Seeing is believing-Plasmodium falciparum translation in action pubmed.ncbi.nlm.nih.gov/41202797/ #cryoem

Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...

Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems. For more details and to apply please see www.imperial.ac.uk/jobs/search-...

Learn how our hunt for the native structure of top antimalarial target PfATP4🧂led to the discovery of PfABP, an unknown essential binding partner, out now! @natcomms.nature.com 👉 rdcu.be/eLRlH 🦠🔬❄️ A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria

1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria. www.biorxiv.org/content/10.1...

A beautiful piece of work from @ruixuan3.bsky.social and @supgokul.bsky.social presenting a light-based slicing method using a photosensitive hydrogel to image large biological samples layer by layer, preserving fine structure for full 3D reconstructions.

As ever, beautiful #cryoEM work from the lab of Hong Zhou with field-defining insights using #cryoET: “Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science www.science.org/doi/10.1126/...

If you're curious about how cells quickly eliminate misfolded proteins produced under proteotoxic stress or mitotic substrate during cell cycle progression, check out our latest article in Nature Communications: doi.org/10.1038/s414...

Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️ www.biorxiv.org/content/10.1... #teamtomo

Lipid imaging on the cover of @nature.com! Great times for lipid cell biology indeed. And a fantastic recognition of all the hard work by the team, especially Juan M. Iglesias-Artola and Kristin Böhlig (who made the cover). Link to article: www.nature.com/articles/s41...

Ballistic Microscopy (BaM) www.biorxiv.org/content/10.1101/2025.10.07.681030v1 #cryoem

High-resolution #CryoET provides ‘compelling’ evidence for the true 3D architecture of glutamatergic synapses: a complex ‘synaptoplasm’ rather than the textbook postsynaptic density. buff.ly/hOYKjHH

How does hunger spur us to consume? @columbiauniversity.bsky.social scientists have found a brain area that drives mice to consume sweets, fats, salt & food. This finding may inform novel treatments for both overeating and undereating: zuckermaninstitute.columbia.edu/brain-dial-c...

Progress in the development of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40896196/ #cryoem

Brooklyn at sunset

Our latest paper on the development of an #ultrafast #laser #PhasePlate for #cryoET @fitzpatricklab.bsky.social @zuckermanbrain.bsky.social doi.org/10.1063/4.0000770

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators. www.nature.com/articles/s41...

Contrast by electron microscopy in thick biological specimens pubmed.ncbi.nlm.nih.gov/40857101/ #cryoem

Optimal tilt-increment for cryo-ET www.biorxiv.org/content/10.1101/2025.08.20.671201v1 #cryoem

Fourier-based multiple-slice reconstruction in cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40834650/ #cryoem

A massive genetic study in @Nature just identified why chronic pain hits some people harder than others. They discovered the first neuronal “pain transporter” - and it could unlock entirely new ways to treat pain. 🧵

ICYMI: New online: Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug

New "Mindscapes" exhibit @zuckermanbrain.bsky.social‬ featuring #cryoEM structures of #TMEM106B fibrils (doi.org/10.1016/j.cell.2022.02.026) and the ligand-bound human sweet receptor (doi.org/10.1016/j.cell.2025.04.021) from the Fitzpatrick (www.fitzpatrick-lab.org) and Zuker (www.zukerlab.com) labs

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Flexibility can be frustrating when processing a #cryoEM dataset. If your target has a wobbly domain, check out our new case study on FaNaC1 for continuous heterogeneity tips in #CryoSPARC! guide.cryosparc.com/processing-d...

New post! Our summer edition of What's new in biology: The first gonorrhea vaccination program, contact lenses that see infrared light, the protein behind sweet tastes, baby cured with gene therapy, gene-editing breakthroughs & new cholesterol drugs. Written by me and @nikomccarty.bsky.social

Happy to say that my Current Opinion in Structural Biology article came out today. I hope it helps to guide to you in using cryo-ET to make new biological discoveries! www.sciencedirect.com/science/arti...

Forward-looking preview by Bin Wu to our latest paper in Structure by @andrewnchang.bsky.social highlighting the power of #AI in #cryoEM of patient-derived samples: tinyurl.com/tu2fyvme Paper: tinyurl.com/3cycffzm Many thanks to @thorstenwagner.bsky.social @raunser-lab.bsky.social for #crYOLO

Ever wonder why SGD, which works so well for ab initio cryo-EM, never seems to get to high resolution on its own? Well, we have the answer: conditioning!

