Merry Fishmas, shoalmates! We hope you have a lovely festive season 🎄🐟 🧪

My continued biological education Protein neddylation & its role in health & diseases Cullin & non-cullin substrate (cGAS, PTEN, P53, Huntingtin, Histones & Ribosome...) Tissue-specific E1 NAE1 KO▶️🫀🧠development Endothelial E3 Sag KO▶️defect Vasculogenesis #STTT 2024 www.nature.com/articles/s41...

Why do babies kick in the womb? Today I'm delighted to share our latest paper, led with great creativity by postdoc @cpane94.bsky.social, in wonderful collaboration with Niamh Nowlan at UCD Dublin. "Maternal exercise rescues fetal akinesia-impaired joint and bone development" A paper thread:

After moving to LSU Health Shreveport for two months, nothing can be more exciting than getting this spatial data to get started💪

My lab is hiring! Several exciting projects are waiting for you! jobs.sciencecareers.org/job/674732/p...

Happy Holidays everyone! 🎄🎁🥂

Noncanonical Sirtuins FAM118B + FAM118A undergo filamentation & possess NADase activity 🤠 They are enriched in a special subnuclear domain & deplete cellular NAD+ A role in evolutionarily conserved innate immunity, like bacterial antiphage sirtuins #NatureSMB 2025 www.nature.com/articles/s41...

#DBfeature Wish our authors and readers a Happy Holiday & a fruitful 2026!🥳🥳🥳

"Christmas is not in tinsel and lights and outward show. The secret lies in an inner glow. It's lighting a fire inside the heart. [...] It's glorious dream in the soul of man." ~ W. A. Peterson in 'The Art of Living' #MerryChristmas everybody✨🎄🎅 ➡️ eso.org/public/image... 🔭 🧪 #Universe

If you want to map the spatial composition of a tissue or organ, 3D mapping is not an option. It is absolutely necessary. Due to its inherent undersampling, 2D omic mapping is almost always misleading. See details here: www.sciencedirect.com/science/arti...

Merry Christmas

🎄 Wishing you and your loved ones a merry Christmas! 2025 was packed with exciting opportunities, such as 🔹ELRIG Drug Discovery 2025 Media Partnership 🔹Having our Keep it Science podcast on BBC Radio featured (again!) 🔹Closing our first customer in Saudi Arabia #SME #smallBusiness

👤Brain Organoids post-graft in 🐭prefrontal cortex & hippocampus They gain regional traits😆 Prefrontal cortex niche (vs hippocampus) ➡️⏫TH+ dopaminergic & VACHT+ cholinergic features ➡️⏫Astrocyte branching & interactions with neurites #AdvSci 2024 advanced.onlinelibrary.wiley.com/doi/10.1002/...

since you made it all the way to then end - we've already made transgenic lines with the mSG from Ando et al - it's amazing! here's a movie from our Cell Observatory project at the Advanced BioImaging Center 7/7

Felt a little festive at the microscope this morning for #FluorescenceFriday 🎄 Here’s the nervous system of a juvenile sea star ⭐️ Green = acetylated tubulin, red = nuclei Happy holidays!

#HutchinsonGilfordProgeriaSyndrome Intriguing 🐭data showing that Progerin-induced #ERStress #UnfoledProteinResponse in Aorta is NOT EC-origin Evidence from 1⃣Cdh5-tTA:tetop-LA G608G 🐭 2⃣Lmna G609G knockin 🐭 #LSA 2025 www.life-science-alliance.org/content/9/2/...

This is exactly why people claiming "it came up with a new set of experiments/ideas/concepts" are carrying water for the weasels trying to put genAI on everything. This is how we get science defunded. This is how your colleagues lose their jobs 🧪

🎄 Day 23 of the 24 Days of Development ✨🐣 Targeted protein degradation 🎯 allows precise removal of specific proteins 🧪🔬, letting researchers test their roles in real-time during development 👀🎉 doi.org/10.1016/j.yd...

