@fitzpatricklab.bsky.social
📤 698
📥 2762
📝 17
pinned post!
New preprint from Daniel Du
@fitzpatricklab.bsky.social
shows
#femtosecond
light pulses can lens electron beams, promising higher contrast in
#cryoET
and better aberration correction in
#cryoEM
tinyurl.com/257sw7j9
Experiments made possible by support from
@cziscience.bsky.social
#ImagingTheFuture
8 months ago
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reposted by
Structura Biotechnology
18 days ago
🚀 CryoSPARC v5.0 BETA is here! We’re excited to deploy another major
#CryoSPARC
release to help enable and accelerate
#cryoEM
data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread! Full changelog:
cryosparc.com/updates/v5.0.0
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Frozen Bryant Park fountain in snowy NYC!
18 days ago
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reposted by
Science X / Phys.org
28 days ago
A combined approach using light microscopy and ion beam milling enhances the precision of cryoET sample preparation, enabling more accurate targeting of small, rare structures within cells.
doi.org/hbkb94
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Tightening the focus of subcellular snapshots: Combined approach yields better cell slices for cryoET imaging
Taking images of tiny structures within cells is tricky business. One technique, cryogenic electron tomography (cryoET), shoots electrons through a frozen sample.
https://phys.org/news/2026-01-tightening-focus-subcellular-snapshots-combined.html?utm_source=bsky.app&utm_medium=social&utm_campaign=v2
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reposted by
cryoEM papers
about 1 month ago
Reversible lipid-mediated pH-gating of connexin-46/50 by cryo-EM
pubmed.ncbi.nlm.nih.gov/41526355/
#cryoem
0
3
2
reposted by
bioRxivpreprint
2 months ago
Amyloid-mediated RNA uptake by sperm for embryonic delivery
https://www.biorxiv.org/content/10.64898/2025.12.10.693493v1
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2
1
reposted by
Andrew Ellisdon
about 1 month ago
Excited to share our latest research, out today in Cell (
@cellpress.bsky.social
) Using cryo-EM and cell-based studies, we reveal how large nutrient sensing protein complexes form at the lysosomal membrane to turn off mTORC1 and halt anabolic signalling. Read the paper here:
doi.org/10.1016/j.ce...
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Another member of the Fitzpatrick/Shapiro quad
@zuckermanbrain.bsky.social
embarks on setting up his lab! If you wish to use
#cryoEM
#cryoET
to study how viruses enter & evade the immune system, & design antibody-based vaccines, check out the Roark lab:
isb.med.upenn.edu/research/cryoem-and-cryoet
about 1 month ago
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4
0
reposted by
Yamuna Krishnan
about 2 months ago
Organelles do NOT have a single uniform pH. And if you think they must, because “protons diffuse fast,” this paper is for you. A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
add a skeleton here at some point
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reposted by
Pawel Burkhardt
about 2 months ago
Thrilled to see our review article "The evolutionary origins of synaptic proteins" highlighted on the cover of Nature Reviews Neuroscience 🤩.
www.nature.com/articles/s41...
@msarscentre.bsky.social
🧠✨🧬🌊🪼🧽
add a skeleton here at some point
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reposted by
Kihara Laboratory
2 months ago
A quick tutorial on the DAQ structure validation score for protein models derived from cryo-EM. DAQ evaluates amino acid–level accuracy in your model. Consider including a DAQ validation report in your next publication!
www.youtube.com/watch?v=YRAT...
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DAQ-Score: Automatic AI-based Cryo-EM Structure Model Validation!