Proud to share our latest paper. doi.org/10.1016/j.cr... Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue. Structural biology in tissue is well and truly here! @rosfrankinst.bsky.social

New preprint from Daniel Du @fitzpatricklab.bsky.social shows #femtosecond light pulses can lens electron beams, promising higher contrast in #cryoET and better aberration correction in #cryoEM tinyurl.com/257sw7j9 Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture

An approach for coherent periodogram averaging of tilt-series data for improved contrast transfer function estimation 👉 buff.ly/hDgWZMA #cryoEM #StructuralBiology #CryoET

Structural basis of human uromodulin filament networks in uropathogen capture pubmed.ncbi.nlm.nih.gov/40345203/ #cryoem

>20 years ago, Zuker et al. discovered the mammalian sweet taste receptors Now, an exciting collaboration between our labs reveals the #cryoEM structure of the human sweet receptor-key to sweet taste perception-online @cp-cell.bsky.social! www.sciencedirect.com/science/article/pii/S0092867425004568

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

Nature research paper: Structural basis of lipid transfer by a bridge-like lipid-transfer protein https://go.nature.com/3Gn657r

I'm excited to share our lab's newest publication on the structure and pharmacology of human GABA transporter 3 @natcomms.nature.com. Much to our surprise, GAT3 adopts an inward-open conformation in complex with the (moderately) selective antagonist SNAP-5114. 💊 🧪 #CryoEM #OpenAccess rdcu.be/ei2KY

We have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples! Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵 #CryoET #TeamTomo www.biorxiv.org/content/10.1...

3/ Awardee @cmholab.bsky.social tackles the complex challenges of protein sequencing by leveraging microbial molecular machinery. Her work can revolutionize how we study protein function/structure & our understanding of biology🤯. 🥳Congrats Dr. Ho! @columbiauniversity.bsky.social 🙏#HFScout Ellen Yeh

Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/... @wehi-research.bsky.social @komanderlab.bsky.social

@science.org Cryo-EM reveals mechanisms of natural RNA multivalency | Science www.science.org/doi/10.1126/... #RNAsky #RNAbiology #Cryo-EM

Mindblowing new study by @raflynn5.bsky.social @bostonchildrens.bsky.social, cell surface RNA-binding proteins form nanoclusters with #glycoRNA and mediate cell-penetrating peptide entry into cells 👏 www.cell.com/cell/abstrac...

Happy to share this new #glycotime work with collaborator Tony Wyss-Coray, led by grad student Sophia Shi, mucin loss at the BBB is associated with aging and cognitive decline, can be reversed by restoration with with gene therapy. Aging is a mucinopathy! www.nature.com/articles/s41...

Endogenous structure of antimalarial target PfATP4 reveals new class of apicomplexan P-type ATPase modulators www.biorxiv.org/content/10.1101/2025.02.25.640208v1 #cryoem

In our first study, harnessing native Cx46/50 gap junctions, we provide the first atomic-level view of pH gating. Under acidic conditions, lipids infiltrate the pore, displacing the NT domain and reversibly closing the channel. www.biorxiv.org/content/10.1...

In @justinditrani.bsky.social final postdoc paper (with contributions from many others) we show that forming/purifying vesicles (here from M. smegmatis) allows structure det'n of *endogenous* membrane proteins in their *native lipid bilayer* 🧵 on methods & TB biology... www.pnas.org/doi/full/10....

Synaptotoxic forms of amyloid-b and a-synuclein act through a common pathway. https://www.biorxiv.org/content/10.1101/2025.02.13.638131v1

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬 The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy www.biorxiv.org/content/10.1...

New preprint from the MemTraS team ! www.biorxiv.org/content/10.1... Great collaboration with @fronzeslab.bsky.social and Vladan Lucic to determine the first cryo electron tomography images of dopamine "synapses" extracted from adult mouse brain.

New paper from our lab @naturecomms.bsky.social! We reveal the dynamics and mechanism of target DNA traversal in #CRISPR Cas12a, a conundrum in the field! nature.com/articles/s41... #compchem We thank the amazing #HPC resources of PSC #Anton2 and SDSC