I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪

A molecular-resolution look into the near-native architecture of the spinach chloroplast🌱. This one was a long time in the oven, but we're happy to finally share our "version of record". What long-standing debates did we settle? Check back for a short thread🧵 on Monday. #TeamTomo #PlantScience 🧪🧶🧬🔬🌾

1/14 Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵 Free article: go.nature.com/4oRUC1q

In our new article, we show how P. aeruginosa, a major cause of chronic respiratory infections in #cysticfibrosis (CF), uses the Type VI Secretion System #T6SS and specific #toxins to eliminate competing bacteria www.cell.com/cell-reports... #cryoEM @dshatskiy.bsky.social @jakecolautti.bsky.social

Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social www.biorxiv.org/cgi/content/... @mrclmb.bsky.social @wellcometrust.bsky.social

Postdoc program at the MDC in Berlin, @mdc-berlin.bsky.social : take a look 👇👇 For this call, collaborative projects between two groups will be funded. Take a look at the call and the groups and drop me an email if interested. Share with colleagues too! www.mdc-berlin.de/postdocs#t-g...

Director at Max Planck - a unique position! The Open Call for Expressions of Interest in Max Planck Directorships is open now and can be submitted by the 31st of October 2025. ➡️ mpg.de/directors - Please share the Open Call among potential candidates.

New preprint: In situ Architecture of #Plasmodesmata. Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores. 🔗 doi.org/10.1101/2025... 🧪🧵1/n #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM

And here it is now in print! www.cell.com/cell-reports... With a front cover splash 😍

Our entrance into protein design! Inverse folding steered by external sources of information, and for multiple conformations. By the amazing @kaiyi94.bsky.social and @kjamali.bsky.social! Download our code and try it yourself. 🥳

This is cool. CryoEM showing COPI coat containing GOLPH3, together with functional data suggests the mechanism for cargo retention/transport. The mechanistic basis of cargo selection during Golgi maturation Taylor, Zubkov, Ciazynska et al. doi.org/10.1101/2025...

Structure of the virulence-associated Neisseria meningitidis filamentous bacteriophage MDAphi Out in @pnas.org Congrats @janboehning.bsky.social @abultarafder.bsky.social Miles Graham @mathieucoureuil.bsky.social @mrclmb.bsky.social @hopitalnecker.bsky.social www.pnas.org/doi/10.1073/...

Applications close this Friday, 20th June.

Congratulations! The #ShawPrize in Life Science & Medicine 2025 is awarded to Wolfgang Baumeister for his pioneering development & use of cryo-ET. ❕https://www.shawprize.org/laureates/2025-life-science-medicine/ #CryoEM #cryoET #Award

We are happy to release LocScale2.0: a tool for context-aware, confidence-weighted cryoEM map optimisation. Taking two half maps as input, LcoScale-2.0 produces feature-enhanced maps along with a robust confidence score that guides objective map interpretation. cryotud.github.io/locscale/

Super happy to announce that together with @stefvass.bsky.social , we will have the honor to join 1st authors Florian Wernert and @moparthisk.bsky.social to present our recent work (www.science.org/doi/10.1126/...) to the French Academy of Sciences @academiesciences.bsky.social!

Congratulations to LMB Group Leaders Leo James, Greg Jefferis & Marta Zlatic who have been elected Fellows of the @royalsociety.org! Congratulations also to the #LMBAlumni joining them: John Briggs, Graham Hatfull & Baljit Khakh. Read more: www2.mrc-lmb.cam.ac.uk/leo-james-gr... #LMBNews

John Briggs has been elected to the Fellowship of the Royal Society, the UK’s national academy of sciences. Congratulations to him and the other outstanding researchers from across the world! ❕https://royalsociety.org/news/2025/05/new-fellows/ @briggsgroup.bsky.social ‪@royalsociety.org

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics. 🔗 Preprint: doi.org/10.1101/2024... 🔗 Code: github.com/CellArchLab/... 🧵(1/6) #TeamTomo

New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇

www.biorxiv.org/content/10.1... NEW PREPRINT ALERT! Thanks to all involved!

