@ahellab.bsky.social
📤 127
📥 50
📝 28
pinned post!
We identify novel catalytically impaired PARP1 mutants and show that the toxicity of some variants in cells can be rescued by the PARP inhibitor saruparib. Thus, in specific contexts, saruparib plays a pro-survival role rather than a cytotoxic one.
www.cell.com/cell-reports...
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PARP1-specific inhibitor displays PARP1-detrapping activity
SchĂĽtzenhofer et al. show that expression of catalytically impaired PARP1 mutants is toxic to cells, which can be overcome by treatment with saruparib, a PARP1-specific inhibitor that displays a pro-r...
https://www.cell.com/cell-reports/fulltext/S2211-1247(26)00428-6
about 1 month ago
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8
2
reposted by
Kurianlab
22 days ago
www.nature.com/articles/s41...
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Widespread DNA off-targeting confounds RNA chromatin occupancy studies - Nature Biotechnology
Many long noncoding RNA–DNA binding peaks detected using common assays arise from technical artifacts.
https://www.nature.com/articles/s41587-026-03130-3
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reposted by
Jarvis Lab
29 days ago
Our new study characterises the assembly of two functionally distinct chloroplast protein import complexes (TOC-P and TOC-N) in the outer membrane of plant chloroplasts. Huge thanks to @Ivan Ahel's lab and
@sykhalid.bsky.social
's lab, for a fantastic collaboration!
www.biorxiv.org/content/10.1...
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https://www.biorxiv.org/content/10.1101/2025.10.27.683105v1
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5
5
reposted by
Alexis Verger 🧬🧫🧪
about 1 month ago
#RIP
Pierre Chambon (1931-2026) via
@alainberetz.bsky.social
one of the last giants in biology: PARP, nuclear receptors, RNA Pol II, nucleosome, promoters, split genes
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We identify novel catalytically impaired PARP1 mutants and show that the toxicity of some variants in cells can be rescued by the PARP inhibitor saruparib. Thus, in specific contexts, saruparib plays a pro-survival role rather than a cytotoxic one.
www.cell.com/cell-reports...
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PARP1-specific inhibitor displays PARP1-detrapping activity
SchĂĽtzenhofer et al. show that expression of catalytically impaired PARP1 mutants is toxic to cells, which can be overcome by treatment with saruparib, a PARP1-specific inhibitor that displays a pro-r...
https://www.cell.com/cell-reports/fulltext/S2211-1247(26)00428-6
about 1 month ago
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Interesting paper on PARP4 and the phosphorylation, sumoylation, and ADP-ribosylation cascade in apoptosis regulation, with a small contribution from our team.
www.science.org/doi/10.1126/...
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PARP4 ADP-ribosylates PIDD1 to complete a phospho/SUMO/PAR-ylation cascade that orchestrates PIDDosome assembly
The orphan PARP, PARP4, is shown to play a critical role in the apoptotic response to unresolved DNA interstrand cross-links.
https://www.science.org/doi/10.1126/sciadv.adz9284
about 1 month ago
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3
1
reposted by
Ryo Yokoyama
about 2 months ago
Assembly of two functionally-distinct protein import complexes in the outer membrane of plant chloroplasts
www.nature.com/articles/s41...
@natcomms.nature.com
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Assembly of two functionally-distinct protein import complexes in the outer membrane of plant chloroplasts - Nature Communications
Chloroplasts import thousands of nucleus-encoded proteins via multiprotein translocons. The authors elucidate the assembly and molecular architecture of the outer-membrane TOC translocon (the import “...
https://www.nature.com/articles/s41467-026-71676-6
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reposted by
Biochemical Society
about 2 months ago
A leader in the cell biology of signalling, the 2027 Centenary Award is presented to
Matthew Freeman
in recognition of his pioneering discoveries into the biological significance and mechanisms of rhomboid intramembrane proteases and pseudoprotease and unwavering commitment to mentorship!
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We show that the E3 ubiquitin ligase DTX2 creates a dual hybrid ADP-ribosyl-ubiquitin mark (ADPr-Ub) at DNA damage sites, driving the recruitment of RNF114, RNF138, and RNF166 proteins independently of HPF1.
journals.plos.org/plosbiology/...
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Specificity and recognition of the ADP-ribosyl-ubiquitin modification in the DNA damage response
Recent studies have shown that ADP-ribose modifications can be further modified by ubiquitin (ADPr-Ub), but there are still major gaps in our knowledge around this modification and its role in the DNA damage response. This study biochemically characterises the amino acid specificity for ADPr-Ub production and its recognition by E3 ubiquitin ligases.
