Until we recognise that resilience requires inefficiency – that robust systems must have slack – we’re just optimising our way towards collapse. www.theguardian.com/commentisfre...

Need a better way to purify your favorite plant proteins? 🎣 Check out the Catch & Release toolbox! Find plasmids here: www.addgene.org/browse/artic... Thanks @kunzlab.bsky.social for sharing!

Cool work from our neighbors at @cultivarium.bsky.social using components from the POSSUM Toolkit! What will they (or you!) find next? Check out the toolkit here: www.addgene.org/kits/cultiva...

We’re looking forward to #SfN25! 🧠 Tell us about your research using materials available through Addgene, so we can celebrate your science! Learn more: https://twp.ai/4it0di#SfN25!

Thanks for sharing @piatkevich.bsky.social! 💡 These red calcium indicators and more are featured in our Fluorescent Protein Biosensors Collection: www.addgene.org/fluorescent-...

Does anyone know of good antibodies to tdTomato and GFP that they are happy with for ultrastructure or glut fixed tissues? #SciSky 🧪

small SOS: My lab is struggling to use Napari to visualize and work with MERFISH images. Can one of you kind experts give my student a few pointers? @mads100tist.bsky.social

Fresh batch of Hot Plasmids here! Find new tools for: 🧠 Neural cell-surface proteomics 🔋 Nerve-to-cancer mitochondrial transfer @greletlab.bsky.social 🔬 Red calcium biosensors @massecklab.bsky.social & @piatkevich.bsky.social ✂️ CRISPRa-AAV for adipocytes @nadavahituv.bsky.social & more!

Additionally, applications are now open to join the 2026 Cohort of PREreview Champions. Learn more about what to expect and apply today: content.prereview.org/2026-prerevi... Please consider sharing this opportunity with others in your community! Thank you!

We wrote the Strain on scientific publishing to highlight the problems of time & trust. With a fantastic group of co-authors, we present The Drain of Scientific Publishing: a 🧵 1/n Drain: arxiv.org/abs/2511.04820 Strain: direct.mit.edu/qss/article/... Oligopoly: direct.mit.edu/qss/article/...

Are you presenting research at #SfN25 that uses materials available through Addgene? Join our Addgene Ambassadors program! Learn more: https://twp.ai/4ispwV

Newly released Stockholm Declaration recommends the following reforms to publishing: 1. Academia resumes control of publishing 2. Incentive systems to merit quality, not quantity 3. Independent fraud detection and prevention 4. Legislation and policies to protect science quality and integrity

🕰️In November 2000, Nikon launched MicroscopyU, an online educational hub aimed at inspiring microscope users worldwide. 🌐MicroscopyU continues to be a trusted source of learning and inspiration. 🔗Explore tutorials, techniques & insights: microscopyu.com #NikonMicroscopy

"Science is being destroyed across many agencies" say federal researchers. For my latest @nature.com piece, I spoke to 19 different scientists across EPA, NOAA, NIH, NASA, and USGS to document how science is being dismantled across US federal agencies.

A small 🧵 encouraging people to publish protocols. Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work. www.biorxiv.org/content/10.1...

To be clear, it's a very particular type of junk article (possibly completely fake), not "40% of all stroke research". But yeah, if you're doing a lit search on your gene of interest and come across papers that look like these, just say nope 🙅

Anyone have book recommendations for graduate students for scientific writing and/or presenting ?

Thinking we should make some monoclonal antibodies so wondering what experiences people have had with different companies, as it's not going to be cheap so want to get our moneys worth?

If you use PomBase, please help us to demonstrate our value, and make improvements by completing our user survey www.surveymonkey.com/r/7GXJL6X

Animals don't send emails. Animals don't post rage bait on social media. Vampire bats feed their friends. Humpback whales make dinner together. We can learn so much from animals. Learn a new lesson from animals every month in the Love Notes from Nature 2026 calendar! Get one at squidfacts.net

I love this series from @thetransmitter.bsky.social, and also remember having such a strong impression from reading this paper in grad school. Even tiny parts of the cell are wildly crowded and complex! www.cell.com/cell/fulltex...

Very happy to share an expansion to the comparative gene editing toolkit: AAVs to reduce dopamine receptor levels that are functional in many rodent species. Fun collaboration with @malu-murugan.bsky.social, Elliott Albers and Frank Meye.

Bluesky, we need your help! Why does plain eGFP partially colocalize with actin filaments in cells? (No bleed-through, observed in multiple cell lines. Whole plasmid sequenced.) #FluorescenceFriday #Microscopy

Developers, it’s live 👇 The Addgene Developers Portal gives you API access to plasmid + sequence data. Query, integrate, and build with Addgene’s plasmid data. Docs + access:

🧠🥼🧬🧪🔬 Last year at @sfn.org I ran a workshop on "Opening the File Drawer" with Ben Kolber, Stacey Winham, Paul Sternberg, & Alex Freeman (@alexandrafreeman.bsky.social). We've shared the resources that we compiled for that workshop in this new paper. ❤️ @comm4rigor.bsky.social @ummni.bsky.social

Thanks for sharing your tools! 🧬🧑‍🔬 Find the plasmids here: www.addgene.org/browse/artic...

