Interplay Between Protein-RNA Binding and Phase Separation Drives Emergent Behavior in RNP Condensates https://www.biorxiv.org/content/10.64898/2026.06.25.734509v1

Online Now: Translational activation: An unforeseen function of RNP biomolecular condensates Online now:

Getting lost in technical nitpicking was not enough to replicate what the worst human reviewers can do. Happy to announce a decisive further step: QED can now also spit nonsense on manuscript impact

Whether condensates have real biological functions has long been debated. Our new Molecular Cell Perspective argues translation is a core one, not just repression. Next: live high-res imaging + probing condensate dynamics should let us get at where translation starts and how condensates shape it.

The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes: 🖌️ Dozens more images with duplications or painting 🖨️ Hundreds of blots that all share the same background (behold slideshow below)

It was a pleasure to help @mariodelr.bsky.social, @henriqueslab.bsky.social and everyone on this new way to think and plan our microscopy experiments!

📚 Textbook rewrite: #Yeast reveals lysosomes get protons directly from mitochondria via membrane contacts, not from cytosol as thought. Aging breaks these contacts, causing de-acidification that disrupts autophagy & cellular function 🧬 #YeastResearch 🧪 #SGD 🔬 www.yeastgenome.org/reference/S1...

For 40 years the 8-nt ‘Kozak Sequence’ was thought to mark sites of translation initiation. In a new study, we revise this model by identifying an ~80-nt sequence—the extended Translation Initiation Sequence (eTIS)— that guides ribosomes to correct start sites. 🧵 www.biorxiv.org/content/10.6...

Happy Sunday! It has been another fantastic week for our beloved #oocytes! The streak on CPLs continues with this awesome work from Miguel Leung: pubmed.ncbi.nlm.nih.gov/41986725/! Full issue here: biomed.news/bims-cebooc/... Thanks @biomednews.bsky.social for preselecting.

Happy relaxing Sunday eggficionados! Enjoy the latest papers on oocyte cell biology here: biomed.news/bims-cebooc/... The CPLs are on fire these days, here new insights on their functions: pubmed.ncbi.nlm.nih.gov/41959504/ #cebooc is powered by @biomednews.bsky.social

💥🥚 ANR-funded Assistant Engineer position open in our “Control & Dynamics of the Ovarian Cycle” group at IBDM Marseille 🇫🇷 Work on mouse lines + lab logistics + follicle culture/imaging 🔬 🗓️ Apply by 26 Jan: emploi.cnrs.fr/Offres/CDD/U... Please share widely — especially with colleagues in France!

Periscelis wheeleri, the most grumpy old man fly I've ever seen

Postdoctoral position in the team of Alessandro Barducci in the CBS, focusing on multiscale simulations of RNA-driven condensates, with applications in synthetic biology. More information : www.cbs.cnrs.fr/images/jobs/... The position is expected to start in early 2026, with some flexibility.

Liquid-liquid phase separation of HP1? Not in our mouse fibroblasts… This paper by the Fabian Erdel group offers a fresh perspective on how this can be reconciled with seemingly conflicting reports: The phase separation propensity of HP1 decreases from yeast → fly → mouse. doi.org/10.1038/s414...

Is euchromatin really “open”? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.social‬ @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing. www.biorxiv.org/cgi/content/...

RNA droplets that grow and segregate by ribozyme catalysis. Check out our latest preprint www.biorxiv.org/content/10.1101/2025.08.29.673008v1

Happy to share our community note published in Nature Communications @natcomms.nature.com. In this 14-page article (34 authors from 9 countries), we discuss current practices in phase separation. We hope it will be useful to the biomolecular condensate community. www.nature.com/articles/s41...

What controls condensate composition and function beyond binding stoichiometry? We show that condensate microenvironments play a key role, and can be probed by a micropeptide killswitch in live cells. www.nature.com/articles/s41... in @nature.com ‬ with @yaotianzhang.bsky.social Denes Hnisz & team.

New method drop: HCR-Proxy, a modular proximity labelling approach to profile local proteome composition around RNA at nanoscale, subcompartmental resolution. Thereby we resolved nested nucleolar layers and uncovered the grammar of spatial protein partitioning. www.biorxiv.org/content/10.1...

We are very happy that XiaoYan's (Andy!) paper is out, congratulations to all authors @cellpress.bsky.social @xiaoyan52802927.bsky.social www.cell.com/cell/fulltex... Demixing of TDP-43 inside stress granules generates pathological aggregates linked to ALS/FTD @mpi-cbg.de @biotec-tud.bsky.social

#mRNADecay #Ribosome #Frameshift #Scerevisiae #NMD #CodonOptimality Ribosomes modulate transcriptome abundance via generalized frameshift and out-of-frame mRNA decay: Molecular Cell www.cell.com/molecular-ce...

1/ Super excited to share my first preprint from @andersshansen.bsky.social Lab! We used MINFLUX to track chromatin (H2B-Halo and Fbn2 locus) at an unprecedented 200 μs, then combined it with SPT to span μs-minutes (H2B) or SPT & Super-Res Live-Cell Imaging (SRLCI) to span μs-hours (Fbn2)

Very excited to have been a part of this great work with @matteomazzocca.bsky.social, Simon Grosse-Holz, @jessematthias.bsky.social, and @andersshansen.bsky.social. Please check it out now! www.biorxiv.org/content/10.1...

Forget liquid-liquid phase separation, here comes solid phase transition 😱 www.nature.com/articles/s41...

Hiro's paper is out! @hiro-cg5445.bsky.social Collaboration and Tour de Force with Richard Wheeler @zephyris-science.bsky.social and many other coauthors. Big congratulations and thank you to anyone involved! www.nature.com/articles/s41...

👏new paper👏 ! Our preprint is now accepted at MBoC @ascbiology.bsky.social Congrats to co-1st authors Junnan (new PI at OSU!) and Weiyi (now a Yale grad student) and 🙏 to collaborators Melissa & Jordan @myosincity.bsky.social Funded by NIH grants & NSF GRFP! www.biorxiv.org/content/10.1...

I am excited to share the latest paper from my lab where we leverage the selection bias of condensate-promoting oncofusions to uncover molecular rules governing condensate specificity and function. www.cell.com/cell/fulltex....

RNA xkcd.com/3056

Excited to announce the newest member of the flock - STARLING (conSTruction of intrinsicAlly disoRdered proteins ensembles efficientLy vIa multi-dimeNsional Generative models). www.biorxiv.org/content/10.1...

Not sure if anyone posted this on BSky, but here is a 'condensate' (pun intended) of the latest #RNA granules papers 'en vogue': www.cell.com/molecular-ce... #RNAsky #RNAbiology #MolCondensates

1/n Excited to share our new preprint! Using live imaging in Drosophila embryos, we show that RNA Pol II clusters switch from sites of initiation to elongation during zygotic genome activation and that they are stably associated with an active gene: www.biorxiv.org/content/10.1...

Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15) www.biorxiv.org/content/10.1...

📢 The Dormann Lab is looking for a PhD student interested in the molecular mechanisms of neurodegeneration and biomolecular condensation! Interested in one of our research projects on disease-linked RNA-binding proteins, such as TDP-43 and FUS? Apply to this fantastic PhD program by February 7 👇

I am thrilled to share the first manuscript from my group on identifying sequence and structural features promoting the condensation of RNAs that are organising highly interconnected RNA networks, thereby expanding our understanding of higher-order transcriptome assemblage. shorturl.at/pmLIj #RNASky

Protein codes promote selective subcellular compartmentalization | Science www.science.org/doi/10.1126/...

We are looking for a post-doc to join us in Oxford @biology.ox.ac.uk to study epigenetic inheritance! The project will use Cut&Run and RNA-seq to map and track new epimutations as they arise in Drosophila populations across multiple generations. Feel free to share! my.corehr.com/pls/uoxrecru...

Check out our most recent preprint where we describe Mecp2 as a régulatrice of cGAS-STING associated signaling. 🧪🔥

Our paper on Stopless-ORF Circular RNAs (socRNAs) is now out in Cell. By high-res tracking and comparing translation by either single or multiple ribosomes, we find that ribosomes cooperate to overcome pausing to ensure fast and efficient translation www.cell.com/cell/fulltex...

Phosphorylation of a nuclear condensate regulates cohesion and mRNA retention #RNAbiology #RNAlocalization www.nature.com/articles/s41...

1/ 🚨We’re thrilled to share our latest study: "Cellular Function of a Biomolecular Condensate Is Determined by Its Ultrastructure" 🌟 www.biorxiv.org/content/10.1... How do biomolecular condensates achieve their cellular roles? It comes down to their internal structure. 🧵⬇️

What a fantastic way to start the year! The KoiralaLab (unfortunately not here yet) have just developed RNA derived antibodies to visualize RNA in live cells! This seems to be a promising alternative to the MCP-MS2 system whilst offering potential multiplexing capabilities. #RNA, #RNASky, #imaging

It's celebration time: our first contribution to a better understanding of RNA structure and dynamics in biomolecular condensates together with Alberti lab - cool data from in vitro to in condenso to in vivo take a look 👇 @cmcb-tud.bsky.social @tudresden.bsky.social www.cell.com/molecular-ce...

Happy holidays~ ✨ Zebrafish embryo injected at 1-cell stage with mRNA expressing fluorescently-labeled Histone H2B. LUT: Grays, mpl-magma, Green Fire Blue. #ZebrafishZunday #DevBio #FishSky 🧪 youtu.be/RbouVtn9UR8

We are recruiting an enthusiastic engineer to identify isoform-splicing regulation in the developing Drosophila embryo with transcriptomics & genetics in our lively team in @igmm-montpel.bsky.social Please reshare! #jobOffer #RNA #hox #Drosophila #Nanopore #splicing emploi.cnrs.fr/Offres/CDD/U...

There are now 24 step-by-step guides, explaining how to create these ⬇️ data visualizations with R/ggplot2 - I hope this is a useful resource. Find them all here: joachimgoedhart.github.io/DataViz-prot...

Excited to share my new paper implicating the 3D genome in epigenetic inheritance. We show that chromatin contacts are able to trigger epimutation in Drosophila. I'm very thankful for the support of Giacomo Cavalli, @psarkies.bsky.social and to all others involved! www.cell.com/molecular-ce...

www.biorxiv.org/content/10.1... incredible study from Huabin Zhou and the Villa & Rosen labs -- a blueprint for mapping human chromatin with cryo-ET. The future of chromatin structural analysis in situ has arrived.

Check out my new post, where I discuss a couple of lesser-known functions of #tRNA during #translation and regulation of #gene expression. #RNA #RNAsky #RNAbiology rnastuff.com/2024/12/05/n...

Postdoc positions in epigenetics! We have several opportunities to investigate big picture questions on (epi)genome function & mechanisms Based @embl.org near Rome (Italy), we offer full scientific support/facilities, training & funding. Also...☀️🏛️🍕🏖️ See👇or contact me for other options. Pls RS