An acylated GLP-1/GIP analogue peptide forms a uniquely porous spiral crystal structure composed of clockwise-ascending monomers in a square pattern #GLP1 #Peptides #Crystallization doi.org/10.1107/S205...

A comprehensive survey catalogues all pentatricopeptide repeat protein crystal structures, analysing their crystallographic properties, structural features and contributions to understanding function #PentatricopeptideRepeatProteins #RNARecognition doi.org/10.1107/S205...

Our April issue is out now! journals.iucr.org/f/issues/202... Crystallization was investigated as a preparative strategy for a GLP-1/GIP lipopeptide and led to what is believed to be the first reported crystal structure of an unbound GLP-1/GIP analogue doi.org/10.1107/S205...

Abe, Fukui and Ogura: Crystal structure of PM1Pgh, a poly-γ-glutamate hydrolase from Bacillus phage PM1 ##PolyGlutamateHydrolases##Pgh##BacteriophagePM1@NaraWomensUniv...##IUCrhttps://journals.iucr.org/paper?S2053230X26002189

The structure of endo-β-N-acetylglucosaminidase was determined at 1.8 Å resolution, revealing a unique spatial arrangement of domain IV, which is suggested to contribute to substrate recognition #EndoNAcetylglucosaminidases #GH85 #ComplexNGlycans doi.org/10.1107/S205...

The structure of a chimeric efflux pump was determined by cryoEM under apo and kanamycin-supplemented conditions and adopted symmetric-like and asymmetric conformations #PseudomonasAeruginosa #DrugEffluxTransporters #MembraneProteins doi.org/10.1107/S205...

Cooper and Isom: Optimized bacterial expression of a synthetic BRIL antibody ##CryoEM##XRayCrystallography##BRIL@UniofOxford@UniofOxford...##IUCrhttps://journals.iucr.org/paper?S2053230X26001548

MBP fusion is an effective and generalizable approach to enable crystallographic studies of engineered binding proteins that are otherwise difficult to crystallize #Monobody #Aggregation #MaltoseBindingProtein doi.org/10.1107/S205...

M​. Y. Stoliarskaia et al.: X-ray analysis of complexes of carbonic anhydrase II with 1,3-oxazole-containing sulfonamide derivatives elucidates the structural basis for their exceptionally high inhibitory potency ... ##IUCrhttps://journals.iucr.org/paper?S2053230X26002128

This report of the structure of a carbon-nitrogen hydrolase doi.org/10.1107/S205... is the first contribution to the Early Career Scientists in Structural Science special issue! Find out more, join in and submit at journals.iucr.org/early-career...

Here's our March issue: journals.iucr.org/f/issues/202... On the cover: the structure of the chimeric RND-type multidrug-efflux pump protein MexBYB was determined by cryoEM under apo and kanamycin-supplemented conditions journals.iucr.org/f/issues/202...

The Protein Data Bank (#PDB) has reached a new milestone: 250,441 entries in the archive, a record since its founding in 1971, all thanks to the efforts and contributions of the structural biology community. ⬇️ more ⬇️ www.wwpdb.org/news/news?ye... #StructuralBiology

Ikuya Kurauchi et al.: Crystal structure of endo-β-N-acetylglucosaminidase HSα ##EndoNAcetylglucosaminidases##GH85##ComplexNGlycans... ##IUCrhttps://journals.iucr.org/paper?S2053230X26001214

Jiye Wang et al.: Cryo-EM structures of a MexB–MexY chimeric efflux pump reveal that large open clefts are intrinsic to the MexY porter domain ##MembraneProteins##DrugEffluxTransporters@osaka_univ_e@UTokyo_News...##IUCrhttps://journals.iucr.org/paper?S2053230X26001202

This structure of a tRNA acceptor-stem mimic demonstrates the use of bromine and phosphorus anomalous scattering for RNA structure determination #Crystallography #AnomalousScattering #NucleicAcids doi.org/10.1107/S205...

Aruesha Srivastava et al.: Crystal structure of carbon–nitrogen hydrolase from Helicobacter pylori G27 ##Ssgcid##StructuralGenomics##HelicobacterPylori@_hamptonu...##IUCrhttps://journals.iucr.org/paper?S2053230X26001330

Sample preparation from α- and β-crustacyanin pigments purified from ex vivo material from the American lobster Homarus americanus for X-ray crystallography and cryo-electron microscopy studies #Astaxanthin #Crustacyanin #Absorption doi.org/10.1107/S205...