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3003747
about 2 months ago
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Hyperosmotic stress induces PARP1-mediated HPF1-dependent mono(ADP-ribosyl)ation:
febs.onlinelibrary.wiley.com/doi/10.1002/...
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FEBS Press
Sorbitol-induced hyperosmotic stress rapidly induces reversible mono(ADP-ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 depend...
https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.70334
2 months ago
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In this paper, we present the first evidence suggesting that reversible RNA ADP-ribosylation on bases occurs in higher organisms (through the combined action of PARP10 and TARG1):
academic.oup.com/nar/article/...
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Reversible RNA ADP-ribosylation on uracil bases
Abstract. ADP-ribosylation is a conserved modification that uses NAD+ as a co-substrate to regulate essential cellular processes, such as genome stability
https://academic.oup.com/nar/article/54/6/gkag289/8566094
2 months ago
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Check out our new review on PARP14. We cover everything to know about this fascinating enzyme—its structure and evolution, antiviral activities against coronavirus infection, roles in genome stability and inflammation, and its links to ubiquitination signaling.
genesdev.cshlp.org/content/earl...
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Structure, function, regulation, evolution, and therapeutic implications of PARP14
A biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields
https://genesdev.cshlp.org/content/early/2026/03/05/gad.353495.125
3 months ago
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reposted by
Royal Society Publishing
3 months ago
New research from
#OpenBiology
: Histidine and tyrosine residues are targets for SIRT6 ADP-ribosylation activity
royalsocietypublishing.org/rsob/article...
|
#Biochemistry
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In this paper, we demonstrate that the sirtuin SIRT6 can efficiently ADP-ribosylate histidine and tyrosine residues in proteins.
royalsocietypublishing.org/rsob/article...
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Histidine and tyrosine residues are targets for SIRT6 ADP-ribosylation activity
Abstract. SIRT6, a highly conserved member of the sirtuin family, plays a critical role in diverse cellular processes, including gene regulation, DNA damag
https://royalsocietypublishing.org/rsob/article/16/3/250181/480631/Histidine-and-tyrosine-residues-are-targets-for
3 months ago
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Recent discovery have revealed reversible ADP-ribosylation of cytosine bases in nucleic acids:
www.mdpi.com/2072-6651/18...
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https://www.mdpi.com/2072-6651/18/2/82
3 months ago
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1
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reposted by
Luca Palazzo lab
4 months ago
Excited to share our lab’s new article in Biochemical Pharmacology on PARG as a key regulator opposing PARP functions and its emerging therapeutic potential in cancer. Overview of current PARG inhibitors included.
www.sciencedirect.com/science/arti...
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The PARG frontier: mechanisms of PAR turnover and opportunities in precision oncology
ADP-ribosylation is a versatile post-translational modification that governs fundamental processes, including DNA repair, transcription, and stress ad…
https://www.sciencedirect.com/science/article/pii/S0006295226001012
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reposted by
Mike Cohen
5 months ago
Did you miss the original abstract deadline? No worries, we’ve extended it to January 30th! Don’t miss your chance to be part of an exciting meeting highlighting the cutting edge of the field.
add a skeleton here at some point
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The first chemical synthesis of ADP-ribosylated oligonucleotides provides powerful tools to study ADP-ribosylation of nucleic acids:
pubs.acs.org/doi/10.1021/...
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https://pubs.acs.org/doi/10.1021/jacs.5c17532
5 months ago
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We contributed to this newly published article describing the chemical synthesis of ADP-ribosylated histidine, which serves as a signalling molecule in bacterial antiphage systems:
pubs.acs.org/doi/10.1021/...
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Synthesis of Adenosine Diphosphate Ribose Histidine
Adenosine diphosphate ribosylated histidine (His-ADPr) is a vital signaling molecule for the bacterial immune system, the antiphage system in particular. In this unique second messenger molecule, the distal nitrogen of the histidine side chain is ribosylated in a β fashion. Here, we describe the synthesis of this bacterial metabolite and its triazole isostere and evaluate the stability of His-ADPr to relevant ribosyl hydrolases.
https://pubs.acs.org/doi/10.1021/acs.orglett.5c04626
5 months ago
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Learn more about the interplay between ADP-ribosylation and ubiquitination: PARG regulates proteasomal degradation of the ADP-ribosyl hydrolase TARG1 by preventing PAR-dependent ubiquitination mediated by the E3 ligases HUWE1 and TRIP12
www.sciencedirect.com/science/arti...
