool new insights into motile ciliopathy. Helmed by @computingcaitie.bsky.social, read her fantastic Bluetorial below (👇)! www.cell.com/developmenta...

Love the title and my goodness what a glycan tree 🍝

1/ A new series of #CryoSPARC tutorial videos from this year’s S2C2 #cryoEM image processing workshop are now online! These videos will be interesting to all users and especially those newer to #cryoEM. www.youtube.com/playlist?lis...

Online today @science.org Structural basis of H3K36 trimethylation by SETD2 during chromatin transcription by @jonmarkert.bsky.social @lucas.farnunglab.com et al. at @harvardcellbio.bsky.social #cryoEM #chromatin #transcription #cryoEM #Pol_II🧪🧬❄️🔬 www.science.org/doi/10.1126/...

Beautiful structure of apolipoprotein B100 🤩 www.nature.com/articles/s41... www.nature.com/articles/s41...

Our #CryoET paper on ciliary rootlet ultrastructure from mouse retina is now out in @elife.bsky.social! Have a peek with this video or dive deeper at bit.ly/3ZJzhgc. Big thanks to @carter-lab.bsky.social and #teamtomo. #CryoET #CryoEM #Cilia #Rootlet #Centriole #Research #Science

Proudly present our new preprint on my first post here. We performed SPA on the ciliary tip and identify 7 proteins. Among them, we found the conserved protein SPEF1 at the microtubule seam. In vitro assays show that SPEF1 binds to the seam and stablizes microtubules. www.biorxiv.org/content/10.1...

Online @cellpress.bsky.social, we reveal a protein shell that helps diatoms fix 20% of global #CO2 🧪🌊🌾🧶🧬🌍 Shimakawa et al: www.cell.com/cell/fulltex... Nam et al: www.cell.com/cell/fulltex... @biozentrum.bsky.social press release: www.biozentrum.unibas.ch/news/detail/... Read on for more... 1/🧵

🧪 Lab's latest!! Work by PD (and now PI) Alison Philbrook. C. elegans sensory neurons have gorgeous #cilia. We’ve always thought that a) no cilia = no sensory responses, & b) no IFT = no cilia. Punchline is we thought wrong. Read paper for more! #neuroscience journals.plos.org/plosbiology/...