Interested in crystallizing your favorite biomolecule for structural studies? Join the #IUCr2026 Biomolecular Crystallization Workshop for - Sample prep/characterization - Optimization and seeding - Ways to ‘see’ your crystals - Ultrasmall to large crystals - AI/ML advances event.fourwaves.com/...

Have you checked out our latest Interview with Authors? The Editors chat to Mike Cianfrocco, Scott Stagg and Jan-Hannes Schäfer about their CryoSift tool youtu.be/ROlc_eueYfg More interviews at are available at www.youtube.com/playlist?lis...

First issue of 2026 is out: journals.iucr.org/f/issues/202... On the cover: single surface substitutions improve the crystallizability and diffraction properties of a flexible two-domain protein, even if they increase the entropy of the respective residue. Read more at doi.org/10.1107/S205...

M​ichael Weyand et al.: Potential 14-3-3 binding sites in sirtuins reveal extended phosphosite-recognition modes ##Sirtuin##Phosphopeptides##Regulation@unibt...##IUCrhttps://journals.iucr.org/paper?S2053230X25010908

CryoSift is a platform-independent convolutional neural network tool for assessing the quality of 2D averages to enable the automatic selection of suitable particles for high-resolution reconstructions #Automation #DataProcessing #CryoEM doi.org/10.1107/S205...

The rigid single-domain structure and reproducible crystallization behavior of PF1765 from P. furiosus indicate potential use as a fusion domain to aid the crystallization of membrane proteins or complexes #PF1765 #UPF0235 #ProteinCrystallography doi.org/10.1107/S205...

Structural analysis of apo and UDP-bound tetrahydrobiopterin glucosyltransferase revealed features at the active-site pocket differing from other retaining GT-B glycosyltransferases #Tetrahydrobiopterin #GTBGlycosyltransferase #Crystallography doi.org/10.1107/S205...

In the coenzyme-bound structure of short-chain dehydrogenase from Brucella ovis, two different orientations of the NAD+ coenzyme are fortuitously observed #ShortChainDehydrogenaseReductase #ResearchExperienceForUndergraduates doi.org/10.1107/S205...

Thanks to @actacrystf.iucr.org for highlighting our smart 2D selection tool CryoSift. You can test it yourself here cryosift.org

New interview alert! We had a chat with Mike Cianfrocco, Scott Stagg and Jan-Hannes Schäfer about their CryoSift tool – details in the thread. www.youtube.com/watch?v=ROlc...

M​ahima Sharma et al.: Structure of the imine reductase from Ajellomyces dermatitidis in three crystal forms. Corrigendum ##ImineReductase##Biocatalysis@uniofyork...##IUCrhttps://journals.iucr.org/paper?S2053230X25011136

Jacqueline Vitali et al.: Crystal structure of Methanococcus jannaschii dihydroorotase with substrate bound ##Dihydroorotase##MethanococcusJannaschii##PyrimidineBiosynthesis@CLE_State@sunyoldwestbury...##IUCrhttps://journals.iucr.org/paper?S2053230X25010556

Alexander McPherson: A note on the appearance of PEG in macromolecular crystals ##PolyethyleneGlycol##Mechanism##Crystallization@UCIrvine...##IUCrhttps://journals.iucr.org/paper?S2053230X2501043X

Our last issue of 2026 is available: journals.iucr.org/f/issues/202... On the cover: two different orientations of the NAD coenzyme are fortuitously observed in the NAD+-bound structure of a short-chain dehydrogenase from Brucella ovis. Find out more at doi.org/10.1107/S205...

Ahsan Saeed et al.: Crystal structure of Crocus vernus class IIIb chitinase: in silico ligand-binding studies and in vitro antifungal assay ##CrocusVernus##Chitinase##TIM@unihh...##IUCrhttps://journals.iucr.org/paper?S2053230X25009720

Geerds and Niemann: Single mutations to tyrosine or glutamate improve the crystallizability and crystal diffraction properties of a flexible two-domain protein ##ProteinCrystallization@unibielefeld@unibielefeld...##IUCrhttps://journals.iucr.org/paper?S2053230X25010416

Apply for #IUCr2026 travel grants Funds are available to support students, early-career researchers, and participants from under-resourced institutions and/or developing countries. Details at event.fourwaves.com/...

