Online now! CBX4 acetoacetylation as an inhibitory mechanism of HIF-1α activity #chembiol

Out in @natcomms.nature.com: Cryo-EM structure of the pseudo-HAT-containing O-GlcNAcase! Especially exciting since multiple companies are developing O-GlcNAcase inhibitors for Alzheimer's disease (tau is O-GlcNAcylated). Glad to have a small contribution in this story. Congrats to all authors!

Historically, viruses were thought to primarily use host cell's translational machinery. New work from @harvardcellbio.bsky.social faculty Amy Lee reveals that a giant DNA virus encodes its own IF4F initiation complex, suggesting an unexpected evolutionary innovation. www.biorxiv.org/content/10.1...

New work from our own Chouchani Lab finds that LRRC58 is the substrate adaptor of an E3 ubiquitin ligase that mediates proteasomal degradation of CDO1, the rate-limiting enzyme of the catabolic shunt of cysteine to taurine in response to altered Cysteine levels. www.nature.com/articles/s41...

Robert Redford (RIP) survived a childhood bout of polio though bedridden for 2 weeks In 2014, he directed a short film on the architecture and mission of La Jolla's Salk Institute—honoring founder Jonas Salk, inventor of the polio vaccine that has saved countless lives, and architect Louis Kahn

A few years back we discovered a dual hybrid protein modification composed of an ADP-ribose dinucleotide and the ubiquitin moieties (ADPr-Ub). Here you can read our review that will give you an update on this increasingly popular topic: rdcu.be/eETIT

Delighted to share our work on cellular ubiquitination of drug-like compounds by HUWE1 - a surprising journey! Kudos to all contributors & first authors, Barbara Orth and Pavel Pohl. Sincere thanks to @ireserra.bsky.social ‬ #NatCommun for expertly guiding the winding publishing path. rdcu.be/eDzPk

Are you a postdoc interested in the mechanisms of disease? Do you have a great record and an exciting vision? Come and start your own lab @dunnschool.bsky.social by applying for sponsorship for early career fellowships. Deadline 30th September...pass it on! www.path.ox.ac.uk/work-with-us...

Happy to share that my first-author paper from my postdoc at Imperial @imperialcollegeldn.bsky.social and The Francis Crick Institute @crick.ac.uk is now published in Cell Reports! www.sciencedirect.com/science/arti...

Want to know how lipidation of some nascent chains takes place by NMT2-- Check our latest work on how NAC couples Protein Synthesis with Nascent Polypeptide Myristoylation on the Ribosome out today @embojournal.org‬: www.embopress.org/doi/full/10....

Excited to share a new preprint from the lab. We show that PTMs like phosphorylation & glycosylation dynamically reshape proteome-wide ligandability in cells, including proteins like KRAS. Great collaboration with the Huang Lab, @forlilab.bsky.social and BMS. www.biorxiv.org/content/10.1...

I still can’t believe that all NIH grants to my colleagues at Harvard and Harvard Medical School have been nullified. And that, as a nation, we’re somehow okay with this illegal, arbitrary, and petty act. Just think of the consequences: scientists, students, and patients will all suffer.

A few years back we discovered a dual hybrid protein modification composed of ADP-ribose dinucleotide and ubiquitin (ADPr-Ub). Now we reveal that ADPr-Ub can be further ubiquitinated by the E3 ubiquitin ligase RNF114! www.nature.com/articles/s41... www.science.org/doi/10.1126/...

An amazing paper from van der Heden and Ahel groups! They use clever chemistry to identify RNF114 as a dual ADP-ribose-Ubiquitin reader involved in the DNA damage response! Congratulations to first authors Max, @chatrin-c.bsky.social and Rishov. Happy to have a small contribution in this story

This is wild! Engineering E. coli bacteria to turn plastic waste into paracetamol (Tylenol) www.nature.com/articles/s41... www.nature.com/articles/s41...

A few years back we discovered a novel dual hybrid protein modification composed of ADP-ribose dinucleotide and ubiquitin (ADPr-Ub): pubmed.ncbi.nlm.nih.gov/36197986/

New paper! We uncover mechanistic insights into the crosstalk between AHR (aryl hydrocarbon receptor, a transcription factor) and PARP7 (a nuclear enzyme that performs protein ADP-ribosylation). Both are promising cancer drug targets. Big thanks to all co-authors! www.pnas.org/doi/10.1073/...

We have another postdoc position available to work on interplay between ADP-ribosylation and ubiquitination in regulation of genome stability. For this post expertise in cell biology is required. my.corehr.com/pls/uoxrecru...

