Plasmid errors are more common than expected, and not every method catches them all. Find the right approach in our webinar with plasmidsaurus 📅 July 15 | 12 PM ET ✅ Sanger, short + long-read ✅ Interpret quality metrics ✅ Pick the right approach Register: https://twp.ai/4htXC1

💯💯 endorse - thank you @kamounlab.bsky.social and colleagues!

“the academic community is enormously heterogeneous and it’s almost impossible to keep everyone happy” Never a truer word said in this analysis of downstream consequences of openness by @jacoates.bsky.social ripplingideas.org/2026/06/26/t...

If you’ve ever wished for hundreds of immune-specific transcription factors in barcoded lentiviral vectors, ready to use in high-throughput combinatorial screens… that’s a very specific wish. But we’ve got you, thanks to a deposit by Pereira Lab Check it out!

Heading to Barcelona for #FENS2026? Come find us at Booth 147! We'd love to chat about your research and what's new at Addgene. See you there! 🧠 https://twp.ai/4hrfwD

My first art credit! 💅 Art director Samuel Velasco and I wanted a new visual metaphor for the genome — not a code or a sequence, but its tangled, twisted physical form. More cool yarn sculptures inside! Also it's a great piece by @philipcball.bsky.social that will change the way you think about DNA

PHP.eB is now available via Addgene's Packaged on Request service. Not sure if it's the right capsid for your experiment? Our Viral Vectors 101 post on systemic capsids includes a decision tree and comparison table to help you choose.

This free webinar from my colleagues is tomorrow! Get an overview and intro to using #CRISPR in the lab - great for students or anybody new to the topic 🧬🧪💻 June 18, 12pm ET / 9am PT

Excited to share our first red ACh sensor! 🔴 We developed GRAB_rACh1h, the first genetically encoded red fluorescent sensor for recording acetylcholine dynamics in vivo. (1/3)

😎 to have this out! --> stay tuned, this is a series about 📊#dataviz for #science 🧪

🧪New from me for @nature.com: I do a lot of writing about technological innovation, but sometimes, the simpler tool really is best. For this story, I asked field and bench scientists to tell me about their favorite, most basic tools! www.nature.com/articles/d41...

Neuroscientists, we’ll see you in Barcelona! 🧠Addgene is heading to #FENS2026 (July 6-10). Stop by Booth 147 during the expo (July 7-10) to chat about how we can support your research with plasmids, viral vectors, and more. Plus, enter our raffle for a chance to win Addgene swag! 🎉

At CRISPR 101 on June 18, 2026 you'll: ✅ Learn how CRISPR/Cas9 works ✅ Compare common CRISPR systems ✅ Identify the right system for your research Free, live, and presented by Addgene's own application scientists. Register today:

The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes: 🖌️ Dozens more images with duplications or painting 🖨️ Hundreds of blots that all share the same background (behold slideshow below)

Has anyone observed mNeonGreen getting brighter after photobleaching?

PHP.eB — an AAV9 derivative known for efficient, brain-wide transduction via systemic delivery — is now available through Addgene's Packaged on Request service. What cargo do you need packaged? https://twp.ai/4hqr5l

Today I'm posting a new blog about an astonishing scientific own goal. Hundreds of papers have reported using a totally wrong antibody to investigate the tumor suppressor p16. This mistake has happened because scientists have muddled the names of two proteins. forbetterscience.com/2026/06/02/m...

In the latest issue of @jcellsci.bsky.social, with @lankylaste.bsky.social, Lina Gerontogianni and Gavin Kelly at @crick.ac.uk, a reanalysis of images from the IDR to answer the age-old question: How do you tell statistical significance from biological significance? doi.org/10.1242/jcs....

TL;DR: We've identified more than 100 cases of apparent manipulation in Thermo Fisher Scientific's antibody verification data. @sholtodavid.bsky.social @johanduchene.bsky.social reeserichardson.blog/2026/05/28/h...

Two popular CRISPR KO libraries just got easier to use! Jacquere and Julianna from the John Doench and Root labs are now available as ready-to-use lentiviral preps at Addgene. Human KO: https://twp.ai/9OVfvi Mouse KO: https://twp.ai/4hqQje

The use of antibodies in science is robust, but notoriously complicated due to a myriad of factors related to storage and implementation. This… this does not help. Do better Thermo Fisher.

New plasmids from Kai Johnsson now online! 💡 The self-labeling CLIP-tag2 achieves almost 1000-fold faster tagging than the original when combined with a new substrate optimized for live-cell imaging. https://twp.ai/4hrKYy

Scientists shared their standout days for this @nature.com feature. Thx to @nicoleackermans.com @bwjones.bsky.social @aishikghosh.bsky.social @peixotoraquel.bsky.social & others for their stories! If you have a story, please send it my way for part 2!

I’m not sure if I ever shared on Bluesky this article we wrote on how almost everyone giving career advice is susceptible to survivorship bias, but I think it was good and you should read it www.nature.com/articles/d41...

