Thrilled that our work is now finally out in Nature Comms! rdcu.be/eG3vP We reveal cryo-EM structures of the MRN complex bound to DNA & TRF2 - showing how DNA breaks are sensed and regulated at telomeres. Fantastic work by first authors @yilanfan.bsky.social @filizkuybu.bsky.social & Hengjun!

Congrats to my friends in the Boettiger lab for this really beautiful live imaging work. A big leap forward in understanding the dynamic side of genome organization. www.science.org/doi/10.1126/...

Our new preprint is out＠bioRxiv: www.biorxiv.org/content/10.1... @masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

We’re back! Registration is NOW OPEN for the 6th Annual Meeting on Advances in Nuclear Topology & 3D Chromatin Architecture in Cancer. Don’t miss insights from leading experts in the field. Join us online on Nov 25, 2025—register for free: bit.ly/chromatin-2025 #chromatin2025

Checkout our latest research in @natcomms.nature.com rdcu.be/eBqBI A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells. A collaboration with Mike Boemo’s team

Transcription and recombination are two universal DNA-dependent processes, but how they are coordinated remains largely unknown. Here we characterized transcription-recombination priority rules in yeast. 🧵 www.embopress.org/doi/full/10....

Contributions of local structural and energetic features of DNA to large-scale genomic organization [perspective by Wilma Olson & Co) www.sciencedirect.com/science/arti...

Activity of most genes is controlled by multiple enhancers, but is there activation coordinated? We leveraged Nanopore to identify a specific set of elements that are simultaneously accessible on the same DNA molecules and are coordinated in their activation. www.biorxiv.org/content/10.1...

New work from @beckof.bsky.social and our lab's collaboration, with @alengronne.bsky.social! Multifaceted influence of transcription on yeast chromosome folding... loop extrusion of course but also active promoters making loops, bypassing centromeres boundaries www.sciencedirect.com/science/arti...

New topology Pre-print from: @alengronne.bsky.social Condensin and topoisomerases cooperate to relieve topological stress at stalled replication forks: biorxiv.org/content/earl...

Very excited to share the first preprint of the lab! We show that the nuclear pore protein TPR forms biomolecular condensates which keep heterochromatin away from the nuclear pore complexes and maintains global chromatin organization. Check it out, share it, tell us what you think!!

Our paper is now out in Molecular Cell! Check the thread in this former post: www.cell.com/molecular-ce...

@morielab.bsky.social proudly presents a tool to induce, in S. cerevisiae, DNA double strand breaks in a site-specific AND in a dose-dependent manner, in a very simple way: just transform two plasmids!!! It allows you to assess many aspects of DNA damage signaling & repair! doi.org/10.1111/febs...

Hi everyone! The latest method from the Whitehouse lab: PCP 
PCP uses a novel proximity barcoding strategy to simultaneously map 3D genome organization at various resolutions at the single molecule level: Out soon, check the updated BioRxiv – little thread: 1/11 www.biorxiv.org/content/10.1...

Join us for the 2025 GRC Genome Architecture www.grc.org/genome-archi... @spp2202.bsky.social @jobdekker.bsky.social @metorrespadilla.bsky.social @jopkind.bsky.social @dimplenotani.bsky.social @stanleyqilab.bsky.social @andybelmont.bsky.social @fondufemittendorf.bsky.social @boyanbonev.bsky.social

Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social, @marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social 🔗 Preprint here tinyurl.com/3a74uanv

Excellent thread on the paper from Meneu, Chapard et al. The latest from the Koszul and friends lab! www.science.org/doi/10.1126/...

Thrilled to share first part of my post-doc work, published today in @science.org www.science.org/doi/10.1126/... We use reconstituted replication system and cryo-EM to unravel an unexpected mechanism of DNA translocation employed by eukaryotic replicative helicase.

How do replisomes walk on DNA? And what happens when they run into a G-quadruplex? @sahilbatra.bsky.social and @benallwein.bsky.social provide unexpected insight in our latest paper with Richard Hite @mskcancercenter.bsky.social @science.org www.science.org/doi/10.1126/.... Congrats to all authors!

Roche SBX preprint out www.biorxiv.org/content/10.1...

Our latest work: how can compartmentalization emerge in a eukaryotic genome lacking canonical heterochromatin? By investigating bacterial genomes put in yeast, we show that the presence or absence of transcription is sufficient! #chromatin #3Dgenome #generegulation www.science.org/doi/10.1126/... 👇

Thermodynamic principles link in vitro transcription factor affinities to single-molecule chromatin states in cells www.biorxiv.org/content/10.1... ▶️40-fold flanking sequence effect on TF binding ▶️Motif recognition beats kinetics ▶️Minutes-long residence times ▶️Models of TF-nucleosome competiton

What is the multiscale structure of chromatin condensates? How does it shape thermodynamic and material properties? We address this at near-atomistic resolution🔥🔥🔥 using cryoET (Rosen & Villa labs, led by H Zhou), a new multiscale model (K Russell) and cryoET-guided sims (J Huertas & J Maristany)

Super excited to share our new study from the @jbuenrostro.bsky.social Lab in @nature.com! We developed a computational method for tracking transcription factor and nucleosome binding using single-cell ATAC-seq and deep learning. Paper: www.nature.com/articles/s41...