Enjoy these human epithelial cell nuclei - especially the bagel with a hole right through the middle! MCF10A cells with labelled with DNA (blue; Hoechst), anti-lamin A/C (green), and F-actin (magenta; phalloidin). 3D z-stack taken with a Zeiss CellDiscoverer7 + LSM 900 Airyscan-2, 20X (NA 0.95).

YUP 👀🔥😐

Hao's Preprint Reading List December 23rd 2025 Ciliogenesis in Colitis Heart Organoids models Mitochondrial disease Neuroimmune modulation of Atherosclerosis Single-cell atlas of Breast Cancer >100 patients Subcellular Spatial Omics VEGFR2/3 & Lymphangiogenesis docs.google.com/document/d/e...

Blocking Integrin αV (Cilengitide or SB273005) aggravates ascending aortic aneurysm & dissection in 🐭+BAPN Transcriptional role of STAT1 on SMC contractile proteins Integrins αV αL ⬇️in👤type A #AorticDissection Plasma #Proteomics 11 case + 7 ctrl #JTCVS 2025 www.sciencedirect.com/science/arti...

🎄 Day 22 of the 24 Days of Development ✨🐣 Potassium channel Kcnj2 shapes electrical activity in embryonic mouse palate cells 🐭⚡ tuning gene networks that guide palate development and helping explain how disruptions can lead to cleft palate 🎁 doi.org/10.1016/j.yd...

This is cool stuff - imaging covalent drug biodistribution using tissue clearing methods

On the first day of the Christmas holidays, I got... a new preprint out! Challenging the reproducibility of our manual presynaptic actin nanostructure identification: www.biorxiv.org/content/10.6... (data from Bingham et al. @jcb.org 2023 doi.org/10.1083/jcb....) with @flevet.bsky.social

SenCID a 6-module single-cell transcript feature for #Senescence CROP-seq Smooth muscle cell ➡️SID2 Endothelial cell ➡️SID4 Fibroblast ➡️SID5 Capture 👤Lung aging (AT2), Skin aging (fibroblast), Osteoarthritis (chondrocyte), Atherosclerosis (aortic EC) #CellMetab 2024 www.cell.com/cell-metabol...

How does the axonal sub-membrane periodic cytoskeleton develop? Nick & Rohan from our lab found that building blocks are delivered in packets and co-assemble locally with actin for final structure. Fun collaboration with imaging Guru @christlet.bsky.social and his team. www.cell.com/iscience/ful...

We point to a possible role of spectrin droplets/condensates in allowing transport down the axon before actin triggers transition of patches into a stable submembrane scaffold. Great work from people in the team including @wiesner-t.bsky.social, @nicolasjullien.bsky.social and Christopher 🙏

On my last day in the lab for 2025... Our work with @roylab-ucsd.bsky.social on the formation of the membrane-associated periodic skeleton during axon development via droplet formation and condensation is out in @cp-iscience.bsky.social! www.cell.com/iscience/ful...

✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

Just stumbled on Mole, a MacOS command-line software that's great for some laptop end-of-the-year cleaning! Free an open source: github.com/tw93/Mole

Aligning different spatial omic data (histology, spatial proteomics, spatial transcriptomics, etc) is challenging. PhD students André Forjaz and Valentina Matos Romero developed PIVOT to do this really well and rapidly. Read about PIVOT here: www.biorxiv.org/content/10.1...

Amazing - Human Aortas can be cryopreserved at -80 °C with 10% DMSO for 12 months with histological (elastin, SMC, EC) & mechanical (subfailure stress, max stress, low/high stretch moduli) integrity largely retained #AortaEd #JTissEng 2025 journals.sagepub.com/doi/10.1177/...

🎄 Day 21 of the 24 Days of Development ✨🐣  Researchers reveal how oocyte growth in C. elegans relies on cytoplasmic bridges 🌉 that shuttle material from nurse cells to developing eggs 🥚❄️ doi.org/10.1016/j.yd...

