We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇

We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better. Preprint: www.biorxiv.org/content/10.6... Code: github.com/warpem/miss-... 🧶 Thread:

Across scales from cells🦠 to atoms⚛️ – We reveal how anaerobic #bacteria break down very stable aromatic compounds found e.g. in oil spills 🛢️ #Bioremediation #TeamTomo 🧪 🧶🧬 🔬 Awesome collab with Lena, Matthias, @schullerjm.bsky.social @rnfr2d2.bsky.social @tomaspascoa.bsky.social @tamb-o.bsky.social

🧬 EMBO Workshop – The complexity of mycobacterial infections: from research to real-world impact 📅 14–18 Sept 2026, Paris Tuberculosis & non-tuberculous mycobacteria at the heart of discussions: evolution, host–pathogen interactions, drug resistance. 🔗 meetings.embo.org/event/26-myc...

Let's get ready for a full month of training in image analysis in @pasteur.fr , this summer! Are you interested in learning how to do bioimage analysis? For light microscopy? for cryo-EM? And for cellular cryo-EM? Stay tuned, Pasteur offers access to THREE courses, this June. 👇🎓

I'm pleased to announce 🍦 Icecream 🍨 v0.3! New features include: * Training on multiple tomograms (same training time, with linear increase in RAM) 🚀 * Logging and plotting of the loss function 📉 * The --scale option is now called --eq-weight for clarity 😉 We'd love to hear your feedback! 🙏🏽

Glushkova, Böhm, & Beck @maxplanck.de develop a publicly available computational method to measure the thickness of biological membranes in cryo-electron tomograms. Analysis of algae & human cells reveals systematic membrane thickness variations within & across organelles rupress.org/jcb/article/...

We’re excited to announce the incredible speaker line-up for the Prokaryotic Cell Biology Conference! Join the leading researchers as they explore the latest advances in Nanoimaging, Cell Growth and Cellular Structures. Join our speakers & register today! ➡️https://bit.ly/47rtkbw #ProkaryoticCellBio

🧐Checkout our preprint revealing the stepwise firing💥 mechanism of a Contractile Injection System @xujwet.bsky.social&@chipericson.bsky.social trapped the complex by structure-guided engineering🧪 in multiple intermediate states and imaged them by multimodal #cryoEM❄️🔬 www.biorxiv.org/content/10.1...

I'm recruiting a postdoc to figure out how aerobic methanotrophic bacteria oxidise methane in anoxic freshwater sediment environments: international.au.dk/about/profil... Come to Aarhus University in Denmark and work on this 2-year project funded by @villumfonden.bsky.social!

✨Excited that the main project of my PhD is now available as a pre-print on #bioRxiv Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo 🔗 www.biorxiv.org/content/10.1...

Check out our new preprint on an integrated pipeline combining in situ #cryo-ET with MALDI #MSImaging for single-cell identification and classification from previously analysed EM-grids. Link: www.biorxiv.org/content/10.1...

Latest from ours: www.cell.com/cell-reports... This is two stories in one: a case study/cautionary tale on developing genetic tools in new organisms, and the first hint at a gene regulatory network for choanoflagellate multicellular development (which turn out to involve a Hippo/YAP/ECM loop!) A 🧵

Cardiolipin dynamics promote membrane remodeling by mitochondrial OPA1 pubmed.ncbi.nlm.nih.gov/41027961/ #cryoem

EXCITING NEWS!! We're searching for a new professor colleague at the Biozentrum🇨🇭. Ideal candidates are exploring novel biological questions with structure, biophysics, or high-resolution imaging. 🧪 🧶🧬 🔬 The call closes Nov 3. Please get in touch if you would like more information. Come join us! 👩‍🔬🤓

We will have soon an Open position for a PhD thesis position to work on bacterial secretion systems ! Stay tuned !!

🚨New paper out! #MicroSky Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival. We expanded its genetic toolbox: 🧬promoters to fine-tune expression 🧬IPTG-inducible system 🧬CRISPRi for rapid knockdown bit.ly/46GUn2c 1/4

Cyril Anjou, 2e candidat au prix de thèse nous a présenté ses travaux sur les rôles physiologiques des systèmes thioredoxine de Clostridioides difficile, impliqués dans les étapes du cycle de vie de la bactérie, ainsi que sur l’intérêt thérapeutique potentiel de la modulation de leur expression.🦠

Excited to share Wendy Le Mouëllic’s PhD work, now published in @pnas.org! It reveals that M. tuberculosis depends on inorganic sulfate import to survive inside host cells—fueling essential processes such as redox balance and stress resistance. Huge congrats to Wendy & colleagues! shorturl.at/WbFQC

Time for some insights into our latest #preprint Using cryo–electron tomography, we peeked inside different #Mtuberculosis strains, and a couple of other mycobacteria.🧐 Surprise: some species, especially the host-adapted ones, build intracytoplasmic membranes (we call them MIMs)! 🦠

New PrePrint from the Vecchiarelli and Mosalaganti (@shyamalm.bsky.social) labs! 🧵 Collaboration with the Giessen and Pi labs Congratulations to co-first authors Y Hoang (@hoanghamyy.bsky.social) and Pankaj Jadhav! www.biorxiv.org/content/10.1...

