How does hunger spur us to consume? @columbiauniversity.bsky.social scientists have found a brain area that drives mice to consume sweets, fats, salt & food. This finding may inform novel treatments for both overeating and undereating: zuckermaninstitute.columbia.edu/brain-dial-c...

Progress in the development of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40896196/ #cryoem

Brooklyn at sunset

Our latest paper on the development of an #ultrafast #laser #PhasePlate for #cryoET @fitzpatricklab.bsky.social @zuckermanbrain.bsky.social doi.org/10.1063/4.0000770

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators. www.nature.com/articles/s41...

Contrast by electron microscopy in thick biological specimens pubmed.ncbi.nlm.nih.gov/40857101/ #cryoem

Optimal tilt-increment for cryo-ET www.biorxiv.org/content/10.1101/2025.08.20.671201v1 #cryoem

Fourier-based multiple-slice reconstruction in cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40834650/ #cryoem

A massive genetic study in @Nature just identified why chronic pain hits some people harder than others. They discovered the first neuronal “pain transporter” - and it could unlock entirely new ways to treat pain. 🧵

ICYMI: New online: Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug

New "Mindscapes" exhibit @zuckermanbrain.bsky.social‬ featuring #cryoEM structures of #TMEM106B fibrils (doi.org/10.1016/j.cell.2022.02.026) and the ligand-bound human sweet receptor (doi.org/10.1016/j.cell.2025.04.021) from the Fitzpatrick (www.fitzpatrick-lab.org) and Zuker (www.zukerlab.com) labs

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Flexibility can be frustrating when processing a #cryoEM dataset. If your target has a wobbly domain, check out our new case study on FaNaC1 for continuous heterogeneity tips in #CryoSPARC! guide.cryosparc.com/processing-d...

New post! Our summer edition of What's new in biology: The first gonorrhea vaccination program, contact lenses that see infrared light, the protein behind sweet tastes, baby cured with gene therapy, gene-editing breakthroughs & new cholesterol drugs. Written by me and @nikomccarty.bsky.social

Happy to say that my Current Opinion in Structural Biology article came out today. I hope it helps to guide to you in using cryo-ET to make new biological discoveries! www.sciencedirect.com/science/arti...

Forward-looking preview by Bin Wu to our latest paper in Structure by @andrewnchang.bsky.social highlighting the power of #AI in #cryoEM of patient-derived samples: tinyurl.com/tu2fyvme Paper: tinyurl.com/3cycffzm Many thanks to @thorstenwagner.bsky.social @raunser-lab.bsky.social for #crYOLO

Ever wonder why SGD, which works so well for ab initio cryo-EM, never seems to get to high resolution on its own? Well, we have the answer: conditioning!

Proud to share our latest paper. doi.org/10.1016/j.cr... Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue. Structural biology in tissue is well and truly here! @rosfrankinst.bsky.social

New preprint from Daniel Du @fitzpatricklab.bsky.social shows #femtosecond light pulses can lens electron beams, promising higher contrast in #cryoET and better aberration correction in #cryoEM tinyurl.com/257sw7j9 Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture

An approach for coherent periodogram averaging of tilt-series data for improved contrast transfer function estimation 👉 buff.ly/hDgWZMA #cryoEM #StructuralBiology #CryoET

Structural basis of human uromodulin filament networks in uropathogen capture pubmed.ncbi.nlm.nih.gov/40345203/ #cryoem

>20 years ago, Zuker et al. discovered the mammalian sweet taste receptors Now, an exciting collaboration between our labs reveals the #cryoEM structure of the human sweet receptor-key to sweet taste perception-online @cp-cell.bsky.social! www.sciencedirect.com/science/article/pii/S0092867425004568

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

Nature research paper: Structural basis of lipid transfer by a bridge-like lipid-transfer protein https://go.nature.com/3Gn657r

I'm excited to share our lab's newest publication on the structure and pharmacology of human GABA transporter 3 @natcomms.nature.com. Much to our surprise, GAT3 adopts an inward-open conformation in complex with the (moderately) selective antagonist SNAP-5114. 💊 🧪 #CryoEM #OpenAccess rdcu.be/ei2KY

We have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples! Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵 #CryoET #TeamTomo www.biorxiv.org/content/10.1...

3/ Awardee @cmholab.bsky.social tackles the complex challenges of protein sequencing by leveraging microbial molecular machinery. Her work can revolutionize how we study protein function/structure & our understanding of biology🤯. 🥳Congrats Dr. Ho! @columbiauniversity.bsky.social 🙏#HFScout Ellen Yeh

Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/... @wehi-research.bsky.social @komanderlab.bsky.social

@science.org Cryo-EM reveals mechanisms of natural RNA multivalency | Science www.science.org/doi/10.1126/... #RNAsky #RNAbiology #Cryo-EM

Mindblowing new study by @raflynn5.bsky.social @bostonchildrens.bsky.social, cell surface RNA-binding proteins form nanoclusters with #glycoRNA and mediate cell-penetrating peptide entry into cells 👏 www.cell.com/cell/abstrac...

