Fascinating work from @tianyang22.bsky.social and Yan Cao who discovered that SPIN90 dimerises to nucleate bidirectional filaments! #Arp2/3 #cryoEM

The Dunn School PhD programme is live! 🎉 Two projects in our labs: 1) How the motor protein giant dynein-2 works 2) How signalling-essential membrane proteins get into cilia Cryo-EM/ET, single mol & live-cell fluor microscopy, CRISPR-based genetic tools & more! www.path.ox.ac.uk/study-with-u...

OMG the newest Coot is on Flathub! 🤩 I wish I had known earlier. https://flathub.org/apps/io.github.pemsley.coot #CryoEM

Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social www.biorxiv.org/cgi/content/... @mrclmb.bsky.social @wellcometrust.bsky.social

Relieved that kids go back to school today, the train to work is peaceful again!

Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1... Soon at @spirochrome.com Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social

Was already using 3 degree increments but very useful benchmarking to determine how the chosen tilts affect the resulting data!

Very excited to announce that my first, first author paper, is now on BioRxiv! In this paper we unambiguously show #COPII coated vesicles in unperturbed human cells for the very first time!! Make my day and check it out! www.biorxiv.org/content/10.1... #Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬

Excited to share the latest collaborative work from our lab at @ulethbridge.ca! We show that zyxin directly interacts with nuclear DNA to regulate transcription of mitochondrial pseudogenes—highlighting a novel role for zyxin in mitochondrial function. cdnsciencepub.com/doi/10.1139/...

I made a little doodle this weekend showing how I felt when I saw my first Titan Krios in person. That was quite a few years ago now, amazing how much has changed in the cryoEM field since then.

Vimentin intermediate filaments coordinate actin stress fibers and podosomes to determine the extracellular matrix degradation by macrophages www.cell.com/developmenta...

Are you looking for a postdoc? I will be looking for 2-4 people after the summer to join the lab !! Do not hesitate to reach out and disseminate !!

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

Still thinking about the presentations and discussions had at CCP-EM last week, such a fantastic meeting for the cryoEM community. Managed to get a box of subpar hexAuFoils free, trying to think of something creative to make with dead grids 😅

Postdoc position in my lab: investigating how the microtubule motor protein dynein-2 works in cilia. Here's dynein-2 at work translocating microtubules - how is this activity regulated? How does dynein-2 malfunction in disease? Find out more and apply: my.corehr.com/pls/uoxrecru...

New Title Alert: Tomotools- a tool that provides scripts for making cryo-electron tomography a bit easier. Learn more here: buff.ly/Dz1LT1I #SBGrid #Software #StructuralBiology

The @ccpem.bsky.social Spring Symposium starts today! Watch the live stream at ukri.zoom.us/j/93883619401 Schedule can be found at tinyurl.com/wx238aya Open-access Symposium Proceedings going back to 2016 can be found in @actacrystd.iucr.org at journals.iucr.org/d/services/c...

A colder frontier in cryo-EM 🧪🔬 Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins). Read more: tinyurl.com/mwwcunkc #LMBResearch

Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...

One of the best courses for those wanting to learn about EM! Come join us in London 🚇

This is the meeting I always look forward to the most each year! Well worth attending if you can.

The ever popular EMBO practical course in cryoEM image processing, to be held at Birkbeck College London from 9th to the 16th September, is now open for registrations meetings.embo.org/event/25-cry...

Latest paper from the lab: the Cryo-EM structure of BeeR! (Yes we did name the protein. No it’s not a coincidence)

Sad news about Karen Davies' passing. Karen was an inspiration in bioenergetics and cryoET. Her pioneering postdoctoral work on ATP synthase and respiratory supercomplexes marked turning points in the field. I created Scholar profile to acknowledge her contribution scholar.google.com/citations?hl...

