this is extremely cool: www.nature.com/articles/s41...

Whole-body Single-cell Spatial transcriptomic atlas of adult Danionella ~1 cm long scRNAseq MERFISH (500-gene) HCR Endothelial cell podxl+ (Fig S8) Vascular vs Visceral mural cell (Fig S10) Peter Reddien lab bioRxiv 2026 www.biorxiv.org/content/10.6...

#MetaplasticBreastCancer n=44🇬🇷 YAP/TAZ immunoreactivity in MpBC Not corr. CTGF CCND1 Not corr. Histological/Molecular subtype Not corr. Disease-free survival Marginal corr. Overall survival Chemo➡️⏫survival Recurrence➡️⏬ survival HR>100😨 #CurrIssuesMolBiol 2025 www.mdpi.com/1467-3045/47...

VERY excited that @harmitmalik.bsky.social will be giving the UNC Biology seminar tomorrow, and is featured in one of Bob Goldstein's gig posters. I missed him the last time he cams through (I was away giving a talk) and thus have never heard him speak

New preprint alert! We developed an automated framework to optimize tracking pipelines for bacteria, along with a nice integration of tools for bacteria imaging, in the recently released TrackMate 8 (Fiji plugin). www.biorxiv.org/content/10.6... 👇🧵

A menagerie of COS cells for #MicroscopyMonday 🧪

Microcephaly-lymphedema-chorioretinopathy syndrome (MLC) is an autosomal dominant condition. This issue’s cover: Ogmen et al. report on clinical features and disease mechanisms in MLC involving KIF11 pathogenic variants. doi.org/10.1172/jci....

#Postdocs! Going on academic job market? Apply for SDB GetHIRED! Take stock of what you want, know your options, prepare an outstanding job packet, organize your research & chalk talks, perfect your interview. < 1 month till Deadline: March 5 @socdevbio.bsky.social www.sdbonline.org/gethired

EVBO Paper of the Month! Congratulations to the authors on this fantastic contribution! Transcriptomics- and 3D imaging–based characterization of the lymphatic vasculature in human skin by Aline Bauer and colleagues. rupress.org/jem/article/... More information: evbo.org/index.php/pa...

Job alert: eur04.safelinks.protection.outlook.com?url=https%3A...

Hao's Preprint Reading List February 9 2026 A nematic framework for sprouting angiogenesis Cell compliance & tissue rupture Cancer drives atherosclerotic plaque vulnerability Sheep model of PAH Whole-body single-cell spatial atlas of Danionella🐟 docs.google.com/document/d/e...

🤝 ICYMI: ELRIG x Zeeks - Art for Geeks | Media Partnership 2.0 We’re delighted to continue our partnership with ELRIG as media partners for Cell & Gene Therapy 2026, taking place in Cambridge. 👉 Register here: elrig.org/portfolio/ce... #ELRIG #SciComm #Conference #MarCom

So grateful to @epimechfc.bsky.social for citing our work! It’s a classic from the lab, forever thanks to @colomlab.bsky.social

Post-stretch (3 d) response of 👤fetal Airway Smooth Muscle Cell Mechanical Stretch mimicking continuous pos airway pressure▶️cyclic 5% 0.67 Hz + static 5%/10% vs control▶️cyclic 5% 0.67 Hz ⏫Proliferation ⏫IL-6 ↔️Collagen/Fn deposits Piezo-indie #AJPLung 2025 journals.physiology.org/doi/full/10....

Latest preprint from the lab - what does S1P do to neutrophils ? Click the link to find out… www.biorxiv.org/content/10.6...

I just started a cell biology and biophysics group at EPFL, Switzerland that will investigate how cells endure mechanical stress and release large cytoplasts. Get in touch if you’d like to join! See more at celldynamicslab.com

📄 Finally, the success of the probe comes in part from its availability. The University of Geneva licensed the probe to @spirochrome.com @lreymond.bsky.social which made it available around the world, along with new versions like Halo-Flippers 🔗 bsky.app/profile/epim...