MechanoEnhancer Fibroblast Matrix Stiffness 50 vs 1 kPa 👉Bulk RNAseq 👉On-plate Omni ATACseq (NO detachment required🤠) ▶️Anoikis mechanoenhancer (BMF) 👉HiCAR 👉CRSIPRi epigenomic editing 21.8% putative mechanoenhancers "skipped 5 or more genes"😎 #Science 2025 www.science.org/doi/10.1126/...

Bulk transcriptome + DNA methylome of 👤Ascending Aortic Dissection 5 TAAD vs 5 donor aorta Support Vector Machine-recursive feature elimination▶️ Key methylated genes ZC3H12A IRAK2 CCL5 Other genes TLR9 SERPINE1 IFNA1 HGF JAK3 IL11 NOD1 CXCL6 NAMPT HK2 #SciRep 2025 www.nature.com/articles/s41...

Human monocytes placed on a soft matrix undergo phase separation into micro domains. On glass/plastic, monocytes do not form such domains. See more details in our recent paper in Nature Materials: www.nature.com/articles/s41...

Just in time for the Holidays, our latest paper on how #amyloidbeta regulates the spatiotemporal properties of vascular L-type channels - implications for vascular function. Great work by Jade and team! @ucdavis.bsky.social, @ucdavishealth.bsky.social physoc.onlinelibrary.wiley.com/doi/10.1113/...

Please check out our job post @biologists.bsky.social! We are recruiting a Postdoc Scientist to develop cutting-edge tools and techniques for bioengineering human lymphatic networks, as part of an interdisciplinary collaboration with the group of Prof Dame Molly Stevens! bit.ly/3La9idq

Amidated gut hormone peptidergic signaling as a determinant of sex-specific metabolic and behavioral states in Drosophila @MolMetab www.sciencedirect.com/science/arti...

I would like to thank the great Rita Strack for creating this unique opportunity to write these two review papers on cell migration. ‪@ritastrack.bsky.social‬

UNC leaders just announced the closure of its world-renowned Area Studies Centers, claiming the $4 million they cost is unaffordable, when we paid Belichick $10 million to lose most football games.. Join me in signing this petition supporting a reversal of this tragic decision. c.org/Jgs5P4Lj94

I have an announcement to make. It's huuuge.....

Thank @kurianlab.bsky.social for alerting me to this amazing lymphovascular preprint! I only want to see a bit more evidence to capture in situ lymphatic cell death overlapped with lipid peroxidation!😆

Lymphatic Endothelium Preprint🚨 A comprehensive comparative analysis of Ferroptosis sensitivity b/w Lymphatic vs Blood Endothelial Cell Ferroptotic machinery controls 🐭Dorsal lymphatic growth E9.5-15.5 & Corneal lymphatic regression P16-22 bioRxiv 2025 www.biorxiv.org/content/10.1...

See Shino’s thread for details. For a high-level overview, here’s the press release: www.newswise.com/articles/imp...

We did a deep dive into aortic stenosis genetics, and took first steps to understand the genetics behind normal human variation in flow across the aortic valve.

Now that you have gathered you raw data from a cell migration assay, how do you analyze that data? In our review, we describe methods - including AI methods - to track migrating cells and extract as much information as possible. Read our paper here: www.nature.com/articles/s41...

#FGF9 or FGF2/4/8 ⏫UCP1 in both Brown & White Preadipocytes FGF-induced UCP1 can be artificially activated by TTNPB But NOT enhance browning or adipocytic differentiation per se Non-conventional anti-inflamm effect of UCP1 in preadipocyte #FASEBJ 2025 faseb.onlinelibrary.wiley.com/doi/10.1096/...

This study develops human pluripotent stem cell derived 3D neural organoid models that recapitulate sympathetic ganglion development and function, enabling the study of peripheral nervous system maturation, target organ innervation, and related diseases. www.cell.com/cell-stem-ce...