Prohibitins aren’t hanging out just anywhere in the membrane — they prefer the 💁‍♀️💎✨VIP section💅🪩🥂 Lab all-star @mmedina300kv.bsky.social maps these microdomains using cryo-ET + surface morphometrics. s/o to #teamtomo #cryoET co-authors @hamid13r.bsky.social @attychang.bsky.social @baradlab.com

IFL Science: “Lupus Linked To Virus That Over 95 Percent Of Us Carry – And Now We Finally Know How” "We think it applies to 100% of lupus” cases." www.iflscience.com/lupus-linked...

MPI–Weizmann Postdoc (4-year, co-mentored) Together with Ori Avinoam (Weizmann), we’re recruiting a postdoc to uncover membrane remodelling mechanisms in muscle development using cryo-EM of recombinant proteins, and cell imaging/CLEM. Apply here by 21.11.25 forms.gle/ZtDWUjcTBu4d...

Excited for Adam Klivans and my other colleagues at UT working in the AI space!

An Asgard archaeon with internal membrane compartments Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors! www.biorxiv.org/content/10.1...

Glad to see our antibody design paper finally out in @nature.com (and congrats to lead authors and everyone involved )! At Xaira we are excited about pushing antibody design further to bind harder targets and make drugs for unmet medical needs. Paper: www.nature.com/articles/s41...

Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️ Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...

Glushkova, Böhm, & Beck @maxplanck.de develop a publicly available computational method to measure the thickness of biological membranes in cryo-electron tomograms. Analysis of algae & human cells reveals systematic membrane thickness variations within & across organelles rupress.org/jcb/article/...

Systematic membrane thickness variation across cellular organelles revealed by cryo-ET pubmed.ncbi.nlm.nih.gov/41186573/ #cryoem

Lessons learned from a Kaggle challenge for particle picking in cryo-electron tomography [new] Kaggle cryo-ET picking: Over-pick tol, aug, & annot key.

Just completed 100 miles at Austin rolling through Texas Hill Country. The event is 2025 LiveStrong Challenge to support cancer research. Feeling blessed!

We are looking for a computational postdoc to work with us on new optimisation algorithms to make #RELION even better. Join our bubbly team at the @mrclmb.bsky.social in Cambridge, UK. 🤗 RTs appreciated. mrc.tal.net/vx/appcentre...

Ever seen a pumpkin under a microscope? 🎃🔬 This Halloween, Raymond Wightman & Daniel Sparrow used @slcuplants.bsky.social’s powerful imaging tools to reveal: 🔬 Skin detail with the #Keyence microscope 💡 Beta-carotene via #Raman spectroscopy ❄️ Flash-frozen oval cells via cryo-SEM #HappyHalloween

New lab preprint! @zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes 🧵 tinyurl.com/ukny4ptz

The biophysics class I taught recently did kinesin walks hand-over-hand model (2004, Science), with reference to the inchworm model (2002,Science). Students discussed danced to mimic the two models, super fun! Thanks to the shortage of giant squid due to meteorological disturbance El Niño in 1983!

Another undergraduate paper discussion today! This time we are doing Ron Vale's 1985 kinesin trifecta. Focusing on [1] & [3]. 🧪🔬 [1] www.sciencedirect.com/science/arti... [2] www.sciencedirect.com/science/arti... [3] www.sciencedirect.com/science/arti... [4] www.ibiology.org/cell-biology...

Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧

“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science www.science.org/doi/10.1126/...

Excited to share a new chapter of my scientific journey in Chris Russo's group — developing a way to freeze proteins faster than they diffuse, paving the way for cryo-EM without air–water interface damage.

During these uncertain times, I’m very happy to see that my institution, @scripps.edu has an open tenure-track Assistant Professor position. Any field in Chemistry or Biology is welcome. I’d especially love to see fellow neuroscientists apply. Please repost! apply.interfolio.com/174756

Our home department of Molecular Biology & Biochemistry at UC Irvine is looking to hire a new tenure-track assistant professor in the broad area of structural biology. Come be our colleague! recruit.ap.uci.edu/JPF09887 Please apply and/or share this post.

Hey folks! I got a tattoo of my beloved PIEZO ion channel on my arm! Now I can show how they activate (flatten) under pressure by flexing—Will add a bit of performance art to my science talks 🧪🧠.

🚀 Introducing fully automated data processing for repeat-target #cryoEM. Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention. Preprint: www.biorxiv.org/content/10.1...

Yikes! In this paper the AlphaFold model of human plasminogen (PDB-8uq6) has been refined into noise (EMD-42462) using Phenix & ISOLDE. For some reason the authors even interpret side-chain interactions: #cryoEM

possibly stupid question - does anyone in #teamtomo or #cryoem recognize these little stacks (from EMPIAR-12549, U2OS cell lamellae)? The green circle is 300Å, and the spacing between the layers is ~50Å. The fourth, bell-shaped thing is something else, also not sure of that one..

It was wonderful to have Bijun with us @mpi-mp-potsdam.bsky.social & @incavirus.bsky.social to finally "use our microscope to its full potential" as our core-facility put it! 🔬 Congrats to all, esp. @bijuntang.bsky.social and @xanderjones.bsky.social ! Super happy to be a part of this great story!

