Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification www.biorxiv.org/content/10.1101/2025.09.14.676144v1 #cryoem

Correlative MS Imaging for cellular identification and analysis of in situ cryo-ET www.biorxiv.org/content/10.1101/2025.09.16.676641v1 #cryoem

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

Improving cryo-EM maps by resolution-dependent and heterogeneity-aware deep learning www.biorxiv.org/content/10.1101/2025.09.03.674102v1 #cryoem

Genetically encoded FerriTag as a specific label for cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40925364/ #cryoem

Dear scientific community, I am looking for two postdoctoral structural biologists to join my group at the Department of Biochemistry, University of Oxford. my.corehr.com/pls/uoxrecru... my.corehr.com/pls/uoxrecru...

Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️

Cryo-focused ion beam milling for cryo-electron tomography: Shaping the future of in situ structural biology pubmed.ncbi.nlm.nih.gov/40848707/ #cryoem

Automated removal of corrupted tilts in cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40741136/ #cryoem

Systematic Characterization of Optical Aberrations Reveals Cryo-FLM Localization Fidelity pubmed.ncbi.nlm.nih.gov/40766645/ #cryoem

Cryo-EM protein structure without purification www.biorxiv.org/content/10.1101/2025.08.13.669967v1 #cryoem

We’re hosting the @embo.org Practical Course on In-situ structural biology by CryoFIB and CryoET in April 2026! 🧬❄️ You can expect hands-on training, cutting-edge methods, and a chance to work alongside leading experts in structural biology. 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...

Amazing structural biology unveiling mechanisms underlying mRNA transport and localization (and a tribute to the pioneering work in Drosophila that discovered these proteins through effects on embryo and ovary patterning!)

Di-Gembodies (dimerized nanobodies) for improved and duplexed CryoEM of small and challenging targets! A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social Out now in Nature Chemical Biology @natchembio.nature.com www.nature.com/articles/s41...

Very pleased to have our new work out where we perform a structure/function study on the bornavirus polymerase complex with fantastic coauthors. Have a read www.nature.com/articles/s41... @warwicklifesci.bsky.social @loiccarrique.bsky.social @franziskaguenl.bsky.social

A two-step mechanism for RIG-I activation by influenza virus mvRNAs www.science.org/doi/10.1126/...

Affinity-ligand purification of native human low-abundance multi-protein complexes for structure determination www.biorxiv.org/content/10.1101/2025.07.31.667873v1 #cryoem

🧬Latent-X is our first frontier model for protein design. For mini-binders, our AI model achieved picomolar binding affinities — the strongest reported vs prior methods in head-to-head lab validation. Explore our best binders here: platform.latentlabs.com More details on the breakthrough in this🧵

🚨 New EMBO Practical Course! Ultrastructure Expansion Microscopy: From Cells to Tissue 🔬 📅 20–24 Apr 2026 | 📍 EMBL Heidelberg Co-organised with @banterlegroup.bsky.social @gautamdey.bsky.social 💡 Register your interest to get notified when registration opens: 🔗 www.embl.org/about/info/c...

And here it is now in print! www.cell.com/cell-reports... With a front cover splash 😍

Hello everyone! I am pleased to share information on the first ever Computational Structural Virology Symposium, conducted August 4th on zoom and highlighting work in this emerging field. You can register for this event here: forms.gle/CNiqskMwQEuV.... Please re-post!

HIV-1 nuclear import is selective and depends on both capsid elasticity and nuclear pore adaptability pubmed.ncbi.nlm.nih.gov/40624219/ #cryoem

Illuminating druggable dark matter in RNA virus replication using in situ cryo-EM pubmed.ncbi.nlm.nih.gov/40639319/ #cryoem

🚨 New preprint! Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM www.biorxiv.org/content/10.1...

“This is one of the most exciting recent advances for influenza prevention." www.science.org/content/arti...

This paper combines two sort-of-crazy (in the best sense) approaches in cryoEM sample preparation: (1) Native, soft-landing electrospray ion beam deposition, followed by (2) deposition of amorphous ice, and reheating and freezing by laser flash melting. Has potential for time-resolved studies.

Excited to present the first review from my lab! Which mutations allow H5 influenza to jump into humans or other mammals? How close is H5 to a pandemic? This review has the answers and explains the molecular mechanism behind the mutations. Led by @fcapelastegui.bsky.social. doi.org/10.1099/jgv....

🚨🔎 Out now in PLOS Pathogens ! 🔎🚨 This project has taken us @cvrinfo.bsky.social @uofgmvls.bsky.social on a wild and wonderful journey. I am excited to share our story of how influenza A viruses (IAVs) exploit cell death to facilitate its covert spread. N/1 journals.plos.org/plospathogen...

