🌱 Using ‘compelling’ methods, including #CryoET, researchers mapped spinach thylakoid membranes at single-molecule precision, revealing how photosynthetic complexes are organised and settling long-standing debates on chloroplast architecture. buff.ly/j3TSIkn

A molecular-resolution look into the near-native architecture of the spinach chloroplast🌱. This one was a long time in the oven, but we're happy to finally share our "version of record". What long-standing debates did we settle? Check back for a short thread🧵 on Monday. #TeamTomo #PlantScience 🧪🧶🧬🔬🌾

Mycobacterium tuberculosis forms nitrate-induced intracytoplasmic membranes (MIMs) that rewire metabolism & dampen inflammation during infection, uncovering a new layer of bacterial adaptation! ✍️ @camimille.bsky.social @enningalab.bsky.social @pasteur.fr 📖 shorturl.at/9kbDM

After years of hard work and months of growing MTB @camimille.bsky.social submitted her story ! Check this out if you wanna discover new intrabacterial structures and their function ! 🦠🔬✨

How complex functions, with important physiological and evolutionary impacts get repeatedly and efficiently transferred across genomes? That’s what we explored using one of the fastest-evolving loci in Bacteria: the capsule locus. The paper: www.biorxiv.org/content/10.1... Thread👇

Delighted to share our recently published work! Ever wondered how Klebsiella (and others) deals with capsule production’s costs ? The paper: journals.asm.org/doi/10.1128/... Thread👇

🦠 Mycobacteria aren't just tough, they're interior designers! 🦠 Check out our latest #preprint 🚨, led by our PhD student @camimille.bsky.social , where we show that #Mycobacterium tuberculosis builds secret cytoplasmic membranes to adapt its metabolism and sneak past immunity 🤫 Thread coming soon!

New preprint from the lab! Great work led by @camimille.bsky.social #mycobacterium #TB #cryoET

Happy to share that our story on the bacterial archaellum was published today in @natmicrobiol.nature.com www.nature.com/articles/s41... Congrats to the authors: @sshamphavi.bsky.social @loumollat.bsky.social @mariejoest.bsky.social Najwa Taib and @sgribaldo.bsky.social

Story on our Asgard microtubule paper by Nikki Greenwood: www.quantamagazine.org/tiny-tubes-r... Thanks to all interviewees🎤

Peptidoglycan turnover promotes active transport of protein through the bacterial cell wall https://www.biorxiv.org/content/10.1101/2025.09.12.675941v1

New paper out, with a contribution from Dom Gurvic and I; led by Andy Lovering and @lalouxlab.bsky.social: www.nature.com/articles/s41...

Genetically encoded FerriTag as a specific label for cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40925364/ #cryoem

Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here: meetings.embo.org/event/26-in-...

Check out 🧐 our new preprint 📜: www.biorxiv.org/content/10.1... Congrats🎉 to @vasilgaisin.bsky.social and Corina Hadjicharalambous, who studied contractile injection systems directly in hot spring♨️ bacterial mats🦠 #microsky #teamtomo #Chloroflexota

Tn7 family bacterial transposons known for tightly controlled target site selection are distributed across Asgard and archaeal groups https://www.biorxiv.org/content/10.1101/2025.07.27.667033v1

Ready to lead pioneering research that bridges systems-level investigations of biological systems to molecular mechanism? The EMBL Molecular Systems Biology Unit in Heidelberg is hiring a Group Leader!

🚨 Only 6 days left to apply! Cryo-Electronic Microscopy – Pasteur Course Master cryo-EM fundamentals through a fully hands-on, 2-week training at the Institut Pasteur. 📅 September 29 – October 10, 2025 🗣️ Course in English ⌛ Application deadline: July 31, 2025 👉 bit.ly/4o1KD9H @pasteur.fr

my little zebra friends

Excited and honored to be joining the EMBO Member community 🥳 Congratulations also to all the other newly elected members! -Martin

Diving “Google earth mode 🌍” in a Shigella infected cell 🔬🔎 Together with @m-albert.bsky.social

Cool result: Transposon insertion sequencing reveals novel hypermutator genes in Acinetobacter baumannii "Exposure of a transposon insertion mutant library to extended weak antibiotic selection ... revealed genes that indirectly increased fitness due to elevated general mutation rates"

Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...

🚨 New preprint! Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM www.biorxiv.org/content/10.1...

After 5 amazing years @pilhoferlab.bsky.social I'm starting my lab @embl.org Grenoble this November embl.org/wollweber We'll image Asgard archaea and many other strange microbes ( #archaeasky, #protistsonsky..) to understand eukaryogenesis First job ad: #teamtomo scientist!🔬❄️ tinyurl.com/2rdu2ze6

Super cool 🧬

New EMBO J 𝘔𝘺𝘤𝘰𝘣𝘢𝘤𝘵𝘦𝘳𝘪𝘶𝘮 𝘵𝘶𝘣𝘦𝘳𝘤𝘶𝘭𝘰𝘴𝘪𝘴 uses “on-off switch” to pause or restart its growth DarT tags DNA to halt replication, DarG removes the tag to resume activity Stop-start mechanism helps TB evade antibiotics and immune clearance, establish chronic infection www.embopress.org/doi/full/10....

