We are carrying out large-scale validation and benchmarking of AI agents for multi-omics data analysis and would like your feedback on the usage of these tools in your research (click the link for the survey) forms.office.com/Pages/Respon...

Having fun recording videos for my next lecture on Optogenetics in the Neurotechnology course at Westlake University.

Genspark helps me develop engaging opening slides and format my presentations for the undergraduate neurotechnology course I teach this semester www.genspark.ai/invite_membe...

🚀 Excited to launch the #OpenCloning Assembler! 🧬 Built to demystify Golden Gate for beginners and save time for experts -> MoClo assembly blazing fast ⚡ ✍️ Use existing syntaxes or design your own! #SynBio #GoldenGate #MoClo #OpenSource #Biotech 👉 Try it: app.opencloning.org Feedback welcome!

Wow! This is so cool and way more fun than boring BioRender! Plus, developing fine motor skills through hand drawing and creativity without limitations in shapes or form! Try it out yourself here chat.figurelabs.ai/invitation/i...

It was great pleasure hosting you at Westlake University! And you research is just mindblowing! I am looking forward to all cool technologies from your lab!

Excited to share insights on iPEX, a new technology co-developed with Yilong Zou for micrometre-resolution spatial proteomics, published in @Nature. Check out this interview to discover how tissue expansion is revolutionizing protein analysis in intact tissue! www.youtube.com/watch?v=6hKn...

Gearing up for next year’s teaching at Westlake University. Here’s my intro slide for Super-Resolution Imaging Techniques

Next-generation orange-to-far-red photoconvertible fluorescent protein for single-molecule microscopy and protein dynamic tracking www.nature.com/articles/s41...

In situ imaging proteomics via expansion (iPEX) integrates isotropic tissue magnification with MALDI mass spectrometry imaging to enable unprecedented spatial resolution and depth of protein identification for untargeted spatial proteomics www.nature.com/articles/s41...

Our red calcium indicator, FRCaMPi, and its soma-localized version, SomaFRCaMPi, are now available from @addgene.bsky.social in various vectors, along with all the sensors used for benchmarking. www.addgene.org/browse/artic...

ProteomEx is perhaps the most straightforward and simple approach for spatially resolved targeted proteomics, which requires only off-the-shelf reagents. Now with this step-by-step protocol published in Nature Protocols, anyone can do it www.nature.com/articles/s41...

AAVs are crucial vectors for delivering biosensors into neurons! Using AAV delivery, we perform two-color imaging of calcium and potassium transients in neurons. These AAVs are now available from @braincase.bsky.social www.ebraincase.com/news/new-pro...

🔬 FULL WEBINAR NOW AVAILABLE! Spatial Biology: Innovations & Applications Featuring experts: → Xiao Wang → Kiryl D. Piatkevich → Jeffrey Spraggins → Fei Chen Watch the complete session to discover cutting-edge tech 👇 live.vhall.com/v3/lives/wat...

What an incredible experience hosting a webinar on Spatial Biology: Innovations & Applications with @Science/AAAS! Missed it? Don't worry—we're dropping the full recording soon! Here's a sneak peek 👇

Great commentary by @yulonglilab on our two recent papers introducing red genetically encoded biosensors for calcium and potassium imaging! 🔴 FRCaMPi - Enhanced red calcium sensor 🔴 RGEPOs - First-ever red potassium sensors 📖 doi.org/10.1371/jour...

Spatial biology technologies are rapidly advancing, delivering unprecedented insights into cellular organization and disease pathology. Join our October 29th webinar on "Spatial Biology: Innovations & Applications" with great speakers. Register here for free en.westlake.edu.cn/academics/Sc...

High-quality AAVs for red genetically encoded calcium indicator FRCaMPi are now available from BrainCase anywhere in the world www.ebraincase.com/products-ser...

#OpenCloning opencloning.org now features WeKwikGene integration—streamlining downloading plasmid sequences from wekwikgene.wllsb.edu.cn and downstream cloning and collaborations!

Fluorescent protein-based ticker tapes (FPTT) record transcriptional histories of multiple promoters on single cellular fibers. Quantitative readout is available at any time point. Cells become biological archives of their own gene expression biorxiv.org/content/10.1...

Major milestone for WeKwikGene! wekwikgene.wllsb.edu.cn now features 1,000+ ready-to-ship plasmids with 800+ more plasmids under QC. We've processed 1,000+ requests & distributed 3,000+ plasmids worldwide.

Our blue FP Electra2 shows excellent brightness & localization in soil-dwelling amoeba D. discoideum as fusion marker, with performance comparable to the golden standard mTagBFP2, all plasmids for Electra2 are available from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

The hCRAFi-CCR2 sensor reports chemokine release in the mouse visual cortex after LPS challenge using two-photon microscopy www.nature.com/articles/s41.... Plasmids and corresponding test AAVs can be requested from WeKwikGene for free wekwikgene.wllsb.edu.cn/publications...

hCRAFi-CCR2 is a new chemokine receptor activation-based fluorescent indicator for monitoring spatiotemporal dynamics of CCR2 ligands, including human/mouse CCL2, and CCL7, as well as mouse CXCL1, in culture and in vivo www.nature.com/articles/s41...

