Our new study characterises the assembly of two functionally distinct chloroplast protein import complexes (TOC-P and TOC-N) in the outer membrane of plant chloroplasts. Huge thanks to @Ivan Ahel's lab and @sykhalid.bsky.social's lab, for a fantastic collaboration! www.biorxiv.org/content/10.1...

#RIP Pierre Chambon (1931-2026) via @alainberetz.bsky.social one of the last giants in biology: PARP, nuclear receptors, RNA Pol II, nucleosome, promoters, split genes

We identify novel catalytically impaired PARP1 mutants and show that the toxicity of some variants in cells can be rescued by the PARP inhibitor saruparib. Thus, in specific contexts, saruparib plays a pro-survival role rather than a cytotoxic one. www.cell.com/cell-reports...

Interesting paper on PARP4 and the phosphorylation, sumoylation, and ADP-ribosylation cascade in apoptosis regulation, with a small contribution from our team. www.science.org/doi/10.1126/...

Assembly of two functionally-distinct protein import complexes in the outer membrane of plant chloroplasts www.nature.com/articles/s41... @natcomms.nature.com

A leader in the cell biology of signalling, the 2027 Centenary Award is presented to Matthew Freeman in recognition of his pioneering discoveries into the biological significance and mechanisms of rhomboid intramembrane proteases and pseudoprotease and unwavering commitment to mentorship!

We show that the E3 ubiquitin ligase DTX2 creates a dual hybrid ADP-ribosyl-ubiquitin mark (ADPr-Ub) at DNA damage sites, driving the recruitment of RNF114, RNF138, and RNF166 proteins independently of HPF1. journals.plos.org/plosbiology/...

Hyperosmotic stress induces PARP1-mediated HPF1-dependent mono(ADP-ribosyl)ation: febs.onlinelibrary.wiley.com/doi/10.1002/...

In this paper, we present the first evidence suggesting that reversible RNA ADP-ribosylation on bases occurs in higher organisms (through the combined action of PARP10 and TARG1): academic.oup.com/nar/article/...

Check out our new review on PARP14. We cover everything to know about this fascinating enzyme—its structure and evolution, antiviral activities against coronavirus infection, roles in genome stability and inflammation, and its links to ubiquitination signaling. genesdev.cshlp.org/content/earl...

New research from #OpenBiology: Histidine and tyrosine residues are targets for SIRT6 ADP-ribosylation activity royalsocietypublishing.org/rsob/article... | #Biochemistry

In this paper, we demonstrate that the sirtuin SIRT6 can efficiently ADP-ribosylate histidine and tyrosine residues in proteins. royalsocietypublishing.org/rsob/article...

Recent discovery have revealed reversible ADP-ribosylation of cytosine bases in nucleic acids: www.mdpi.com/2072-6651/18...

Excited to share our lab’s new article in Biochemical Pharmacology on PARG as a key regulator opposing PARP functions and its emerging therapeutic potential in cancer. Overview of current PARG inhibitors included. www.sciencedirect.com/science/arti...

Did you miss the original abstract deadline? No worries, we’ve extended it to January 30th! Don’t miss your chance to be part of an exciting meeting highlighting the cutting edge of the field.

The first chemical synthesis of ADP-ribosylated oligonucleotides provides powerful tools to study ADP-ribosylation of nucleic acids: pubs.acs.org/doi/10.1021/...

We contributed to this newly published article describing the chemical synthesis of ADP-ribosylated histidine, which serves as a signalling molecule in bacterial antiphage systems: pubs.acs.org/doi/10.1021/...

Learn more about the interplay between ADP-ribosylation and ubiquitination: PARG regulates proteasomal degradation of the ADP-ribosyl hydrolase TARG1 by preventing PAR-dependent ubiquitination mediated by the E3 ligases HUWE1 and TRIP12 www.sciencedirect.com/science/arti...

Mono-ADP-ribosylation as a degradation mark? @andriigorelik.bsky.social, Ivan Ahel et al observe increased ADP-ribosylation of aryl hydrocarbon receptor (AHR) & PARP7 upon ubiquitin-proteasome blockage, crosstalk with DTX2 E3, as rapid AHR-transcription off-switch www.embopress.org/doi/full/10....

The interplay between ADP-ribosylation and ubiquitination, mediated by PARP7 and DTX2 proteins, can serve as a rapid AHR transcription “off-switch” through proteasomal degradation. www.embopress.org/doi/full/10....

Akin to bacterial SIR2 antiphage proteins, human SIRal, also known as FAM118b, forms filaments that are essential for its NAD processing activity. Awesome to see structures of these filaments that differ from bacterial ones. Congrats to the authors on this beautiful study.

Very happy to share our collaborative project on FAM118 proteins - noncanonical sirtuins that form filaments and process NAD in human and other vertebrate cells.

Please check our collaborative paper with Marcin Suskiewicz and Sebastien Huet labs on FAM118 proteins – non-canonical sirtuins that form filaments and act as NADases in human and other vertebrate cells. www.nature.com/articles/s41...

Beautiful review from J. Rudolph on Histone PARylation factor 1: a review of its role in the DNA damage response url: academic.oup.com/nar/article/...

A new study by @thurstonlab.bsky.social reveals how a small virulence protein from Salmonella uses molecular mimicry to reprogramme host kinase specificity. Find out more: www.path.ox.ac.uk/news-article...