The highest resolution DNA-free TaXPD structure to date uniquely resolves loop 510–514 in the long-form, DNA-free TaXPD model #XRayCrystallography #Helicases #Archaea doi.org/10.1107/S205...

Potential 14-3-3 binding sites in sirtuins were identfied and the interactions of peptides representing phosphorylation sites in sirtuin 3 and sirtuin 1 with 14-3-3 proteins were characterized #Sirtuin #Phosphopeptides #Regulation doi.org/10.1107/S205...

The flexible loop in Methanococcus jannaschii dihydroorotase with substrate bound adopts both the loop-out and loop-in conformations #Dihydroorotase #MethanococcusJannaschii #PyrimidineBiosynthesis doi.org/10.1107/S205...

The crystal structure of the class IIIb chitinase from Crocus vernus is reported, together with in silico analyses of inhibitor interactions and antifungal and chitinase assays #CrocusVernus #Chitinase #TIM doi.org/10.1107/S205...

Single surface substitutions can mprove the crystallizability and diffraction properties of a flexible two-domain protein even if they increase the entropy of the residue #MultidomainProtein #PointMutation #ProteinCrystallization doi.org/10.1107/S205...

Koushirou Endo et al.: Crystallization and X-ray structure of a highly aggregation-prone monobody engineered for high-affinity small-molecule recognition ##Monobody##Aggregation##MaltoseBindingProtein@nitechofficial...##IUCrhttps://journals.iucr.org/paper?S2053230X26000798

What are the effects of the presence of polyethylene glycol in macromolecular crystals? #PolyethyleneGlycol #Mechanism #Crystallization doi.org/10.1107/S205...

February issue now available: journals.iucr.org/f/issues/202... The cover shows the crystal structure of an RNA complex composed of four strands determined by the complementary use of bromine and phosphorus anomalous signals. Find out more at doi.org/10.1107/S205...

Ziwei Liu et al.: Crystal structure of a tRNA acceptor-stem mimic at 1.94 Å resolution ##Crystallography##AnomalousScattering##NucleicAcids@Cambridge_Uni...##IUCrhttps://journals.iucr.org/paper?S2053230X26000658

M​aria Claudia Cedri et al.: Sample purification and characterization of the α- and β-crustacyanin pigments from the American lobster for crystallographic and cryo-EM studies ##Astaxanthin##Crustacyanin##Absorption... ##IUCrhttps://journals.iucr.org/paper?S2053230X2600018X

Interested in crystallizing your favorite biomolecule for structural studies? Join the #IUCr2026 Biomolecular Crystallization Workshop for - Sample prep/characterization - Optimization and seeding - Ways to ‘see’ your crystals - Ultrasmall to large crystals - AI/ML advances event.fourwaves.com/...

Have you checked out our latest Interview with Authors? The Editors chat to Mike Cianfrocco, Scott Stagg and Jan-Hannes Schäfer about their CryoSift tool youtu.be/ROlc_eueYfg More interviews at are available at www.youtube.com/playlist?lis...

First issue of 2026 is out: journals.iucr.org/f/issues/202... On the cover: single surface substitutions improve the crystallizability and diffraction properties of a flexible two-domain protein, even if they increase the entropy of the respective residue. Read more at doi.org/10.1107/S205...

M​ichael Weyand et al.: Potential 14-3-3 binding sites in sirtuins reveal extended phosphosite-recognition modes ##Sirtuin##Phosphopeptides##Regulation@unibt...##IUCrhttps://journals.iucr.org/paper?S2053230X25010908

CryoSift is a platform-independent convolutional neural network tool for assessing the quality of 2D averages to enable the automatic selection of suitable particles for high-resolution reconstructions #Automation #DataProcessing #CryoEM doi.org/10.1107/S205...

The rigid single-domain structure and reproducible crystallization behavior of PF1765 from P. furiosus indicate potential use as a fusion domain to aid the crystallization of membrane proteins or complexes #PF1765 #UPF0235 #ProteinCrystallography doi.org/10.1107/S205...