Happy to share this new #glycotime work with collaborator Tony Wyss-Coray, led by grad student Sophia Shi, mucin loss at the BBB is associated with aging and cognitive decline, can be reversed by restoration with with gene therapy. Aging is a mucinopathy! www.nature.com/articles/s41...

Endogenous structure of antimalarial target PfATP4 reveals new class of apicomplexan P-type ATPase modulators www.biorxiv.org/content/10.1101/2025.02.25.640208v1 #cryoem

In our first study, harnessing native Cx46/50 gap junctions, we provide the first atomic-level view of pH gating. Under acidic conditions, lipids infiltrate the pore, displacing the NT domain and reversibly closing the channel. www.biorxiv.org/content/10.1...

In @justinditrani.bsky.social final postdoc paper (with contributions from many others) we show that forming/purifying vesicles (here from M. smegmatis) allows structure det'n of *endogenous* membrane proteins in their *native lipid bilayer* 🧵 on methods & TB biology... www.pnas.org/doi/full/10....

Synaptotoxic forms of amyloid-b and a-synuclein act through a common pathway. https://www.biorxiv.org/content/10.1101/2025.02.13.638131v1

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬 The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy www.biorxiv.org/content/10.1...

New preprint from the MemTraS team ! www.biorxiv.org/content/10.1... Great collaboration with @fronzeslab.bsky.social and Vladan Lucic to determine the first cryo electron tomography images of dopamine "synapses" extracted from adult mouse brain.

New paper from our lab @naturecomms.bsky.social! We reveal the dynamics and mechanism of target DNA traversal in #CRISPR Cas12a, a conundrum in the field! nature.com/articles/s41... #compchem We thank the amazing #HPC resources of PSC #Anton2 and SDSC

Excited to see work led by @autophazeke.bsky.social, @minghaochen.bsky.social, Thanh Nguyen, and Ainara Claveras out in @science.org today, the culmination of a 15-year project to understand structurally how the synthesis of the critical lipid of autophagy, PI3P, is regulated. tinyurl.com/ywvjh8u4

It’s incredibly hard to study lipids in biological membranes on the nanoscale. You need near-perfect information on both membrane ultrastructure and lipid density, which usually isn’t attainable. Lipid-CLEM brought to you by @mathilda95.bsky.social changes that. www.biorxiv.org/content/10.1...

Proud to share: "Ensemble docking for intrinsically disordered proteins" from Dartmouth undergrad Anjali Dhar 24' and grad student Tommy Sisk. We present ensemble docking strategies for IDPs that, remarkably, seem to work! www.biorxiv.org/content/10.1... Code: github.com/paulrobustel...

Structure of a zoonotic H5N1 hemagglutinin reveals a receptor-binding site occupied by an auto-glycan pubmed.ncbi.nlm.nih.gov/39884273/ #cryoem

Researchers used AlphaFold alone (no wet-lab experiments; no cryo-EM etc.) to build a structural model and predict how a huge complex in cells, made of 17 proteins, spans across the bacterial cell envelope to transduce force for motility. Can anyone tell me if this is a first?

I'd like to draw your attention to this truly excellent paper from the Taraska lab on cryoET of plasma membrane associated proteins. Everyone who is thinking about probes in the cryoET space should also see what they could do with ferritag (fig 6) www.nature.com/articles/s41...

It was a pleasure to host Professor Wolfgang Baumeister and share the latest results on developing an #ultrafast #laser #phaseplate for #cryoEM and #cryoET from @fitzpatricklab.bsky.social @zuckermanbrain.bsky.social

Kexin Zhang et al.: A new statistical metric for robust target detection in cryo-EM using 2D template matching ##3DReconstructionAndImageProcessing##SingleParticleCryoEM##Imaging@umassboston...##IUCrhttps://journals.iucr.org/paper?S2052252524011771

Paper of interest for the #CryoET folks. www.nature.com/articles/s41...

For all #cryoEM practitioners in #NYC, @fitzpatricklab.bsky.social is excited to host Prof. Wolfgang Baumeister next Thursday with attendance open to all and no registration necessary. Hope to see you there!

Excited to present our lab's new work on GPCR antagonism: Contrary to popular belief, we propose that inverse agonists can bind to and act via GPCR:G protein complexes. We show this at KOR, through cellular assays, radioligand binding and cryoEM studies. A thread. 💊 🧪 www.nature.com/articles/s41...

This was a great collaboration and culmination of a huge amount of work including our first attempt to join #teamtomo. @ruizhangmt.bsky.social expertly summarizes the highlights below: