Welcome @shiori-iida.bsky.social , it’s great to have you in the lab! Excited about new directions in chromatin rheology and cell fate 🤓

I’ve recently joined @sarawickstrom.bsky.social lab at @mpi-muenster.bsky.social as a postdoc! I’ll keep doing exciting science with joy and passion 🔬💫 Huge thanks to @kazu-maeshima.bsky.social lab for all the support during my PhD journey⛰️ I’ll start by tasting every single sausage here🇩🇪

How do #stemcells integrate information to coordinate fate decisions? Delighted to finally see our work showing how growth factors regulate the mechano-osmotic state of the #nucleus and #chromatin to control #pluripotency exit out! www.nature.com/articles/s41... see 🧵 👇

Is euchromatin really “open”? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.social‬ @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing. www.biorxiv.org/cgi/content/...

Euchromatin is mostly condensed⁉️ With @shiori-iida.bsky.social , We revealed the detailed structure of euchromatin using super-resolution imaging. Cohesin regulates the function of euchromatin via chromatin dynamics! (1/n)

One more movie! Using the RL algorithm (from pnas.org/doi/10.1073/...), @masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility. Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)

Our new preprint is out! We combined single-nucleosome imaging and 3D-SIM to reveal: 🔹 Euchromatin forms condensed domains, not open fibers 🔹Cohesin loss increases nucleosome mobility without decompaction 🔹Cohesin prevents neighboring domain mixing Full story & movies👇

FINALLY! Challenging to publish but we believe it is an important discovery: rdcu.be/eATFz 💚 Thanks to the team ‪@biswashere.bsky.social‬, Omar Muñoz, ✨Q✨ C. Hoege, B. Lorton, R. Nikolay ‪@matthewkraushar.bsky.social‬ @dshechter.bsky.social @gucklab.bsky.social @vasilyzaburdaev.bsky.social‬ 💚

My #PhD defense work is on #Biorxiv! Link: biorxiv.org/content/10.110… Using single-molecul#imagingng, we showed that liquid-like BRD4-NUT condensates locally constrai#chromatinin vi#bromodomain-dependentnt crosslinking. I thank my supervisor, Prof. @kazu-maeshima.bsky.social, for his support!

Our new preprint 🧬🔗 www.biorxiv.org/content/10.1... BRD4-NUT forms liquid-like condensates that locally constrain nucleosomes via BRD4-mediated crosslinking— physical control of #chromatin by #LLPS transcription condensates. @semeigazin.bsky.social @katsuminami.bsky.social @masaashimazoe.bsky.social

📢 NIG Postdoc Fellowship Call! www.nig.ac.jp/nig/career-d... 🧬We’re seeking motivated postdocs to join our lab. 🔬We study chromatin behavior in living cells using advanced live-cell imaging and quantitative analysis: www.science.org/doi/10.1126/... doi.org/10.2183/pjab... Interested? Please apply!

Check out this exciting preprint from @aznarbenitahlab.bsky.social discovering a new mode of crosstalk between YAP and the circadian regulator BMAL1. Congrats @juliabonjoch.bsky.social and @guiomarsolanas.bsky.social for fantastic work and thank you for the fun collaboration!

In developing embryos, cells move a lot! Plenty of that movement is random. Is random cell mixing a feature or bug for tissue patterning? Turns out, it’s both! Excited to share the 1st preprint from my postdoc w/ Sean Megason @seanemcgeary.bsky.social and Allon Klein. 1/20 doi.org/10.1101/2025...

🎥 Video: See chromatin in action — each dot is a single nucleosome, dynamically fluctuating in a living HeLa cell (TMR-labeled, 50 ms/frame). 🔬 Original paper: www.science.org/doi/full/10....

🧬My retrospective chromatin review is out @Proc Jpn Acad: doi.org/10.2183/pjab... The textbook view of #chromatin as 30-nm fibers is fading. A new paradigm: chromatin forms #liquid-like domains—locally dynamic, yet globally stable at the chromosome level (viscoelastic)—supporting genome function. ✨

Our new paper is out@ScienceAdvances👇 www.science.org/doi/10.1126/... 🧬Our Repli-Histo labeling marks nucleosomes in euchromatin and heterochromatin in live human cells. 🔍 @katsuminami.bsky.social et al. have developed a chromatin behavior atlas within the nucleus. 1/2

On top of that, I’m honored to receive the SOKENDAI Award from my university for my PhD work! I can't thank @kazu-maeshima.bsky.social, my labmates, and all the NIG members enough for their support. This recognition gives me even more motivation for the journey ahead!

It took blood, sweat, tears, too much coffee☕️, and just the right amount of alcohol🍺—but I finally got my PhD! Huge thanks to everyone supported me, especially my mentor @kazu-maeshima.bsky.social, labmates, friends, and family. I’m excited to keep chasing big questions and growing as a scientist!

Our new preprint is out＠bioRxiv: www.biorxiv.org/content/10.1... @masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

Our new review on how the #chromatin domain is formed in the cell is now available @Curr Opin Struct Biol.📄✨ We critically discuss the domain formation mechanism from a physical perspective, including #phase-separation and #condensation. 📥 Free-download link: authors.elsevier.com/a/1keGn,LqAr...

Today in Science Advances: "Mature chromatin packing domains persist after RAD21 depletion in 3D". Researchers at CPGE integrated measurements of genome connectivity and physical structure to deepen our understanding of chromatin organization #chromatin #genomics www.science.org/doi/10.1126/...

Thanks🎅🏼🦌 Merry Christmas all. @shiori-iida.bsky.social @dai.tsujino #NIGchrismtmass

We are seeking a motivated postdoc to join our lab!  Our research focuses on live-cell imaging/analysis to reveal how chromatin behaves in living cells and how its behavior contributes to cellular functions. www.nature.com/articles/s41... www.science.org/doi/10.1126/... Interested? Please DM me!