I was tremendously lucky to be in @xanderjones.bsky.social's lab to watch @bijuntang.bsky.social move from technician to PhD to Postdoc and produce this magnum opus. #SalicS1 is the just reward for years of brilliance and hard work.Well done Bijun, Xander, Jing, and everyone involved! #SalicylicAcid

Really cool story on a FRET biosensor by Bijun Tang @bijuntang.bsky.social and an amazing team, now published @science.org I’m so proud of your PhD work at @slcuplants.bsky.social Below is the lockdown time in 2020 when you spent long hours screening your FRET biosensor, now known as SalicS1!

I'm honored to receive a MacArthur Fellowship. As always, I extend my sincerest thanks to all my wonderful mentors, colleagues, collaborators, admins, and trainees who make this work possible. #MacFellow

The mechanistic basis of cargo selection during Golgi maturation. From Rebecca Taylor, @katciazynska.bsky.social, @jggkaufman.bsky.social & @grigorytagiltsev.bsky.social at @mpibiochem.bsky.social with David Owen and Sean Munro's group @cellbiol-mrclmb.bsky.social | www.science.org/doi/10.1126/...

Very excited to announce that my first, first author paper, is now on BioRxiv! In this paper we unambiguously show #COPII coated vesicles in unperturbed human cells for the very first time!! Make my day and check it out! www.biorxiv.org/content/10.1... #Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬

We’re hosting the @embo.org Practical Course on In-situ structural biology by CryoFIB and CryoET in April 2026! 🧬❄️ You can expect hands-on training, cutting-edge methods, and a chance to work alongside leading experts in structural biology. 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...

Helical Reconstruction of Amyloids in cryoSPARC? We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social. Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...

Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup). A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.

Check out our new preprint from the @landerlab.bsky.social, Stagg-Lab and Cianfrocco-Lab: CryoSift - An accessible and automated CNN-driven tool for cryo-EM 2D class selection www.biorxiv.org/content/10.1... Happy testing: cryosift.org #cryoEM #CNN

⚠️ Half of commercial antibodies miss their target! So how do you pick the right one for your experiment? Here’s the solution 👉 Antibody characterization data (all tested in KO cells) are now available at: 🔗 onlygoodantibodies.co.uk Tested by @ycharos.bsky.social & @oga-community.bsky.social

🌟 Thrilled to share that my lab’s first publication is now out in @natmetabolism.nature.com. Congrats to the team 🎉 🧠 We discovered that neurons use endogenous fatty acids as an energy fuel, challenging long-standing models of sugars being the exclusive energy source for neurons. 1/3

Huntingtin binds and bundles F-actin! Thrilled to team up with the Humbert (Sorbonne Université) and Song (KAIST) labs on this discovery. We had a blast doing cryo-ET on this unexpected complex — and now so many new questions lie ahead! #TeamTomo #CryoET #HTT #actin doi.org/10.1126/scia...

Cool people, cool machines - and the burning question: what barber grooms those beards to perfection?

Congratulations, Yifan, for the Anatrace Award! Yifan’s work on the TRPV1 structure with David Julius helped launch the Resolution Revolution in cryo-EM. Not just in membrane proteins—hardly any area of biophysics today has remained untouched by the techniques Yifan pioneered.

#Training 👨‍🏫 🧑‍🏫 #CryoEM ❄️ 🔬 #Science 🧬 🦠 #Education 📚 Huge shoutout to our SPoCs for making this a grand success! 👏 Marzia Miletto Marcelo Alexandre de Farias Omar Davulcu

I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪

We have a preprint of a book chapter for you: Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles. We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography zenodo.org/records/1717...

These results are truly impressive! But I do worry that #cryoEM is moving into a phase where progress is made by optimizing parameter settings for black box programs. The devil is often in the details, and I am not sure how this could be implemented in #relion, as stated, without more info.

CEMOVIS revisited! 🙂

Sofia Lövestam's vault structure is now online in Structure @cellpress.bsky.social. 🥳 How beautifully explorative is #cryoEM! We never planned to do this. The vaults just came down with #amyloids from human brain tissue, and Sofia decided to have a closer look. www.cell.com/structure/fu...

I have been reading this super work ⬇️ with great interest and much enthusiasm as it discusses Ab recognition of viral glycosylation, but I don't understand how terminal GlcNAc can be a viral 'feature' as it corresponds to a stumpy human N-glycan... 1/3 #glycotime www.science.org/doi/10.1126/...

Happy to share the latest work from the lab, led by @mudgal17.bsky.social‬, in collaboration with the Weis lab @ethzurich.bsky.social. How do nuclear membranes fuse during NPC assembly? We answer this question in our latest work, where we identify a new mechanism for membrane fusion… (1/13)

AI for Cryo-ET 🌟 Our new paper presents a deep-learning framework that cleans up faulty tilt series in a more automated, precise and powerful way! 💪 Read more here: www.sciencedirect.com/science/arti...

Fantastic work by the group of @matteoall.bsky.social : a SUN5:Nesprin3 complex at the head:tail junction of sperm cells. 🤯 #teamtomo www.biorxiv.org/content/10.1...