Find the hot-off-the-press plasmids here! www.addgene.org/browse/artic...

Hot Plasmids are here! We've got photostable mScarlet3 variants from @zhifei-fu0326.bsky.social and Pingyong Xu labs, improved genome-wide CRISPR knockout libraries from John Doench and David Root, the latest antibodies from @ipiproteins.bsky.social, and more:

AAV9 is coming soon to our Packaged on Request options! Known for its broad tropism and widely used in neuroscience + gene therapy, AAV9 gives you more flexibility to design and scale experiments. Be the first to know when it’s live — sign up for Product & Service Updates: https://twp.ai/4iwER5

Another round of Hot Plasmids, coming right up! Read the blog to learn about our new-and-improved AAV packaging plasmids, a cellular time capsule, E. coli recoded to use a single stop codon, and more!

Our #CellLineOfTheWeek is Tom20 - illuminating the powerhouse of the cell! 🧫 Our stem cell lines & plasmids are available for just the cost of shipping to reduce the barriers to scientific discovery. 📦 Distributed by @coriellinstitute.bsky.social and @addgene.bsky.social#CellLineOfTheWeek #OpenScience

AAV9 is coming soon to our Packaged on Request options! Known for its broad tropism and widely used in neuroscience + gene therapy, AAV9 gives you more flexibility to design and scale experiments. Be the first to know when it’s live — sign up for Product & Service Updates: https://twp.ai/4iw7aJ

🔵mTurquoise2-based glucose biosensors TINGL and THINGL from @joachimgoedhart.bsky.social and the Teusink Lab! Find the plasmids here: www.addgene.org/browse/artic...

We learned about hybrid RNA-DNA guides for CRISPR applications at last year's American Society of Human Genetics meeting. Now we're bringing you the overview: applications, advantages, and caveats of this approach.

🎇PINK1-SPARK: A phase separation-based biosensor for PINK1 activity! Find the plasmids here: www.addgene.org/browse/artic...

Check it out! Plasmids for transposon mutagenesis in non-model bacteria: www.addgene.org/browse/artic... 🧬🦠 And lots more of @cultivarium.bsky.social's shared tools: www.addgene.org/Nili_Ostrov/

Mark your calendar! We're proud to sponsor this free webinar from @repro4everyone.bsky.social featuring @doctorzen.net on designing effective scientific posters. Don't miss this great resource for students and researchers!

Thanks @michaelboutros.bsky.social and @crisprflydesign.bsky.social for sharing! 🧪🧬✂️🔬🪰 Multiplexed Cas12a editing plasmids here: www.addgene.org/browse/artic...

New #Drosophila vectors for synchronous temporal control of independent transgenes in distinct cells or tissues 🪰🧬 Thanks @mccabelab.org for sharing! www.addgene.org/browse/artic...

We’re excited to welcome Daniela Bourges-Waldegg to the SeqHub Advisory Board! Daniela is EVP + Chief Digital & Technology Officer at @addgene.bsky.social. She will help shape our approach to building researcher-centered digital infrastructure with an eye toward long-term scientific impact.

PI4P probes: Protocol, paper, and plasmids! Thanks for sharing your tools and expertise @shinyatsukiji.bsky.social 🧬 Get the plasmids here: www.addgene.org/browse/artic...

Are you an industry scientist? Addgene's mission is to empower scientists with access to research materials, and that means you too! Our repository includes nearly 10,000 tools available to industry laboratories. Check them out in our latest blog post:

Addgenies are excited to be at #AUTM this week, connecting with technology transfer professionals from around the world 🌍 Don’t miss the session, "Industry/Academia Connect & Collaborate – Part 1," where Addgene Co-founder & Chief Innovation Officer Melina Fan, PhD, will present.

The same priorities we’re focusing on at Addgene — efficiency and quality — are shaping biopharma R&D. A new #ScripAsks looks at what’s next, with Chonnettia Jones noting that 2026 will push doing more with less through CROs and AI-enabled workflows.

Collaboration drives scientific progress. In our latest blog, Chonnettia Jones reflects on how Addgene strengthened its foundations in 2025 and where we’re headed next to support the global research community. https://twp.ai/4iumZP

Planning to be in Seattle for the AUTM Annual Meeting on Feb. 8–11? Us too! Don’t miss the session, "Industry/Academia Connect & Collaborate – Part 1," where Addgene Co-founder & Chief Innovation Officer Melina Fan, PhD, will present. Stop by Room 606 on Feb. 9 at 1:45pm PT. https://twp.ai/4iuaQI

CRISPR is an RNA-guided gene editing tool. Except sometimes, a bit of DNA can help guide too! Substituting a few gRNA bases for DNA decreased off-target outcomes for several different CRISPR applications. Our new blog post on the topic has the details:

PUFFFIN: an ultra-bright, customisable, single-plasmid system for labelling cell neighbourhoods. www.addgene.org/browse/artic...