🌱 Using ‘compelling’ methods, including #CryoET, researchers mapped spinach thylakoid membranes at single-molecule precision, revealing how photosynthetic complexes are organised and settling long-standing debates on chloroplast architecture. buff.ly/j3TSIkn

A molecular-resolution look into the near-native architecture of the spinach chloroplast🌱. This one was a long time in the oven, but we're happy to finally share our "version of record". What long-standing debates did we settle? Check back for a short thread🧵 on Monday. #TeamTomo #PlantScience 🧪🧶🧬🔬🌾

Mycobacterium tuberculosis forms nitrate-induced intracytoplasmic membranes (MIMs) that rewire metabolism & dampen inflammation during infection, uncovering a new layer of bacterial adaptation! ✍️ @camimille.bsky.social @enningalab.bsky.social @pasteur.fr 📖 shorturl.at/9kbDM

After years of hard work and months of growing MTB @camimille.bsky.social submitted her story ! Check this out if you wanna discover new intrabacterial structures and their function ! 🦠🔬✨

How complex functions, with important physiological and evolutionary impacts get repeatedly and efficiently transferred across genomes? That’s what we explored using one of the fastest-evolving loci in Bacteria: the capsule locus. The paper: www.biorxiv.org/content/10.1... Thread👇

Delighted to share our recently published work! Ever wondered how Klebsiella (and others) deals with capsule production’s costs ? The paper: journals.asm.org/doi/10.1128/... Thread👇

🦠 Mycobacteria aren't just tough, they're interior designers! 🦠 Check out our latest #preprint 🚨, led by our PhD student @camimille.bsky.social , where we show that #Mycobacterium tuberculosis builds secret cytoplasmic membranes to adapt its metabolism and sneak past immunity 🤫 Thread coming soon!

New preprint from the lab! Great work led by @camimille.bsky.social #mycobacterium #TB #cryoET

Happy to share that our story on the bacterial archaellum was published today in @natmicrobiol.nature.com www.nature.com/articles/s41... Congrats to the authors: @sshamphavi.bsky.social @loumollat.bsky.social @mariejoest.bsky.social Najwa Taib and @sgribaldo.bsky.social

Story on our Asgard microtubule paper by Nikki Greenwood: www.quantamagazine.org/tiny-tubes-r... Thanks to all interviewees🎤

Peptidoglycan turnover promotes active transport of protein through the bacterial cell wall https://www.biorxiv.org/content/10.1101/2025.09.12.675941v1

New paper out, with a contribution from Dom Gurvic and I; led by Andy Lovering and @lalouxlab.bsky.social: www.nature.com/articles/s41...

Genetically encoded FerriTag as a specific label for cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40925364/ #cryoem

Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here: meetings.embo.org/event/26-in-...

Check out 🧐 our new preprint 📜: www.biorxiv.org/content/10.1... Congrats🎉 to @vasilgaisin.bsky.social and Corina Hadjicharalambous, who studied contractile injection systems directly in hot spring♨️ bacterial mats🦠 #microsky #teamtomo #Chloroflexota

Tn7 family bacterial transposons known for tightly controlled target site selection are distributed across Asgard and archaeal groups https://www.biorxiv.org/content/10.1101/2025.07.27.667033v1

Ready to lead pioneering research that bridges systems-level investigations of biological systems to molecular mechanism? The EMBL Molecular Systems Biology Unit in Heidelberg is hiring a Group Leader!

🚨 Only 6 days left to apply! Cryo-Electronic Microscopy – Pasteur Course Master cryo-EM fundamentals through a fully hands-on, 2-week training at the Institut Pasteur. 📅 September 29 – October 10, 2025 🗣️ Course in English ⌛ Application deadline: July 31, 2025 👉 bit.ly/4o1KD9H @pasteur.fr

my little zebra friends

Excited and honored to be joining the EMBO Member community 🥳 Congratulations also to all the other newly elected members! -Martin

Diving “Google earth mode 🌍” in a Shigella infected cell 🔬🔎 Together with @m-albert.bsky.social

Cool result: Transposon insertion sequencing reveals novel hypermutator genes in Acinetobacter baumannii "Exposure of a transposon insertion mutant library to extended weak antibiotic selection ... revealed genes that indirectly increased fitness due to elevated general mutation rates"

Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...

🚨 New preprint! Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM www.biorxiv.org/content/10.1...

After 5 amazing years @pilhoferlab.bsky.social I'm starting my lab @embl.org Grenoble this November embl.org/wollweber We'll image Asgard archaea and many other strange microbes ( #archaeasky, #protistsonsky..) to understand eukaryogenesis First job ad: #teamtomo scientist!🔬❄️ tinyurl.com/2rdu2ze6

Super cool 🧬

New EMBO J 𝘔𝘺𝘤𝘰𝘣𝘢𝘤𝘵𝘦𝘳𝘪𝘶𝘮 𝘵𝘶𝘣𝘦𝘳𝘤𝘶𝘭𝘰𝘴𝘪𝘴 uses “on-off switch” to pause or restart its growth DarT tags DNA to halt replication, DarG removes the tag to resume activity Stop-start mechanism helps TB evade antibiotics and immune clearance, establish chronic infection www.embopress.org/doi/full/10....

Tuning the interaction of a ParA-type ATPase with its partner separates bacterial organelle positioning from partitioning https://www.biorxiv.org/content/10.1101/2025.05.22.655647v1

Intralysosomal pathogens differentially influence the proteolytic potential of their niche https://www.biorxiv.org/content/10.1101/2025.05.21.655381v1