Here you can read our full review paper on the interplay between ADP-ribosylation and ubiquitination, with special focus on dual, hybrid modification ADPr-Ub: pubmed.ncbi.nlm.nih.gov/41065402/

This News & Views article provides a nice overview of the recent discoveries that RNF114 recognizes a dual modification ADP-ribose–ubiquitin (ADPr-Ub) and subsequently extends it by adding additional ubiquitin units.: pubmed.ncbi.nlm.nih.gov/41039156/

RNF114 and RNF166 exemplify a new E3 ligases family that recognizes #MARUbylation –hybrid ADP-ribose/ubiquitin modifications– and extends them with K11-linked polyubiquitin @jnpruneda.bsky.social @michaelnadbio.bsky.social and colleagues www.embopress.org/doi/full/10....

The September @natsmb.nature.com issue is out! www.nature.com/articles/s41... We feature very interesting papers on ubiquitylation and degradation. Really excited to continue to serve the proteostasis and PTMs communities and publish conceptually insightful work.

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - @helmholtz-hiri.bsky.social go.nature.com/4n7zDpG

Our PhD studentship programme is officially open; pass it on If you are looking for a fully-funded PhD in the mechanisms underlying human disease, here are the details... Great science and a great environment! www.path.ox.ac.uk/news-article...

A few years back we discovered a dual hybrid protein modification composed of an ADP-ribose dinucleotide and the ubiquitin moieties (ADPr-Ub). Here you can read our review that will give you an update on this increasingly popular topic: rdcu.be/eETIT

We have postdoc positions available to study roles of ADP-ribosylation and ubiquitination signalling in genome stability, infection or immunity. Please enquire if you are interested.

📣 Applications now open 📣 We are seeking outstanding ECRs looking for a stimulating and supportive environment in which to establish their research group as externally-funded fellows, in any area or cell or molecular biology underlying disease! Please share! www.path.ox.ac.uk/work-with-us...

Less than two weeks left to apply! Postdoc positions in my lab to study 1. initiation of DNA replication. 2. chromatin replication/epigenetic inheritance. Great for biochemists, biophysicists & structural biologists. Deadline 3 August 2025. crick.wd3.myworkdayjobs.com/External/job...

The application deadline has been extended for a postdoc position in our lab until August 22. Please enquire/apply if you are interested in ADP-ribosylation, ubiquitination, genome stability or immunity. my.corehr.com/pls/uoxrecru...

My wonderful colleage Colin Kleanthous is raising money for the MND association. Please support Colin in this worthy endeavor! www.justgiving.com/page/colin-k... (Please repost!)

Defence island in Escherichia coli composed of DarTG toxin-antitoxin system embedded within a Type I restriction-modification system is regulated by the RptR (RMS-proximal transcriptional regulator). academic.oup.com/nar/article/...

A few years back we discovered a dual hybrid protein modification composed of ADP-ribose dinucleotide and ubiquitin (ADPr-Ub). Now we reveal that ADPr-Ub can be further ubiquitinated by the E3 ubiquitin ligase RNF114! www.nature.com/articles/s41... www.science.org/doi/10.1126/...

Our review article 'PARPs and ADP-ribosyl hydrolases in cancer therapy: from drug targets to biomarkers' is now available online. doi.org/10.1016/j.dn...

We have a postdoc position available to work on interplay between ADP-ribosylation and ubiquitination in regulation of genome stability and/or immunity. Expertise in cell biology is required. my.corehr.com/pls/uoxrecru...

New paper! We uncover mechanistic insights into the crosstalk between AHR (aryl hydrocarbon receptor, a transcription factor) and PARP7 (a nuclear enzyme that performs protein ADP-ribosylation). Both are promising cancer drug targets. Big thanks to all co-authors! www.pnas.org/doi/10.1073/...

Our new paper is out! Trying to understand the molecular function of the PARP7, a PARP family enzyme with links to innate immunity and environmental toxins response. Congrats to Andrii and the rest of the team and collaborators. www.pnas.org/doi/10.1073/...

We have another postdoc position available to work on interplay between ADP-ribosylation and ubiquitination in regulation of genome stability. For this post expertise in cell biology is required. my.corehr.com/pls/uoxrecru...

⏰ Deadline approaching for postdoc position in my lab, Dunn School, University of Oxford. We work on how the cell's antenna receives and transmits essential signals through motor protein driven transport. ❄️ Cryo-EM/ET, smTIRF, cell biology methods 🧪🔬 30/5/25 ➡️ tinyurl.com/KToropovaPDRA

Two year postdoc position available in the Sparte lab at IGDR to investigate the crosstalk between ADP-ribosylation and SUMOylation signaling at stressed replication forks

Happy that my work on RNF213 has been published as a part of doi.org/10.1038/s414...! Many thanks to all the groups involved: @clausenlab.bsky.social @e3chembio.bsky.social @fletcherlab.bsky.social Have a look at the summary from Fletcher lab for an insider perspective of the paper's history:

A few years back we discovered a novel dual hybrid protein modification composed of ADP-ribose dinucleotide and ubiquitin (ADPr-Ub): pubmed.ncbi.nlm.nih.gov/36197986/

A postdoc position is available in our lab to study ADP-ribosylation and ubiquitylation signalling! We are looking for someone with experience in structural biology and biochemistry. The application deadline is June 20th. my.corehr.com/pls/uoxrecru...