PSmOrange3 works well for long-term mitochondria tracking in cultured mammalian cells. Plasmids for PSmOrange3 can be requested for free from @WeKwikGene here wekwikgene.wllsb.edu.cn/publications...

Temporal domain multiplexing enables multiplexed imaging in a single channel with spectrally similar FPs using per-pixel unmixing based on differences in photobleaching rates. To facilitate this approach, we developed new far-red FPs www.biorxiv.org/content/10.1...

mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

Using mBaoJin derivative, Peng Zou lab engineered highly photostable green eFRET GEVI, named Vega, which exhibits more than 20-fold slower photobleaching than Ace-mNeon2, and allows for long-term voltage imaging in brain slice and in vivo in mice biorxiv.org/content/10.1...

Xue Han lab from BU used ElectraOFF to achieve routine cellular-resolution imaging over tens of minutes, occasionally up to eighty minutes, in behaving mice with minimal signal loss across neuron types and brain regions www.biorxiv.org/content/10.1...

Hour-long voltage imaging in behaving mice! Capitalizing on the high photostability and brightness of mBaoJin, we developed eFRET GEVIs, named ElectraOFF, which extends reliable single spike detection at cellular resolution beyond one hour biorxiv.org/content/10.1...

Due to its high chemical stability and brightness, mBaoJin is a perfect FP for Expansion Microscopy. Three-dimensional view of expanded HeLa cell coexpressing H2B-mTurquoise2 and Mito (COX8Ax2)-mBaoJin (www.nature.com/articles/s41...)

Soma-targeting of GECIs significantly improves image segmentation, as can be seen from these examples of single-plane 2-photon imaging for FRCaMPi, jRGECO1a, and their soma-localized variant from L2/3 neurons of primary visual cortex in awake mouse journals.plos.org/plosbiology/...

mScarlet3-H (aka mYongHong) makes a great pair with mBaoJin (nature.com/articles/s41...) for long-term live cell imaging. All plasmids can be requested for free from WeKwikGene wekwikgene.wllsb.edu.cn

A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution in mice and zebrafish journals.plos.org/plosbiology/...

All plasmids for FRCaMPis and SomaFRCaMPi are avalible from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

Great News & Views article by Ben Campbell covering recent development in FP field with focus on mScralet3-H (aka mYongHong) www.nature.com/articles/s41...

mScarlet3-H (aka mYongHong), a highly stable monomeric red fluorescent protein, works well for long-term super-resolution imaging, rapid tissue clearing, and CLEM www.nature.com/articles/s41...

We are very happy to see another orange flame lit up for our Electra2 plasmids on Addgene! Btw these plasmids can be also requested for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

New applications of Electra2! Patel et al. used Electra2 to screen for genetically modified nematodes to study dopamine signaling driving skin invasion (www.biorxiv.org/content/10.1...). All plasmids for Elctras can be requested from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

Whole-ExM for super-resolution imaging of entire embryonic and neonatal mouse bodies under conventional confocal microscopes pubs.acs.org/doi/full/10....

We are very excited to see the first orange flame lit up for our plasmid pHybrid-Electra2 on Addgene, which is designed for gene expression in E. coli and mammalian cells www.nature.com/articles/s41...

monArch, a near-infrared fluorescent voltage indicator, is developed via directed molecular evolution in HEK cells and characterized by several-fold higher baseline fluorescence compared to other Arch-based GEVIs. Plasmids are available from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

A Sensitive Soma-localized Red Fluorescent Calcium Indicator for Multi-Modality Imaging of Neuronal Populations In Vivo. It works well with wide-field imaging, two-photon microscopy, and fiber photometry biorxiv.org/content/10.1...

Year in review 2024 from @naturemethods ! I am very excited about the advancement in ultrastable monomeric GFPs we saw in 2024 and even more excited about what is coming next. nature.com/articles/s41...

Orthogonal RNA replication enables directed evolution and Darwinian adaptation in mammalian cells. Relevant plasmids are available from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

Novel genetically encoded red potassium indicators (RGEPOs) enable real-time visualization of both intracellular and extracellular potassium ion (K⁺) fluctuations in live tissues, allowing potassium dynamics to be imaged during seizures in mice for the first time www.biorxiv.org/content/10.1...

WeKwikGene is constantly improving to enhance the user experience. We’ve released a set of updates to improve navigation and allow users to view plasmids based on Lab and Publication, making material searches easier. wekwikgene.wllsb.edu.cn/plasmids/000...