In the Ring: Details of the bacterial cell division structure the Z-ring revealed using cryo-ET 📷 Adrián Merino-Salomón et al @mpibiochem.bsky.social in @elife.bsky.social ➡️ bpod.org.uk/archive/2025... with John Ankers

I am so grateful Apple brought Preview to the iPad on OS 26. It has made being without my MacBook for the week so much easier. The iPad is still not a laptop replacement, but it has gotten much closer to that recently.

Excited to share our preprint on cytoskeletal organization in astrocytes! biorxiv.org/content/10.110… Postdoc @mewynne759 looked at microtubules, IFs/GFAP & actin along long elaborate processes. Wonderful to collaborate w postdoc @dharshinigopal & @NogalesLab on cryo-ET 🤓 1/x

Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️ www.biorxiv.org/content/10.1... #teamtomo

Meanwhile in “war ravaged Portland.” #PDX

Woo! My lab's Nature Protocols paper is out today. In a nutshell, the protocol shows how to use our Cryo-ET software to solve high-res protein structures. I don't think any of the other authors are here, but congrats to Hsuan-Fu, Ye, and Wendy for all of their work! www.nature.com/articles/s41...

Structural dynamics of mitochondrial ATP synthase in Chlamydomonas reinhardtii revealed by in situ CryoET https://www.biorxiv.org/content/10.1101/2025.09.10.674987v1

I realize I don't post much, but figured I would share an update. As of today, I am officially supported by the Interdisciplinary Training in Microbial Pathogenesis and Immunology T32 Training grant.

"We couldn’t be more excited about the transformational potential of this work for humanity." - Phil and Penny Knight on their historic $2 billion gift to @ohsuknight.bsky.social to transform the future of cancer care and set a new standard globally. #GiveCancerHell

Took this photo of the Tilikum Bridge on my walk this morning. Beautiful weather this morning. #PDX

Gokul Adethya, Bhanu Pratyush Mantha, Tianyang Wang, Xingjian Li, Min Xu: SaSi: A Self-augmented and Self-interpreted Deep Learning Approach for Few-shot Cryo-ET Particle Detection https://arxiv.org/abs/2505.19948 https://arxiv.org/pdf/2505.19948 https://arxiv.org/html/2505.19948

This is really nice CryoET work and video. paper link: www.science.org/doi/10.1126/...

‘Forever chemicals’ self-assemble into stable structures resembling biological cells — a behaviour that could help to explain why they stick around for decades in the soil or groundwater https://go.nature.com/3F02sEd

I talked to @kakape.bsky.social about this for @science.org, along with many other colleagues including those with different views on regulatory oversight. We all agree: it’s too vague to be implemented, effective, or enforceable. www.science.org/content/arti...

When the White House fact sheet says manipulates "viruses and other biological agents," it reads as potentially restricting a lot more than research on viruses of pandemic potential. I am concerned in terms of how this will ultimately be interpreted into policy.

“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩‍💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

Our latest paper is out! Congratulations to @leaswistak.bsky.social et al for this amazing #cryoET work that elucidates the initial stages of #T3SS dependent vacuolar damage during #Shigella infection. We will do a deep-dive later! journals.plos.org/plosbiology/...

🚨New preprint!🚨 #teamtomo We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles. Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science

We have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples! Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵 #CryoET #TeamTomo www.biorxiv.org/content/10.1...

Join us April 8th for a software webinar with @alisterburt.bsky.social from @genentech.bsky.social sharing the latest on Warp. Warp Alister Burt, Ph.D., Computational Scientist, Genentech Tuesday, April 8, 2025 - 12:00pm ET Register here: buff.ly/mXUYR4A #SBGrid #Webinars #StructuralBiology

You always know the inevitable is going to happen sooner or later and you think you’re prepared, but you never are. Today my best friend of 20 years crossed the rainbow bridge. I’ll always treasure the memories we shared together. Rest well, Coco. 2004-2025

Folks, I can’t stress this enough: Lock your credit! Lock your credit! Lock your credit! Lock your credit! Lock your credit! Lock your credit! Lock your credit! Lock your credit! Lock your credit! Lock your credit! Lock your credit! Lock your credit! Lock your credit! Lock your credit!

Honored to be part of this amazing work, where synaptic vesicle fusion has been studied by a clever combination of optogenetic stimulation and cryoET. I welcomed the opportunity to contribute with mesoscopic simulations involving dynamic membranes. Preprint: www.biorxiv.org/content/10.1...

