HexAufoil grids from Chris Russo's group @mrclmb.bsky.social gave amazing results for this 86 kDa complex!

New Preprint alert! Excited to share our latest work on the #MAPKinases from stellar PhD student Jill von Velsen. First structures of the MAP2K MEK1 activating its substrate MAPK between 2.9 and 3.6A - amazing resolutions for such a small and mobile complex! www.biorxiv.org/content/10.6...

Fully genetically encoded low-molecular-weight protein tags with defined shapes for direct molecular identification by cryo-electron tomography www.biorxiv.org/content/10.64898/2026.01.16.700029v1 #cryoem

New Title Alert: REPIC- REliable PIcking by Consensus (REPIC) is a consensus methodology for harnessing multiple cryogenic-electron microscopy (cryo-EM) particle picking algorithms. Learn more here: buff.ly/cCqyNHx #SBGrid #SBGridSoftware #StructuralBiology

Join us for a Presidential Lecture on 1/28 with Eva Nogales: "Visualizing Structure, Dynamics and Interactions Using Cryo-EM." Register: https://www.simonsfoundation.org/event/visualizing-structure-dynamics-and-interactions-using-cryo-em/ #science #biology

Charles Barchet et al.: Explicit correction of severely non-uniform distributions of cryo-EM views ##CryoEM##3DReconstruction##ViewFrequency... ##IUCrhttps://journals.iucr.org/paper?S2059798326000306

We are running the cryo-ET image processing workshop again this year! Come learn everything tomo: STA, segmentation, heterogeneity analysis from @baradlab.com @williamnwan.bsky.social and others! Apply!

Curious about cryo-electron tomography but not sure how it fits into your research? This ASCB webinar with Danielle Grotjahn, Ph.D., breaks down what cryo-ET can do, where it shines, and how it works alongside other imaging methods.

Excited to see this work on the cover of #MolecularCell. A massive, open #cryoEM #cryoET dataset capturing molecular organization inside intact cells, pushing what’s possible for #visualproteomics, method development, and computational reuse. Congratulations to the authors. ter.li/of4cgh

Our January software webinar will feature Sliz Lab postdoc Alice-Roza Eruera on Building CryoET Training Workflows Using the SBGrid Ecosystem. Be sure to join us on January 13th! Register here: buff.ly/wwNF6Zh #SBGrid #StructuralBiology #Webinars

Very pleased to be on the organizing committee of the EMBO course Structural Characterization of Macromolecular Complexes in 2026! We have a fantastic line up of speakers and tutors, please share widely! 👉apply here: www.embl.org/about/info/c...

Atomic resolution cryo-EM at 200 keV www.biorxiv.org/content/10.64898/2026.01.09.698548v1 #cryoem

Happy new year #teamtomo! We've resumed our OHSU tomo journal club, and this year I am going to be sharing it on bluesky. This week we did a roundtable, where attendees each brought a paper and did a 3-5 minute discussion of what they thought was cool about it. Here are the papers they discussed!

Fragment-based drug discovery #FBDD is increasingly viable for membrane proteins using high-throughput #cryoEM. This blog covers cryoEM fragment screening workflows, resolution/occupancy needs for low-MW ligands, and technology advances for structure-guided #drugdiscovery. 👉 ter.li/73g8yx

Engineered nanobodies facilitate cryo-EM studies of small proteins pubmed.ncbi.nlm.nih.gov/41501545/ #cryoem

High-Resolution single particle analysis using a scintillator camera XF416 on CRYOARM300II at 300 kV pubmed.ncbi.nlm.nih.gov/41491063/ #cryoem

Now available: the Proceedings of the 2024 @ccpem.bsky.social em Spring Symposium, including a review of the first ten years of the Symposium: journals.iucr.org/special_issues/2025/CCPEM2024/ View all of the Proceedings at journals.iucr.org/d/services/ccpem

#IUCr2026 Pre-Congress Workshop: Structure Solution with Phenix Offers lectures and tutorials on using Phenix for automated macromolecular structure determination and validation from xtal and cryoEM data, integrating predicted models like AlphaFold event.fourwaves.com/...

We are hiring a specialist for Cryo-EM that will be shared between Harvard FAS and HMS. Check it out! academicpositions.harvard.edu/postings/15677

Hi fellow membrane protein #StructuralBiology people! Do you use maltoside detergents / model them in your stuctures? Watch out! ⚠️ Most PDB models of LMNG and GDN are wrong ❌, yet continue to be re-used. Spread the word! See more: doi.org/10.1021/acsc... (w. @sebvidalchem.bsky.social ) #cryoEM

Very proud to be part of this project! This not only is the (nominally) highest resolution non-ApoF cryoEM structure, but also for the first time resolved a ligand and structural hetetogeneity in that resolution range, giving new insights into the Rubisco enzyme mechanism. A nice start for Krios 5!

Final version is out! Our large-scale cryo-ET dataset 🔬 of Chlamydomonas reinhardtii 🦠 is now published in @cp-molcell.bsky.social Huge collaborative effort! So glad to see the community already using it to develop new resources & tools. Check it out here: shorturl.at/z4i4c #CryoEM #CryoET

Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii pubmed.ncbi.nlm.nih.gov/41421337/ #cryoem

We’re excited to open applications for Cryo-EM in Structural Biology 2026, part of the Wellcome/MRC-funded national cryo-EM training programme! ❄️ 🗓️ Registration is open until 12 January 2026 All the details (and the registration link) are can be found here: astbury.leeds.ac.uk/facilities/w...