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PARG regulates the proteasomal degradation of TARG1
ADP-ribosylation (ADPr) is a reversible modification of macromolecules critical for the regulation of genome stability, stress responses, and proteost…
https://www.sciencedirect.com/science/article/pii/S221112472501561X
5 months ago
0
5
3
reposted by
The EMBO Journal
6 months ago
Mono-ADP-ribosylation as a degradation mark?
@andriigorelik.bsky.social
, Ivan Ahel et al observe increased ADP-ribosylation of aryl hydrocarbon receptor (AHR) & PARP7 upon ubiquitin-proteasome blockage, crosstalk with DTX2 E3, as rapid AHR-transcription off-switch
www.embopress.org/doi/full/10....
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The interplay between ADP-ribosylation and ubiquitination, mediated by PARP7 and DTX2 proteins, can serve as a rapid AHR transcription “off-switch” through proteasomal degradation.
www.embopress.org/doi/full/10....
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Ubiquitin pathway blockade reveals endogenous ADP-ribosylation marking PARP7 and AHR for degradation | The EMBO Journal
imageimageDegradation mechanisms of transcriptionally active aryl hydrocarbon receptor (AHR) are unclear. This work reveals that PARP7 ADP-ribosylates itself and ligand-bound AHR, creating a recogniti...
https://www.embopress.org/doi/full/10.1038/s44318-025-00656-1
6 months ago
0
8
0
reposted by
Aude Bernheim
7 months ago
Akin to bacterial SIR2 antiphage proteins, human SIRal, also known as FAM118b, forms filaments that are essential for its NAD processing activity. Awesome to see structures of these filaments that differ from bacterial ones. Congrats to the authors on this beautiful study.
add a skeleton here at some point
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reposted by
Marcin J. Suskiewicz
7 months ago
Very happy to share our collaborative project on FAM118 proteins - noncanonical sirtuins that form filaments and process NAD in human and other vertebrate cells.
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Filament formation and NAD processing by noncanonical human FAM118 sirtuins
Nature Structural & Molecular Biology - Baretić and Missoury et al. identify vertebrate proteins FAM118B and FAM118A as sirtuins similar to bacterial antiphage enzymes and show that...
https://rdcu.be/eQjNK
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Please check our collaborative paper with Marcin Suskiewicz and Sebastien Huet labs on FAM118 proteins – non-canonical sirtuins that form filaments and act as NADases in human and other vertebrate cells.
www.nature.com/articles/s41...
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Filament formation and NAD processing by noncanonical human FAM118 sirtuins - Nature Structural & Molecular Biology
Baretić and Missoury et al. identify vertebrate proteins FAM118B and FAM118A as sirtuins similar to bacterial antiphage enzymes and show that FAM118A/B processing of NAD involves head-to-tail filament...
https://www.nature.com/articles/s41594-025-01715-1
7 months ago
0
16
3
reposted by
Karolin Luger
7 months ago
Beautiful review from J. Rudolph on Histone PARylation factor 1: a review of its role in the DNA damage response url:
academic.oup.com/nar/article/...
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Histone PARylation factor 1: a review of its role in the DNA damage response
Abstract. Although poly-(ADP ribose) polymerase 1 (PARP1) and PARylation of histones have been known for over 50 years and have been successfully targeted
https://academic.oup.com/nar/article/53/20/gkaf1170/8317315
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20
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reposted by
The Dunn School, Oxford
8 months ago
A new study by
@thurstonlab.bsky.social
reveals how a small virulence protein from Salmonella uses molecular mimicry to reprogramme host kinase specificity. Find out more:
www.path.ox.ac.uk/news-article...
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Converting a kinase through molecular mimicry - Dunn School
Published in EMBO Reports, a new study by the Thurston Group reveals how a small virulence protein from Salmonella called SteE, uses molecular mimicry to reprogramme host kinase specificity.
https://www.path.ox.ac.uk/news-article/converting-a-kinase-through-molecular-mimicry/
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Here you can read our full review paper on the interplay between ADP-ribosylation and ubiquitination, with special focus on dual, hybrid modification ADPr-Ub:
pubmed.ncbi.nlm.nih.gov/41065402/
8 months ago
0
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This News & Views article provides a nice overview of the recent discoveries that RNF114 recognizes a dual modification ADP-ribose–ubiquitin (ADPr-Ub) and subsequently extends it by adding additional ubiquitin units.:
pubmed.ncbi.nlm.nih.gov/41039156/
8 months ago
0
2
0
reposted by
The EMBO Journal
8 months ago
RNF114 and RNF166 exemplify a new E3 ligases family that recognizes
#MARUbylation
–hybrid ADP-ribose/ubiquitin modifications– and extends them with K11-linked polyubiquitin
@jnpruneda.bsky.social
@michaelnadbio.bsky.social
and colleagues
www.embopress.org/doi/full/10....