🧪Hello friends! I'm working on a piece about putting together the best tenure/promotion package, and I'd love to hear from STEM folks who have recently done this or have experience evaluating these types of proposals. Please RT for reach, and feel free to reach out with any Qs!

We love sharing tools developed by @hhmijanelia.bsky.social GENIE Project! 🧠🔬🧬 Find their plasmids with biosensors for calcium, GABA, glutamate, and more: www.addgene.org/GENIE_Project/

🧞Like its namesake, Janelia’s GENIE Project Team makes wishes come true. Luckily for biologists, this genie doesn’t grant just 3 requests. 🧪Learn about how GENIE has accelerated the development of best-in-class sensors for measuring neuronal activity. https://hhmi.news/4gFqwKX

Query from Slack: Looking for people with experience in proximity labelling in zebrafish? Considering using miniTurbo instead of BioID or APEX, what do the community think of it? Also looking for anybody that has experience with targeting labeling enzymes to the ER/secretory pathway? #AskZebrafish

Chonnettia Jones, Addgene’s President & Executive Director, is one of Endpoints News’ 2025 Top Women in Biopharma R&D! She is driving Addgene’s growth to enable more discovery and innovation, powered by the sharing of scientific resources. 🔗

New CRISPR libraries from the @johndoench.bsky.social and Root labs — Jacquere (human) and Julianna (mouse). A perfect pairing for your next screen. 🍷🧀 https://twp.ai/9PX2kT https://twp.ai/4irnYP

There’s some things I disagree with here but this is absolutely on point: “Science moves faster when we write for each other rather than for gatekeepers...The transformation of scientific communication requires scientists willing to experiment and builders ready to support them.”

🧪New from me for @nature.com: Nearly every model organism used in research has it's own database, called a MOD. These resources underpin decades of discovery, but they're collectively struggling amid funding cuts. www.nature.com/articles/d41...

These plasmids from @promegacorporation.bsky.social are exceptional new offerings from Addgene for a few reasons: - Since there is no MTA, they are available to industry customers (joining an ever-growing catalog subset: www.addgene.org/industry/) - They are available in a DNA format!

New AAV tools from @e11bio.bsky.social! PRISM uses protein-tag barcodes for high-throughput reconstruction of neurons and other cell-labeling applications. https://twp.ai/4iruEB

Identification of optimal fluorophores for use in the Drosophila embryo by Timothy E Saunders and team: www.biorxiv.org/content/10.1...

⚠️ Half of commercial antibodies miss their target! So how do you pick the right one for your experiment? Here’s the solution 👉 Antibody characterization data (all tested in KO cells) are now available at: 🔗 onlygoodantibodies.co.uk Tested by @ycharos.bsky.social & @oga-community.bsky.social

"the benefits of NIH research in general are wide and diffuse. A very large number of advances—roughly one in two during the 21st century—are linked to NIH grants that are cut in our counterfactual scenario."

This paper does a great job with a "It's a Wonderful Life" scenario about NIH, supposing the consequences of the bottom 40% of the funding NIH grants never existed. www.science.org/doi/10.1126/... tl:dr The world would lose a lot, but directly and indirectly

Frequent user of epitope tags? Try ours! IPI epitope tag antibodies & plasmids are now available through our partner @addgene.bsky.social! buff.ly/YEuZDIp

preLights is a #preprint highlighting service—supported by @biologists.bsky.social—encouraging #ECRs to highlight and discuss preprints that spark interest or debate within the biological #community. #SciComm Interested in joining? Our 'call for preLighters' is open for another week! ⬇️👀

The expanded Promega Collection is here! 250+ new plasmids for cancer research, protein interactions, targeted degradation & more. ✔ Industry + academic access ✔ No MTA required ✔ Aliquots or stabs to fit your workflow Check it out: https://twp.ai/4iqKEV

My only regret is that we didn't go with the title "One buffer to rule them all, and in the darkness bind them", although "Squeaky clean staining" was a pretty good options too!

Do you know what happens when you touch a carnivorous sundew plant? If the touch is strong and large enough, a cytosolic calcium wave will spread from the site of touch throughout the whole plant, but if you only touch one tentacle (see post below), the calcium wave will be local and less intense.

New at Addgene! Three new yeast toolkits are now available for the community. Details in thread ⬇️ https://twp.ai/4iqKvQ

Development does not happen in isolation! Material properties, geometry, stiffness, fluid flows...can all steer how development unfolds. If you’re exploring these kinds of questions, we’d love to see your work @dev-journal.bsky.social More info below ⬇️

It's #FluorescenceFriday! Another movie from our preprint, showing exocytosis of VAMP2-pHluorin (live-cell TIRF, blue) correlated with 2-color STORM of spectrins (green) and clathrin-coated pits (magenta). www.biorxiv.org/content/10.1...

👀"robust treadmilling requires an optimal phase separation strength to maintain high local concentration while preserving condensate fluidity. A weakened phase separation fails to stabilize the bundles, whereas overly cohesive condensates impede filament dynamics." #actin #phaseseparation