YouTube video by Kihara Bioinformatics Lab
https://www.youtube.com/watch?v=YRAT98tQx0Q
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reposted by
Roger Castells
2 months ago
I’m very happy (and grateful!) to share that I’m featured in a Nature Cancer Viewpoint about starting my lab at CNIO. In the Lab (
www.rcglab.com
) we’re combining AI, protein design and structural biology to study protein structures and design novel protein therapeutics for cancer. 🔬
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reposted by
Rémi Fronzes
2 months ago
🔬 Just out in @NatureComms! Using cryo-CLEM and cryo-ET, we reveal the unique ultrastructure of dopaminergic varicosities in the striatum. Amazing collaboration with
@davip-bdx.bsky.social
and Etienne Herzog
@iins-bordeaux.bsky.social
Congratulations to Paul and Robin for this fantastic work! 🎉
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Cryo-correlative light and electron tomography of dopaminergic axonal varicosities reveals non-synaptic modulation of cortico-striatal synapses - Nature Communications
The basic features of dopamine release sites are still largely unknown. Here, the authors determine the ultrastructure of fluorescent dopaminergic and glutamatergic synaptosomes in mouse striatum usin...
https://www.nature.com/articles/s41467-025-66355-x
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reposted by
Kihara Laboratory
2 months ago
December Update of DAQ-Score DB! Quality evaluations of protein models from cryo-EM. Now includes evaluation of 258,956 protein chains. Check at
daqdb.kiharalab.org
It's very easy to compute DAQ for your protein model. Add the validation result in your publication:
em.kiharalab.org/algorithm/da...
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reposted by
Ben Barad
2 months ago
Holy cow the Zhou lab at UCLA is cooking with Isonet 2 -
www.biorxiv.org/content/10.6...
Averaging-free direct visualization of ribosome translational state!
#teamtomo
#cryoet
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36
reposted by
Chi-Min Ho
2 months ago
😍 Zhou Lab on fire!!! 🔥🔥🔥
#cryoet
#teamtomo
add a skeleton here at some point
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3
reposted by
Sven T. Stripp
2 months ago
Love the caption of Fig. 3 in this impressive preprint: "A Goodsell-esque 3D orthogonal rendering of an IsoNet2-processed FIB-milled tomogram of C. reinhardtii"
doi.org/10.64898/202...
Since I am no expert, what does the cryoET community think of IsoNet?
@cellarchlab.com
doi.org/10.1038/s414...
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reposted by
cryoEM papers
2 months ago
IsoNet2 determines cellular structures at submolecular resolution without averaging
www.biorxiv.org/content/10.64898/2025.12.09.693325v1
#cryoem
0
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8
Many thanks to
@cellpress.bsky.social
for highlighting our
#cryoEM
research:
cell.com/structure/fu...
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Our authors in 2025
As 2025 comes to an end, we want to highlight some of the rising young faculty members who have published their exciting work from different areas of structural biology in Structure this year. We have...
https://cell.com/structure/fulltext/S0969-2126(25)00441-1
2 months ago
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0
reposted by
Richard Held
2 months ago
I wrote a bit about practical considerations for using
#cryo-ET
labels and some things to work on if we want to find any protein in any cell.
authors.elsevier.com/a/1mA6h,LqAr...
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https://authors.elsevier.com/a/1mA6h,LqArXr1S
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15
4
reposted by
Alan Brown
3 months ago
Delighted to have contributed to this paper and amazing resource. In-silico protein-protein interaction screening has huge promise for generating novel hypotheses and building models into low-res
#cryo-EM
and
#teamtomo
maps.
add a skeleton here at some point
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15
3
reposted by
cryoEM papers
3 months ago
Seeing is believing-Plasmodium falciparum translation in action
pubmed.ncbi.nlm.nih.gov/41202797/
#cryoem
0
7
4
reposted by
Cornelius Gati
3 months ago
Excited to share our latest
@nature.com
: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇
www.nature.com/articles/s41...
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reposted by
Harry Low’s Lab
3 months ago
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (
www.thelowlab.org
) at Imperial, London, working on the structure and mechanism of bacterial secretion systems. For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
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reposted by
Chi-Min Ho
4 months ago
Learn how our hunt for the native structure of top antimalarial target PfATP4🧂led to the discovery of PfABP, an unknown essential binding partner, out now!
@natcomms.nature.com
👉
rdcu.be/eLRlH
🦠🔬❄️ A team effort led by
@mehsehret.bsky.social
& Anurag Shukla
@akhilvaidya.bsky.social
!