1
8
5
reposted by
9 months ago
The September
@natsmb.nature.com
issue is out!
www.nature.com/articles/s41...
We feature very interesting papers on ubiquitylation and degradation. Really excited to continue to serve the proteostasis and PTMs communities and publish conceptually insightful work.
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Focus on ubiquitylation and protein degradation - Nature Structural & Molecular Biology
We highlight primary research and commissioned content that delve into the biology of ubiquitylation and degradation mechanisms.
https://www.nature.com/articles/s41594-025-01672-9
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9
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reposted by
Nature Biotechnology
9 months ago
Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes -
@helmholtz-hiri.bsky.social
go.nature.com/4n7zDpG
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Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - Nature Biotechnology
Append editing of ADP-ribosyl to thymine is used for precise modifications in bacteria and eukaryotes.
https://go.nature.com/4n7zDpG
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reposted by
The Dunn School, Oxford
9 months ago
Our PhD studentship programme is officially open; pass it on If you are looking for a fully-funded PhD in the mechanisms underlying human disease, here are the details... Great science and a great environment!
www.path.ox.ac.uk/news-article...
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Applications now open for our PhD studentship programme - Dunn School
Applications are now open to apply for our four year, fully-funded PhD programme in Molecular Cell Biology in Health and Disease.
https://www.path.ox.ac.uk/news-article/applications-now-open-for-our-phd-programme-2/
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A few years back we discovered a dual hybrid protein modification composed of an ADP-ribose dinucleotide and the ubiquitin moieties (ADPr-Ub). Here you can read our review that will give you an update on this increasingly popular topic:
rdcu.be/eETIT
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The rise of ADP-ribose–ubiquitin
Nature Structural & Molecular Biology - Post-translational modifications show mechanistic crosstalk, exemplified by the ADP-ribose–ubiquitin hybrid signal, in which one post-translational...
https://rdcu.be/eETIT
9 months ago
0
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We have postdoc positions available to study roles of ADP-ribosylation and ubiquitination signalling in genome stability, infection or immunity. Please enquire if you are interested.
9 months ago
0
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2
reposted by
The Dunn School, Oxford
10 months ago
📣 Applications now open 📣 We are seeking outstanding ECRs looking for a stimulating and supportive environment in which to establish their research group as externally-funded fellows, in any area or cell or molecular biology underlying disease! Please share!
www.path.ox.ac.uk/work-with-us...
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reposted by
Alessandro Costa
11 months ago
Less than two weeks left to apply! Postdoc positions in my lab to study 1. initiation of DNA replication. 2. chromatin replication/epigenetic inheritance. Great for biochemists, biophysicists & structural biologists. Deadline 3 August 2025.
crick.wd3.myworkdayjobs.com/External/job...
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Postdoctoral Fellow - Costa Lab
Salary for this Role: From ÂŁ45,500 with benefits, subject to skills and experience. Job Title: Postdoctoral Fellow - Costa Lab Reports to: Alessandro Costa Closing Date: 03/Aug/2025 23.59 GMT Job Desc...
https://crick.wd3.myworkdayjobs.com/External/job/London/Postdoctoral-Fellow---Costa-Lab_R2283-2
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The application deadline has been extended for a postdoc position in our lab until August 22. Please enquire/apply if you are interested in ADP-ribosylation, ubiquitination, genome stability or immunity.
my.corehr.com/pls/uoxrecru...
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https://my.corehr.com/pls/uoxrecruit/erq_jobspec_details_form.jobspec?p_id=181069
11 months ago
0
2
8
reposted by
Rob Klose
11 months ago
My wonderful colleage Colin Kleanthous is raising money for the MND association. Please support Colin in this worthy endeavor!
www.justgiving.com/page/colin-k...
(Please repost!)
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Colin Kleanthous' fundraiser for Motor Neurone Disease Association
Help Colin Kleanthous raise money to support Motor Neurone Disease Association
https://www.justgiving.com/page/colin-kleanthous-iveagh
0
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Defence island in Escherichia coli composed of DarTG toxin-antitoxin system embedded within a Type I restriction-modification system is regulated by the RptR (RMS-proximal transcriptional regulator).
academic.oup.com/nar/article/...
