Maximising imaging volumes of expanded tissues for inverted fluorescence microscopy https://www.biorxiv.org/content/10.1101/2025.09.12.675899v1

✍️ The Dumont Lab published a new article with contributions from the @minclab.bsky.social Lab 📖 @jcb.org 📃 Mechanical coordination between anaphase A and B drives asymmetric chromosome segregation 🔗 rupress.org/jcb/article-...

5-color STORM with a single laser using cos/sin filters and phasor plots: www.biorxiv.org/content/10.1... We discussed along those lines with @malacridalab.bsky.social in Montevideo last year, great to see that it works!

Mohammad Amin Eskandari, Timo Betz & colleagues @uni-goettingen.de review the current knowledge on intracellular active forces & viscoelastic properties, which are key to understanding how cells can maintain their intracellular organisation and homeostasis. journals.biologists.com/jcs/article/...

Second October issue is online! www.embopress.org/toc/14693178... Cover features work by Rajarshi Chakrabarti and co-workers from Thomas Jefferson University showing that peri-mitochondrial #actin inhibits #Parkin assembly by disrupting ER-mitochondria contacts. www.embopress.org/doi/full/10....

🤔 #mDia also associates with #microtubules.... (Gundersen team in NatCellBio 2001). @microtubule.bsky.social & I pondered over the #FocalAdhesion splitting & merging phenotype we saw with CLASP-depletion for ages. Great to see someone worked out how to quantitate it! www.science.org/doi/10.1126/...

Perspective from Richard McIntosh describing the history of research on #microtubule polymerization in terms of the ideas, technologies, and observations that have emerged as countless researchers have studied the dynamics of these essential cytoskeletal polymers. rupress.org/jcb/article/...

1/ 🎉Excited to share our new preprint @vignjeviclab.bsky.social @davidbrueckner.bsky.social : "Self-organization of tumor heterogeneity and plasticity" biorxiv.org/content/10.1... Tumor heterogeneity and plasticity drive metastasis and relapse. How is tumor patterning coordinated? A thread👇

Just out in @science.org: Together with the lab of @johanneszuber.bsky.social, we used sequential in-vitro/in-vivo screens to decipher metabolic adaptations in tumors. We find that acidosis is a dominant factor that shapes energy metabolism and stress resilience. www.science.org/doi/10.1126/...

First, check out my recent bioRxiv preprint w/ @oweinerlab.bsky.social: www.biorxiv.org/content/10.1... We find contractility shifts the proportion of implantation-competent embryos from young and aged females. Keep reading this for more info!

Monoclonal gammopathy of undetermined significance is a common, asymptomatic plasma-cell disorder in older adults that can progress to cancer or systemic illness. Diagnosis warrants follow-up, but treatment is not required unless progression is confirmed. Learn more: nej.md/473uOsj

🚨 New Preprint! Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons. ▶️ www.biorxiv.org/content/10.1...

A novel immunotherapy strategy using in vivo generation of engineered CAR T cells can reduce fibrosis and restore cardiac function in a mouse model of heart failure, a 2022 Science study reports. Learn more on #WorldHeartDay: https://scim.ag/3IiFJ8h

Can pressure gradients persist over long timescales in animal cells? We induced intracellular pressure gradients and examined the resulting flows in single cells. We reveal surprisingly long lasting pressure gradients. More here: elifesciences.org/reviewed-pre...

SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N

Are 90% of cancer-related deaths due to metastasis? Apparently the overuse of this percentage in reviews has created confusion and questioning of whether this is true. The answer, which is as always cancer type dependent, may be in these references that also people claim they cannot find.