Sujay S. Gaikwad et al.: Crystal structures of PF1765 from Pyrococcus furiosus from several different crystallization conditions with varied pH, salt and precipitant ##PF1765##UPF0235##ProteinCrystallography... ##IUCrhttps://journals.iucr.org/paper?S2053230X25009318

Zang, Jiang and Zhou: Structural analysis of the tetrahydrobiopterin glucosyltransferase PsBGluT from Pseudanabaena sp. Chao 1811 ##Tetrahydrobiopterin##GTBGlycosyltransferase##Crystallography... ##IUCrhttps://journals.iucr.org/paper?S2053230X25009446

A gentle, automated, inexpensive and open-source device and method for changing the ambient solution of a macromolecular crystal #Crystal #Solution #Cracking doi.org/10.1107/S205...

A kinetically impaired Gln143Asn MnSOD variant is used to trap and explore additional hydrogen peroxide-binding sites beyond the second-shell solvent gate #ManganeseSuperoxideDismutases #PeroxideBinding #Metalloenzymes doi.org/10.1107/S205...

Sean P. Zupko et al.: Crystal structure of a short-chain dehydrogenase from Brucella ovis with apo and coenzyme NAD+-bound protomer chains ##RedoxChemistry##ResearchExperienceForUndergraduates@suny_cortland@UMNews...##IUCrhttps://journals.iucr.org/paper?S2053230X25009227

The first crystal structure of FucWf4, a newly characterized fucosidase that has structural similarity to GH29 enzymes but is currently unclassified, is presented #GlycosideHydrolases #Fucoidan #Fucosidase doi.org/10.1107/S205...

Structural investigations of unliganded murine KHK-A revealed the adoption of two conformations similar to those adopted by the human ortholog, suggesting this structural feature is conserved across species #PfkBFamily #Moonlighting #ProteinKinases doi.org/10.1107/S205...

Jan-Hannes Schäfer et al.: CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection ##Automation##DataProcessing##CryoEM@umich...##IUCrhttps://journals.iucr.org/paper?S2053230X25008866

CryoSift is now out @actacrystf.iucr.org doi.org/10.1107/S205... Thanks to all involved. Happy processing!

November issue out now: journals.iucr.org/f/issues/202... On the cover: the structure of the kinetically slow and catalytically impaired Gln143Asn mutant of human mitochondrial MnSOD leads to the observation of transient hydrogen peroxide-bound states. More at shorturl.at/41Mb9

M​edhanjali Dasgupta et al.: High-resolution X-ray structure of Gln143Asn manganese superoxide dismutase captures multiple hydrogen peroxide-binding sites ##Metalloenzymes##PeroxideBinding@unmc...##IUCrhttps://journals.iucr.org/paper?S2053230X25009045

One of the coolest things going on here is that the GitHub repos contain Jupyter notebooks for you to try their software. In case you don't have Jupyter installed or don't know what it is: you can always upload the notebooks to Google Colab and run everything on their cloud services :)

Tools are presented that are useful for collating existing public cryoEM data sets and/or creating new synthetic cryoEM data sets to aid the development of novel data processing and interpretation algorithms #CryoEM #ElectronCryomicroscopy #Python doi.org/10.1107/S205...

D-Aspartate oxidase from Cryptococcus humicola UJ1 reveals a unique homotetrameric organization due to distinct interfacial loop arrangements #DAspartateOxidase #CrystalStructure #FADDependentEnzymes doi.org/10.1107/S205...

Brooke Bailey et al.: Structural insights into a fucosidase involved in fucoidan degradation ##GlycosideHydrolases##Fucoidan##Fucosidase... ##IUCrhttps://journals.iucr.org/paper?S2053230X25008842

The structure of B. subtilis DegQ from the DegS–DegU two-component system provide structural insight into DegQ oligomerization and its potential role in modulating DegS autophosphorylation and DegU binding #BacillusSubtilis #CrystalStructure #DegQ doi.org/10.1107/S205...

