Publication alert: Our paper on domain insertion predictions in proteins is now out in @natmethods.nature.com in its final form: rdcu.be/ey7w3 Also check out the nice perspective by @noahholzleitner.bsky.social and @grunewald.bsky.social : www.nature.com/articles/s41...

Our work on rationally engineering allosteric protein switches is now out in Nature Methods: www.nature.com/articles/s41... Thanks a lot to @grunewald.bsky.social and @noahholzleitner.bsky.social for the comprehensive news and views: www.nature.com/articles/s41...

Dual SORT LNPs for multi-organ base editing go.nature.com/3HC9EHt

For the first time, doctors have created a customized treatment using the revolutionary gene-editing technique known as CRISPR to treat a baby with a rare, life-threatening genetic disorder.

Today "a milestone in the evolution of personalized therapies for rare & ultra-rare inborn errors of metabolism" —the 1st human to undergo custom genome editing —from decades of NIH funded research www.nejm.org/doi/full/10.... @nejm.org www.nejm.org/doi/full/10.... www.nytimes.com/2025/05/15/h...

Evolution-guided protein design of IscB for persistent epigenome editing in vivo - @zhangf.bsky.social @broadinstitute.org @hhmi.org go.nature.com/44XVBph

By combining high-throughput protein engineering with machine learning, bespoke SpCas9 enzymes are identified that outperform evolution-based and engineered nucleases and base editors in human cells. www.nature.com/articles/s41... #NBTHighlight

AlphaFold is amazing but gives you static structures 🧊 In a fantastic teamwork, @mcagiada.bsky.social and @emilthomasen.bsky.social developed AF2χ to generate conformational ensembles representing side-chain dynamics using AF2 💃 Code: github.com/KULL-Centre/... Colab: github.com/matteo-cagia...

We are super excited to share our work on #InnateImmune sensing of #AAV 🦠🧬 in the 🧠, just out in @natcomms.nature.com ! 🤩 Huge congratulations to all the team and collaborators 💪🏻 And our funders 💰 @erc.europa.eu #FondazioneTelethon and #FNIH 🙏🏻 www.nature.com/articles/s41...

Our latest work now online in Cell: Rewriting regulatory DNA to dissect and reprogram gene expression Our new method (Variant-EFFECTS) uses high-throughput prime editing + flow sorting + sequencing to precisely measure effects of noncoding variants on gene expression Thread 👇

RFDiffusion2: a diffusion model that scaffolds enzyme active sites at the atomic level enables the generation of functional de novo enzymes www.biorxiv.org/content/10.1...

www.cell.com/cell/fulltex... ENVLPE, our system for VLP-based delivery of gene editors, is now published in @cellcellpress.bsky.social. We showed efficient delivery of base- and prime-editors in primary T cells & in vivo retinal models. All core constructs are available from @addgene.bsky.social.

Dive into our lab’s first peer-reviewed publication—co-led with our friends @benadler.bsky.social and Muntathar Al-Shimary from the @doudna lab! If you’ve already read the preprint, don’t miss the new updates: doi.org/10.1038/s415... @naturemicrobiol.bsky.social

Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15) www.biorxiv.org/content/10.1...

Twitter: LNP/mRNA therapeutics and AAV-based approaches require cell-level precision to achieve success without significant toxicity to other cells. To enable this, we developed SCP-Nano: an AI and tissue-clearing-based technology for precision nanotechnology. www.nature.com/articles/s41...

New paper from our lab (@erturklab.bsky.social) We show how tissue clearing, light sheet microscopy, and AI-based image analysis can be combined to determine the biodistribution and drug delivery efficiency of nanocarriers throughout entire mouse bodies with single-cell resolution.

🧪1/ 🚨New paper on ecDNAs from our lab! We reveal a strategy to engineer extrachromosomal DNA (ecDNA) amplifications in cells & mice. Let's dive in! Let’s dive in! 🧵👇 www.nature.com/articles/s41...

I could not be more thrilled to announce the Nature Methods @naturemethods.bsky.social Method of the Year is Spatial Proteomics! Please see our editorial as a roadmap to the fantastic content in this special issue! www.nature.com/articles/s41...