📄 A major effort is carried out by the community to benchmark Flipper-TR alongside other membrane probes. This elegant paper from @sciezgin.bsky.social quantified the FLIM response of several probes in parallel 🔗 bsky.app/profile/epim...

📄 Methods: A practical FLIM analysis-focused guide: acquisition, fitting strategies, phasors, and interpretation. It clearly explains how lifetime reflects lipid packing, and where over-interpretation of “tension” can go wrong. 🔗 bsky.app/profile/epim...

📄 Tension gradients during migration. Using Flipper-TR FLIM, we showed that membrane tension gradients exist in all adherent cells and are actively maintained by actin and adhesion, not passively equilibrating. @rouxlab.bsky.social and Aumeier lab 🔗 bsky.app/profile/epim...

📄 In cells, osmotic shocks shift Flipper-TR lifetime consistently with increased or decreased membrane tension, provided composition is controlled. This paper also compared quantitatively tether force and F-TR lifetime measurements @chloeroffay.bsky.social 🔗 bsky.app/profile/epim...

💡 By tuning lipid composition and applied tension in GUVs, Flipper-TR lifetime could be quantitatively related to membrane mechanical state, establishing the physical basis for cellular measurements made later.

How does Flipper-TR work? Flipper-TR inserts in the lipid bilayer. Its mechanosensitive core rotates, sensing the lipid packing. The electron-rich head group confers the plasma membrane specificity... but other versions exist for alternative targeting! 🔗 bsky.app/profile/epim...

First, why Flipper-TR? It comes from swimming fins, also called swimming flippers, because of their shape! coined by the first author of the original paper, Marta Dal Molin, at @matilegroup.bsky.social. TR stands for ‘tension reporter’. Here’s a bit of history: www.unige.ch/campus/numer...

Fibroblast TGFβ signaling in systolic & diastolic🫀functions Protective▶️Lean 🐭 Pathogenic▶️Diabetic db/db🐭 Evidence from Fibroblast Tgfbr2 or Smad3 KO🐭 Speckle-tracking echo 👉Peak systolic/diastolic longitudinal endocardial/epicardial strain #CardiovascRes 2024 academic.oup.com/cardiovascre...

Hello epithelial mechanics fans!! I’m Juanma @juanmagararc.bsky.social 👋 I work on cell mechanics (see celldynamicslab.com) and use Flipper-TR FLIM to probe membrane biophysics in cells. Join me on this tour about Flipper: what it measures, strengths, advice, cool case studies, and cute drawings!

For both #RareDisease month and #MarfanSyndrome awareness month, I thought I'd share this article I wrote for the @ahajournals.bsky.social about being a patient-scientist working on developing genetic therapies for my condition and related conditions.

In spatial omic, using standard 2D sections undersamples 3D tumors and tissues, always. We asked how many slides or cores of a TMA are needed to capture the variations in tissue composition in a single 3D tumor? A lot! Download here: www.sciencedirect.com/science/arti...

FGF9:c.382-1264C>T a intronic SNP with Hu Sheep🐑 body weight (1070 male, 80~180 days of age) PIC 0.35 Heterozygosity 0.45 HWE p=0.89 Located near open chromatin region & H3K27ac+ locus Act in pituitary gland? How does it affect FGF9 level? #Animals 2025 www.mdpi.com/2076-2615/15...

'CD8+ T cells from patients with homozygous familial hypercholesterolemia, who harbor inactivating LDLR mutations, exhibited reduced proliferation and ICAM-1 expression upon activation.' #Immunology #Metabolism www.cell.com/iscience/ful...

I am looking forward to visiting the Dept of Chemical Engineering at the University of Washington. My seminar on CODA and new CAR T cells for solid tumors will be this Tuesday, 4 – 5 p.m in the PAA www.cheme.washington.edu/calendar?tru...