Tuning the interaction of a ParA-type ATPase with its partner separates bacterial organelle positioning from partitioning https://www.biorxiv.org/content/10.1101/2025.05.22.655647v1

Intralysosomal pathogens differentially influence the proteolytic potential of their niche https://www.biorxiv.org/content/10.1101/2025.05.21.655381v1

A Conserved Mycobacterial Nucleomodulin Hijacks the Host COMPASS Complex to Reprogram Pro-Inflammatory Transcription and Promote Intracellular Survival https://www.biorxiv.org/content/10.1101/2025.05.21.655295v1

New pre-print in the lab! Beautiful work by Francisco Garcia-Rodriguez in collab with @kamovalenz.bsky.social and @joaquinbernal.bsky.social : when macrophages are provided with specific nutrients, Salmonella can bypass its requirement of the T3SS to replicate, a process that occurs within the SCV

Bacteria have a simple yet potent mechanism that controls their sensitivity to environmental stimuli. A new study by researchers led by @erikvannimwegen.bsky.social @biozentrum.unibas.ch shows: the slower cells grow, the more sensitively they respond to their environment.

Our latest paper is out! Congratulations to @leaswistak.bsky.social et al for this amazing #cryoET work that elucidates the initial stages of #T3SS dependent vacuolar damage during #Shigella infection. We will do a deep-dive later! journals.plos.org/plosbiology/...

How do intracellular #bacteria breach vacuoles to enter the host cytosol? @leaswistak.bsky.social @matthijnvos.bsky.social @enningalab.bsky.social &co show that #Shigella uses its T3SS system to damage endomembranes via mechanoporation to initiate cytosolic access @plosbiology.org 🧪 plos.io/4jXrr9Z

An atlas of metabolites driving chemotaxis in prokaryotes This database compiles the effects (attractant, repellent or neutral) of 926 compounds and the chemotactic behaviour of 394 strains #ChemicalBiology #Microbiology

You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software! Hope it will be useful !

Join the Blokesch lab (@EPFL) as a postdoc in Molecular Microbiology 🤩 Study Vibrio cholerae defense systems (phage/plasmid) in a dynamic, collaborative environment in Lausanne close to Lake Geneva in 🇨🇭! Please visit my lab's webpage for details about the position and how to formally apply.

🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics. 🔗 Preprint: doi.org/10.1101/2024... 🔗 Code: github.com/CellArchLab/... 🧵(1/6) #TeamTomo

A highly stable monomeric red fluorescent protein for advanced microscopy www.nature.com/articles/s41...

Hurray, it is finally out! Meet bacteriophage Bxb1 - the subject of my first full-phage cryo-EM study. My structures are beautifully complemented by the Park Lab’s cryo-ET analysis, shedding light on mycobacteriophage structural changes during infection. www.sciencedirect.com/science/arti...

New postdoc position in our lab (2 y+): evolutionary genomics of integrons and MGEs with focus on vibrio-phage interactions. Great environment @pasteur.fr for science, career building. Super collaborators @celineloot.bsky.social @amazeld.bsky.social @fredoleroux.bsky.social 3 weeks to apply!

The #GRCMyco2025 group photo is now in everyone's mygrc folder! It was VERY sunny....

Join our favorite microbiology meeting! Fantastic talks, poster sessions, new technologies, and all the hot areas of microbiology in a great environment - experts in cell & systems biology, microbiomes, environment & evolution, pathogenesis, phage & antibiotics DON’T MISS abstract deadline, April 1

ICYMI: New online! Ecology, global diversity and evolutionary mechanisms in the Mycobacterium tuberculosis complex

Please RT. Post-doc opportunity alert! 💥 Come join our team (thelowlab.org) at Imperial, London, working on bacterial secretion systems. The position is funded by the Wellcome Trust. For more details and to apply please see www.imperial.ac.uk/jobs/search-...

ahhhh amazing

Now online! Microtubules in Asgard archaea

Asgard archaea have actin - but what about microtubules? Where do they come from? 🧐 Our new paper www.cell.com/cell/fulltex... by @xujwet.bsky.social & @florianwollweber.bsky.social, in collaboration with the Schleper & Wieczorek labs, describes tiny Asgard microtubules! #TeamTomo #ArchaeaSky 1/6

Clostridioides difficile vs. Oxidative Stress again! Take a look at how this strict anaerobe faces ROS through its arsenal of detoxification enzymes. A key adaptation for its survival! 🔬✨ doi.org/10.1128/mbio...

The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @science.org 🎉 You can find the full story here www.science.org/doi/10.1126/... @verenaresch.bsky.social did a wonderful job on the cover and animation, she really brought it to life 👩‍🎨