Our Wadjet-II paper by @roisnehamelinf.bsky.social is out in polished form: www.cell.com/structure/fu... With a great preview by @kevincorbett.bsky.social & @amardeeep.bsky.social highlighting the potential of Wadjets: Wadjet—Keeping a watchful eye on circular DNA. www.cell.com/structure/fu...

🚨 2 × PhD positions @EPFL! 🚨 Help us push the boundaries of fluorescence microscopy - DNA nanotech, custom optics & spatial omics in Lausanne 🇨🇭. Start Jan 2026. Send CV + motivation + 2 refs → [email protected] #PhD #Hiring #microscopy #SuperResolution #SpatialOmics #DNAPAINT #FLASHPAINT

Now peer reviewed and in its final form @natcomms.nature.com www.nature.com/articles/s41...

Less than two weeks left to apply! Postdoc positions in my lab to study 1. initiation of DNA replication. 2. chromatin replication/epigenetic inheritance. Great for biochemists, biophysicists & structural biologists. Deadline 3 August 2025. crick.wd3.myworkdayjobs.com/External/job...

Our new non-denaturing nanoscale 3D DNA tracing technology provides single-cell (and sister-chromatid specific!) readout of chromatin structure with 10kb genomic resolution - Check it out! www.nature.com/articles/s41... Congratulations to Kai and Oyvind!!

Out now: Structure of an archaeal ribosome reveals a divergent active site and hibernation factor @jhdcate.bsky.social @banfieldlab.bsky.social @diptinayak.bsky.social & co #microsky www.nature.com/articles/s41...

Very exciting new technology manuscript from @alicepyne.bsky.social lab! High-res AFM + AI pipeline quantifies complex DNA topology. t.co/qxD7o7ASl6 Very happy to contribute with @singlemoleculenev.bsky.social plasmids replicating past barriers in Xenopus egg extract. Congratulation everyone!

#pictureofthemonth: Illustration of the read-write mechanism by which ubiquitin tags are placed on histone H2A along genes to influence their expression. More: www.biochem.mpg.de/painting-gen... July – institute calendar of @mpibiochem.bsky.social and @mpiforbi.bsky.social Image by Tamara Bäßler

Check out this new review (with animations, natch) of mechanisms of licensing origins of DNA replication - a wonderful (and continuing!) collaboration with Bruce Stillman @cshlnews.bsky.social and John Diffley @crick.ac.uk! www.nature.com/articles/s41...

🥳Amazing publication from the Hornung lab, in collab. with the Carell lab, on the innate immune system: how modified RNA tricks the immune system. Big shout-out to the two first authors, Marleen Bérouti and Mirko Wagner! www.cell.com/cell/fulltex... @v-hornung.bsky.social @m-berouti.bsky.social

It also has devastating cuts to virtually everything I want my taxes to support: science, a safety net, conservation, etc. Call your Senator today--30 seconds will work. Tell them to vote NO on this bill. Tillis is at: (202) 224-6342 3/n

The proposed budget slashes NIH by ~40%, devastating medical research, clinical trials, treatment breakthroughs—life-saving work. Thousands of lives at risk for tax cuts for the rich? NO THANKS. Tell your senators to oppose the cuts HERE: zurl.co/qiKdX zurl.co/RCR0y #CutsCostLives

new preprint alert from the lab: bringing some order into the wide and wild diversity of archaeal histones. Who has acidic histones? who has multiple histones? what histone combinations do archaea employ and why? find all of this and more here www.biorxiv.org/content/10.1...

Postdoc positions open in my lab (Francis Crick Institute) to study 1. initiation of DNA replication. 2. chromatin replication/epigenetic inheritance. Great for biochemists, biophysicists and cryo-EM/cryo-tomography scientists. Deadline 3 August 2025. crick.wd3.myworkdayjobs.com/External/job...

This is a piece that I and @karsten-rippe.bsky.social discussing a lot, and a topic that is very close to my heart. The editors @naturerevgenet.bsky.social gave us the stage to do so, and the final version of our review is now available under this link: rdcu.be/erP1u A short thread follows 1/n

Latest #CDlab paper online now: Cohesin supercoils DNA during loop extrusion www.cell.com/cell-reports... This extensive study was led by Jan Michael Peters at Vienna; our lab contributed mostly modeling of plasmid supercoiling (@romanbarth.bsky.social‬) and single-molecule data (Richard Janissen).

