Chris Caffalette
@caffalette.bsky.social
📤 256
📥 600
📝 31
Postdoc at The Rockefeller University
reposted by
Chris Caffalette
Helena Watson
29 days ago
ps. Interested in alternative ways to use LM data? Check out preprints from: Vojta Pražák + colleagues:
www.biorxiv.org/content/10.6..
. Giulia Zanetti’s group:
www.biorxiv.org/content/10.1..
. Both show how LM tomos can guide HM acquisition when events of interest are hard to spot from search maps
0
5
3
reposted by
Chris Caffalette
Helena Watson
29 days ago
Huge thanks to Michael Grange (
@michaelgrange.bsky.social
), Fabian Eisenstein (
@eisfabian.bsky.social
) , and Victoria Garcia-Giner — really grateful for all their help and support on this work!
1
6
2
reposted by
Chris Caffalette
Helena Watson
29 days ago
Would love everyone to give it a go! Cryo-ET is already a great tool to study structures in context — interleaved multi-magnification acquisition captures even more of that context, without compromising throughput or STA resolution Excited to see what people do with BIGSMALL! (6/6)
1
9
2
reposted by
Chris Caffalette
Helena Watson
29 days ago
BIGSMALL is now implemented in SPACEtomo. Setup is easy: - define low mag parameters with SerialEM Search preset - add LM + HM targets in the same beam-shift group - acquire! (5/6)
2
21
4
reposted by
Chris Caffalette
Helena Watson
29 days ago
BIGSMALL interleaving means there are minimal downsides: ✔️ Maintains throughput (no added stage movements) ✔️ Preserves high-resolution data We reach 3.7 Å for the 𝘌. 𝘤𝘰𝘭𝘪 50S subunit in both interleaved and conventional acquisitions. (4/6)
1
6
1
reposted by
Chris Caffalette
Helena Watson
29 days ago
Cryo-ET isn’t just about high-resolution structures. Low-mag data lets you analyse cell biology across much larger spatial scales. We show this by mapping fenestrations in the RER across extended regions in 𝘊. 𝘦𝘭𝘦𝘨𝘢𝘯𝘴. (3/6)
1
10
1
reposted by
Chris Caffalette
Helena Watson
29 days ago
BIGSMALL captures both high-resolution structures and large-scale cellular context at the same time, by acquiring high- and low-magnification images at every tilt in a tilt-series. This gives: 🟩 large FOV, low mag tomos 🟥 high res, high mag tomos from the same sample region (2/6)
1
7
2
reposted by
Chris Caffalette
Helena Watson
29 days ago
Preprint 🧵! I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition! BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a
www.biorxiv.org/content/10.6...
(1/6)
5
211
72
reposted by
Chris Caffalette
Savage-Dunn lab
12 months ago
CUNY and SUNY colleagues: ICYMI Please sign and share this letter to the NY Congressional Delegation asking them to protect NIH and NSF funding!
docs.google.com/forms/d/e/1F...
add a skeleton here at some point
0
2
1
reposted by
Chris Caffalette
Ed Boyden
12 months ago
MIT News reports on focused research organizations (FROs), non-profit startups, developed by some of our group members and alumni. FROs can work on problems that are a poor fit for academia or the for-profit startup world.
news.mit.edu/2025/former-...
loading . . .
Former MIT researchers advance a new model for innovation
Developed by former MIT researchers, focused research organizations (FROs) undertake large research efforts and have begun to contribute to scientific advances.
https://news.mit.edu/2025/former-mit-researchers-advance-new-model-innovation-0606?fbclid=IwZXh0bgNhZW0CMTEAAR4JdojLx13CF3wDcpSdGX6-pxp4nJc7nsISXDYGOuWGRPIvmrd_7XyO-umYTA_aem_NgF6XNIt8XEIIzDz0529ug
0
20
10
reposted by
Chris Caffalette
Sjors Scheres
12 months ago
The amazing Sofia Lövestam initiated the below project, when she became interested in the vault particles that we sometimes observe in
#cryoEM
images of brain-derived
#amyloid
filaments.
www.biorxiv.org/content/10.1...
loading . . .