We’re organizing a conference at @ijmonod.bsky.social in Paris, January 2026, in memory of M.P. Sheetz — pioneer in cell mechanics and mechanobiology, co-discoverer of kinesin, and founder of @mbisg.bsky.social. Join us to explore his legacy. mikeinmemoriam.sciencesconf.org

How do #stemcells integrate information to coordinate fate decisions? Delighted to finally see our work showing how growth factors regulate the mechano-osmotic state of the #nucleus and #chromatin to control #pluripotency exit out! www.nature.com/articles/s41... see 🧵 👇

How do cells navigate up gradients of adhesive proteins? 🤔 Termed "Haptotaxis", this effect is ubiquitous in cell migration, but it's mechanism was poorly understood We show that passive friction directs cells & explains complex trajectories on gradients 👉 www.nature.com/articles/s41...

Which is better? Lactated Ringer’s solution or normal saline? The latest video in our partnership with @glaucomflecken.bsky.social summarizes the FLUID trial. 👉 nej.md/DrG26 #MedSky #MedEd #Surgery

Up to 9-channel (3x3 colors) imaging using engineering fluorescent proteins (mTurquoise, mNeonGreen, mScarlet) with distinct lifetimes 🌈😎 🧪 www.cell.com/cell/abstrac...


1/6 Extremely happy to share my PhD work is out on bioRxiv! We discovered that microtubules deform mitotic centrosomes. When we removed them, the SPD-5 scaffold became denser but didn't lose material. This suggests it's an elastically deformable solid! This only happens in mitosis.

In patients with refractory systemic lupus erythematosus, in vivo generation of CD19 CAR T cells by means of lipid nanoparticles led to B-cell depletion, reduced disease activity, and no major toxic effects. Full study results: nej.md/42u4eWF

Decoding #immunometabolism with next-generation tools: Ping-Chih Ho and colleagues review key challenges in exploring metabolic regulation of dendritic cells and T-cells, highlighting emerging methods and strategies for overcoming them www.embopress.org/doi/full/10....

MAPLE-HCM trial: In patients with symptomatic obstructive hypertrophic cardiomyopathy, whether aficamten monotherapy provides greater clinical benefit than monotherapy with the beta-blocker metoprolol is unknown. Research findings are summarized in a new Quick Take video. nej.md/4mWDx5n

Happy to share our new study @ijmonod.bsky.social and @mpi-scienceoflight.bsky.social with C. Duclut and J. Prost. on forces generated by active fluid transport in epithelia and their role in tissue dynamics. Congrats to Huiqiong Wu, G. Arkowitz and R. Chilupuri! www.biorxiv.org/content/10.1...

BETAMI–DANBLOCK trial: Beta-blockers are recommended for myocardial infarction and reduced left ventricular ejection fraction, but potential benefits for preserved or mildly reduced ejection fraction are unclear. Research findings are summarized in a new Quick Take video. nej.md/41DqahQ

✍️ The @minclab.bsky.social contributed to the publication of a new article 📖 @biophysj.bsky.social 📃 Inferring diffusion, reaction, and exchange parameters from imperfect FRAP 🔗 www.cell.com/biophysj/abs...

#Cancer patients display an elevated burden of #CardiovascularDisease. This review from Ambreen, @adrover.bsky.social et al. explores cancer-elicited changes to #neutrophil behaviour and proposes ways in which these may contribute to cardiovascular pathology. rupress.org/jem/article/...

not a joke: Could someone please send me by DM the PDF of my paper which is behind paywall and I don't have access to it... rupress.org/jcb/article-... Thanks !

Intramuscular adipose tissue restricts functional muscle recovery

In a new Perspective article, Josh Morgan discusses how progress in cell biology is hindered by significance testing and the need for a shift to effect size estimation. rupress.org/jcb/article/... #Technology #Reproducibility #CellCycle #CellDivision #Statistics

Technical Review: Atomistic computing of the solid–fluid surface free energy and tension, by Aziz Ghoufi ⚛️ www.nature.com/articles/s42...

What determines the force required to initiate drop sliding? How does drop friction depend on drop velocity? What processes lead to energy dissipation once a drop has started to slide, and how much do they contribute? Hans-Jürgen Butt & co discuss drop friction ⚛️ www.nature.com/articles/s42...

