Preprint 🧵! I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition! BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a www.biorxiv.org/content/10.6... (1/6)

Looking forward to sharing our work here!

What distinguishes dopamine vesicles from other synaptic vesicles? Our new preprint addresses this question: www.biorxiv.org/content/10.6... Huge shoutout to @Kenshiro Fujise for the stunning EM images! 🎉

🧪⚛️ Surface tension—driven by non-equilibrium currents not forces—in pattern formation leads to the same type of interface laws as in liquid foams: Plateau laws, von Neumann evolution; and a new emergent phenomenon: domain splitting akin to cell division. nature.com/articles/s4156…

At ASCB this year in Philly? Will speak about MT lattice spacing in cells and neurons 🙂 See info below..

How do cells recognize matrix topography? In this preprint we show that α5β1-integrin fibrillar adhesions rapidly form in response to topographic cues like electrospun nanofibers- no actomyosin contractility, focal adhesions or fibronectin secretion needed doi.org/10.1101/2025...

Please join me for the upcoming talk in the neurocolloquium!

Listened to the talk by the first author at SfN and was pleasantly surprised to see so many synaptic proteins, including my all-time favorite: synaptojanin-1!

Thrilled to share "in situ APEX activation" (iAPEX), a proximity labeling technology for subcellular #proteomics applied to primary #cilia. iAPEX uses an enzymatic cascade, in which a D-amino acid oxidase locally produces H2O2 to activate ascorbate peroxidase. www.nature.com/articles/s41...

If you’re at #sfn25 you definitely don’t want to miss this nanosymposium on cilia, tomorrow from 1-4pm! Come learn about neuronal cilia, they do some pretty cool stuff!

'Redefining dopaminergic synapses beyond the classical paradigm' Kenshiro Fujise, Jaya Mishra, Nasser Karmali & Nisha M. Rafiq @nishamrafiq.bsky.social www.cell.com/trends/neuro...

what a beauty!!

Looking forward to speak here!

Do you like following rules… but also breaking them? So does your dopaminergic synapse! 😎🧠 Our new Trends in Neurosciences review dives into the non-classical side of dopamine signaling, notably the tools reshaping how we see it (CLEM, cryo-CLEM, serial EM + more).

what an animation!

New paper alert! I’m very excited to share our paper published in @embojournal.org! @pdc-lab.bsky.social @yaleneuro.bsky.social

@anbangdai.bsky.social et al. @pdc-lab.bsky.social report the localization of BLTP2 in different human cell types & reveal binding partners that mediate its tethering to the PM & to PM-connected structures including certain #endosomes & #macropinosomes undergoing fusion rupress.org/jcb/article/...

Very happy that the first article from my postdoc work in the Tomancak lab is now published @PNAS! www.pnas.org/doi/10.1073/.... We studied the self-organization of actin in aggregates made from Hydra cells. Thread below (1/9)

Cilia in the brain! Depiction on angular and orientation information across different parts of the mouse brain...

Did you know that Bioimage Archive has a specific database for people to share AI-ready imaging datasets? I did not until @yanlanmao.bsky.social told me! Please deposit! If people regularly submitted, it would have a huge collective impact on bioimage analysis. www.ebi.ac.uk/bioimage-arc...

EU augments opportunities for scientists around the world to launch or re-launch their careers in Europe.

Today, FutureHouse is releasing the first dedicated AI Agents for Science, via our Platform. These agents are able to perform a wide variety of scientific tasks better than humans. With the platform, we are bringing these capabilities to the wider community. 1/2

A package of research papers from the MICrONS (Machine Intelligence from Cortical Networks) consortium in Nature and Nature Methods provides a detailed map of mouse brain cell structure and connections, offering insights into how they relate to activity in the mouse brain. 🧪

We're live! @inikon.bsky.social used modular proximity labeling to identify ciliary EV cargo in #celegans. This EV-TurboID approach can be applied in cell- & tissue-specific manners to define the composition of distinct EV subtypes, a major challenge of the EV field. www.nature.com/articles/s41...

Eid Mubarak! May this special day bring peace, joy, and blessings to you and your loved ones. 😊

Post doc in Europe? 🇪🇺🔬💡 It’s open to anyone in the 🌍 Call open 9 April 25 marie-sklodowska-curie-actions.ec.europa.eu/actions/post...

Constrained roads to complex brains Neural development and brain circuit evolution converged in birds and mammals www.science.org/doi/10.1126/...

Very happy to share our last preprint where we investigated the vesicular organization of dopamine hub synapses with cryo-CLEM. It is a brain structure that emerges as an important regulator of striatal activity. Many thanks to David Perrais, Etienne Herzog and the lab of @fronzeslab.bsky.social !

"DA vesicles are bigger and less round" in synaptosomes, beautiful cryoEM images! We too showed this in iPSC-derived DA neurons, of course using just regular EM 😃 See link here: www.nature.com/articles/s41... Exciting times for non-classical synapse!

Neuronal cilia and ubiquitination

Our paper on the phase separation of synaptophysin+ and dopamine+ vesicles, as well as their co-existence in iPSC-derived DA neurons, is now online! 🤓 www.nature.com/articles/s41...

Our review on the emerging roles for primary cilia in synapses and neurological disorders is now online! 😃https://www.sciencedirect.com/science/article/pii/S0962892424002319