💥 Quick thread on some stunning actin filament swirls🌪️ spotted under platinum replica EM, often forming nest-like structures around caveolae🏐 #ActinAesthetics #ElectronMicroscopy

Happy Friday! Here to bring a bit of brightness to your day are a few images by Julissa Burgos, one of our graduate students in the Tyska lab here at Vanderbilt. Gorgeous!! 🔬 @yuyi106.bsky.social @tyskalabactual.bsky.social #fluorescencefriday #cellbiology

This is couple of years old since I rarely have time to sit at a scope these days but I was inspired by the end of year #FluorescenceFriday posts on hear and thought I should drop on y’all my Eye of Sauron, aka a mouse oocyte containing nuclear actin filaments #actin #meiosis #oocytes

Happy #FluorescenceFriday! These are polarized epithelial cells with microvilli on their apical surface! Captured using the #Nikon NSPARC, and color-coded by depth #actin #microscopy @tyskalabactual.bsky.social

This one is for the holiday season

Thought Spef1 one was some ciliary protein? Check out Dr. Rocio‘s (@rtpastrana.bsky.social) poster on how Spef1 shapes epithelial monolayers, no cilia needed 🤩. Tuesday at #cellbio2024, P2439, B46. @vucellimaging.bsky.social

Have you always wondered how microvilli form? Where DOES that actin come from to form the core bundle? Come to the #BuildingTheCell (Part 1) subgroup tomorrow morning at 8:45 to find out! Room 29C. #CellBio2025 @tyskalabactual.bsky.social

Also, also on Monday at #cellbio2024 I get to present Olivia’s (@operkins.bsky.social) unexpected discoveries on the link between clathrin-mediated endocytosis and the growth of epithelial microvilli 🤯 P1831. B137. @vucellimaging.bsky.social

Also Monday at #cellbio2024! Julissa (@yuyi106.bsky.social) will present her studies on the role of an I-BAR protein (IRTKS) in the pathogenic mechanism of Enterohemorrhagic E. coli 🦠🧫🔬 P2352. B677. @vucellimaging.bsky.social

Posters on tuft cells are about as rare as tuft cells, but at #cellbio2024 on Monday, @jen-silverman.bsky.social presents “P1718. Organization of a cytoskeletal superstructure in the apical domain of intestinal tuft cells. B23” Yes! those are gigantic actin bundles 🤯 @vucellimaging.bsky.social

Synchronized filopodial formation for #FluorescenceFriday 💥 #CellBiology #Microscopy @vucellimaging.bsky.social

Intestinal #organoid goes rogue and flips polarity 🤯 @vucellimaging.bsky.social #epithelial #cellbiology #microscopy

It's that time of year. Confocal + Christmas = Xmas Tree, Ymas Tree, and our favorite Zmas tree. #microscopy #confocal #imaging

As promised, here a fast acquisition of a migrating cell videoed under a microscopy, look those membrane dynamics @ the leading edge ! Bonus: nuclear deformation once the cell rear snaps. (Fluo nucleus, for the #fluorescencefriday) @focalplane.bsky.social @cellcommlab.bsky.social #CellMigration 🧪🔬

Highlighting our recent paper on how we think doublecortin (green) contributes to growth cone biomechanics in developing neurons for #FluorescenceFriday www.cell.com/current-biol...

When you’re imaging and your sample takes a Pikachu! Having fun playing around with our new #Nikon NSPARC for #FluorescenceFriday #microscopy #myosin #epithelial #cellbiology #meme @tyskalabactual.bsky.social

O is for #organoid @vucellimaging.bsky.social #microscopy #epithelial #cellbiology

The real IFT… intra💥filopodial💥transport. @vucellimaging.bsky.social #cytoskeleton #biology #microscopy

Starting the week strong! Cheering myself up for the next challenges with 15 min of axonal growth cone dynamic madness. F-actin (cyan) and membrane (magenta) show their dance. One image taken every 10 s.🧠🔬 #MicroscopyMonday #LiveImaging #Neuroscience #Microscopy

Good sampling on this confocal volume of small intestinal tissue showing the apical shag carpet (blue is F-actin, magenta is ZO1)🔬🤩 @vucellimaging.bsky.social #microscopy #cytoskeleton #epithelial #cellbiology #biology

Super proud of pre-Dr. Julissa for going from ND to fundable score with a single revision of her F31 NRSA app🤩🙌🍾🥂🎉🦠🔬 @yuyi106.bsky.social

Extremely rare microtubule staining happening in the lab right now 😭

First #FluorescenceFriday here on 🦋 Hope you enjoy this cross-section of the intestinal epithelium, highlighting the actin within the #brushborder @tyskalabactual.bsky.social

Admittedly, this is a reversed movie of #microvilli collapsing, but how cool would it be if growth were synchronized like this? #FluorescenceFriday @tyskalabactual.bsky.social

A cell undergoing cell division videoed through a microscope. Chromosomes are shown in pink. Differential Interference Contrast (DIC) Microscopy is shown in cyan. #Science #Biology #CellBiology #microscopy #medsky

Hello Bluesky! First post is a favorite image of the collagen and vascular network in the dermis of a living 🐁 🔬: 2-Photon

As my official introduction to the blue sky. Here is a TIRF image of #HeLa cells forming and elongating its beautiful #filopodia! @tyskalabactual.bsky.social

I'm excited to rebuild this #science community over here on Bluesky! In honor of this clean slate, here's a repost of my favorite #epithelial cell building its brush border. @tyskalabactual.bsky.social

Slicing through an intestinal organoid on the spinning disk, these cells are really dynamic 🏎️ before tissue polarity sets in. @vucellimaging.bsky.social

Inaugural post 🦋 The apical microvilli of intestinal epithelial cells create dazzling displays as they assemble the #brushborder! This image was acquired on the remarkable #Nikon NSPARC in @tyskalabactual.bsky.social

We don’t study dividing cells, but I can respect what they’re trying to do. And always nice watching stress fibers blow up 💥🙏 @vucellimaging.bsky.social

This is a movie of actin filaments in a cultured cell. I like watching it and I hope you do to.

mStayGold on endogenous EPS8 🔥🔥🔥 courtesy of pre-Dr. Kianna Robinson 🙏🙌 Dear EGFP, thanks for all the data, it’s been nice knowing you. @vucellimaging.bsky.social