"The result is a treadmill of constant activity with limited progress whereas core scholarly practices – such as reading, reflecting and engaging with others’ contributions – is de-prioritized." arxiv.org/pdf/2511.04820 Will the real scientists please stand up?

I'm pleased to announce 🍦 Icecream 🍨 v0.3! New features include: * Training on multiple tomograms (same training time, with linear increase in RAM) 🚀 * Logging and plotting of the loss function 📉 * The --scale option is now called --eq-weight for clarity 😉 We'd love to hear your feedback! 🙏🏽

Our new preprint is out 🥳🥳🥳 Henipaviruses, like Nipah and Hendra, package their genomes inside helical shells built by thousands of nucleoproteins. These nucleocapsids are essential to protect the viral RNA, but how do they ever let the polymerase in to read the sequence? 👇

Jeremy is an excellent scientist and leader. Highly recommended!

We are looking for a computational postdoc to work with us on new optimisation algorithms to make #RELION even better. Join our bubbly team at the @mrclmb.bsky.social in Cambridge, UK. 🤗 RTs appreciated. mrc.tal.net/vx/appcentre...

warwick.ac.uk/fac/cross_fa... I am advertising for a PhD position in my group. We'll be investigating how pathogenic RNA viruses replicate in the nucleus. The project will teach cryoEM/ET, virus culture, and in vitro assays. If you're interested reach out and I'd be happy to have a chat.

www.biorxiv.org/content/10.1... Really excited to have our work out in the world. CCHFV has a 450kDa L-Protein, about twice the size of L-Proteins from other segmented RNA viruses. We have used cryoEM and functional assays to begin to understand it this massive multifunctional protein works.

End-to-end protein design in the browser through evedesign. Generate and interactively explore designs in 2D/3D and export them as codon-optimized DNA. The underlying open source framework (released soon) is build to easily add new methods, more on that soon. 🌐 evedesign.bio

Neat!

Nice one Flo @schurlab.bsky.social !

Our aim was to create a kit to produce in-house 1.2 holey carbon cryoEM grids, in three simple steps, at less than half of the prize, and without due times. I think we are there. Academic teams that want to know more, receive training or order a kit, please contact [email protected].

Confidence-guided cryo-EM map optimisation with LocScale-2.0 www.biorxiv.org/content/10.1101/2025.09.11.674726v1 #cryoem

We finally wrote up the paper for LocScale-2.0. edu.nl/pvhxv Please try it out. LocScale is evolving & we are actively building in new features and improvements. Your feedback will help us shape the next steps. Code: cryotud.github.io/locscale/ Work by @alok-bharadwaj.bsky.social and Reinier.

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

Ben Vollmer and Kay Grunewald & team @cssbhamburg.bsky.social and the beautiful structure along important insights for herpesvirus entry and membrane fusion.

Researchers from CSSB & @mpi-nat.bsky.social have generated a highly potent nanobody that neutralizes a protein essential for the herpes virus infection. Published in @nature.com, the findings promise new therapies to treat & prevent infections @leibnizliv.bsky.social @uni-hamburg.de t1p.de/cxt7a

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators. www.nature.com/articles/s41...

One week left to apply for a fully funded PhD position in my group! Please repost. jobrxiv.org/job/max-plan...

Very pleased to have our new work out where we perform a structure/function study on the bornavirus polymerase complex with fantastic coauthors. Have a read www.nature.com/articles/s41... @warwicklifesci.bsky.social @loiccarrique.bsky.social @franziskaguenl.bsky.social

New Title Alert: Tomcat- a web-based tool designed for cataloging and managing tomography data providing an easy way to view, annotate, and organize tomograms. Learn more here: buff.ly/75XbvPv #SBGrid #SBGridSoftware #StructuralBiology

Twist and Scout: Analysis and Curation of Particles in Cryo-Electron Tomography Using TANGO www.biorxiv.org/content/10.1101/2025.07.17.665296v1 #cryoem

Latest preprint from the lab, many years in the making! By combining #cryoEM with #AlphaFold3 modelling, we propose that norovirus NS3 forms a transmembrane RNA translocase. This could have big implications for our understanding of viral replication & assembly (🧵) www.biorxiv.org/content/10.1...

Proud to share our latest paper. doi.org/10.1016/j.cr... Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue. Structural biology in tissue is well and truly here! @rosfrankinst.bsky.social

Chromatic aberration (Cc) corrected cryo-EM: The structure of pseudorabies virus (PRV) using both zero-loss and energy loss electrons pubmed.ncbi.nlm.nih.gov/40499325/ #cryoem

🎀✨Recording now available ✨🎀 youtu.be/jtDunWK8g1o?...