Imagine seeing a disease's impact on every cell in the body. Now, you can! We introduce MouseMapper, an AI tool to reveal system-wide disease perturbations. It reveals obesity-induced changes in facial nerves & body-wide inflammation 👉🧵https://biorxiv.org/content/10.1101/2024.08.18.608300v1.full.pdf

Our new preprint is out - please share! We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals. Grab a ☕ and let’s dive in. 🧵🧪 1/n #CRISPR #geneediting

By definition, enhancers can activate from a distance. But with increased distance between enhancer and promoter, the activation drops. To study this systematically, we build a synthetic locus: www.cell.com/molecular-ce... 1/12

2X #DilatedCardiomyopathy GWAS MATG #NatGenet 2024 reveal novel genes/cellular players PROX1 - #LymphaticEndothelialCell ERG - #EndothelialCell DGKG - #SmoothMuscleCell MAPT/Tau validated in both studies >9k case www.nature.com/articles/s41... >14k case www.nature.com/articles/s41...

Online @cellpress.bsky.social, we reveal a protein shell that helps diatoms fix 20% of global #CO2 🧪🌊🌾🧶🧬🌍 Shimakawa et al: www.cell.com/cell/fulltex... Nam et al: www.cell.com/cell/fulltex... @biozentrum.bsky.social press release: www.biozentrum.unibas.ch/news/detail/... Read on for more... 1/🧵

Interesting concept. Recruiting Cas9 to nascent transcripts allows coupling of editing efficiency to expression of the target gene. www.nature.com/articles/s41... #CRISPR

This is really interesting. It's probably a great way to deal with resistance mutations in the future.

New paper from our lab @erturklab.bsky.social www.cell.com/cell-host-mi...

Very excited about the first preprint from our lab at TUM! This is a fun gene & cell therapy collaboration led by @schmidts.bsky.social's lab. We use generative #AI to design new #CARTcell therapies. Check out Andrea's thread & thanks to the whole team! www.biorxiv.org/content/10.1...

Cancer escapes immunotherapies by mutating its surface, meaning even groundbreaking #CARTCell therapies fail too often. But what if #GenerativeAI could design CAR-T with de novo binders faster, targeting resistant tumors? 🧵 www.biorxiv.org/content/10.1... (1)

On this day 75 years ago, Linus Pauling published his classic report on abnormally charged hemoglobin in #sicklecelldisease in Science, hence the first “molecular disease”. 🧵 www.science.org/doi/10.1126/...

The typeset version of our article, "Reconstruction of the human amylase locus reveals ancient duplications seeding modern-day variation", is now online. This thread will provide an overview of how gene duplications have shaped modern variation in the amylase locus. www.science.org/doi/10.1126/...

A few more letters... and we can finally write amino acid sequence with protein structure encoded by amino acid sequence? 🧬🤔😀 @chrisfrank662.bsky.social , Dominik Schiwietz, Lara Fuss, Hendrik Dietz www.biorxiv.org/content/10.1...

Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys go.nature.com/3CGO1Dy

@grunewald.bsky.social told me about this amazing tool yesterday: www.biorxiv.org/content/10.1... Seems worth checking out!

Quite a few new entries on the CRISPR list incl. @nevillesanjana.bsky.social @grunewald.bsky.social @sdomcke.bsky.social @rosswilsonlab.bsky.social @leopoldparts.bsky.social @bkleinstiver.bsky.social @mgdurrant.bsky.social go.bsky.app/DEwe6ZD

We strongly suggest that academic publishers and other platforms that host research rapidly implement a Share to Bluesky button for their articles. Here's how: docs.bsky.app/docs/advance... #AcademicSky #HigherEd #Altmetrics

New here and excited to share our new paper on transcriptional effectors! Interested to discuss context-specificity of gene regulation? Or how to get CRISPRi/a working in new contexts? 👋 www.nature.com/articles/s41...

Totally missed this when it came out. Very cool!

A latest product launch from NVIDIA digital bio has dramatically improved protein structure prediction by integrating GPU acceleration to multiple sequence alignment. developer.nvidia.com/blog/boost-a...