I am thrilled to announce my and @giuliazanetti.bsky.social's latest review "Mechanisms of COPII coat assembly and cargo recognition in the secretory pathway" in @natrevmcb.bsky.social with @springernature.com. Check it out here! rdcu.be/eeY1J #COPII #CryoEM #TeamTomo #MembraneTrafficking #ERES

Super excited to share our latest work on deciphering the #Ubiquitin Code “𝗨𝗯𝗶𝗥𝗘𝗔𝗗 𝗱𝗲𝗰𝗶𝗽𝗵𝗲𝗿𝘀 𝗽𝗿𝗼𝘁𝗲𝗮𝘀𝗼𝗺𝗮𝗹 𝗱𝗲𝗴𝗿𝗮𝗱𝗮𝘁𝗶𝗼𝗻 𝗰𝗼𝗱𝗲 𝗼𝗳 𝗵𝗼𝗺𝗼𝘁𝘆𝗽𝗶𝗰 𝗮𝗻𝗱 𝗯𝗿𝗮𝗻𝗰𝗵𝗲𝗱 𝗞48 𝗮𝗻𝗱 𝗞63 𝘂𝗯𝗶𝗾𝘂𝗶𝘁𝗶𝗻 𝗰𝗵𝗮𝗶𝗻𝘀” @cp-molcell.bsky.social 1/8 www.cell.com/molecular-ce...

We're on the cover of @science.org this week. Awesome work by @florentwaltz.bsky.social. #CryoET is reaching crazy resolutions inside cells, and this is just the beginning. Fantastic interpretation of the proton motive force by @verenaresch.bsky.social. Always a pleasure making #SciArt with you!🧪🧶🧬🌾

Wowww! Congrats Florent and coauthors!

Our methods paper made it to bioRxiv: doi.org/10.1101/2025... Full #cryoET workflows for #plant tissues. Plus some general tricks and tweaks on serial Lift-Out and cryo-FLM. 🧪🧵1/5 #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM #CLEM

I am very happy to announce that we solved the in-virus #cryoEM structure of the #HIV matrix protein. The structure helped us to resolve a long mystery in HIV biology. A🧵1/5 Read more in Nature: www.nature.com/articles/s41... 🧪

Mature #HIV-1 contains >2000 copies of “spacer peptide 2”, but we didn’t know why. We now see that SP2 binds the matrix protein and triggers the change into its mature arrangement. Summary video from @margotriggi.bsky.social

To all #TeamTomo #CryoET and #Chlamydomonas aficionados: we have updated EMPIAR-11830 with a bug fix concerning some cryo-CARE denoised tomos as well as additional files and metadata! 🎉👩🏽‍💻 www.ebi.ac.uk/empiar/EMPIA... A little thread about what's new... 🧵 1/n

The @briggsgroup.bsky.social alongside teams from @uniheidelberg.bsky.social and @yalemicropath.bsky.social uncover elusive role of #HIV-1 component SP2. Detailed 3D views of viruses by #cryoEM show how SP2 helps the virus matrix to mature. ❕Read the paper @nature.com www.nature.com/articles/s41...

Scaling data analyses in cellular cryoET using comprehensive segmentation [new] Comprehensive segmentation of cryoET data enables ontology-based voxel ID, used for context-aware particle picking and faster template matching.

I am happy to be a part of this effort and looking forward to the discoveries this dataset will enable! If you work with this dataset in the future, please add any annotations to the GitHub repository github.com/Chromatin-St... Thanks @paloha.bsky.social for spearheading the efforts with this!

Finally out! Working with this team was a fantastic learning experience. Also I was excited to determine the in situ clathrin structure for this project! That’s my first EM-structure upload to EMDB. Can I tell my mom I am a structural biologist now? 😁 #cryoET

Latest from Baumeister & @lottesogaard.bsky.social labs: The PomXYZ complex and its role in bacterial cell division. www.pnas.org/doi/10.1073/... #teamtomo #cryoET #cryoEM

I’ll have more to say about this later, but please have a look, and I hope the #TeamTomo community finds good uses for those 1829 Chlamy tomograms 🧪🧶🧬🌾🔬

More than 1'800 tomograms available for the community. Towards visual proteomics using our favorite organism Chlamy! Check out the preprint: www.biorxiv.org/content/10.1... Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com #TeamTomo

Our new preprint from Briggs lab is out! We solved the in-virus #cryoEM structure of the HIV matrix protein by SPA. The structure led us to a discovery resolving a long mystery in HIV biology – a peptide SP2 triggers the maturation of the HIV matrix domain. A 🧵 1/x Paper: tinyurl.com/c7szhtfa

Here is a starter pack for CryoEM/ET enthousiasts To be completed go.bsky.app/JmQksC