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Regulation of a phage defence island by RptR, a novel repressor that controls restriction–modification systems in diverse bacteria
Abstract. Bacteria encode a panoply of defence systems to overcome phage infection. In recent years, over 100 defence systems have been identified, with th
https://academic.oup.com/nar/article/53/13/gkaf645/8198042
11 months ago
0
5
1
A few years back we discovered a dual hybrid protein modification composed of ADP-ribose dinucleotide and ubiquitin (ADPr-Ub). Now we reveal that ADPr-Ub can be further ubiquitinated by the E3 ubiquitin ligase RNF114!
www.nature.com/articles/s41...
www.science.org/doi/10.1126/...
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Identification of RNF114 as ADPr-Ub reader through non-hydrolysable ubiquitinated ADP-ribose - Nature Communications
Deltex E3s modify ADP-ribosylated targets with ubiquitin, creating a hybrid modification whose readers remains unknown. Here, the authors synthesise a non-hydrolysable probe that mimics the modificati...
https://www.nature.com/articles/s41467-025-61111-7
11 months ago
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Our review article 'PARPs and ADP-ribosyl hydrolases in cancer therapy: from drug targets to biomarkers' is now available online.
doi.org/10.1016/j.dn...
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Redirecting
https://doi.org/10.1016/j.dnarep.2025.103863
11 months ago
0
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We have a postdoc position available to work on interplay between ADP-ribosylation and ubiquitination in regulation of genome stability and/or immunity. Expertise in cell biology is required.
my.corehr.com/pls/uoxrecru...
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https://my.corehr.com/pls/uoxrecruit/erq_jobspec_details_form.jobspec?p_id=180153
11 months ago
0
2
3
reposted by
Andrii Gorelik
12 months ago
New paper! We uncover mechanistic insights into the crosstalk between AHR (aryl hydrocarbon receptor, a transcription factor) and PARP7 (a nuclear enzyme that performs protein ADP-ribosylation). Both are promising cancer drug targets. Big thanks to all co-authors!
www.pnas.org/doi/10.1073/...
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PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
https://www.pnas.org/doi/10.1073/pnas.2424985122
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Our new paper is out! Trying to understand the molecular function of the PARP7, a PARP family enzyme with links to innate immunity and environmental toxins response. Congrats to Andrii and the rest of the team and collaborators.
www.pnas.org/doi/10.1073/...
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CRISPR screens and quantitative proteomics reveal remodeling of the aryl hydrocarbon receptor–driven proteome through PARP7 activity | PNAS
PARP7 is an enzyme that uses donor substrate NAD+ to attach a single ADP-ribose moiety onto proteins related to immunity, transcription, and cell g...
https://www.pnas.org/doi/10.1073/pnas.2424985122
12 months ago
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We have another postdoc position available to work on interplay between ADP-ribosylation and ubiquitination in regulation of genome stability. For this post expertise in cell biology is required.
my.corehr.com/pls/uoxrecru...
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https://my.corehr.com/pls/uoxrecruit/erq_jobspec_details_form.jobspec?p_id=180153
about 1 year ago
0
1
1
reposted by
Kat Toropova
about 1 year ago
⏰ Deadline approaching for postdoc position in my lab, Dunn School, University of Oxford. We work on how the cell's antenna receives and transmits essential signals through motor protein driven transport. ❄️ Cryo-EM/ET, smTIRF, cell biology methods 🧪🔬 30/5/25 ➡️
tinyurl.com/KToropovaPDRA
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reposted by
SP@RTE
about 1 year ago
Two year postdoc position available in the Sparte lab at IGDR to investigate the crosstalk between ADP-ribosylation and SUMOylation signaling at stressed replication forks
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5
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reposted by
Juraj Ahel
about 1 year ago
Happy that my work on RNF213 has been published as a part of
doi.org/10.1038/s414...
! Many thanks to all the groups involved:
@clausenlab.bsky.social
@e3chembio.bsky.social
@fletcherlab.bsky.social
Have a look at the summary from Fletcher lab for an insider perspective of the paper's history:
add a skeleton here at some point
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A few years back we discovered a novel dual hybrid protein modification composed of ADP-ribose dinucleotide and ubiquitin (ADPr-Ub):
pubmed.ncbi.nlm.nih.gov/36197986/
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| bioRxiv
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
https://www.biorxiv.org/content/10.1101/2025.05.08.652854v1modification
about 1 year ago
1
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A postdoc position is available in our lab to study ADP-ribosylation and ubiquitylation signalling! We are looking for someone with experience in structural biology and biochemistry. The application deadline is June 20th.
my.corehr.com/pls/uoxrecru...
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https://my.corehr.com/pls/uoxrecruit/erq_jobspec_details_form.jobspec?p_id=179710
about 1 year ago
1
19
25
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