Free and open-source images, icons, and tools for creating scientific illustrations doi.org/10.59350/5zt... 🧪

MagLOV 2 plasmid is (finally!) available on Addgene 🎉🧲🧫 Any GFP capable imaging setup + benchtop power supply + off the shelf electromagnet is all you need to get started - let us know if you need help of course. www.addgene.org/252303/ @andrewgyork.bsky.social @mariaingaramo.bsky.social 🧪

The accompanying blogposts are really interesting too, showing that AI usage has led to more manuscripts that are weaker with poorer writing quality. On the review side, AI peer reviews focus more on a narrow subset of theoretical issues and less on thr "empirical foundations of the work".

"this research is not costless. It is imposing a burden on the volunteer labor of reviewers and editors that holds the peer review system together." orgsci.substack.com/p/more-versu...

PinkyCaMP is finally published!! My first paper co-first authored with @sikmys.bsky.social where we make a great optogenetic and multiplex compatible red calcium indicator. Thank you to @massecklab.bsky.social and @campbell-lab.bsky.social for guiding this journey www.nature.com/articles/s41...

🧪Thrilled to see this work out in @Nature Methods from Verkhusha Lab with Natalia Barykina as 1st author @einsteinmededu.bsky.social VIS–Fbs is a multicolor fluorescent nanobody platform for imaging, and #Zebrafish helped extend it into an in vivo vertebrate system. Verry happy to be part of it 🤗

New STAT story about the chances of receiving NIH funding www.statnews.com/2026/04/30/n... 1/3

Super cool, can't wait to see what folks do with this next! Lots of good detail in the 🧵 www.addgene.org/247065/

How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!

"[They] were right,” she says. “We should have been speaking up all along." Continuously disappointed by the rare few who share rooms and prestige with the assholes running power plays to destroy science and our country. www.nature.com/articles/d41... The time to speak up for our values is ALWAYS.

Excited to share our discovery of a new programmable RNA-guided DNA-targeting system hiding inside bacteriophages that predates CRISPR. We call it VIPR (Viral Interference Programmable Repeat), and it uses an entirely new logic to find its targets. Thread + link below.

Look at this crazy thing

Excited to share our new study on how the brain generates placebo pain relief! We developed a mouse model that recapitulates key features of human placebo analgesia, then used it to identify causal circuitry. www.sciencedirect.com/science/arti... today.ucsd.edu/story/neurob... 1/n

Check it out: addgene.org has a new look! 🧬✨ I've been working here almost 5 years and it's so exciting to see how much the organization has grown in our 20+ years. The mission is the same, but now evolved to continue powering scientific sharing into the future.

"The selfish ribosome" paper is now published in @plosbiology.org . Have a look! Many thanks to the editors and reviewers! doi.org/10.1371/jour...

Chasing donor backbone insertions in #CRISPR knock-in experiments is a great way to waste an afternoon (or more), unless you use a dominant marker. We like this one from the Carthew lab, in which animals containing the backbone have degenerated eyes: www.tandfonline.com/doi/full/10.... #Drosophila

Ran my second Boston Marathon yesterday! A day of ups and downs - all for the kids at Boston Children's Hospital 💙 If you like, you can donate here: secure.childrenshospital.org/goto/mikelac...

But if you're an editor and handle clinical AI, becoming a friend with the TRIPOD AI checklist may not be the worst idea 14/ www.equator-network.org/reporting-gu...

We are looking for a human MALAT1 overexpression plasmid. Does any of you kind people have it?

“I’m not saying all this to defend humanity. Humanity sucks. It’s totally terrible. I’m saying this because I believe in an old-fashioned virtue called Doing the Freakin’ Work.” www.nytimes.com/2026/04/15/o...

SynORI plasmids from the Chappell Lab solve problems with plasmid copy number and plasmid incompatibility. If you want to modify your plasmid instead of your experiment, check out their post about the tool here:

Mirror mirror on the wall who is the brightest of them all? Brightness is a key parameter for choosing fluorescent reporters in bioimaging. As tricky to assess, we present a pipeline to compare chemigenetic labels and FPs in tissue culture and #zebrafish embryos. www.biorxiv.org/content/10.6...

This is so, so well-articulated.

Congrats to Dennis Botman - the driving force behind this study - for this publication: Side-by-Side Systematic Characterization of Novel FPs in Budding Yeast pubs.acs.org/doi/10.1021/...

Thank you @biologists.bsky.social for sharing these numbers. "quality editorial assessment and publishing involves a lot of people [salaries & editor stipends] ... and publishers have had to invest heavily in technologies to support professional and trustworthy online publishing" ...

A thought about job adverts. I assume seeking "exceptional" individuals selects for specific character traits (eg self confidence). My goal is to get candidate with best potential, even if they don't yet think of themselves that way. What words do you use to encourage the widest pool of applicants?