#OPENACCESS: Spatiotemporal dynamics of local anesthetic diffusion in nerve revealed by a 2D computational model. Vladimir Smrkolj, Jakob Kralj, Janez Mavri, and Nejc Umek.

#OPENACCESS: Annotating the x-ray diffraction pattern of vertebrate striated muscle. Natalia A. Koubassova, Debabrata Dutta, Weikang Ma, Andrey K. Tsaturyan, Thomas Irving, Raúl Padrón, and Roger Craig.

Cells rolling in microvessels = like shopping carts with varying wheel grip. Yonggang Li et al. (2025) link molecular bond dynamics to how fast cells “roll” in blood flow. Tiny stickiness = big traffic effects!

Special issue call for papers: Viral Biophysics.

The authors present initial experimental evidence detailing the biophysical mechanisms by which SARS-CoV-2 interferes with pulmonary surfactant function, highlighting the role of the S2 subunit of the viral spike protein in inducing this inhibition.

#OPENACCESS: Reactive oxygen species counteract zebrafish wound contraction and promote wound healing. Chang Ding, Linlin Li, Yueyang Wang, Hong-Anh A. Nguyen, Deva D. Chan, David M. Umulis, Adrian T. Buganza, and Qing Deng.

In the New and Notable "Milestone for the interpretation of muscle x-ray diffraction patterns," Anthony L. Hessel highlights the paper "Annotating the X-ray diffraction pattern of vertebrate striated muscle."

#OPENACCESS: Nayak et al. develop a computational whole-cell model revealing how actin-microtubule crosstalk drives cell polarity, shape, and migration behaviors.

#OPENACCESS: The study reveals key features of the different structural states of the 5 ‘and 3’ UTRs of the dengue virus during its replication, highlighting the adaptability of the UTR structures and contributing to the development of targeted drugs. buff.ly/dtWrfLJ

#OPENACCESS: MreB filaments in the elongasome modulate E. coli membrane curvature. Becca W.A. Baileeves, Anthony D.Q. Hoang, Timothy D.H. Bugg, and Phillip J. Stansfeld.

Special issue call for papers: Modeling Biology at Multiple Scales: From Macromolecules to Cells, Dedicated to Jie Liang.

In the New and Notable "A multimodal glimpse into the amyloid precursor protein intracellular domain ensembles," Veronica Isabel Dodero highlights the paper "Unraveling the conformational landscape of amyloid precursor protein intracellular domain."

Cells can switch vibes! Lin-Ru Qiao et al. (2025) show single cells shift from fluid-like to solid-like behavior—like going from jelly to jawbreaker depending on mechanical cues.

#OPENACCESS: Unraveling the conformational landscape of amyloid precursor protein intracellular domain. Nabanita Mandal and Marie Skepö.

#OPENACCESS: Calcium dynamics in small spaces: Lessons learned from modeling in dendritic spines. Kimberly J. McCabe, María Hernández Mesa, and Padmini Rangamani.

In the New and Notable "Single-cell memory matters in collective migration ," Ruihan Hou and Jinglei Hu highlight the paper "Time-dependent active force drives periodic reversal in collective cell migration."

#OPENACCESS: OsmoFold it, don’t guess it! New open-source tool predicts how osmolytes affect protein stability and folding.

Read about the cover art for the newly released issue of Biophysical Journal on the BPS Blog: www.biophysics.org/blog/flexibi...

Special issue call for papers: Martin Karplus: In Memoriam.

Special issue call for papers: Viral Biophysics.

#OPENACCESS: An application of segmented fluorescence correlation spectroscopy to investigate the dynamics of the PARP1 protein reveals its reduced mobility at DNA damage sites and unaltered PARP1 diffusion outside the DNA damage region. Link:

New study uses super-resolution imaging to study PIP2 clustering in mast cells, showing that antimicrobial CPC disrupts this organization and inhibits cell function at low (µM) doses.

#OPENACCESS: Think of FCS as filming a hummingbird’s wings—fast, tiny, and hard to see. Pandey et al. tested different super-resolution “slow-motion modes” to see which one turns that blur into crisp frame-by-frame motion. Their guide helps you pick the best mode to catch the flaps!

Special issue call for papers: Modeling Biology at Multiple Scales: From Macromolecules to Cells, Dedicated to Jie Liang.

#OPENACCESS: Assessing the role of all the relevant timescales that affect the observed photon emission, fluorescence lifetimes, rotational correlation times, and the chain dynamics in this integrated approach combining all-atom MD simulations with single-molecule FRET spectroscopy.

