AAV9 is coming soon to our Packaged on Request options! Known for its broad tropism and widely used in neuroscience + gene therapy, AAV9 gives you more flexibility to design and scale experiments. Be the first to know when it’s live — sign up for Product & Service Updates: https://twp.ai/4iw7aJ

🔵mTurquoise2-based glucose biosensors TINGL and THINGL from @joachimgoedhart.bsky.social and the Teusink Lab! Find the plasmids here: www.addgene.org/browse/artic...

Scientist's way of saying 'I ❤️ you' "I found this paper/preprint that made me think of your project." "I checked the mice/cells/plates already." "I just sent you the edits you requested." "I have a slide/diagram that will work for that." "I had that problem with [lab equipment], I have a fix."

❤️ Happy Love Data Week! #LoveData26 Coming up 2/11: Improving Data Sharing w/ GREI’s Submission Checklist Learn to plan, prepare, & publish data in a generalist repository. This webinar shares steps to more FAIR data, ft. examples from NIH-funded researchers. Register:

Been on multiple search committees For me (personally so YMMV)…I basically dismiss everything that’s labeled *submitted* unless there’s a preprint I can read

Using ampicillin as a selectable marker? You can skip the post-transformation outgrowth! Why? Because Amp targets cell wall synthesis, not protein synthesis, so the bacteria isn’t impeded from making the protein it needs to combat it! youtu.be/XZn8QG6dzgc

So many developers have sent me that Anthropic skills/mastery case study that I realized I should ungate what I *already wrote* about this: beginning principles to design workflows that work *with* your mind, not against it, & protect your problem-solving www.fightforthehuman.com/cognitive-he...

Today, I would like to honor the memory of Roger Y. Tsien, born on February 1, 1952. His legacy lives with all who use his technologies, including calcium sensors, fluorescent proteins, the acetoxymethyl (AM) ester, & many more! #FluorescenceFriday www.nature.com/articles/nme...

I had intended to post something about this new Google DeepMind paper that appeared yesterday in Nature, but the press coverage has added to what there is to say. So this is a long 🧵 www.nature.com/articles/s41...

Make your gene knockouts more efficient with multiplexed Cas12a sgRNAs. Our new paper is out now, with tools available from @addgene.bsky.social , www.plasmids.eu and @vdrc-flies.bsky.social. www.nature.com/articles/s41... #CRISPR #geneediting #Drosophila 🧪🧬✂️🔬🪰 Summary 🧵 below.

A few months at nursery gives a roughly 1-year-old baby more new microbes for its gut microbiome than it has acquired in its WHOLE LIFE up until that point from its family. 🤯 🧪 #microbiome #science My latest for @nature.com www.nature.com/articles/d41...

Great news! @mariaingaramo.bsky.social 's company (Nonfiction Labs) made a remote-controlled antibody. Its binding turns on and off with a magnet. This is a HUGE step towards our dream of magnetically controlled drugs. Imagine a cancer drug that ONLY attacks the tumor, not the rest of your body.

Help requested: my friend is interviewing for TTF positions at private SLACs in the US, and wants to prepare a startup package list that is fitting. Can anyone share startup $ ranges at their SLACs, so it's a reasonable number? Please RT.

This is so grim. Science isn’t just something nerds do, but the foundation of the modern world. What the Trump regime has done to US science will harm Americans for generations.

One of my concerns with AI-assisted research in biology is that we're already looking only in the lamplight and AI incentivizes us to narrow the beam. Seems like it's happening... www.nature.com/articles/s41...

Are you an industry scientist? Addgene's mission is to empower scientists with access to research materials, and that means you too! Our repository includes nearly 10,000 tools available to industry laboratories. Check them out in our latest blog post:

2) What's REPROcode? A barcoded, arrayed lentiviral library of 408 immune TFs (now available through Addgene - www.addgene.org/Filipe_Perei...) + scRNA-seq that links which reprogramming TFs a cell receives to the identity it acquires. With no extra barcode PCR steps!

It's easier to tear down than build up, and in science we have a bad case of that. So: how to lead a journal club that actually finds value in what you read. scientistseessquirre... 🧪

Preprints of pandemic potential - new historical piece from me on the history of bioRxiv/medRxiv, their role in the pandemic, and the way forward. 1/n journals.asm.org/doi/10.1128/...

🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)! An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools. 🗓️ Apply online by Jan 30, 2026

Giving the gift of negative data 🥰 Since I joined @lsajournal.org I have handled several stellar manuscripts that report null results. These papers do very well and reviewers almost always appreciate them! Thanks @rockefeller.edu news team for the article! www.rockefeller.edu/news/38829-t...

