Happy to share our new review on 3D chromatin organisation by cohesin with you. It was a great pleasure to review recent advances and future prospects with @danielgerlich.bsky.social.

CAD-C: An engineered nuclease enables repair-free in situ proximity ligation and nucleosome-resolution chromosome walks in human cells https://www.biorxiv.org/content/10.64898/2025.12.22.695891v1

🚨Our work on the impact of DNA replication on 3D genome is out in Genome Biology: replication-dependent loop extrusion by sister-forks, wave of replication, no evidence for large-scale replication factory. Great collab with @aurelepiazza.bsky.social. More here: link.springer.com/article/10.1...

A thread on our latest paper from the Whitehouse lab

Hi all! We developed CAD-C to map chromatin organization at high res in yeast. It revealed an unexpected tight alignment: nucleosomes covering the same sequence tend to be associated across sister chromatids. www.biorxiv.org/content/10.6... #3DGenome #chromatin #Cohesin #centromere

CAD-C reveals centromere pairing and near-perfect alignment of sister chromatids https://www.biorxiv.org/content/10.64898/2025.12.18.694906v1

Hello everybody, I am looking for an Engineer (IE) to help me start the lab at LBMC / ENS-Lyon in April. We will investigate chromatin organization during replication in yeast. RT appreciated! To apply: inserm.softy.pro/offre/187863 @lbmcinlyon.bsky.social #NewPI #3DGenome #chromatin #replication

Hi all ! Happy to share that I will open a lab at the LBMC at ENS-Lyon thanks to the #ATIP-Avenir funding. We will investigate the role the 3D chromatin organization in Replication and Repair. www.ens-lyon.fr/LBMC/equipes...

 Job offers out soon! #NewPI #3DGenome #chromatin #replication

To probe gene-scale chromatin physics, we built 96-mer (20 kb) arrays with defined histone marks. Combining single-molecule tracking, AFM imaging, and developing in vitro Hi-C, we saw how specific modifications dictate chromatin structure and dynamics. www.science.org/doi/10.1126/...

Last reminder: We’ll see you bright and early at 9:30 AM EST on the 25th to kick off #chromatin2025! Fantastic lineup of speakers with discussion to follow each talk. Don’t forget to register for this virtual meeting if you haven’t yet: bit.ly/chromatin-2025

Our latest paper has just been published in Cell! doi.org/10.1016/j.ce... We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.

Happy to share our latest publication, in which we show that the arrangement of nucleosomes around CTCF sites contributes to higher-order organisation of chromatin into TADs: www.embopress.org/doi/full/10....

BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)

Today I am so pleased to present our work on how chromatin remodelers affect mesoscale chromatin organization. www.science.org/doi/10.1126/...

New preprint from the lab !! Loop extrusion may provide mechanical robustness to chromatin. Great work by Hossein Salari. @cnrs.fr @lbmcinlyon.bsky.social www.biorxiv.org/content/10.1...

Thrilled that our work is now finally out in Nature Comms! rdcu.be/eG3vP We reveal cryo-EM structures of the MRN complex bound to DNA & TRF2 - showing how DNA breaks are sensed and regulated at telomeres. Fantastic work by first authors @yilanfan.bsky.social @filizkuybu.bsky.social & Hengjun!

Congrats to my friends in the Boettiger lab for this really beautiful live imaging work. A big leap forward in understanding the dynamic side of genome organization. www.science.org/doi/10.1126/...

Our new preprint is out＠bioRxiv: www.biorxiv.org/content/10.1... @masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

We’re back! Registration is NOW OPEN for the 6th Annual Meeting on Advances in Nuclear Topology & 3D Chromatin Architecture in Cancer. Don’t miss insights from leading experts in the field. Join us online on Nov 25, 2025—register for free: bit.ly/chromatin-2025 #chromatin2025

Checkout our latest research in @natcomms.nature.com rdcu.be/eBqBI A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells. A collaboration with Mike Boemo’s team

Transcription and recombination are two universal DNA-dependent processes, but how they are coordinated remains largely unknown. Here we characterized transcription-recombination priority rules in yeast. 🧵 www.embopress.org/doi/full/10....

Contributions of local structural and energetic features of DNA to large-scale genomic organization [perspective by Wilma Olson & Co) www.sciencedirect.com/science/arti...

Activity of most genes is controlled by multiple enhancers, but is there activation coordinated? We leveraged Nanopore to identify a specific set of elements that are simultaneously accessible on the same DNA molecules and are coordinated in their activation. www.biorxiv.org/content/10.1...

New work from @beckof.bsky.social and our lab's collaboration, with @alengronne.bsky.social! Multifaceted influence of transcription on yeast chromosome folding... loop extrusion of course but also active promoters making loops, bypassing centromeres boundaries www.sciencedirect.com/science/arti...

New topology Pre-print from: @alengronne.bsky.social Condensin and topoisomerases cooperate to relieve topological stress at stalled replication forks: biorxiv.org/content/earl...

Very excited to share the first preprint of the lab! We show that the nuclear pore protein TPR forms biomolecular condensates which keep heterochromatin away from the nuclear pore complexes and maintains global chromatin organization. Check it out, share it, tell us what you think!!