Cryo-EM structure of the vault from human brain reveals symmetry mismatch at its caps
The vault protein is expressed in most eukaryotic cells, where it is assembled on polyribosomes into large hollow barrel-shaped complexes. Despite its widespread and abundant presence in cells, the bi...
https://www.biorxiv.org/content/10.1101/2025.05.27.656403v1
2
60
23
reposted by
Chris Caffalette
Rock Lab
about 1 year ago
This isn’t just numbers on a table–it’s my lab and the labs of my colleagues. Terminated grants mean shuttered projects, lost talent, and stalled progress against deadly pathogens. The damage to science–and to public health–is real and immediate. Thanks
@peterhotezmdphd.bsky.social
for speaking out.
add a skeleton here at some point
0
3
4
reposted by
Chris Caffalette
Falk Schneider
about 1 year ago
Back to
@mblscience.bsky.social
Woods Hole - teaching on the Pair Up Microscopy Course. Great experience and awesome participants. Wild - last time here I was a student (Physical Biology of the Cell Course in 2018). Now introducing my lab. Thanks Abhishek Kumar for organising and the invitation.
1
29
7
reposted by
Chris Caffalette
Carolyn Bertozzi
about 1 year ago
If people of means are interested in establishing a 2025 “Institute for Advanced Study” for displaced/defunded biomedical scientists, I know many people who will help, including myself.
@mcuban.bsky.social
@arcadiascience.com
@arcinstitute.org
add a skeleton here at some point
4
116
38
reposted by
Chris Caffalette
Michael Baym
about 1 year ago
Just found out my two related NSF grants (on discovery of phages that target antibiotic resistance vectors and building synthetic systems to understand the biology of those vectors) were also caught in Harvard’s mass grant termination
10
64
21
reposted by
Chris Caffalette
Daniel Kronauer
about 1 year ago
Here’s another spectacle of New York spring entomology: Eastern tent caterpillars (Malacosoma americanum) are among the most social lepidopterans. In May, you can find their silken tents, in which they aggregate (and defecate).
1
18
2
reposted by
Chris Caffalette
Daniel Kronauer
about 1 year ago
They have three daily feeding bouts, during which they exit the nest and move together to distant feeding sites. They mark their route with silk and trail pheromone. Successful “scouts” can even recruit the colony to new food sources. They’re kind of the ants among the butterflies. Woodstock, NY.
0
11
1
reposted by
Chris Caffalette
Whitney Gibbs
about 1 year ago
My lab at NYU is hiring a postdoc interested in RNA biology and local translation in neurons following axonal injury/neurodegenerative diseases!! Apply here:
apply.interfolio.com/165681
loading . . .
Apply - Interfolio {{$ctrl.$state.data.pageTitle}} - Apply - Interfolio
https://apply.interfolio.com/165681
0
38
36
reposted by
Chris Caffalette
Shira Weingarten-Gabbay
about 1 year ago
Tomorrow at the Systems Virology Journal Club,
@tamanash.bsky.social
will present his beautiful work with
@harmitmalik.bsky.social
on optimizing temperature-dependent trade-off between protein production and processing in alphaviruses through stop codon selectivity
pubmed.ncbi.nlm.nih.gov/40249804/
add a skeleton here at some point
0
8
4
reposted by
Chris Caffalette
Jeremy Baskin
about 1 year ago
Groundbreaking study in Cell from
@leventallab.bsky.social
: phospholipid asymmetry is a defining feature of the plasma membrane and cholesterol fills the holes — major implications for how this membrane works. A
#lipidtime
must-read!
www.cell.com/cell/abstrac...
loading . . .
Cell membranes sustain phospholipid imbalance via cholesterol asymmetry
This work challenges a major assumption in cell biology by showing that lipid bilayers can have drastically different phospholipid abundances between their two leaflets. This lipid abundance asymmetry...
https://www.cell.com/cell/abstract/S0092-8674(25)00270-3
2
201
80
reposted by
Chris Caffalette
Victor Ambros
about 1 year ago
Rachael, thank you for this. We can all benefit from the example of your courage and wisdom in speaking out.
add a skeleton here at some point
1
6
2
reposted by
Chris Caffalette
Journal of Cell Biology
about 1 year ago
Structural insights into the coupling between VCP, an essential unfoldase, and a deubiquitinase. New study from Lauren Vostal, Tarun Kapoor and colleagues
@rockefelleruniv.bsky.social
:
rupress.org/jcb/article/...