We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social! www.biorxiv.org/content/10.1... #Chromatin #Asgard #Archaea #cryoEM

Our new study of chromatin ubiquitylation by variant PRC1 on the single-molecule scale: We visualize directly how vPRC1 ubiquitylates neighboring nucleosomes during a single binding event, showing a potential mechanism how H2Aub domains are established. www.science.org/doi/10.1126/...

Excited to share our new preprint on HLp—a bacterial histone from Leptospira perolatii that forms stable tetramers and wraps ~60 bp of DNA: "DNA Wrapping by a Tetrameric Bacterial Histone" www.biorxiv.org/content/10.1... @mpi-bio-fml.bsky.social

Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩 We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨

📢Stigler lab in collaboration with the Holt lab decipher that LINE-1 ORF1p forms super-saturated co-condensates with RNA that enable DNA binding and nuclear entry during mitosis. Congratulations!👏👏👏 Details: www.science.org/doi/10.1126/...

Want to learn more about chromatin and its dynamics? Read this review by Charlotte and Jolijn in our lab, aimed at giving an overview on this and the ways this is dealt with during transcription, replication and damage repair. Thanks to Biochemistry for inviting us! doi.org/10.1021/acs....

3rd edition of the fantastic UK DNA replication meeting is happening next September in Edinburgh. Don't miss out 🧬🏴󠁧󠁢󠁳󠁣󠁴󠁿 www.biochemistry.org/events-and-t...

Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social, @marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social 🔗 Preprint here tinyurl.com/3a74uanv

🚨New preprint🚨We developed a transverse flow imaging approach to directly visualize bacterial replisomes challenged by topological barriers and discovered a fast rotational mode of replisome operation. Fantastic work by thomasmretzer.bsky.social and Hamdan Lab! tinyurl.com/replisomerot...

🔬 New from the Farnung Lab: We established a fully in vitro reconstituted chromatin replication system and report the first cryo-EM snapshots of the human replisome engaging nucleosomes. Brilliant work by @felixsteinruecke.bsky.social with support from @jonmarkert.bsky.social! tinyurl.com/replisome

I ❤️ this new paper on RAD52 at replication forks, just published @nature.com. Cryo-EM reveals a unique double-ring structure that blocks reversal of replication forks. www.nature.com/articles/s41...

How do SMC complexes capture DNA? 🧪 Led by Frank Bürmann, Jan Löwe’s & Mark Dillingham’s groups have identified the directional loading mechanism the bacterial SMC complex MukBEF uses to capture & ingest DNA, & found a phage inhibitor to this pathway. Read more: tinyurl.com/4s8nrrkk #LMBResearch

Our Lise Meitner Research Group Leader Maria Sokolova receives this year's Heinz Maier-Leibnitz Prize 2025 for her outstanding research on bacteriophages. Congratulations!🥳 ❕ www.dfg.de/de/service/p... #Award, #WomenInScience, #bacteriophages @arimiora.bsky.social @maxplanck.de

🚨New paper🚨Our new study with the lab of Phil Cole uncovers how SIRT6 dynamically adapts structurally and enzymatically to target diverse histone acylations (e.g., H3K27ac) within nucleosomes. @jonmarkert.bsky.social doi.org/10.1016/j.jb... #Chromatin #SIRT6 #CryoEM

🤩Save the date: Marieke Oudelaar will speak on Molecular Drivers of 3D Genome Folding and Their Function in Gene Regulation💬 We look forward to meeting @mariekeoudelaar.bsky.social😆 Join us on April 8 at 1:30 pm🤗 ➡️Lynen Lecture Hall @mpi-nat.bsky.social

Check out our review on DNA end resection! With Raphael Ceccaldi, www.nature.com/articles/s41...

Happy to announce that our new work on studying how gliding diatoms modulate the curvature of their paths is now on #bioRxiv www.biorxiv.org/content/10.1.... Great work in collaboration with @nic-poulsen.bsky.social on a nucleation grant by @poldresden.bsky.social #OpenScience #Preprints #diatom

Labeling your axes and then explaining them slowly is perhaps the biggest bang for the buck you can get for making your slides more understandable.

As some of you already know, I was appointed Dean of Science at Columbia in December and began my role on Jan 1. It’s an honor to support our students, postdocs, and faculty—especially in this challenging moment. Science at Columbia is strong. #ColumbiaScience fas.columbia.edu/news/ruben-g...