Bae, Allen and Tolan: Conformational changes in ketohexokinase are conserved across isozymes and species ##PfkBFamily##Moonlighting##ProteinKinases... ##IUCrhttps://journals.iucr.org/paper?S2053230X25008428

Great to see this article by @guptapenn.bsky.social is out! Kushol helped us interview @xtalmaker.bsky.social Vivian Stojanoff and @gabbygrowsxtals.bsky.social who published an article on careful sample preparation in crystallisation experiments, also part of #SAMPREP www.youtube.com/watch?v=m34R...

Four cyanobacterial glycosyl transferase family 35 α-glucan phosphorylases of types I and II were biochemically characterized and one was crystallized and crystallographically analyzed #Cyanobacteria #CyanobacterialStarch #Glycogen doi.org/10.1107/S205...

The higher resolution, significantly improved apo crystal structure of mouse gasdermin D will be beneficial for structure-based drug-design approaches towards this important pharmacological target #Pyroptosis #Inflammation #GasderminD doi.org/10.1107/S205...

The SAMPREP workshop at the 2023 ACA Annual Meeting in Baltimore was designed to address the need to consoiidate cross-disciplinary best practices for sample preparation into a single forum #SamplePreparation #StructuralBiology #CryoEM doi.org/10.1107/S205...

Take a look at our October issue: journals.iucr.org/f/issues/202... The cover shows the crystal structure of Cryptococcus humicola D-aspartate oxidase, which has a unique homotetrameric architecture in contrast to other known D-amino-acid oxidases. Read more at doi.org/10.1107/S205...

D​ouglas H. Juers et al.: Automated gradient equilibration of macromolecular crystals to new solution conditions ##Crystal##Solution##Cracking... ##IUCrhttps://journals.iucr.org/paper?S2053230X25008398

Take a look at our October issue: journals.iucr.org/f/issues/202... The cover shows the crystal structure of Cryptococcus humicola D-aspartate oxidase, which has a unique homotetrameric architecture in contrast to other known D-amino-acid oxidases. Read more at doi.org/10.1107/S205...

I'm incredibly proud to present this @actacrystf.iucr.org interview with Alex Mendez and Cydni Bolling on their first paper ever! The interview was co-led by Dr Graham Chakafana from Hampton University in the USA, who supervised the authors' undergraduate research. www.youtube.com/watch?v=5rjI...

The structure of a 7-substitution mutant of hydroxynitrile lyase shows that targeted amino-acid substitutions can shift catalytic geometries towards those of evolutionarily related enzymes #EngineeredProteins #HydroxynitrileLyases #HydrolaseFold doi.org/10.1107/S205...

The World Directory of Crystallographers (WDC) offers an unparalleled compilation of expertise in the field. It’s free to join and open to all crystallographers and other scientists employing crystallographic methods. Add your name and be part of the mosaic: 🔗 www.iucr.org/people/wdc

Crystal structures of a clinically used histone deacetylase inhibitor, vorinostat, bound to the previously unidentified off-target proteins carbonic anhydrases II and IX are reported #HDACInhibitors #SAHA #Vorinostat doi.org/10.1107/S205...

The crystal structure of Fasciola hepatica TPI has been solved at 1.51 Å and used to perform molecular-docking studies with the most successful fasciolocide triclabendazole #FasciolaHepatica #TriosephosphateIsomerase #TPI doi.org/10.1107/S205...

Virulence protein J domain 1 maintains the canonical α/β-hydrolase fold but is likely to lack a functional catalytic triad, suggesting functional divergence from classical hydrolases of this superfamily #BacterialPathogens #TypeIVSecretionSystem doi.org/10.1107/S205...

The first high-resolution structure of the folded domains of Xrs2 from S. cerevisiae will advance understanding of its functions in the DNA damage response and telomere-length maintenance @UMNews #Nbs1Xrs2 #DNADoubleStrandBreaks #DNADamageRepair doi.org/10.1107/S205...

Our September issue is available! tinyurl.com/58x22mpx The cover shows the crystal structure of the N-terminal domain 1 of virulence protein J from Brucella abortus, which adopts an α/β-hydrolase fold but lacks the characteristic catalytic triad: see tinyurl.com/bdduy23d

📣Scholarship program of the European Crystallographic Association for PhD students or young post-docs working in any field of crystallography!!! Apply before October 15, 2025!!! More info: ecanews.org/blog/2025/09...