#StructuralBiology
#Biochemistry
#ProteinHomeostasis
#CryoEM
0
11
3
reposted by
Chris Caffalette
Ricardo D. Righetto
about 1 year ago
WE GOT THE COVER!!!
add a skeleton here at some point
5
60
9
reposted by
Chris Caffalette
Florent Waltz
about 1 year ago
The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in
@science.org
🎉 You can find the full story here
www.science.org/doi/10.1126/...
@verenaresch.bsky.social
did a wonderful job on the cover and animation, she really brought it to life 👩🎨
add a skeleton here at some point
12
279
94
reposted by
Chris Caffalette
Philipp Brand
about 1 year ago
Congratulations to first authors
@rufeili.bsky.social
and
@tshindmarsh.bsky.social
!!!!
0
1
1
reposted by
Chris Caffalette
Philipp Brand
about 1 year ago
Here is the paper, worth a read for those interested in behavior, neuroscience, and mating competition:
www.cell.com/cell/fulltex...
loading . . .
https://www.cell.com/cell/fulltext/S0092-8674(25)00037-6
1
4
2
reposted by
Chris Caffalette
Philipp Brand
about 1 year ago
Proud that one of my photographs has made the cover of Cell. (first 'official' photograph publication) Very happy to contribute the pic and highlight the latest publication of the Ruta lab.
8
43
5
reposted by
Chris Caffalette
Hironori Funabiki
about 1 year ago
In preparation for the lecture tomorrow, I opened "The Cell in Development and Heredity" by E.B. Wilson (Columbia Univ), and realized that this 3rd edition was published in 100 years ago. So many things happened since then, including the fact that I, a Japanese Prof in NY, own and read the book.
1
6
1
reposted by
Chris Caffalette
Ben Engel
about 1 year ago
Fantastic
#TeamTomo
story on retrotransposon particles by
@svenklumpe.bsky.social
and friends. Take a look! 🧪🧶🧬
add a skeleton here at some point
0
20
3
reposted by
Chris Caffalette
Pauli Group (posts by Andi Pauli)
about 1 year ago
Retrotransposon-derived capsids in the Dros. ovary revealed by CryoET! Amazing PhD work by
@svenklumpe.bsky.social
(still MPI Biochemistry) in collab with
@juliusbrennecke.bsky.social
lab - congrats to all authors! Really looking forward to exciting times with Sven
@viennabiocenter.bsky.social
!
add a skeleton here at some point
0
16
4
reposted by
Chris Caffalette
Ricardo D. Righetto
about 1 year ago
This has to be one of the coolest papers of the year: retrotransposon VLPs, visualized inside cells!
#TeamTomo
#CryoET
add a skeleton here at some point
1
14
1
reposted by
Chris Caffalette
Lander Lab
about 1 year ago
Congrats to our postdoc Jan-Hannes Schaefer
@jhschaef.bsky.social
on being awarded a fellowship from the Walter Benjamin Program! Jan is collaborating with the Kelly lab here at Scripps Research
@scripps.edu
to understand how transthyretin forms amyloids that lead to heart disease.
0
31
6
reposted by
Chris Caffalette
Tim Stearns
about 1 year ago
Good luck with your defense! We’re all worried about the near-term, but the long-term will favor those like yourself who want to do excellent science on important problems. You are the future of science, and we have to do everything we can to ensure that there are great opportunities for you.
2
9
1
reposted by
Chris Caffalette
Luke Lavis
about 1 year ago
Hey
#chemsky
and beyond, with all that is going on, I am curious what expensive, high margin molecules we could provide to the community for free. What things do you use all the time, cost way too much, and would make a difference? Alexa Fluor 488 NHS? Other dyes? Other molecule?
7
68
31
reposted by
Chris Caffalette
Zunlong Ke
about 1 year ago
Beautiful story on HIV-1 Matrix maturation and seeing it out in complete story by my postdoc colleagues James and Dominik from the Briggs group
@briggsgroup.bsky.social
Congratulations to all!
add a skeleton here at some point
0
10
1
reposted by
Chris Caffalette
Max Wilkinson
about 1 year ago
we recently found some really neat RNA-guided DNA-cutting systems in phages. Despite remarkable similarities to CRISPR systems, including encoding guide RNAs in arrays, they appear entirely evolutionarily distinct (but definitely related to snoRNAs 🤓) We decided to call them TIGR-Tas systems 🐯
loading . . .