Our paper is now out in Molecular Cell! Check the thread in this former post: www.cell.com/molecular-ce...

@morielab.bsky.social proudly presents a tool to induce, in S. cerevisiae, DNA double strand breaks in a site-specific AND in a dose-dependent manner, in a very simple way: just transform two plasmids!!! It allows you to assess many aspects of DNA damage signaling & repair! doi.org/10.1111/febs...

Hi everyone! The latest method from the Whitehouse lab: PCP 
PCP uses a novel proximity barcoding strategy to simultaneously map 3D genome organization at various resolutions at the single molecule level: Out soon, check the updated BioRxiv – little thread: 1/11 www.biorxiv.org/content/10.1...

Join us for the 2025 GRC Genome Architecture www.grc.org/genome-archi... @spp2202.bsky.social @jobdekker.bsky.social @metorrespadilla.bsky.social @jopkind.bsky.social @dimplenotani.bsky.social @stanleyqilab.bsky.social @andybelmont.bsky.social @fondufemittendorf.bsky.social @boyanbonev.bsky.social

Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social, @marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social 🔗 Preprint here tinyurl.com/3a74uanv

Excellent thread on the paper from Meneu, Chapard et al. The latest from the Koszul and friends lab! www.science.org/doi/10.1126/...

Thrilled to share first part of my post-doc work, published today in @science.org www.science.org/doi/10.1126/... We use reconstituted replication system and cryo-EM to unravel an unexpected mechanism of DNA translocation employed by eukaryotic replicative helicase.

How do replisomes walk on DNA? And what happens when they run into a G-quadruplex? @sahilbatra.bsky.social and @benallwein.bsky.social provide unexpected insight in our latest paper with Richard Hite @mskcancercenter.bsky.social @science.org www.science.org/doi/10.1126/.... Congrats to all authors!

Roche SBX preprint out www.biorxiv.org/content/10.1...

Our latest work: how can compartmentalization emerge in a eukaryotic genome lacking canonical heterochromatin? By investigating bacterial genomes put in yeast, we show that the presence or absence of transcription is sufficient! #chromatin #3Dgenome #generegulation www.science.org/doi/10.1126/... 👇

Thermodynamic principles link in vitro transcription factor affinities to single-molecule chromatin states in cells www.biorxiv.org/content/10.1... ▶️40-fold flanking sequence effect on TF binding ▶️Motif recognition beats kinetics ▶️Minutes-long residence times ▶️Models of TF-nucleosome competiton

What is the multiscale structure of chromatin condensates? How does it shape thermodynamic and material properties? We address this at near-atomistic resolution🔥🔥🔥 using cryoET (Rosen & Villa labs, led by H Zhou), a new multiscale model (K Russell) and cryoET-guided sims (J Huertas & J Maristany)

Super excited to share our new study from the @jbuenrostro.bsky.social Lab in @nature.com! We developed a computational method for tracking transcription factor and nucleosome binding using single-cell ATAC-seq and deep learning. Paper: www.nature.com/articles/s41...

Multiscale footprints reveal the organization of cis-regulatory elements www.nature.com/articles/s41... ▶️Footprints of DNA–protein interactions from bulk & single-cell ATAC-seq ⤵️ ▶️Deep learning to infer transcription factor and nucleosome binding ▶️Age-associated reduction of nucleosome footprints

Do you have a new and exciting chromatin/transcription story that you'd like to share with an enthusiastic audience? If so, you should apply to give a talk in the 2025 Fragile Nucleosome seminar series: forms.gle/fwMYPFDf4az3...! We're open to scientists at all stages of their careers!

New discovery from our #CDlab reported in Cell @cp-cell.bsky.social today: SMC motor proteins have a left-right ‘gearbox’ and can switch direction in DNA loop extrusion! www.sciencedirect.com/science/arti... 1/

For my first post here at 🦋, let me tell you about a lovely paper by Richard Koche, @anton-henssen.bsky.social, and colleagues that covers how extrachromosomal DNA (ecDNA) drives cancer genome remodeling in neuroblastoma. It's a great story about cancer biology and genome organization! 🧬

bsky.app/profile/did:... A 3D genome feed if anyone is interested

Interesting new method to map nucleosomes in 3D, "Proximity Copy Paste (PCP)". www.biorxiv.org/content/10.1... ▶️ mapped connectivity of nucleosomes in S. cerevisiae ▶️ chromatin is predominantly organized into regularly spaced nucleosome arrays ▶️ metaphase chromosomes packed by arrayed cohesin hubs

Next, if you're looking for your daily hit of posts that focus on gene regulation, here's a relevant feed: bsky.app/profile/shau... The way the feed works is that it searches for posts containing #GeneReg or a set of keywords (see next post).

Hi Bluesky! I made a 3D chromatin starter pack. Let me know if you would like to be added. go.bsky.app/6tTQdqQ

Interested in chromatin architecture?
 Checkout our new method - PCP - that can map genome organization as multi-way interactions as well as nucleosome footprint and nucleosome spacing at very high resolution. www.biorxiv.org/content/10.1...

Putting together packs of transcription and Chromatin people - Nominate others to add! go.bsky.app/5zgpZfg