CryoSift is a platform-independent convolutional neural network tool for assessing the quality of 2D averages to enable the automatic selection of suitable particles for high-resolution reconstructions #Automation #DataProcessing #CryoEM doi.org/10.1107/S205...

From our software team - #SmartEPU Software v3.14 for #SingleParticleAnalysis. This release gives #cryoEM researchers faster workflows and smarter automation with CryoFlow 2.0. See it in action. ter.li/gfdmj7

Deep learning-based postprocessing and model building for cryo-electron microscopy maps pubmed.ncbi.nlm.nih.gov/41401630/ #cryoem

1.5Å structure of aldolase (150 kDa) from Thermo Fisher's new Krios 5 www.emdataresource.org/EMD-74336

A quick tutorial on the DAQ structure validation score for protein models derived from cryo-EM. DAQ evaluates amino acid–level accuracy in your model. Consider including a DAQ validation report in your next publication! www.youtube.com/watch?v=YRAT...

December Update of DAQ-Score DB! Quality evaluations of protein models from cryo-EM. Now includes evaluation of 258,956 protein chains. Check at daqdb.kiharalab.org It's very easy to compute DAQ for your protein model. Add the validation result in your publication: em.kiharalab.org/algorithm/da...

IsoNet2 determines cellular structures at submolecular resolution without averaging www.biorxiv.org/content/10.64898/2025.12.09.693325v1 #cryoem

An integrated workflow for structural virology with a 100 keV electron microscope www.biorxiv.org/content/10.64898/2025.12.08.693081v1 #cryoem

Happy to share our fun team-up with @centriolelab.bsky.social & @stearnslab.bsky.social to reveal the architecture of the mammalian centriole’s Distal Ring 🛟. Very cool multiscale integration of #UExM 📏🔬with #CryoET ❄️🔬. Congrats to @ebertiaux.bsky.social & @computingcaitie.bsky.social #TeamTomo 🧪

ICYMI: New online: EMProt improves structure determination from cryo-EM maps

Editorial: Breakthroughs in Cryo-EM with machine learning and artificial intelligence pubmed.ncbi.nlm.nih.gov/41358201/ #cryoem

🚨 New blog: GPU-accelerated motion correction Motion correction remains one of the slowest and most costly steps in #cryoEM workflows. So we rebuilt it from scratch. Our new in-house GPU-accelerated CryoCloud Motion Correction is designed for speed, scale and high throughput. lnkd.in/eiJgsYgJ

Visualizing macromolecular machines using cryo-electron microscopy Dr. Lisa Eshun-Wilson ARC Centre for Cryo-EM of Membrane Proteins youtu.be/-eXtdcP3O8k?...

M​atthew D. Giammar et al.: Leopard-EM: an extensible 2D template-matching package to accelerate in situ structural biology ##InSituStructuralBiology##CryoEM##CryoET@usc...##IUCrhttps://journals.iucr.org/paper?S2059798325009982

Jeffrey Gu, Minkyu Jeon, Ambri Ma, Serena Yeung-Levy, Ellen D. Zhong: CryoHype: Reconstructing a thousand cryo-EM structures with transformer-based hypernetworks https://arxiv.org/abs/2512.06332 https://arxiv.org/pdf/2512.06332 https://arxiv.org/html/2512.06332

Since submission, we have a new version of the program with some improvements, and we have many developments in the pipeline for faster, more sensitive template matching. Keep an eye on our GitHub for these. github.com/Lucaslab-Ber...

Now out in full form. Again, a big thanks to #teamtomo and @alisterburt.bsky.social for developing the infrastructure to build this on. journals.iucr.org/d/issues/202...

Mark your calendars for January 13th when our own Alice-Roza Eruera will give a primer on Building CryoET Training Workflows Using the SBGrid Ecosystem. Register here: buff.ly/AaZQlJL #SBGrid #Structuralbiology #Webinars

Leopard-EM: an extensible 2D template-matching package to accelerate in situ structural biology pubmed.ncbi.nlm.nih.gov/41355025/ #cryoem

Just sharing again - don't miss our Webinar on CEMOVIS with a Krios #cryoEM on Tuesday - fantastic insight into both history and recent successes! Register here: ter.li/v0aj7d

Did you know we publish a monthly newsletter? It features Cryo-EM, Crystallography, Beamline and Structural Biology news, information, new product releases and more. Read and subscribe today! zurl.co/glmG #cryoem #crystallography #beamline #structuralbiology

Cryo-IEF: a comprehensive foundation model for image processing in single-particle cryo-EM. www.nature.com/articles/s41...

For our November software webinar we heard from Hui Wang from UCLA on TomoNet - Automatic Particle Picking of Flexible Lattice-Like Structures for CryoET. If you missed it, you can watch it on SBGrid's YouTube channel: buff.ly/gnhR2AV #SBGrid #Webinars

I'm giving a talk at One World Cryo EM (cryoem.world) tomorrow at 11:00am EST. Topic is 'Deconstructing Algorithms for “Discrete” Heterogeneity in Cryo-EM'. Sign up for their mailing list to tune in!

COOT Update: We have added a stand-alone versions of & Coot for Linux and for ARM & Intel-based Macs that are available for academic, non-profit, commercial members to download. These versions will work with MacOS Tahoe & are not dependent on other applications. #SBGrid #SoftwareUpdates

CryoET of microbes inside an animal organ? Yes it’s possible! Check out our new preprint showing how we did it! What an amazing collaboration with amazing scientists who made this possible!