We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social! www.biorxiv.org/content/10.1... #Chromatin #Asgard #Archaea #cryoEM

If u ever had to depict proteins as schematics in papers, u know the dilemma: how to create a pretty “blob”? If structure ends up big & central, u’ll render it with PyMol et al, but for a truly schematic depiction u want a biorender-style icon, but for YOUR protein. Think I’ve finally found a way.

In the Bay Area? Love computational cryo-EM/ET? Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community! Details and registration at frostbyte-cryoem.github.io Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!

⚠️ Discovery of a new mode of viral transmission: Deltaviruses spread through a viral Trojan Horse ! These viral satellites that do not encode their own glycoproteins, are in fact physically encapsulated within their helper virus particles! www.biorxiv.org/content/10.1... A thread 1/14

1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.

Congrats @doryenbubeck.bsky.social !

Great stuff @tomo.science ! Quite inspiring 🥰

TomoCPT is a transformer-based solution that reformulates particle detection in cryo-ET as a centroid-prediction task using Gaussian labels #CryoET #ParticlePicking #SubTomogramAveraging doi.org/10.1107/S205...

ProPicker: Promptable Segmentation for Particle Picking in CryogenicElectron Tomography [new] Cryo-ET: 3D seg. picks particles via prompts.

Injecting arbitrary experimental data into AlphaFold has been a missing feature. With high-throughput crystallography yielding 1000s of structures/year (@covid_moonshot) & cryoET making 😍 but low-res in situ models, it's also become a necessary one for scaling up structural biology. Hence ROCKET👇!

I don't want to brag (mostly because I had nothing to do with it), but that's a really cool #npjViruses Review with everything you wanted to know about #giantviruses: www.nature.com/articles/s44... #virus

I’ll have more to say about this later, but please have a look, and I hope the #TeamTomo community finds good uses for these 1829 Chlamy tomograms and annotations 🧪🧶🧬🌾🔬

Check out the magnificent #baculovirus structure! The unique virion architecture and structural atlas of hallmark proteins place baculo-like viruses into a separate new realm. Many insights into baculo biology and evolution. Collab with the group of Fasséli Coulibaly. www.science.org/doi/10.1126/...

#TeamTomo #SciArt 🧪

1/ A new series of #CryoSPARC tutorial videos from this year’s S2C2 #cryoEM image processing workshop are now online! These videos will be interesting to all users and especially those newer to #cryoEM. www.youtube.com/playlist?lis...

Excited to share our work describing a series of structures that allow us to propose a new model for Hantavirus genome replication by the viral L-protein. Another great experience with @loiccarrique.bsky.social @ben-nilsson-payant.bsky.social @efodor.bsky.social Jon Grimes. doi.org/10.1371/jour...

Gotta love finding soccer balls in tomograms ⚽ 😂

I am about to be recruiting two post-docs to work on cryoEM and cryoET of amyloid aggregates... Anyone interested please drop me a line while the wheels of our HR department turn.............

Here is a starter pack for CryoEM/ET enthousiasts To be completed go.bsky.app/JmQksC

Starter packs are great for a maximally twitter-like experience, but I've been really into feeds, which let me sort by content rather than by specific people. Here's one for #teamtomo bsky.app/profile/did:...

My half-hearted attempt to start a structural virology starter pack. Could be useful for conference organisation in the future! 😀 go.bsky.app/Qxv95BL Comment below if you'd like me to add you, or anyone you recommend! #virology #cryoEM

STOPGAP, an open-source package for subtomogram averaging, is presented together with detailed descriptions of the image-processing algorithms that it uses #STOPGAP #CryoET #OpenSource t.co/jXgHHu6GMD

From @efodor.bsky.social www.nature.com/articles/s41... Beautiful structures explaining the story behind some mutations which are relevant at the moment for cow flu. Also, nice to see that subtle differences between the FluC and FluA structures determine which mutations make a difference.

Our latest preprint describes a set of mutations that can be used on (in principle) any Fab to make it much more rigid. Postdoc Jennifer Kung's results demonstrate that such #rigidFabs can enable #cryoEM of small (~25kD) targets at good spatial resolutions (~2.7 Å).

Disulfide constrained Fabs overcome target size limitation for high-resolution single-particle cryo-EM https://www.biorxiv.org/content/10.1101/2024.05.10.593593v1