In our new article, we show how P. aeruginosa, a major cause of chronic respiratory infections in #cysticfibrosis (CF), uses the Type VI Secretion System #T6SS and specific #toxins to eliminate competing bacteria www.cell.com/cell-reports... #cryoEM @dshatskiy.bsky.social @jakecolautti.bsky.social

Thrilled to be one of 12 awardees of the prestigious #ERCStG from the LS1 panel this year! Over the next five years, we’ll study how intracellular bacterial pathogens #Listeria and #Shigella manipulate #actin to spread infection, with the aim of finding ways to stop them. Exciting times ahead!

🚨 preprint 2️⃣ this month: our (purely experimental🧪) venture into #ChemBio We prouldy present: ADD-tagging of proteins (or "ADDing") —a super convenient enzymatic technique to install click chemistry handles on proteins. Led by superstar @wahyuwidodo.bsky.social www.biorxiv.org/content/10.1... A 🧵👇🏽

If u ever had to depict proteins as schematics in papers, u know the dilemma: how to create a pretty “blob”? If structure ends up big & central, u’ll render it with PyMol et al, but for a truly schematic depiction u want a biorender-style icon, but for YOUR protein. Think I’ve finally found a way.

#Pseudomonas aeruginosa destroys other #bacteria using #T6SS and its effectors during infection in #cysticfibrosis patients. Here, we determined the #cryo-EM #structure of the effector Ptx2, which depolarizes target bacterial membranes. Check it out! www.biorxiv.org/content/10.1...

Structure of the F-tractin-F-actin complex. A new study by Dmitry Shatskiy, Athul Sivan, Roland Wedlich-Söldner @uni-muenster.de, and Alexander Belyy @alexanderbelyy.bsky.social @unigroningen.bsky.social: https://buff.ly/3EIqAur #Cytoskeleton #StructuralBiology #Biochemistry #Actin

New: Using F-tractin to visualize #actin in living cells? Dmitry Shatskiy, Athul Sivan, Roland Wedlich-Söldner, and Alexander Belyy @alexanderbelyy.bsky.social determined the F-tractin-F-actin structure and showed that this probe is nearly identical to #Lifeact. https://buff.ly/3EIqAur #cytoskeleton

Are you doing cell #biology? Then, let me guess, you likely stained #actin! Our @jcb.org article reveals the structure of the popular actin probe F-tractin in complex with F-actin and introduces an optimized version for better imaging! A thread! 👀👇 rupress.org/jcb/article/...

If you think you know everything about enzymes, check this out, we have a surprise for you 😃