Hello everyone on Bluesky! 🧪 We are EMBO, the organization of more than 2,100 leading researchers that promotes excellence in #lifesciences in Europe and beyond. Read more: www.embo.org/about-embo/m...

Pranav N. M. Shah et al.: TomoCPT: a generalizable model for 3D particle detection and localization in cryo-electron tomograms ##CryoET##ParticlePicking##SubTomogramAveraging@UniofOxford@IEUniversity...##IUCrhttps://journals.iucr.org/paper?S2059798325000865

Hey #teamtomo, if you often find yourself using the excellent membrain-seg from @lorenzlamm.bsky.social et al., you might find my napari plugin "napari-segselect" useful. Let's say your tomogram contains the edges of two bacterial cells, each with a membrane and cell wall:

My proposal for the Pacific Northwest Center for Cryo-EM has been approved! #CryoET #Victory

The Senate Finance Committee is about to vote on RFK Jr’s nomination. I tuned in late & missed the chair’s opening statements but now listening to Wyden talk about how unfit he is. The list is long.

New review on structural glycobiology in glycosciences: "From enzymes to organelles". Carbohydrate processing enzymes, glycosylation networks in vitro, Cryo-ET in direct visualisation of native biology , challenges for studying glycosylation by cryo-ET. tinyurl.com/4jnv85xt #glycotime

It's ironic how Wikipedia of all things, the bane of my secondary school teachers, remains the purest part of the internet these days.

I put together a small guide on how to get started with Fiji. Please distribute to anyone who might benefit form this! zenodo.org/records/1477... #imageanalysis #fiji #imagej #training

My first contributions to the grid graveyard. #Failure

I'd like to draw your attention to this truly excellent paper from the Taraska lab on cryoET of plasma membrane associated proteins. Everyone who is thinking about probes in the cryoET space should also see what they could do with ferritag (fig 6) www.nature.com/articles/s41...

WOW!😍 This is exactly the type of development we hoped the Chlamy dataset would help empower! Lots of organelles and cellular features labeled automatically, context-aware particle picking, & area-selective template matching #TeamTomo 🔬 Big props to @mgflast.bsky.social @thomsharp.bsky.social 🧪🧶🧬

Is the data in the cryoET data portal (built by the CZI) owned by Meta once you upload it there?

I'm pretty sure being riveted by the news is absolutely never a good thing

Wow. This is the first neat paper of 2025! From a baby plate of HEK cells and a uL column

Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET https://www.biorxiv.org/content/10.1101/2025.01.09.632151v1

Our lab's research assistant made extra LB plates, so we did the logical thing and put our fingers on antibiotic-free media to see what would grow. Here are the results. #microbiology

1) Since I am the only Herzik lab member on @bsky.app, I get the privilege to announce our most recent publication from the Herzik/Tezcan labs. I got the wonderful opportunity to contribute on the cryoEM study of the oxygen protection mechansim employed by cells to protect Nitrogenase!

Paper of interest for the #CryoET folks. www.nature.com/articles/s41...

Tomorrow(!) morning at 9AM pacific, I am giving a webinar for EMS discussing the surface morphometrics pipeline and our approach to contextual structural biology #teamtomo. Free to attend, hope to see you all there! us06web.zoom.us/webinar/regi...

I am about to be recruiting two post-docs to work on cryoEM and cryoET of amyloid aggregates... Anyone interested please drop me a line while the wheels of our HR department turn.............

Check out @everylibrary.bsky.social. They help grassroots groups across the country defend and support their local library against book banning, illicit political interference, and threats of closure. They also help libraries advocate for funding.

We strongly suggest that academic publishers and other platforms that host research rapidly implement a Share to Bluesky button for their articles. Here's how: docs.bsky.app/docs/advance... #AcademicSky #HigherEd #Altmetrics

TIL Word has activated --without telling anyone- AI-powered features by default. It's called "Connected Experiences" and you can deactivate it in "Privacy" in a Mac or "Trust Center" in a PC. Absolutely outrageous. What the hell.

Any word on a BlueSky for iPad? There is a lot of wasted space using it on iPadOS Safari. #iPad #BlueSky

Looking for a tag to localise fluorescent proteins to the plasma membrane? Unsure which tag might work in your species? We generated a toolkit of 11 membrane-localising tags, which can be screened rapidly by microinjecting mRNA in your species of interest. 1/4 www.biorxiv.org/content/10.1...