#OPENACCESS: Variational calculus approach to Zernike polynomials with application to FCS. Ivan Gligonov and Jörg Enderlein.

Special issue call for papers: Martin Karplus: In Memoriam.

#OPENACCESS: Imagine your microscope images are two overlapping constellations — one for protein A, one for protein Patra et al. use pair cross-correlation like a radar to measure how “in sync” the stars are across distances — it teases out true co-localization beyond just overlap!

Foust & Piston use multicolor fluorescence fluctuation spectroscopy to investigate GPCR–G protein interactions in live cells, finding no stable complexes. Read more to find how their study supports a collision-based model of signal transduction.

Read about the cover art for the newly released issue of Biophysical Journal on the BPS Blog: www.biophysics.org/blog/super-r...

#OPENACCESS: 3D localization of retrovirus assembly in the presence of structured background with deep learning. John Kohler, Kwang-Ho Hur, Elijah Wray, Jesse Donahue, Rayna Addabbo, Louis M. Mansky, and Joachim D. Mueller.

#OPENACCESS: Atomistic modeling of lysophospholipids from the Campylobacter jejuni lipidome. Astrid F. Brandner, Kahlan E. Newman, Jonathan W. Essex, and Syma Khalid.

Imagine chromatin as a tangled origami kite string in a breeze. Kadam et al. tracked how bits of that string jiggle over time and found that loop-extruding motors (like wind gusts) can dominate dynamics even when static folds look controlled by other interactions.

The study reveals that the N-terminal helix of Arf1, a GTPase, maintains the protein in the repressed state that is unique to Arf and Arf-like GTPases via overall stabilization of the native state, and no allosteric communication is involved with the switch region.

Special issue call for papers: Viral Biophysics.

#OPENACCESS: Bayesian analysis and efficient algorithms for single-molecule fluorescence data and step counting. Chiara Mattamira, Alyssa Ward, Sriram Tiruvadi Krishnan, Rajan Lamichhane, Francisco N. Barrera, and Ioannis Sgouralis.

Think of each embryo cell as a jelly-bean with a trampoline skin. Kotani et al. used AFM to press and release and map how “stiff” or “bouncy” each bean is during cleavage. They revealed a mechanical topography of the early embryo all in one map.

#OPENACCESS: Think the cell membrane flows like a fluid? It depends on scale! Sakuma et al. measure long-range viscosity in the early C. elegans embryo and find it is four orders of magnitude greater than short-range viscosity, likely due to the actin cytoskeleton.

An attempt to sample diverse conformations of the voltage-gated channel superfamily via AlphaFold2 predicts activated, deactivated, and intermediate states, and it also shows that the prediction of coupled conformational changes across functional domains is limited.

Special issue call for papers: Modeling Biology at Multiple Scales: From Macromolecules to Cells, Dedicated to Jie Liang.

In the New and Notable "Hunting for vesicles and chromosomes under time constraints," Maja Novak highlights the paper "Branching, crosslinking and decentralization of microtubules accelerates intracellular assembly."

Cytoskeletal contractions don’t just power movement. Sun et al. combine live-cell experiments and simulations to show that inhibiting cell-substrate adhesion spontaneously triggers diverse dynamic behaviours – from oscillations to protrusions to spreading.

#OPENACCESS: Advances in ligand-based surface engineering strategies for fine-tuning T cell mechanotransduction toward efficient immunotherapy. Ngoc Luu, Junru Liao, Yifei Fang, and Weiqiang Chen.

Read about the cover art for the newly released issue of Biophysical Journal on the BPS Blog: www.biophysics.org/blog/vortex-...

#OPENACCESS: Through MD simulations of β-endorphin31 and amyloid-β42 peptides, the authors demonstrate that maintaining a collapsed state is essential for aberrant oligomerization, underscoring the role of hydrophobic sequences in stabilizing aggregate structures.

#OPENACCESS: β-cell networks act like neighborhoods with quirky shops—each spot has its specialty, and together they form a modular city! Maja Duh et al. 2025 reveal how spatially bound heterogeneity organizes insulin-secreting communities.

Special issue call for papers: Martin Karplus: In Memoriam.

Combining structural analysis, docking, and atomistic simulations, this new study identifies a dynamic membrane-facing groove as a potential PIP2 binding site on TRPV4, disputing earlier claims of other proposed binding sites.

#OPENACCESS: Protein motion in a synapse is like cars in a roundabout—geometry dictates the traffic flow! Simon Dannenberg et al. 2025 show how shape sculpts protein mobility at these tiny junctions.

Special issue call for papers: Viral Biophysics.