Just released! Fluorescent Protein Libraries from the Plesa Lab. Benchmarking protein expression, developing imaging tools, fluorescent protein engineering, and more. www.addgene.org/pooled-libra...

New preprint out! In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...

Join us in Amsterdam for a FEBS advanced course on "Functional Imaging of cellular dynamics" from 31st of May - 6th of June 2026 Info: microscopy2026.febsevents.org

Researchers have engineered magnetically controlled fluorescent proteins that can be remotely dimmed and brightened in cells and living animals go.nature.com/3NUSY12

In this month's Editorial, we highlight some of the Nature Methods editorial team members' favorite papers we published in 2025! www.nature.com/articles/s41...

#FluorescenceFriday: getting ready for #GEF25, playing with Ciarán's stunning ExM data

Structure, Function and Dynamics of mCoral, a pH-Responsive Engineered Variant of the mCherry Fluorescent Protein with Improved Hydrogen Peroxide Tolerance www.mdpi.com/1422-0067/27...

A reminder to use the right P2A sequence to produce separate proteins - the images are from a GPCR-P2A-mCherry construct using a P2A variant without (upper row) and with (lower row) a preceding GSG linker.

Do you like following rules… but also breaking them? So does your dopaminergic synapse! 😎🧠 Our new Trends in Neurosciences review dives into the non-classical side of dopamine signaling, notably the tools reshaping how we see it (CLEM, cryo-CLEM, serial EM + more).

How cool!!!

What an amazing opportunity to do impactful work in bioimage analysis!!!!

What would happen if cells would express more molecular motors ? They would break all microtubules 😱 As shown by recent great work from @kjverhey1.bsky.social rupress.org/jcb/article-...

This Protocol results from the Joint Undertaking for Morphological Profiling Cell Painting Consortium, and improves the Cell Painting assay by quantitatively detecting morphological phenotypes, improving the ability to group perturbations with similar behavior.

I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager! I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...

Nanotubes everywhere between neurons! A bit skeptical about these "dendrite-dendrite nanotubes" from a phalloidin staining in culture, but let's read on… www.science.org/doi/10.1126/...

Beautiful live-cell imaging of lysosome tubulation along microtubules in this work just out from @lysoluis.bsky.social et al: rupress.org/jcb/article/...

Time-resolved fluorescent proteins expand fluorescent microscopy in temporal and spectral domains. Is it impressive? Yes Is it new? No Should you use it? Probably not. www.cell.com/cell/fulltex...

Genetically Encoded Death Indicator (GEDI): a live cell biosensor protocol for high-resolution screening of therapy-resistant cancer cells by Jessica S Blackburn and team: www.biorxiv.org/content/10.1...

Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane–Membrane Contact Sites pubs.acs.org/doi/full/10....

Looking for your new favorite red fluorescent protein? mScarlet3-H (aka mYongHong) is highly stable, monomeric, and bright - it may be just what you need! https://twp.ai/4iqbT5

(Yet another) shout-out to @joachimgoedhart.bsky.social and colleagues for their ongoing technical service to the cell biology community – in this case, the yeast cell biology community. Special kudos for making all the key plasmids available on @addgene.bsky.social. Stellar work all around!

The plasmids related to our mScarlet3-H are available on Addgene now. www.addgene.org/Zhifei_Fu/

Delighted to announce here that @ritastrack.bsky.social is the new Chief editor of Nature Biomedical Engineering. Congratulations Rita!

💡 Always a good time to be reminded: "if you fuse your protein of interest to GFP or EGFP to study the protein's behavior ... you are using a tag with a serious drawback." Same goes for anything that doesn't have 'm' in its name (looking at you, dTomato and StayGold)

Nature Methods is hiring! We are seeking a new editor with expertise in microscopy, imaging technology, and/or cell biology. The position is available in NYC/Jersey City or Shanghai/Beijing on a hybrid working model. Apply by August 3! springernature.wd3.myworkdayjobs.com/SpringerNatu...

Some graduate students, postdoctoral fellows and senior researchers choose a special type of science-focused summer, for instance at @mblscience.bsky.social and CSH Asia. @vivienm.bsky.social spoke with 23 people about their experiences. Here is the story. www.nature.com/articles/s41...

Shared and not taken over by... Go for symbiosis! dx.plos.org/10.1371/jour...

Finally out in Nature Chem Bio: SNAP-tag2 for faster and brighter protein labeling www.nature.com/articles/s41... Thank you Steffi and Veselin.

Our June issue is live! www.nature.com/nmeth/volume... On the cover, mScarlet3-H, a new monomeric red fluorescent protein. Image by Zhifei Fu. Paper here: www.nature.com/articles/s41...

More nice talks at the Bioorganic GRC. Katharine Diehl tells us about pancACe (pronounced pancake) their genetically encoded acetyl-CoA sensor. www.cell.com/cell-chemica... 🥞

Spatial analysis of mitochondrial gene expression reveals dynamic translation hubs and remodeling in stress | Science Advances www.science.org/doi/10.1126/...

Great News & Views article by Ben Campbell covering recent development in FP field with focus on mScralet3-H (aka mYongHong) www.nature.com/articles/s41...

go.nature.com/42maCP9 The story of the beginning of mScarlet3-H (aka mYongHong).

A package of research papers from the MICrONS (Machine Intelligence from Cortical Networks) consortium in Nature and Nature Methods provides a detailed map of mouse brain cell structure and connections, offering insights into how they relate to activity in the mouse brain. 🧪

Thanks to my students' and collaborators' hard work, the Editor's quick responses, and the Reviewers' constructive suggestions. I was surprised at the incredible stability of mScarlet3-H, the first time I saw the results. mScarlet3-H is mYongHong. Don't be confused!