Interesting to see support for the hypothesis that the rainbow palette isn’t inherently ordered (the colors don’t map to "more" or "less"). www.sciencedirect.com/science/arti... #dataviz 📊 #color 🎨

We recently published a paper in @plosbiology.org We found a lot of problems in animal studies of hemorrhagic stroke. Research integrity folks found the paper but the neuroscience community seems to have missed it completely. I'd like them to read it too! doi.org/10.1371/journal.pbio.3003438

"We need to stop measuring what's easy to measure and start looking for what's important." - Tony Ross-Hellauer [2021] Example case study: UniProt database saves users €1000s per year in the "time they would have otherwise spent digging up the information from other sources"

A free, open-access library of high-quality organism illustrations for science communication

On delayed application review, the NIH grant terminations lawsuit appears to be headed for a settlement. Both plaintiffs and defendants have submitted a joint document outlining their agreement. Judge Young must sign off on it before it takes effect. Here's what it says and what all this means 🧵

We’ve highlighted some wonderful images and researchers in our ‘Featured image’ series in 2025. To celebrate, we’re inviting you to vote for your favourite in our image competition! #FluorescenceFriday Check them out here: focalplane.biologists.com/2025/12/19/v...

For my cytoskeleton cell biology colleagues, this preprint from Sho Ono at Emory disproves and corrects a long-standing accepted notion (one that I have accepted since 1976 when I started in the actin field). www.biorxiv.org/content/10.1...

🧵 Cytoskeleton question of the day: You want to localize your favourite protein. What do you trust first? 🔹 GFP overexpression 🔹 CRISPR knock-in 🔹 Commercial Antibodies 🔹Homemade antibodies 🔹 Or “all of the above + controls” 👀

☺️ 🧪 www.nature.com/articles/d41...

Last Friday, the @NSF released an RFI for a new Tech Labs Initiative, to "build and scale next-gen independent research organizations to advance science."

An important reminder that the wild and wonderful are often not recapitulated by model organism biology. The Rudner lab shows that the biogenesis of #anthrax spores has striking differences with the process in B. subtilis 🧪

Organelles do NOT have a single uniform pH. And if you think they must, because “protons diffuse fast,” this paper is for you. A thread on why that assumption is wrong; and what we found instead. 🧵 1/n

Attendance at US-based science conferences is bouncing around this year but generally down. #AGU25 has ~10k fewer this year! I explored these trends for @nature.com — including solutions to keep conferences local & productive: www.nature.com/articles/d41... 🧪

Getting SMART about FRAP enabled us to make many FRAP curves in one imaging session. Now we are doing things like 18 conditions n = 30 in a single overnight session. Study protein dynamics in high throughput. Curious on how? see our publication: febs.onlinelibrary.wiley.com/doi/full/10....

Pleased to share this thoughtful essay by Jess on my flower design project🌹🧬 As a small thank-you for spreading the work, I'm randomly giving away 5 of these new morphogenesis hoodies to whoever reposts this!

I just published: In praise of experts: distinguishing sense from nonsense in the age of AI Can chatbots flag bad science? In a sea of polished, plausible noise, expertise matters more than ever. medium.com/p/in-praise-...

This is excellent: Tips and Tricks for Writing Constructive Peer Reviews Perhaps the most excellent part is this subhead: "Remember that peer review is not meant to crush souls". Right? Peer review should improve papers (and OK, gatekeep a little) - not make an author regret their career choices!

🤩 We love seeing agar stabs arrive at the lab! Thanks for the explainer and great tips @bumblingbiochemist.bsky.social

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Leaders at @ukri.org should be seriously looking at the lottery first idea. 68% lower economic costs, increase in diversity of award holders and well received. www.nature.com/articles/s41...

HELP! Please suggest a cool paper/preprint in your field in the last year or so. For a course I teach, I am collecting papers on: cell/dev bio, microbio, neuro, maths/stats/CS/chem/phys applied to biology. 1/2

Microscopy Bat signal! I want to compile a list of biological samples that demonstrate microscope capabilities, including the reagents to generate them. Please send me anything you know of!! Beads are great for QC, but there's a different teaching benefit with biology. 1/n

🧠 Neurofriends: How do you decide which papers you will review? * Do you decide based on the journal? (previously published there, open science policies, impact factor, APCs?) * Are you more likely to review papers that look higher quality vs lower quality? * How many do you accept per month?

Arrows are tricky. For us, as the designer of the visual, it will be super clear what it means. But for someone looking at our visual for the first time, it can be highly ambiguous. Depending on the reader, the same arrow can mean sequence, zoom, range, label, movement,... 1/2

Applications are open for @dev-journal.bsky.social 2026 Pathway to Independence (PI) programme, supporting postdocs applying for group leader positions: Mentoring Profile raising Leadership training Network building Spread the word... www.biologists.com/grants/devel...

Science question: does your lab have a MinION sequencer and does it work well? Worth the money? (thanks for any opinions!)

Hey, what are you gonna get for the office secret Santa? For your mother in law who loves her bird feeder? For your niece who loves whales? For your dad who literally has no hobbies and you’re like “shit wtf do I get this guy”? Get a love notes from nature calendar! squidfacts.net

Let's gooo! 📣 I'm running the 2026 Boston Marathon as a fundraiser for Boston Children's Hospital! If you're looking for causes to support on #GivingTuesday, I'd be grateful for any contribution: secure.childrenshospital.org/goto/mikelac...

Ok, this one may be controversial 4) Initially, I focus on understanding what the authors are saying, not trying to critique the science, that comes later. **Often I don't even look at the figures unless the point is not clear from the text.** 5/🧵