New method toward modeling cells and viruses! HMFF enables integrative membrane modeling and simulation guided by electron microscopy data. Created by Valentin Maurer and Marc Siggel @embl.org ‪‪@cssbhamburg.bsky.social‬, in collaboration with @WPezeshkian lab (1/n) www.biorxiv.org/content/10.1...

If u ever had to depict proteins as schematics in papers, u know the dilemma: how to create a pretty “blob”? If structure ends up big & central, u’ll render it with PyMol et al, but for a truly schematic depiction u want a biorender-style icon, but for YOUR protein. Think I’ve finally found a way.

Really excited to share our resource and analysis of AlphaFold-predicted monomers and homodimers across viruses and phages. Project driven by fab PhD student @rodai.bsky.social and with our great collaborators Joana Pereira @joanampereira.bsky.social and @ninjani.bsky.social

@structurabio.bsky.social is killing it with these SPA case studies. This one on dealing with preferred orientation is particularly good IMO (as it is a frequently encountered issue)

Excited to share our new @science.org paper! Led by postdocs Ruchao Peng and Xin Xu, we used cryo-EM/ET to reveal the influenza ribonucleoprotein complex structure and its strand-sliding mechanism for RNA synthesis, paving the way for new antivirals. www.science.org/doi/10.1126/...

Preprint from the lab🚨 Have you ever engineered proteins to be more stable and were unhappy about your predictor's success rate? We got you covered with BoostMut! Great work led by @kerlenkorbeld.bsky.social now online at www.biorxiv.org/content/10.1... A thread 🧵

Exciting job opportunities! Two postdoc positions are available in the Fodor Lab at @dunnschool.bsky.social to study influenza virus transcription, genome replication, nuclear export and assembly. More info: my.corehr.com/pls/uoxrecru...

We are currently seeking an ambitious, motivated, and hard-working scientist to join my lab (wrobel-lab.org) at the Department of Biochemistry of the University of Oxford as a Postdoctoral Research Associate. If interested, please go here directly: tinyurl.com/postdoc-wrob... or read more below.

You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software! Hope it will be useful !

Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social, @marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social 🔗 Preprint here tinyurl.com/3a74uanv

Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...

#CryoSPARC v4.7 is out! 🚀  Featuring a new automatic Micrograph Junk Detector that labels and rejects contaminants, new tools for curating subsets of particles, performance and stability fixes, and more! Full changelog: cryosparc.com/updates #cryoEM

Advances, challenges, and applications of cryo-electron tomography workflows for three-dimensional cellular imaging of infectious pathogens pubmed.ncbi.nlm.nih.gov/40165665/ #cryoem

#NSV2025 Two weeks left to register! The abstract submission/registration system on the website will CLOSE Friday, April 11, 2025. Visit www.negativestrandvirus.org/home

Very excited to share a new paper from the lab! We visualize RSV infection with single-vRNP resolution and show how the virus coordinates transcription and replication with the formation of viral factories, biomolecular condensates that act as sites of viral genome control. doi.org/10.1101/2025...

Molecule-MCP is a very cool tool connecting PyMOL and ChimeraX directly to Claude through the Model Context Protocol for prompt-assisted molecule modelling. Great stuff by Jinyuan Sun et al github.com/ChatMol/mole...

We are thrilled to announce the 2025 EMBO practical course in cryo-em image processing, Birkbeck College, London, 9-16 Sept 2025. More info & apply: meetings.embo.org/event/25-cry... Organisers Giulia Zanetti & Helen Saibil. Beautiful image from co-organizer @carolynmoores1.bsky.social lab.

AreTomoLive: Automated reconstruction of comprehensively-corrected and denoised cryo-electron tomograms in real-time and at high throughput www.biorxiv.org/content/10.1101/2025.03.11.642690v1 #cryoem

1/ Transposable elements are often called "jumping genes" because they mobilize within genomes. 🧬 But did you know they can also jump 𝘣𝘦𝘵𝘸𝘦𝘦𝘯 cells? 🤯 Our new study reveals how retrotransposons invade the germline directly from somatic cells. www.biorxiv.org/content/10.1... A short thread 🧵👇

Automated Removal of Corrupted Tilts in Cryo-Electron Tomography https://www.biorxiv.org/content/10.1101/2025.03.13.642992v1

Structural biology is in an era of dynamics & assemblies but turning raw experimental data into atomic models at scale remains challenging. @minhuanli.bsky.social and I present ROCKET🚀: an AlphaFold augmentation that integrates crystallographic and cryoEM/ET data with room for more! 1/14.

Interested in learning to process cryo-ET data in the latest version of Warp? I'll be running a workshop on April 22nd in central London, UK details+registration: forms.gle/FLDr3ZWtVGXs... Hope to see you there!