Multiscale footprints reveal the organization of cis-regulatory elements www.nature.com/articles/s41... ▶️Footprints of DNA–protein interactions from bulk & single-cell ATAC-seq ⤵️ ▶️Deep learning to infer transcription factor and nucleosome binding ▶️Age-associated reduction of nucleosome footprints

Do you have a new and exciting chromatin/transcription story that you'd like to share with an enthusiastic audience? If so, you should apply to give a talk in the 2025 Fragile Nucleosome seminar series: forms.gle/fwMYPFDf4az3...! We're open to scientists at all stages of their careers!

New discovery from our #CDlab reported in Cell @cp-cell.bsky.social today: SMC motor proteins have a left-right ‘gearbox’ and can switch direction in DNA loop extrusion! www.sciencedirect.com/science/arti... 1/

For my first post here at 🦋, let me tell you about a lovely paper by Richard Koche, @anton-henssen.bsky.social, and colleagues that covers how extrachromosomal DNA (ecDNA) drives cancer genome remodeling in neuroblastoma. It's a great story about cancer biology and genome organization! 🧬

bsky.app/profile/did:... A 3D genome feed if anyone is interested

Interesting new method to map nucleosomes in 3D, "Proximity Copy Paste (PCP)". www.biorxiv.org/content/10.1... ▶️ mapped connectivity of nucleosomes in S. cerevisiae ▶️ chromatin is predominantly organized into regularly spaced nucleosome arrays ▶️ metaphase chromosomes packed by arrayed cohesin hubs

Next, if you're looking for your daily hit of posts that focus on gene regulation, here's a relevant feed: bsky.app/profile/shau... The way the feed works is that it searches for posts containing #GeneReg or a set of keywords (see next post).

Hi Bluesky! I made a 3D chromatin starter pack. Let me know if you would like to be added. go.bsky.app/6tTQdqQ

Interested in chromatin architecture?
 Checkout our new method - PCP - that can map genome organization as multi-way interactions as well as nucleosome footprint and nucleosome spacing at very high resolution. www.biorxiv.org/content/10.1...

Putting together packs of transcription and Chromatin people - Nominate others to add! go.bsky.app/5zgpZfg

ok, I tried to make a starter pack on genome stability/DNA replication/DNA repair etc. Sorry if I couldn't find someone, please nominate yourself and your colleagues in comments or DMs, looking forward to connecting with everyone! go.bsky.app/9jdDNxG

My first post here! Everything you always wanted to know about Sen1 (but were afraid to dig through papers for...) Here: doi.org/10.1016/j.jm...

Today!

At last, there's a searchable directory of Bluesky starter packs, courtesy of @mubashariqbal.com! Just one more way third-party developers are helping to make this place better. Search starter packs by keyword, and sort by "Uses" to see which ones people are finding the most helpful

Cool lineup next Monday at NYU on 3D chromatin architecture and cancer. Can follow on zoom here: nyulangone.zoom.us/webinar/regi...

In our most recent episode we talked with Carl Wu from Johns Hopkins University about his work on nucleosome remodeling, histone variants, and the role of single-molecule imaging in gene regulation. #epigenetics #podcast Listen here: activemotif.com/podcasts-car...

Another tip for Bluesky newbies - no, it's not as fast-paced here, immediate engagement - here, YOU are the algorithm so you need to engage! Post, comment, re-post others, follow lots to start with - engagement is the key to traction and interaction here. Be proactive, not passive. Well worth it!

Welcome, new followers and comrades. Good advice here. Found it on #academicsky

www.nature.com/articles/s41... Cool work by Jamie Phipps and the Dubrana lab, showing a role for Cohesin in end-tethering in DSB repair

Replisome passage through the cohesin ring https://www.biorxiv.org/content/10.1101/2024.10.30.621121v1

With some help from @raduzabet.bsky.social, I've created a starter pack of researchers working on #Epigenetics, Chromatin and Gene Regulation who are active on here: go.bsky.app/TKp9YAn I've likely missed many people so let me know if you want to be added. Similarly if you would like to be removed.

UPDATE: The NIH just released an icon collection, entirely in public domain and growing: bioart.niaid.nih.gov PS with equipment, I know many look for their PCR cycler or microscopes...

our paper on the ATP-dependent chromatin remodeler SMARCAD1 is published y'all. Cheer for Briana Aboulache and @nhoitsma.bsky.social . This remodeler is regulated by phosphorylation in unexpected ways. 🧪🧪🧪https://www.jbc.org/article/S0021-9258(24)02395-0/fulltext

Happy to announce a new preprint from our lab in collaboration with Richard Hite's lab at Sloan Kettering Institute: www.biorxiv.org/content/10.1... 1/4

Very happy to share the final version of our paper in @NatureGenet, in which we reconstitute chromatin domains and find that nucleosome positioning plays an important role in higher-order folding of chromatin: www.nature.com/articles/s41...