Unveiling an upcoming R4E webinar focused on crafting impactful posters! Find more information here: www.repro4everyone.org/blog/r4e-web... Thank-you to @addgene.bsky.social for sponsorship! #AcademicSky #EduSky #neuroskyence

An Omp knockout strain developed by Jack Leo’s lab for improved outer membrane protein expression has been requested 100+ times — thanks for sharing! www.addgene.org/102270/

New antibody release! Explore axon guidance with confidence using the latest IPI antibodies for Netrin, DCC, and IGDCC3 (PUNC) — now available at Addgene. High-quality tools to support your neuroscience research. https://twp.ai/4itjtg

Are you an industry scientist? Addgene's mission is to empower scientists with access to research materials, and that means you too! Our repository includes nearly 10,000 tools available to industry laboratories. Check them out in our latest blog post:

An incredibly useful tool made possible by shared tools, problem-solving, and the support of colleagues and funders. And it includes a nerd alert 🤓 A story near and dear to our hearts! 💙

New from @cellreprolab.bsky.social! The REPROcode library is a collection of over 400 immune-related human transcription factors in barcoded lenti vectors. Explore the set and find your favorite or mix-and-match: www.addgene.org/browse/artic...

When you need to image in deep tissues or whole animals, look beyond the rainbow! 🌈🔬 Check out our In Vivo Imaging Collection page to find plasmids for popular far-red and near-infrared fluorescent proteins: https://twp.ai/4itjtm

Happy New Year, open science community! Addgene has been facilitating the sharing of research tools since 2004, and we shipped our first plasmid to an industry laboratory in 2012. Learn how to filter your search for availability to industry in this blog post:

Find the Ting Lab's conformationally-biased variants of lipoic acid ligase here: www.addgene.org/browse/artic...

Which new CRISPR plasmids gained the most traction at Addgene this year? Connect with Elena at #Deaminet2026 to find out!

💚 Sharing makes our future as bright as these biosensors 💚

Anti-Olig1 [N149/25.1R] Depositor: James Trimmer Anti-Olig1 (Mouse) recombinant mouse monoclonal antibody https://twp.ai/4itsJ8

Find the antibodies here! 🔬🧑‍🔬 www.addgene.org/antibodies/p...

pGEL851 Depositor: Yong Zhang Expression of TadCBEd in rice cells https://twp.ai/4itsIh

pAAV_BiLAMP5e3_jGCaMP8m Depositor: Gordon Fishell Expressing jGCaMP8m driven by Lamp5 interneuron-targeting enhancer In-stock AAV preps available in AAV1; other serotypes available on demand. https://twp.ai/4ittG2

Oh where, oh where does my protein localize? Oh where, oh where can it be?🎵 Check out our Subcellular Localization Collection page, with fluorescent protein markers for organelles and cellular structures to help you find your way 🔬

🤩 We love seeing agar stabs arrive at the lab! Thanks for the explainer and great tips @bumblingbiochemist.bsky.social

pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61) Depositor: Benjamin Blencowe Purpose: Protein expression in insect cells https://twp.ai/4isUN9

pAAV_BiPVe4_eGFP Depositor: Gordon Fishell Expressing eGFP driven by chandelier cell-targeting enhancer In-stock AAV preps available in AAV1 and PHPeB; other serotypes available on demand. https://twp.ai/4isUN5

We always love new plasmids coming online! 💖 Thanks for sharing @schmittlab-ucla.bsky.social

🧠🧬 CRISPR editing in postmitotic cells like neurons is hard. But the Fellman, Conklin, @kampmann.bsky.social, @doudna-lab.bsky.social labs and colleagues @innovativegenomics.bsky.social have found strategies to make it work! Find plasmids here: https://twp.ai/4isUMv

Congratulations to @addgene.bsky.social depositors @jourdainlab.bsky.social, @mpercharde.bsky.social, and @alejofraticelli.bsky.social (and maybe some future Addgene depositors?) on this honor! www.addgene.org/Alexis_Jourd... www.addgene.org/Michelle_Per... www.addgene.org/Alejo_Rodrig...

Congrats! Thanks for sharing your tools with so many scientists around the world. Sharing speeds science!

AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA Depositor: Allen Institute for Brain Science, Jonathan Ting Nuclear SYFP2 labeling in neurons, for nuclear isolation and scRNA-seq applications In-stock AAV preps available in PHPeB; other serotypes available on demand. https://twp.ai/4isPbK

New at Addgene! Photoswitchable HaloTag, enabling robust and selective labeling of HaloTag on the surface of living cells using visible-light activation. From the @clairedeo.bsky.social lab. Walterspiel et al. Angew. Chem. Int. Ed. 2025 https://twp.ai/4isOad

Happy #FluorescenceFriday! Our #CellLineOfTheWeek is PCNA - illuminating a protein involved in DNA replication and repair! Our cell lines & plasmids are available at cost to support discovery: https://www.allencell.org/cell-catalog.html 📦 @coriellinstitute.bsky.social and @addgene.bsky.social

Thank you to the 6,700 researchers who deposit their plasmids with us — your contributions help build a more connected scientific community & unlock the full potential of research! Addgene will be closed Thurs., Nov. 27 & Fri., Nov. 28 for the Thanksgiving holiday.

pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH Depositor: Ivo Spiegel Kir2.1 expression for silencing neurons and mScarlet-KASH expression In-stock AAV preps available in AAV8; other serotypes available on demand. https://twp.ai/4itAvh