Aceitón, Riobó, Del Valle Batalla, Yuseff et al show that B cells respond to mechanical cues at the immune synapse through ATAT1-dependent microtubule acetylation to coordinate cytoskeletal dynamics & lysosome positioning, enhancing antigen extraction and presentation rupress.org/jcb/article/...

Yadav, Jadhav, @hanasedlackova.bsky.social et al present a novel cell-permeable biotin-HaloTag ligand. The reported ligand expands the HaloTag toolbox by enabling in vivo labeling & providing capabilities for efficient affinity capture of protein variants from live cells rupress.org/jcb/article/...

Very happy to share this Perspective in @natrevimmunol.nature.com www.nature.com/articles/s41...

During obesity, the bone marrow is dysregulated to overproduce myeloid cells that drive systemic inflammation. Waterbury, Wang et al. @columbiacancer.bsky.social show that neutrophil migration is a key mediator of this process. rupress.org/jem/article/...

🔬 From JCB’s July issue (rupress.org/jcb/issue/22...): a PtK2 cell plated on a micropattern and injected with low doses of soluble actin (orange) and tubulin (blue) in order to generate speckles along the corresponding filaments. From @manuelthery.bsky.social group (rupress.org/jcb/article/...).

Congrats to Jing @jesszhiyuezhao.bsky.social‬ & @dnamystrom.bsky.social‬ on cool manuscript!!! Reveals how cell nucleus is a heterogeneous material, w chromatin organization controlling formation & phase behavior of nuclear condensates. #CellBiology #Chromatin #PhaseSeparation rdcu.be/evWxR

🧠🔬 A new microscope, ExA-SPIM, lets researchers zoom from molecules to whole brains, no slicing required. By combining tissue expansion with high-speed imaging, it captures stunning detail across entire mouse brains and even human samples. buff.ly/JlkKLcO

Antibody durability is influenced by interleukin-2 production by undifferentiated memory T helper cells and extensive B cell clonal expansion

SARCOMERES divide! Mechanism how muscles grow while contracting - daughter sarcomeres appear everywhere. Seen live, registered on our cover. Pioneered by @clementrodier.bsky.social @friedrich-group.bsky.social Ian Estabrook @ibdm.bsky.social $ by @hfspo.bsky.social www.science.org/doi/10.1126/...

Really excited to share this Perspective from John Danial covering how technology is moving the field of fluorescence microscopy toward structural biology. www.nature.com/articles/s41...

New: Pasolli, Meiring, Akhmanova et al. @utrechtuniversity.bsky.social describe tools to acutely relocate #vimentin intermediate filaments by inducibly coupling them to microtubule motors. rupress.org/jcb/article/... @joycemeiri.bsky.social @gijsjekoenderink.bsky.social #Technology #Cytoskeleton

this focused issue includes a great method by @alexandra-colin.bsky.social to make closed microwell in order to encapsulate your favorite reconstitution mix in compartments of controled size and shape. 🔴🟥🔻❤️

Our July issue is here! rupress.org/jcb/issue/22... The cover shows a confocal timelapse imaging of #Celegans one-cell embryo expressing fluorescently labeled plasma membrane, microtubules, chromosomes, and ZEN-4, a plus-end directed kinesin motor protein. By Adhikary et al. doi.org/10.1083/jcb....

How do cells link adhesion to protein synthesis? New findings suggest the protein p130Cas forms droplets that bud from focal adhesions and suppress translation, hinting at a novel mechanism for regulating cell state via phase separation. buff.ly/2I99ZTc

Cachexia is a devastating cancer comorbidity that impacts numerous cancer outcomes. @aneedasgupta.bsky.social, @jdoles.bsky.social et al. describe a novel metabolic pathway/target with clinical implications for the treatment of cachectic #PancreaticCancer patients. rupress.org/jem/article/...

Spindle morphology changes rapidly and dramatically at the meiosis to mitosis transition in early embryogenesis. Cantwell et al. @berkeleymcb.bsky.social uncover differential CK2 regulation of the RanGTP pathway of spindle assembly in meiosis & mitosis driving this change rupress.org/jcb/article/...