📢 Registration is now open for 'Wellcome-MRC Cryo-EM in Structural Biology 2025'! 📅 Join us online March 3-7 at @diamondlightsource.bsky.social @universityofleeds.bsky.social for lectures & demos in #CryoEM & #CryoET: sample prep, SPA, model building & more!🧬 🔗 www.diamond.ac.uk/Instruments/...

Great start to #bps2025 with a lot of awesome research in the cryoEM Symposium. Looking forward to discovering more!

Hi all- we are searching for a new manager/operator for KCL's Cryo-EM Structural Discovery Facility: a JEOL cryoARM200 + k3 and sample preparation equipment. It's producing excellent high-resolution data using serialEM and Gatan Latitude (EPU-like)! Apply below! www.kcl.ac.uk/jobs/107305-...

Been fostering cats for the last few months and this boy is certainly the most derpy so far! So adorable, just had to share

Been fostering cats for the last few months and this boy is certainly the most derpy so far! So adorable, just had to share

Structural biology inside multicellular specimens using electron cryotomography by @idocaspy.bsky.social @zhexinwang.bsky.social out now in Quarterly Reviews of Biophysics doi.org/10.1017/S003...

Working on actin cytoskeleton and Arp2/3 complex? Check out our latest work～https://www.biorxiv.org/content/10.1101/2025.01.31.635869v1 In this work, we found SPIN90 forms a dimer, facilitating bidirectional actin filament nucleation by activating two Arp2/3 complex #Cryo-EM #Actin #Arp2/3

Arp2/3 nucleates bidirectional linear actin filaments when activated by SPIN90 !!! www.biorxiv.org/content/10.1...

Excited to be attending my first BPS meeting this year! Only a couple weeks left 😬

X/Twitter account deactivated. I'll miss what we had there but glad to be free of it!

*Fully funded PhD position available in the lab* We will use structural proteomics to map the mechanosensitive interactions of talin in dendritic spines and how they change during long term potentiation. Please share, and email for details :) www.findaphd.com/phds/project...

There are now 24 step-by-step guides, explaining how to create these ⬇️ data visualizations with R/ggplot2 - I hope this is a useful resource. Find them all here: joachimgoedhart.github.io/DataViz-prot...

Synchronized filopodial formation for #FluorescenceFriday 💥 #CellBiology #Microscopy @vucellimaging.bsky.social

Our new study shows that SARS-CoV-2 spike protein accumulates & persists in the body for years after infection, especially in the skull-meninges-brain axis, potentially driving long COVID. mRNA vaccines help but cannot stop it🔬🧠🦠🧵Your weekend read👇 @cellpress.bsky.social cell.com/cell-host-mi...

ÅNGSTRÖM-RESOLUTION FLUORESCENCE MICROSCOPY. Can we do structural biology with an optical microscope? Yes! Check out how we resolved *single bases of DNA* and much more using fluorescence microscopy, out on 24.05.2023 in Nature! @natureportfolio.bsky.social [re-post from Twitter]

Goodness that's a lot of follows! Hii 👋🏼

To the #CryoET folks: Is there any advantage to collecting tilt series in EER (vs. good old Tiff format.) for in situ CryoET? I saw a meeting report from July 2024 on the web related to this question, but could not find a publication (yet).

😮 Exciting study from G. Zanetti's lab exploring COPII assembly on reconstituted membrane carriers and microsomes by #CryoET: rdcu.be/dZK8M ❄️🔬

Here is a starter pack for CryoEM/ET enthousiasts To be completed go.bsky.app/JmQksC

For folks just arriving in #AcademicBsky or #ScienceBsky, here are a couple of Biophysics starter packs. Biophysicists, pls tag yourself and others and I will add you to the new starter packs. go.bsky.app/4AUjqVz

It's only called Science Twitter if it comes from arguing on working hours and banana ripeness. Otherwise it's just sparkling procrastination

Awesome to see more scientists venturing over to bluesky! X has become so toxic with all the adverts and politics it wasn't a place I wanted to be anymore 😥

Made a starter pack for microscopy and image analysis - please (self-)nominate additions! go.bsky.app/Nwa91pC