Dynamic actin waves in a zebrafish embryo. Credit to @aaandmoore.bsky.social & Dvir Gur. #ZebrafishZunday 🧪

An intracellular meteor shower. EB3 comets tracking growing microtubule plus-ends in a cultured cell.

Not something you see in textbooks very often: tripolar mitosis.

Generation of publication-ready(!😈🧐) statistical plots + schematics using AI 🎃 PaperBanada🍌 vs NanoBanada-Pro dwzhu-pku.github.io/PaperBanana/ arxiv.org/abs/2601.23265

The beauty of the kidney arterial tree for this #FluorescenceFriday. Evans Blue dye labels the vasculature. Image courtesy of postdoc Sarah McLarnon.

Together we think these mechanisms contribute to traffic going only in the forward direction, and that it keeps COPII carriers and Golgi precursors from fusing with the wrong organelles, which would mix everything up rather than keeping the different compartments separate. More to come! /19

In pathbreaking studies published 25 years ago, Rothman's lab put purified SNAREs in synthetic membrane vesicles, and reported that only one arrangement works: Qc on one side and R, Qa, and Qb on the other. They published two papers in Nature, and one in PNAS making this argument. 9/

This turns out to be a lot harder than the above cartoon makes it look. In a cell, dozens of proteins are needed and they have a complicated choreography that's only partially understood. In 1998 Schekman's lab at UC Berkeley was able to reconstitute formation of a COPII carrier (PMID 9568718). /6

We're reductionist biochemists. We want to take just this little bit of the pathway, separate all the pieces, like Legos, rebuild the functioning transport event in a test tube: disassembly of the COPII protein coat, and then tethering, docking, and fusion of the membrane carrier at the Golgi. /5

There is also an endolysosomal pathway that takes things into the cell from outside, for degradation. The pathways intersect. It gets very complicated. This nice diagram is from my friends Ben Glick and @juanbonifacino.bsky.social. Even it is hugely over-simplified. 4/

As Fran was nice enough to highlight this, I thought I'd put up a brief thread. 3 in 10 proteins that our cells make are either embedded in membranes (ion channels, adhesion molecules, etc.) or secreted (insulin, antibodies). They move through the secretory pathway. 1/

Advances in genetics, signaling, & modeling of venous malformations Research History Genetic & Molecular Drivers - TIE2-L914F PIK3CA-H1047R Experimental Models - hiPSC, Vessel-on-a-chip & Knock-in🐭 Lovely & timely reading😁 @boscololab.bsky.social #FrontCVM 2026 www.frontiersin.org/journals/car...

In honor of our "HYP Team" gearing up for a big data generation sprint, also giving human 🧠 some 💗 for #FluorescenceFriday. So cool to see these lovely oxytocin neurons 🔴 in the paraventricular nucleus of hypothalamus! Image credit to @svitlanabach.bsky.social @yufengdu.bsky.social 🔬🧪

Excited to share our new paper and lipid- and protein-directed photocatalytic labeling method (POCA) just out in @natchembio.nature.com. tinyurl.com/2kcxuvvv. Big congrats to first author Andrew Becker and the whole team for launching our lab into the wild world of singlet oxygen interactomics.

I thought NSF PRFB is back but it turned out it's NSF AI PRFB is here. Regardless, if you're interested in evo-devo in flowers, definitely reach out - we will put some AI in to get real work done! #PRFB #postdoc

this is very interesting

🧵 [1/6] Unlocking bacteria’s full biotech potential! Discover strategies to boost recombinant protein secretion: 🔗 inpst.net/advancing-re... 🌐 #Biotechnology #CRBIOTECH @shawneexo.bsky.social @haoyin.bsky.social @mquigley1963.bsky.social @oleksandrkashuba.bsky.social @hipecsurvivor.bsky.social

Actually, it's only 4 replicates per group (still not bad): "Xenium spatial transcriptomics experiments were performed on aortas from db/db and db/dbDAPA groups (n=4/group)." Unfortunately the GEO accessions are still private...