#cryoEM
#malaria
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Endogenous structure of antimalarial target PfATP4 reveals an apicomplexan-specific P-type ATPase modulator
Nature Communications - Here, the authors present the 3.7 Å cryoEM structure of native sodium efflux pump PfATP4 from Plasmodium falciparum, revealing a bound protein that they term...
https://rdcu.be/eLRlH
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reposted by
Sternberg Lab
4 months ago
1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
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Antiviral reverse transcriptases reveal the evolutionary origin of telomerase
Defense-associated reverse transcriptases (DRTs) employ diverse and distinctive mechanisms of cDNA synthesis to protect bacteria against viral infection. However, much of DRT family diversity remains ...
https://www.biorxiv.org/content/10.1101/2025.10.16.682844v1
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reposted by
Giuseppe Di Caprio
4 months ago
A beautiful piece of work from
@ruixuan3.bsky.social
and
@supgokul.bsky.social
presenting a light-based slicing method using a photosensitive hydrogel to image large biological samples layer by layer, preserving fine structure for full 3D reconstructions.
add a skeleton here at some point
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As ever, beautiful
#cryoEM
work from the lab of Hong Zhou with field-defining insights using
#cryoET
: “Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
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“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling
Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...
https://www.science.org/doi/10.1126/science.ads7954
4 months ago
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reposted by
Piotr Draczkowski
4 months ago
If you're curious about how cells quickly eliminate misfolded proteins produced under proteotoxic stress or mitotic substrate during cell cycle progression, check out our latest article in Nature Communications:
doi.org/10.1038/s414...
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Structural basis of K11/K48-branched ubiquitin chain recognition by the human 26S proteasome - Nature Communications
K11/K48 branched ubiquitin chains regulate protein degradation and cell cycle progression. Here, the authors report the structural basis of how such a branched ubiquitin chain is recognized by the hum...
https://doi.org/10.1038/s41467-025-64719-x
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reposted by
joe dobbs
4 months ago
Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we
@embl.org
were able to describe bacterial populations with in-cell
#cryoET
. And there’s a surprise at the end 🕵️
www.biorxiv.org/content/10.1...
#teamtomo
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39
reposted by
André Nadler
4 months ago
Lipid imaging on the cover of
@nature.com
! Great times for lipid cell biology indeed. And a fantastic recognition of all the hard work by the team, especially Juan M. Iglesias-Artola and Kristin Böhlig (who made the cover). Link to article:
www.nature.com/articles/s41...
9
165
43
reposted by
cryoEM papers
4 months ago
Ballistic Microscopy (BaM)
www.biorxiv.org/content/10.1101/2025.10.07.681030v1
#cryoem
0
7
3
reposted by
eLife
4 months ago
High-resolution
#CryoET
provides ‘compelling’ evidence for the true 3D architecture of glutamatergic synapses: a complex ‘synaptoplasm’ rather than the textbook postsynaptic density.
buff.ly/hOYKjHH
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reposted by
Columbia University's Zuckerman Institute
5 months ago
How does hunger spur us to consume?
@columbiauniversity.bsky.social
scientists have found a brain area that drives mice to consume sweets, fats, salt & food. This finding may inform novel treatments for both overeating and undereating:
zuckermaninstitute.columbia.edu/brain-dial-c...
1
3
2
reposted by
cryoEM papers
5 months ago
Progress in the development of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography
pubmed.ncbi.nlm.nih.gov/40896196/
#cryoem
0
3
2
Brooklyn at sunset
6 months ago
0
6
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Our latest paper on the development of an
#ultrafast
#laser
#PhasePlate
for
#cryoET
@fitzpatricklab.bsky.social
@zuckermanbrain.bsky.social
doi.org/10.1063/4.0000770
6 months ago
0
17
2
reposted by
Martin Pacesa
6 months ago
Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian,
@sokrypton.org
, Bruno and many other amazing lab members and collaborators.
www.nature.com/articles/s41...