A postdoc position is available in our lab to study ADP-ribosylation and ubiquitylation signalling! We are looking for someone with experience in structural biology and biochemistry. The application deadline is June 20th. my.corehr.com/pls/uoxrecru...

New work @harvard by Miguel Gonzalez-Lozano @harperlabhms.bsky.social & @ernstschmid.bsky.social in Johannes Walter lab charts structural interactome of endosomes. #XL-MS #Alphafold Funded by @asapresearch.parkinsonsroadmap.org & NIH. Science continues-despite attacks www.nature.com/articles/s41...

Protein degradation in E. coli via N-terminal degradation signals (degrons) defined by the @kunjapur.bsky.social lab. @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...

I’m very excited to share our latest publication characterising the substrate binding interactions of an N-terminal cysteine oxidase (NCO), out now in @natcomms.nature.com! doi.org/10.1038/s414...

Sharing the next installment of our fun collaboration with @michaelnadbio.bsky.social at the interface of #ubiquitin and #ADP-ribosylation! Building upon our identification of cellular MARUbylation, we now identify reader/writer E3 ligases that extend K11 polyUb! 🧵 www.biorxiv.org/content/10.1...

Pass it on. #StandWithUkraine

Did you recently complete a ChemBio/MedChem PhD (non-UK) and are looking for the next challenge? I am seeking a prospective applicant for a Newton Fellowship with an exciting project in targeted protein dephosphorylation! Please reach out to [email protected] royalsociety.org/grants/newto...

Thanks to the more than *1100* members of the scientific community who have signed the letter to insist that the Royal Society remains true to values that we all hold dear but that Musk FRS has ridden roughshod over. Please keep signing & sharing. docs.google.com/forms/d/e/1F...

Happy to share our new study showing that the Ub E3 ligase SIAH1, known to dimerise via its C-term SBD, also dimerises via its N-term RING. When these tendencies combine, full-length SIAH1 forms multimers, which might explain its clustering in cells & preference for aggregated/multimeric substrates.

🚨 The #Ubiquitin Congress is back in Würzburg! 🚨 Excited to host it again, June 2-4, 2025! 🌟 ✨ Still time to submit your abstract for a Short Talk! 🗓️ Registration Deadline: March 1st 📝 Abstract Submission Deadline: April 15th Don’t miss out—register now! ➡️ www.ubiquitin-wuerzburg-2025.de

Novel proteins regulated by hypoxia and the Arg/N-degron pathway. Cysteine-starting proteins such as IP6K1 and PIFO are oxidized by ADO, arginylated by ATE1 and ubiquitylated by UBRs. @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...

I am experiencing an alarming increase in papers to review with an inimmaginabile amount of data already at submission. Sometimes 8 figures each with panels from a to z. Ungraspable complexity of an apparent 'mechanism', but often no clear message. Not nice. Has anyone observed this or is it me?

The ASBMB "O-GlcNAc 🟦 in Health and Disease" conference is coming to Duke University, July 11-13, 2025! Thrilled to co-chair with Mike Boyce 🙌 Confirmed speakers in comments👇 Can’t wait to see you there! 🙌 asbmb.org/meetings-eve... #glycotime #ASBMB

Thrilled to share our new paper @Chouchani Lab in Cell; what a great way to end this year! We establish a deep mapping of the zinc-binding human cysteine proteome (ZnCPT) and discover glutathione reductase as zinc-targetable cancer vulnerability. www.cell.com/cell/fulltex...

The mitochondrial NAD+ pool buffers the overall decline in cellular NAD+. Fascinating! Subcellular NAD+ pools are interconnected and buffered by mitochondrial NAD+ www.nature.com/articles/s42...

Hi Bluesky community! 👋 Are you looking forward to 2025 as much as we are? 👀 We just updated our 2025 Annual Poster to include even more events for you to join, so go and have a look! ➡️ s.embl.org/poster-bl #EMBLEvents #molecularbiology #lifesciencetraining

The expanding landscape of canonical and non-canonical protein phosphorylation. New review out in TiBS @cellpress.bsky.social www.cell.com/trends/bioch...

Now that I've fled Leon's site for bluer skies, I'm sure I'll be called an #Ex-X, but personally, I'll always consider myself a #TwitterQuitter.

We strongly suggest that academic publishers and other platforms that host research rapidly implement a Share to Bluesky button for their articles. Here's how: docs.bsky.app/docs/advance... #AcademicSky #HigherEd #Altmetrics