2
209
81
reposted by
Chris Caffalette
Carolyn Ibberson
over 1 year ago
I created a brief spreadsheet of reductions I've heard of so far. Any additions you know of (especially if you have the links/receipts) would be great:
docs.google.com/spreadsheets...
loading . . .
Graduate Reductions Across Biomedical Sciences (2025)
https://docs.google.com/spreadsheets/d/1FLlILMkKFHgKwUawLqdwW2o84CLfBARQux5n-Pnbc40/edit?usp=sharing
38
461
386
reposted by
Chris Caffalette
Jeremy Berg
over 1 year ago
Bluetorial: The path to a new pain medication with little potential for addiction Perhaps a good story to share with folks who want to learn about how biomedical research works and where advances come from.
1
182
101
reposted by
Chris Caffalette
over 1 year ago
Thanks to everyone for coming out in solidarity today in New Haven despite the chilly temperatures. Props to the speakers for sharing their stories and experiences. Onward!
@valeriejhorsley.bsky.social
@aaup.bsky.social
#labor4highered
0
16
3
reposted by
Chris Caffalette
Tim Stearns
over 1 year ago
The Democratic staff of the Committee on Science, Space, and Technology is surveying the impact of the mass firings of federal employees from science agencies.
democrats-science.house.gov/sciencefirings
loading . . .
Were you fired by President Trump? | House Committee on Science, Space and Technology
The House Committee on Science, Space and Technology
https://democrats-science.house.gov/sciencefirings
0
5
4
reposted by
Chris Caffalette
Valerie Horsley
over 1 year ago
We had a great
@aaup.bsky.social
HANDS OFF rally today in New Haven CT! Thx to coorganizer
@luskinglab.bsky.social
and all of our speakers! When we fight, we win!
0
6
2
reposted by
Chris Caffalette
Jeremy Berg
over 1 year ago
Thanks. You folks are the primary things that keeps me motivated to fight as hard as I effing can. This system greatly enriched my life and my ability to contribute, and others deserve the same opportunities.
add a skeleton here at some point
2
136
7
reposted by
Chris Caffalette
over 1 year ago
Good news! The website for the Yale Chapter of
@aaup.bsky.social
just dropped:
aaupyale.org
Just in time to help make a difference. ❤️🩹
loading . . .
AAUP Yale
https://aaupyale.org/
0
11
5
reposted by
Chris Caffalette
Alexis Rohou
over 1 year ago
Cultural revolution in the US. Elevation of ignorance and empowerment of the ignoramus. Large scale, capricious upheaval of core government functions. This is likely to lead to illness, hunger and poverty for many.
add a skeleton here at some point
0
4
2
reposted by
Chris Caffalette
Alushin Lab
over 1 year ago
My heart goes out to all my NIH people. Unbelievable.
0
29
5
reposted by
Chris Caffalette
Tom Hindmarsh Sten
over 1 year ago
Excited see the final portion of my PhD work with Vanessa Ruta out today in
@cellpress.bsky.social
, in the midst of everything. In time for valentines day,
@rufeili.bsky.social
and I describe how competition between males sculpt mating decisions in 🪰🫶.
www.cell.com/cell/fulltex...
loading . . .
Male-male interactions shape mate selection in Drosophila
Male-male competition strongly influences mate selection in Drosophila, with males performing wing flicks to repel their rivals and jam a female’s perception of other potential mates. Recruitment of p...
https://www.cell.com/cell/fulltext/S0092-8674(25)00037-6
5
39
15
So exciting! It makes me so happy to see a round of private funding toward preserving the magical place that MBL is!
add a skeleton here at some point
over 1 year ago
0
3
0
reposted by
Chris Caffalette
Philipp Brand
over 1 year ago
I'm thrilled to be opening my own lab this Fall at the
@maxplanck.de
MPI for Brain Research in Frankfurt. The lab is going to investigate the neuronal and genetic underpinnings of behavioral evolution. We are hiring on all levels. Don't hesitate to share widely and reach out if interested.
21
141
52
reposted by
Chris Caffalette
Ben Engel
over 1 year ago
Thanks for highlighting the depositions
@uermel.bsky.social
! The CZ
#CryoET
data portal and EMPIAR are incredibly important community resources for sharing tomo data, helping accelerate method development and biological discovery🧪🧶🧬
0
6
2
Load more
feeds!
log in