Structural analysis of apo and UDP-bound tetrahydrobiopterin glucosyltransferase revealed features at the active-site pocket differing from other retaining GT-B glycosyltransferases #Tetrahydrobiopterin #GTBGlycosyltransferase #Crystallography doi.org/10.1107/S205...

In the coenzyme-bound structure of short-chain dehydrogenase from Brucella ovis, two different orientations of the NAD+ coenzyme are fortuitously observed #ShortChainDehydrogenaseReductase #ResearchExperienceForUndergraduates doi.org/10.1107/S205...

Thanks to @actacrystf.iucr.org for highlighting our smart 2D selection tool CryoSift. You can test it yourself here cryosift.org

New interview alert! We had a chat with Mike Cianfrocco, Scott Stagg and Jan-Hannes Schäfer about their CryoSift tool – details in the thread. www.youtube.com/watch?v=ROlc...

M​ahima Sharma et al.: Structure of the imine reductase from Ajellomyces dermatitidis in three crystal forms. Corrigendum ##ImineReductase##Biocatalysis@uniofyork...##IUCrhttps://journals.iucr.org/paper?S2053230X25011136

Jacqueline Vitali et al.: Crystal structure of Methanococcus jannaschii dihydroorotase with substrate bound ##Dihydroorotase##MethanococcusJannaschii##PyrimidineBiosynthesis@CLE_State@sunyoldwestbury...##IUCrhttps://journals.iucr.org/paper?S2053230X25010556

Alexander McPherson: A note on the appearance of PEG in macromolecular crystals ##PolyethyleneGlycol##Mechanism##Crystallization@UCIrvine...##IUCrhttps://journals.iucr.org/paper?S2053230X2501043X

Our last issue of 2026 is available: journals.iucr.org/f/issues/202... On the cover: two different orientations of the NAD coenzyme are fortuitously observed in the NAD+-bound structure of a short-chain dehydrogenase from Brucella ovis. Find out more at doi.org/10.1107/S205...

Ahsan Saeed et al.: Crystal structure of Crocus vernus class IIIb chitinase: in silico ligand-binding studies and in vitro antifungal assay ##CrocusVernus##Chitinase##TIM@unihh...##IUCrhttps://journals.iucr.org/paper?S2053230X25009720

Geerds and Niemann: Single mutations to tyrosine or glutamate improve the crystallizability and crystal diffraction properties of a flexible two-domain protein ##ProteinCrystallization@unibielefeld@unibielefeld...##IUCrhttps://journals.iucr.org/paper?S2053230X25010416

Apply for #IUCr2026 travel grants Funds are available to support students, early-career researchers, and participants from under-resourced institutions and/or developing countries. Details at event.fourwaves.com/...

Sujay S. Gaikwad et al.: Crystal structures of PF1765 from Pyrococcus furiosus from several different crystallization conditions with varied pH, salt and precipitant ##PF1765##UPF0235##ProteinCrystallography... ##IUCrhttps://journals.iucr.org/paper?S2053230X25009318

Zang, Jiang and Zhou: Structural analysis of the tetrahydrobiopterin glucosyltransferase PsBGluT from Pseudanabaena sp. Chao 1811 ##Tetrahydrobiopterin##GTBGlycosyltransferase##Crystallography... ##IUCrhttps://journals.iucr.org/paper?S2053230X25009446

A gentle, automated, inexpensive and open-source device and method for changing the ambient solution of a macromolecular crystal #Crystal #Solution #Cracking doi.org/10.1107/S205...

A kinetically impaired Gln143Asn MnSOD variant is used to trap and explore additional hydrogen peroxide-binding sites beyond the second-shell solvent gate #ManganeseSuperoxideDismutases #PeroxideBinding #Metalloenzymes doi.org/10.1107/S205...

Sean P. Zupko et al.: Crystal structure of a short-chain dehydrogenase from Brucella ovis with apo and coenzyme NAD+-bound protomer chains ##RedoxChemistry##ResearchExperienceForUndergraduates@suny_cortland@UMNews...##IUCrhttps://journals.iucr.org/paper?S2053230X25009227

The first crystal structure of FucWf4, a newly characterized fucosidase that has structural similarity to GH29 enzymes but is currently unclassified, is presented #GlycosideHydrolases #Fucoidan #Fucosidase doi.org/10.1107/S205...