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120
reposted by
cryoEM papers
6 months ago
Contrast by electron microscopy in thick biological specimens
pubmed.ncbi.nlm.nih.gov/40857101/
#cryoem
0
4
3
reposted by
cryoEM papers
6 months ago
Optimal tilt-increment for cryo-ET
www.biorxiv.org/content/10.1101/2025.08.20.671201v1
#cryoem
0
8
3
reposted by
cryoEM papers
6 months ago
Fourier-based multiple-slice reconstruction in cryo-electron tomography
pubmed.ncbi.nlm.nih.gov/40834650/
#cryoem
0
4
2
reposted by
Dr. Dominic Ng
6 months ago
A massive genetic study in @Nature just identified why chronic pain hits some people harder than others. They discovered the first neuronal “pain transporter” - and it could unlock entirely new ways to treat pain. 🧵
1
1
1
reposted by
Nature Structural & Molecular Biology
6 months ago
ICYMI: New online: Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug
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Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug
Nature Structural & Molecular Biology, Published online: 18 August 2025; doi:10.1038/s41594-025-01632-3Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation elongation cycle and its perturbation by cabamiquine, a potent antimalarial drug candidate.
https://go.nature.com/3UysanG
0
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New "Mindscapes" exhibit
@zuckermanbrain.bsky.social
featuring
#cryoEM
structures of
#TMEM106B
fibrils (
doi.org/10.1016/j.cell.2022.02.026
) and the ligand-bound human sweet receptor (
doi.org/10.1016/j.cell.2025.04.021
) from the Fitzpatrick (www.fitzpatrick-lab.org) and Zuker (
www.zukerlab.com
) labs
6 months ago
0
8
0
reposted by
Chi-Min Ho
6 months ago
Our
#CryoET
story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out
@natsmb.nature.com
🥳
rdcu.be/eBbrH
A massive team effort led by
@leonieanton.bsky.social
Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman!
#TeamTomo
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Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug
Nature Structural & Molecular Biology - Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation...
https://rdcu.be/eBbrH
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40
reposted by
Structura Biotechnology
6 months ago
Flexibility can be frustrating when processing a
#cryoEM
dataset. If your target has a wobbly domain, check out our new case study on FaNaC1 for continuous heterogeneity tips in
#CryoSPARC
!
guide.cryosparc.com/processing-d...
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25
5
reposted by
Saloni
7 months ago
New post! Our summer edition of What's new in biology: The first gonorrhea vaccination program, contact lenses that see infrared light, the protein behind sweet tastes, baby cured with gene therapy, gene-editing breakthroughs & new cholesterol drugs. Written by me and
@nikomccarty.bsky.social
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What's new in biology: summer 2025
The first gonorrhea vaccination program, contact lenses that see infrared light, the protein behind sweet tastes, a baby cured with gene therapy, and more
https://www.worksinprogress.news/p/whats-new-in-biology
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reposted by
Will Wan
7 months ago
Happy to say that my Current Opinion in Structural Biology article came out today. I hope it helps to guide to you in using cryo-ET to make new biological discoveries!
www.sciencedirect.com/science/arti...
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A practical look at cryo-electron tomography image processing: Key considerations for new biological discoveries
Cryo-electron tomography (cryo-ET) enables 3D visualization of complex biological environments without the need for purification, thereby preserving t…
https://www.sciencedirect.com/science/article/pii/S0959440X25001344
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Forward-looking preview by Bin Wu to our latest paper in Structure by
@andrewnchang.bsky.social
highlighting the power of
#AI
in
#cryoEM
of patient-derived samples:
tinyurl.com/tu2fyvme
Paper:
tinyurl.com/3cycffzm
Many thanks to
@thorstenwagner.bsky.social
@raunser-lab.bsky.social
for
#crYOLO
8 months ago
0
8
2
reposted by
Marcus Brubaker
8 months ago
Ever wonder why SGD, which works so well for ab initio cryo-EM, never seems to get to high resolution on its own? Well, we have the answer: conditioning!
add a skeleton here at some point
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reposted by
Michael Grange
8 months ago
Proud to share our latest paper.
doi.org/10.1016/j.cr...
Through the dedication of
@glynnca.bsky.social
and
@cryingem.bsky.social
we report a thorough method to image molecular organisation within hippocampus tissue. Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
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