I wrote an opinion piece for my hometown South Carolina newspaper that was published today. Support science funding for our future! Standing up for science today and always! tinyurl.com/mr3vuphr

How do replisomes walk on DNA? And what happens when they run into a G-quadruplex? @sahilbatra.bsky.social and @benallwein.bsky.social provide unexpected insight in our latest paper with Richard Hite @mskcancercenter.bsky.social @science.org www.science.org/doi/10.1126/.... Congrats to all authors!

𝐍𝐞𝐰 𝐦𝐞𝐜𝐡𝐚𝐧𝐢𝐬𝐭𝐢𝐜 𝐢𝐧𝐬𝐢𝐠𝐡𝐭𝐬 𝐢𝐧𝐭𝐨 𝐭𝐡𝐞 𝐫𝐞𝐠𝐮𝐥𝐚𝐭𝐢𝐨𝐧 𝐨𝐟 𝐡𝐢𝐬𝐭𝐨𝐧𝐞 𝐬𝐮𝐩𝐩𝐥𝐲! Excited to share our latest collaboration led by tkjeong.bsky.social and ciaranfrater.bsky.social! Structural determination of the histone chaperone ASF1 and its regulator CODANIN-1 uncovers how CODANIN-1 counters ASF1 activity. 🧵👇

#pictureofthemonth: Illustration of combined histone proteins (center) so a DNA thread (foreground) can wind around them like a spool. www.biochem.mpg.de/duderstadt March – institute calendar of @mpibiochem.bsky.social and @mpiforbi.bsky.social Image by Karl Duderstadt @duderstadtlab.bsky.social

A new PhD position is available in my lab to explore DNA replication mechanisms using single-molecule approaches. Apply by 19th of March: www.crick.ac.uk/careers-stud...

Amazing news on thr #Fiji front. The new launcher holds beautiful promises for better bioimage analysis tools, and not only for Fiji!! See this summary thread by Curtis forum.image.sc/t/fiji-2024-...

SAVE THE DATE! The next EMBO | EMBL Symposium on 'DNA replication and genome maintenance: from basic biology to disease' will be held October 20-23, 2026.   Organized by Helle Ulrich (IMB), Johannes Walter (Harvard) and Anja Groth (Danish Cancer Institute). www.embl.org/about/info/c...

Proud to share our latest publication in @narjournal.bsky.social! A fantastic team effort led by @marcelwerner.bsky.social and Manuel T., where we explore the chromatin dynamics at transcription-replication conflicts (TRC) sites and their impact on genome stability using a novel TRC reporter system.

𝐇𝐨𝐰 𝐝𝐨 𝐜𝐞𝐥𝐥𝐬 𝐫𝐞𝐦𝐞𝐦𝐛𝐞𝐫 𝐰𝐡𝐨 𝐭𝐡𝐞𝐲 𝐚𝐫𝐞 𝐚𝐟𝐭𝐞𝐫 𝐃𝐍𝐀 𝐫𝐞𝐩𝐥𝐢𝐜𝐚𝐭𝐢𝐨𝐧? Our new study “Disabling leading and lagging strand histone transmission results in parental histones loss and reduced cell plasticity and viability” is out in 𝘚𝘤𝘪𝘦𝘯𝘤𝘦 𝘈𝘥𝘷𝘢𝘯𝘤𝘦𝘴. Led by @lleonie.bsky.social @biranalva.bsky.social 🧵 More below👇

Meet Carina Baer de Oliveira Mann, our newly appointed professor of #Biomolecular Cryo-Electron Microscopy, in the latest NewIn episode. She aims to identify #molecularswitches in the human #immunesystem that activate the immune defenses: go.tum.de/157504 @oliveiramann.bsky.social 🎥ProLehre

The UK&Ireland single-molecule meeting 2025 will be held in Oxford on May 14 and 15 - deadline for abstract submission March 7 - submit one and join us! Here is the link: lumicks.com/uk-single-mo...

🌟 <interact> 2025 Registration Now Open! 🌟 Prepare to meet our fantastic keynote speakers while enjoying inspiring networking with the participants 🧬✨ Explore and celebrate the impact of our research on the world around us alongside like-minded scientists. Let the <interact>ion begin!!

Join our online information session re. our 2025 round for Fellows at Oxford Biological Physics (original ad below)! Date: Jan 30, 2025 Time: 11 am -12 noon UK time Location: Online via Zoom (rsvp by Mon 27/1/2025 at [email protected]) Oxford Biological Physics Faculty

Opening for a senior postdoc in our single-molecule DNA replication lab! Deadline January 15, 2025!

Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @jonmarkert.bsky.social! tinyurl.com/setd2