Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup). A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.

A molecular-resolution look into the near-native architecture of the spinach chloroplast🌱. This one was a long time in the oven, but we're happy to finally share our "version of record". What long-standing debates did we settle? Check back for a short thread🧵 on Monday. #TeamTomo #PlantScience 🧪🧶🧬🔬🌾

Absolutely!

If you are thinking about the future of structural biology, we are too! Our two cents (Jürgen Plitzko and I) just got published online here: authors.elsevier.com/sd/article/S...

New Title Alert: AITom- an open-source platform for AI driven cellular electron cryo-tomography analysis. Learn more here: buff.ly/LLGhjvY #SBGrid #SBGridSoftware #StructuralBiology

New preprint: In situ Architecture of #Plasmodesmata. Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores. 🔗 doi.org/10.1101/2025... 🧪🧵1/n #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM

Our methods paper made it to bioRxiv: doi.org/10.1101/2025... Full #cryoET workflows for #plant tissues. Plus some general tricks and tweaks on serial Lift-Out and cryo-FLM. 🧪🧵1/5 #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM #CLEM

'cux good data don't wait!

Outstanding work from Christine, Stan and the whole team on this beautiful story. Wow.

Neural net picker with a truly general model - no per dataset training required (at least if it works as advertised). Want to try this on some messy/mixed samples where other approaches struggle!

Running Phenix and stuck? Now you can ask the new Phenix chatbot anything from the 600-page tutorial about how to run any program. Big thanks to Tom Willinger for making this happen! Try it out: phenix-online.org/version_docs...

Frostbyte 🥶 socks provided by @hammerspace.com

And it’s done! Thanks to everyone who came out for the first edition of FrostByte, our event for the local computational cryo-EMers in the Bay Area ❄️ Special thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing 🙏

This paper combines two sort-of-crazy (in the best sense) approaches in cryoEM sample preparation: (1) Native, soft-landing electrospray ion beam deposition, followed by (2) deposition of amorphous ice, and reheating and freezing by laser flash melting. Has potential for time-resolved studies.

Dual-axis cryo-ET on cryo-FIB milled lamella 🙂 www.researchsquare.com/article/rs-6... #CryoEM

This is a view from the surface of Comet 67P. The busy particles in the foreground are cosmic rays or bits of dust and ice. The dots moving in the background are stars.

It's hot in there

Excited to share our new @science.org paper! Led by postdocs Ruchao Peng and Xin Xu, we used cryo-EM/ET to reveal the influenza ribonucleoprotein complex structure and its strand-sliding mechanism for RNA synthesis, paving the way for new antivirals. www.science.org/doi/10.1126/...

Mass Spec + lasers + cryoEM = COOL SCIENCE! New research from @coonlab.bsky.social and the Grant Lab combines mass spec to land proteins on TEM grids, a laser to restore protein structure, and cryoEM imaging and analysis to produce 3D reconstructions. www.mcponline.org/article/S153...

In the Bay Area? Love computational cryo-EM/ET? Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community! Details and registration at frostbyte-cryoem.github.io Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

"Science is an investment. We will put forward a new 500 million package for 2025-2027 to support the best and the brightest researchers and scientists from Europe and around the world." — President @vonderleyen.ec.europa.eu at the ‘Choose Europe for Science' event at La Sorbonne 🇫🇷

Sad

Jeff Bezos is a decidedly unfit owner for the Washington Post if this is all it takes to get him to fold. Profile in non courage. www.cnbc.com/2025/04/29/w...

Everyone please read and act! 👇

We have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples! Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵 #CryoET #TeamTomo www.biorxiv.org/content/10.1...

We are trying to contact the person in charge of processing payments for the purchase of a SHELX licence. We get no response and this is a major road block. Anyone has a responsive contact within Goettingen university that could help us? Thanks,

We just need to turn the negative productivity of scientists at NASA and IRS auditors that DOGE fired to gainfully making shoes in Nike factories reshored from Vietnam. This is the groundbreaking economic thinking of communist purges from Mao to Pol Pot with a sprinkle of American Exceptionalism.

Remember “climategate” with the email leaks? Here’s the best explanation of it, in some detail, I’ve ever come across. Great intro to climate science, and to science in general. podcasts.apple.com/us/podcast/c...

The third and final chapter of our webinar series “Cryo-EM: Back to Basics” is on demand. Hear from Giovanna about challenges in #cryoEM, how to overcome them. Catch all three chapters: Chapter 1: youtu.be/e8GyDe4_BAQ Chapter 2: youtu.be/2dBnPVkaoFs Chapter 3: youtu.be/kUGNSTGc8II

Participants in the annual serial crystallography workshop for graduates student some funded by our @wellcometrust.bsky.social PhD program have got homework to do in the evening time …. @diamondlightsource.bsky.social @instruct-eric.bsky.social

Officer, I would like to report a murder

....merit....

SPACEtomo v1.3 is now available! What's new? - Lots of tools for setting up targets on non-lamella samples - Option for reacquisition of specific maps (e.g. after ice contamination cleaning) - Bug fixes and more robust Give it a try and let me know what is still missing! github.com/eisfabian/SP...

Happy to share the latest work from the Gonen lab! A collective effort to solve the structure of the lens membrane protein central to keeping our lenses transparent! Fluorescent targeted FIB milling, LCP and microED made this possible! www.nature.com/articles/s41...

"I, however, knew two hours before the first bombs exploded that the attack might be coming. The reason I knew this is that Pete Hegseth, the secretary of defense, had texted me the war plan at 11:44 a.m." 😱

Colorado…

This !!!! ⬇️⬇️⬇️

Amazing! A moving cover, just like in Harry Potter 🤩

ChimeraX can be used to find pockets and cavities in protein structures using the Find Cavities tool or kvfinder command. This feature is available in daily builds from 10 March 2025 or later.

Hell yeah

👀

NEW: Senator Marshall (R-KS) RUNS AWAY, fleeing his own town hall after being asked about DOGE firing Veterans. MAKE HIM GO VIRAL.

If you are in a position to push back on the Musk/DOGE wreckers who want to kill NIH, send us a message on Signal and we can look to get you advice on what to do.

We should perhaps consider doing the same on all the university science buildings

This is really smart by @sanders.senate.gov Go to republican districts and push voters to pressure their republican reps. www.commondreams.org/news/bernie-...

Striking gold!